| Project/Area Number |
09041100
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Field |
Bioorganic chemistry
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| Research Institution | The University of Electro-communications |
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Principal Investigator |
NIWA Haruki The University of Electro-communications Applied Physics and Chemistry Professor, 電気通信学部, 教授 (20135297)
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| Co-Investigator(Kenkyū-buntansha) |
OHMIYA Yoshihiro Shizuoka University Department of Biochemistry Associate Professor, 教育学部, 助教授 (20223951)
MAKI Shojiro The University of Electro-communications Applied Physics and Chemistry Reserach Associate, 電気通信学部, 助手 (20266349)
HIRANO Takashi The University of Electro-communications Applied Physics and Chemistry Associate Professor, 電気通信学部, 助教授 (20238380)
OHBA Nobuyosi Yokosuka City Museum Curator, 学芸員 (60100153)
井上 敏 チッソ(株), 横浜研究所, 主任研究員
中村 英士 名古屋大学, 大学院・生命農学研究科, 教授 (90217878)
MEYERーROCHOW ブイ ビー オウル大学, 教授
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1997: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | New Zealand / luminous organism / Latianeritoides / luciferin / luciferase / m-RNA / c-DNA / cloning / Lafia / C-DNA / Arachnocampa / Latia / c-DNA / Octochaetus |
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| Research Abstract |
Molecular base on bioluminescence of fresh water limpet-like snails Latia neritoides in New Zealand was investigated. Rapid and convenient method for purification of the Latia luciferase was established. (1) The Latia luciferase was found to be an N-linked glycoprotein, and to exhibit bioluminescence activity without any cofactor. The size exclusion chromatography analysis coupled with SDS-PAGE analysis and MALDI-TOF mass spectrometry indicated that the bioluminescent active Latia luciferase is a homohexamer, whose molecular weight is ca. 180,000 and the molecular weight of the non-bioluminescent subunit protein is ca. 31,000. (2) Although the natural Latia luciferin has an enol formate group, the formyl one was found to be not essential functionality, but enol acetate has also bioluminescence activity, indicating that the bioluminescence system of Latia is completely different from bacteria's ones believed previously and that the Latia luciferase has also an esterase activity. The formation of enolate anion in the Latia luciferase may be essential initial event in the bioluminescence reaction. (3) The Latia m-RNA was extracted from live specimens, from which Latia c-DNA library was constructed. Now cloning of the luciferase c-DNA is in progress.
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