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Surveys on new human malaria in South East Asia

Research Project

Project/Area Number 09041179
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research Field 寄生虫学(含医用動物学)
Research InstitutionNagoya University

Principal Investigator

KAWAMOTO Fumihiko  Nagoya University, School of Medicine, Asso.Prof., 医学部, 助教授 (40115556)

Co-Investigator(Kenkyū-buntansha) SUZUKI Motoshi  School of Medicine, Assis.Prof., 医学部, 講師 (80236017)
IWAI Kuni  Jichi Med.Sch., Res.Assoc., 医学部, 助手 (50271226)
KIMURA Masatsugu  Osaka City Univ., Res.Assoc., 医学部, 助手 (60195378)
MATSUOKA Hiroyuki  Jichi Med., Assoc.Prof., 医学部, 助教授 (10173816)
ISHII Akira  Jichi Med., Prof., 医学部, 教授 (40012752)
山本 敏充  名古屋大学, 医学部, 助手 (50260592)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywordsmalaria / field survey / Southeast Asia / China / Plasmodium malariae / new variant / ribosomal RNA gene / G6PD deficiency / タイ / インドネシア / ミャンマー / ベトナム / ヒトマラリア / 遺伝子診断 / ラオス
Research Abstract

1. During 1997-1998, field surveys of malaria were conducted in Thailand, Indonesia, Myanmar, Laos, Vietnam and China by using an acridine orange staining. A total of about 13000 volunteers were examined in these countries, and malaria patients were detected in about 2000.
2. PCR-based diagnoses were further examined in a part of malaria-positive samples, and a new variant form of P.malariae was detected from China and Southeast Asia. This variant form possessed mutated sequence in the ribosomal RNA gene with 19-bp deletion at the target region.
3. The anterior part in the target sequence of the new variant form was the same with those of P.vivax and P.knowlesi, while its posterior part was corresponding to that of P.malariae. As this variant form was not found in Africa, it is probable that the variant form may distribute only in East Asia. All of patients infected with this variant form were mix-infected with other malaria species. For further studies on molecularly and clinically, it is essential to find patients infected singly with this variant form.
4. Field trials of a rapid test for G6PD deficiency were also performed with a rapid diagnosis of malaria on-site. By using these two rapid tests, primaquine was prescribed safely to malaria patients infected with P.vivax and gametocytes of P.falciparum.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] M.Kimura et al.: "Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene." Parasitology International. 46. 91-95 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Stable patterns of allelic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern Vietnam." J.Eukaryotic Microbiology. 45. 131-136 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Z.Mian et al.: "High Prevalence of Plasmodium malariae and P.ovale in malaria patients along the Thai-Myanmar Border, as revealed by acridine orange staining" Tropical Medicine & International Health. 3. 304-312 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from southwestern Brazilian Amazon." Am.J.Tropical Medicine & Hygiene. 59. 474-480 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Allelic diversity at the merozoite surface protein-1 (MSP-1) locus in natural Plasmodium falciparum populations : A brief overview." Mem.Inst.Oswald Cruz. 93. 631-638 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Q.Liu et al.: "Sequence variation in small subunit ribosomal RNA gene of Plasmodium malariae and its local prevalence in Sichuan, China." J.Clin.Microbiology. 36. 3378-3381 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kimura et al.: "Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene." Parasitology International. 46. 91-95 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Stable patterns of allelic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern" J.Eukaryotic Microbiology. 45. 131-136 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Z.Mian et al.: "High Prevalence of Plasmodium malariae and P.ovale in malaria patients along the Thai-Myanmar Border, as revealed by acridine orange staining and PCR-based diagnoses" Tropical Medicine & International Health. 3. 304-312 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from southwestern Brazilian Amazon" Am.J.Tropical Medicine & Hygiene. 59. 474-480 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Allelic diversity at the merozoite surface protein-1 (MSP-1) locus in natural Plasmodium falciparum populations : A brief overview." Mem.Inst.Oswald Cruz. 93. 631-638 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Q.Liu et al.: "Sequence variation in small subunit ribosomal RNA gene of Plasmodium malariae and its local prevalence in Sichuan, China." J.Clin.Microbiology. 36. 3378-3381 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.U.Ferreira et al.: "Allelic diversity in the merozoite surface protein-1 and epidemiology of multiple clone Plasmodium falciparum infections in northern Tanzania." J.Parasitology. 84. 1286-1289 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I.S.Tantular et al.: "Field trials of a rapid test for G6PD deficiency in combination with a rapid diagnosis of malaria." Tropical Medicine & International Health (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I.S.Tantular et al.: "Field trials of a rapid test for G6PD deficiency in combination with a rapid diagnosisof malaria." Tropical Medicine & International Health. (In press). (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.U.Ferreira et al.: "Stable patterns of allelic diversity at the merozoite surface protein-1 of Plasmodium falciparum in clinical isolates from the southern Vietnam." J.Eukaryotic Microbiology. 45. 131-136 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Z.Mian et al.: "High Prevalence of Plasmodium malariae and P.ovale in malaria patients along the Thai-Myanmar Border,as revealed by acridine orange staining" Tropical Medicine & International Health. 3. 304-312 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.U.Ferreira et al.: "Allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from southwestern Brazilian Amazon." Am.J.Tropical Medicine & Hygiene. 59. 474-480 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.U.Ferreira et al.: "Allelic diversity at the merozoite surface protein-1(MSP-1)locus in natural Plasmodium falciparum populations: A brief overview." Mem.Inst.Oswald Cruz. 93. 631-638 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Q.Liu et al.: "Sequence variation in small subunit ribosomal RNA gene of Plasmodium malariae and its local prevalence in Sichuan,China." J.Clin.Microbiology. 36. 3378-3381 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Zhou, M.: "High prevalence of Plasmodium malariae and P.ovale in malaria patients along the Thai-Myanmar Border, as revealed by acridine orange staining and PCR-based diagnoses." Trop.Med.& Int.Health. 3(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Ferreira, M.U.: "Stable patterns of allelic diversity at the merozoite surface protein-1 locus of Plasmodium falciparum in clinical isolates from Southern Vietnam." J.Eukaryotic Microbiol.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 川本 文彦: "アクリジンオレンジ染色によるマラリアの迅速診断法。" 臨床検査. 42. 243-247 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kaneko, O.: "Plasmodium falciparum:Allelic variation in the merozoite surface protein 1 gene in wild isolates from Southern Vietnam." Exp.Parasitol.86. 45-57 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kimura, M.: "Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene." Parasitol.Int.46. 91-95 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wongsrichanalai, C.: "In vitro susceptibility of Plasmodium falciparum isolates in Vietnam to artemisinin derivatives and other antimalarials." Acta Tropica. 63. 151-158 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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