| Project/Area Number |
09041179
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Nagoya University |
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Principal Investigator |
KAWAMOTO Fumihiko Nagoya University, School of Medicine, Asso.Prof., 医学部, 助教授 (40115556)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Motoshi School of Medicine, Assis.Prof., 医学部, 講師 (80236017)
IWAI Kuni Jichi Med.Sch., Res.Assoc., 医学部, 助手 (50271226)
KIMURA Masatsugu Osaka City Univ., Res.Assoc., 医学部, 助手 (60195378)
MATSUOKA Hiroyuki Jichi Med., Assoc.Prof., 医学部, 助教授 (10173816)
ISHII Akira Jichi Med., Prof., 医学部, 教授 (40012752)
山本 敏充 名古屋大学, 医学部, 助手 (50260592)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | malaria / field survey / Southeast Asia / China / Plasmodium malariae / new variant / ribosomal RNA gene / G6PD deficiency / タイ / インドネシア / ミャンマー / ベトナム / ヒトマラリア / 遺伝子診断 / ラオス |
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| Research Abstract |
1. During 1997-1998, field surveys of malaria were conducted in Thailand, Indonesia, Myanmar, Laos, Vietnam and China by using an acridine orange staining. A total of about 13000 volunteers were examined in these countries, and malaria patients were detected in about 2000.
2. PCR-based diagnoses were further examined in a part of malaria-positive samples, and a new variant form of P.malariae was detected from China and Southeast Asia. This variant form possessed mutated sequence in the ribosomal RNA gene with 19-bp deletion at the target region.
3. The anterior part in the target sequence of the new variant form was the same with those of P.vivax and P.knowlesi, while its posterior part was corresponding to that of P.malariae. As this variant form was not found in Africa, it is probable that the variant form may distribute only in East Asia. All of patients infected with this variant form were mix-infected with other malaria species. For further studies on molecularly and clinically, it is essential to find patients infected singly with this variant form.
4. Field trials of a rapid test for G6PD deficiency were also performed with a rapid diagnosis of malaria on-site. By using these two rapid tests, primaquine was prescribed safely to malaria patients infected with P.vivax and gametocytes of P.falciparum.
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