| Project/Area Number |
09044116
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Applied optics/Quantum optical engineering
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| Research Institution | Kitami Institute of Technology |
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Principal Investigator |
KAMEMARU Shun-ichi Kitami Institute of Technology, Fac.of Engineering, Professor, 工学部, 教授 (60175289)
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| Co-Investigator(Kenkyū-buntansha) |
UNO Katsuhiro Ibaraki University, Fac.of Engineering, Lecturer, 工学部, 講師 (10280710)
KIYONO Hiroshi Osaka University Research Institute For Microbial Diseases, Professor, 微生物病研究所, 教授 (10271032)
MIURA Noriaki Kitami Institute of Technology, Fac.of Engineering, Assistant Professor, 工学部, 助教授 (30209720)
KUBOTA Toshio Ibaraki University, Fac.of Engineering, Lecturer, 工学部, 講師 (40143143)
FUJII Kan-ichi Ibaraki University, Fac.of Engineering, Professor, 工学部, 教授 (00054354)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | Immunoassay / Automated measurement of cell / Fluorescent dye / Cytokin / Image processing / Fluorine-containing compound / White light laser |
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| Research Abstract |
Results from the International Scientific Research, Joint Research, during 1998 are as follows ;
1) Automated observation of cells : Auto-focus mechanism and auto-scanning of the cell stage systems were developed. Manually adjusting systems were improved to automated ones by the technique using a feedback control method through observed image signals.
2) Preparation of new fluorescent dyes and development of staining protocol : Quantitative reaction of trifluoroethyl esters of fluorine-containing carboxylic acids to proteins was found. Microscopic infrared mapping of carbon-fluorine bonds could beutilized complementarily with ILISPOT assay. These results are only realized by a prototype system, however, those would soon be realized by a clinical inspection system.
3) Processing of the observed imaging signals : Recognition technique for identifying pseudo-spot with uniform intensity from real spot were developed using soft ware algorithm. Using this technique, high S/N ration signals were obtained without changing the hardware part of the proposed system.
4) Discussion of a laser as a light source : Throughout theoretical discussions and simulations, the idea of 40% down sizing of the conventional laser was proposed as a new illuminating light source for the smaller system in a clinical field.
^<**>Further research in the next project : During the experiments in this project, Dr.Mirov, investigator, found that tomographic imaging of the cells could be realized using an illumination by a tunable wavelength laser. Realization of the system, called a laser Raman microscope, has great possibility for an application to an Immunoassay System in the near future.
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