Genetic diversity and adaptation among Southeast Asians

• Project/Area Number: 09041148
• Research Category: Grant-in-Aid for Scientific Research (B).
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 人類学(含生理人類学)
• Research Institution: The University of Tokyo
• Principal Investigator: ISHIDA Takafumi Graduate School of Science, Univ. Tokyo, Assoc. Prof., 大学院・理学系研究科, 助教授 (20184533)
• Co-Investigator(Kenkyū-buntansha): UEDA Shintaroh Graduate School of Science, Univ. Tokyo, Assoc. Prof., 大学院・理学系研究科, 助教授 (20143357) ; TIWAWECH Dan 国立がんセンター, 研究部, 研究員 ; SOEMANTRI Au ディポネゴロ大学, 医学部, 教授 ; MOHD Nizam I マレーシア理科大学, 医学部, 助教授 ; POOKAJORN Su シラパコン大学, 考古学部, 教授
• Project Period (FY): 1997 – 1999
• Project Status: Completed (Fiscal Year 1999)
• Budget Amount: ¥9,900,000 (Direct Cost: ¥9,900,000); Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000); Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 1997: ¥3,600,000 (Direct Cost: ¥3,600,000)
• Keywords: Southeast Asia / Cell bank / DNA bank / ancient DNA / Leukemia / Malaria / ミトコンドリア / DNAマーカー / LHON / 狩猟採集民 / Tリンパ腫 / 遺伝学 / 人類学

Research Abstract

The aims of this study are to find out human diversity, to prove the presence of the genetic adaptation based on the genetic diversity. For these, we have studied molecular bases for the short stature of tropical rain forest dwellers and genetic traits resistance to malarial diseases. During the study, we have collected biological materials and established cell lines from several Southeast Asian human populations.
1. Field study: for the population genetic study, blood sampling was done for Akha; Lisu and Mlaburi. A total of 350 specimens were collected and 300 cell lines were established.
2. Duffy blood group of which antigen is a receptor for vivax malaria was screened serologically and molecularly among these populations. Specimens of weakly reacted type were identified, whereas, Fy allele was not found.
3. As for the relationships between SAO (Southeast Asian ovalocytosis) and malaria species, difference in the resistance to malaria by the species was not observed.
4. Epstein-Barr virus (EBV) associated T-lyamphoma was surveyed by virologically in Southern Thailand ; the presence of EBV positive T-cells in the peripheral blood was confirmed.

Research Products

• [Publications] Kimura et al.: "Twenty base pain deleting in eryphrocy to band 3 protein gene asspansible for Southeast Asian ovalocytoszs is not common among Southeast Asian"Human Biology. 70. 973-1000 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ishida et al.: "Prelimtmary report on the short stature of Southeast Asian forest dwellers the Manni, in Southern Thailand"South Asian J. Trop. Med. Publ. Health. 29. 62-65 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fiujimoto et al.: "The gene for mesomelic dyoplasia Kantaptia type is mapped to chrammornt 2g24-32"J. Hum, Genat.. 43. 32-36 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shimizu et al.: "sero-and molecular-typing of Duffy blood group in Southeast Asians and Occaniano"Hum. Biol.. 72. (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Oota et al.: "Interdisciplimary perspectives on the origin of the Japanese"International Research Center for Japanese Studies. 25-41 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kimura, M. et al.: "Twenty seven base pair deletion in erythrocyte band 3 protein gene responsible for Southeast Asian ovalocytosis is not common among Southeast Asian."Human Biology. 70. 993-1000 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ishida. T. et al.: "Preliminary report on the short stature of Southeast Asian forest dwellers, the Manni, in Southern Thailand."Southeast Asian J. Trop. Med. Publ. Health. 29. 62-65 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fujimoto, M. et al.: "The gene for mesomelic dysplasia Kantaptra type is mapped to chromosome 2q24-32."J. Hum. Genet.. 43. 32-36 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shimizu, Y. et al.: "Sero- and molecular-typing of Duffy blood group in Southeast Asians and Oceaniams : demonstration of cryptic genotypes by the PCR-RFLP study."Humann Biology. 72 : (in press). (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oota, H. et al.: "Ancient DNA : a new strategy for studying population history. In. Omoto (ed.) Interdicsiplinary perspectives on the orogin of the Japanese."International Research (enter for Japanese studies). 25-41 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oota, H, et al.: "Ancient DNA a new strategy for studying population history"International Symposium. Int. Res. Center Jpn. studies. 25-41 (1999)
• [Publications] Takao, S, et al.: "Seroprevalence of Human T-lymphotupic virus type 1 in Papua New Guinea and Irian Jaya"Journal of Clinical Virology. 16-2. 127-131 (2000)
• [Publications] Shimizu, Y, et al.: "Sero- and Molecular-typing of Duffy Blood group in Southeast Asians and Oceanians"Human Biology. 72. (2000)
• [Publications] Danai Tiwawech and Takafumi,Ishida: "Presence of Ig A and IgG antibodies against Eqstein-Barrvirus early antigen in Thai hasopharyngeal cancer patients" Thai Cancer Journal. 22. 68-75 (1998)
• [Publications] T.Ishida et al.: "Preliminary report an the shore stature of Southeast Asian 80rest dwellers,the Manni,in Southern Thailand" Southeast Asian J.Trop.Med.Publ.Health. 29. 62-65 (1998)
• [Publications] M.Kimura et al.: "Twenty-seven base pair deletion in erythrocyta band 3 protein gene responsible for Southeast Asian ovabcyfosis is not common amory Sautheast Asians" Human Biology. 70. 933-1000 (1998)
• [Publications] Shimizu, Y.et al.: "Serotyping of Duffy blood group in several thai ethnic groups" Southeast Asian J.Trup.Med.Publ.Health. 28-1. 32-35 (1997)
• [Publications] M.Fujimoto et al.: "The gene for mesomelic dusplasia Kantaptia type is mapped to chromosome 2g 24-32" J.Hum.Genet.43.
• [Publications] Ishida, T.et al.: "Preliminary report on the short stature of Southeast Asian forest dwellers,the Monni" Southeast Asian J.Trup.Med.Pabl.Health. 29-1.