| Project/Area Number |
09044167
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
生物・生体工学
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| Research Institution | Osaka University |
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Principal Investigator |
MUROOKA Yoshikatsu Osaka Univ.Eng., Professor, 大学院・工学研究科, 教授 (60029882)
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| Co-Investigator(Kenkyū-buntansha) |
BAYANI Espir フィリピン大学, 生物工学研究所, 助教授
MADE Sri Pra インドネシア国立科学研究所, 所長
OZAWA Takashi Osaka Pref.Univ.Agric., Assoc.Prof., 農学部, 助教授 (20152481)
MINAMISAWA Kiwamu Tohoku Univ., Professor, 遺伝生態研究センター, 教授 (70167667)
HIGASHI Shiro Kagoshima Univ.Sci., Professor, 理学部, 教授 (60041216)
PRANA Made Sri LIPI,Head
ESPIRITU Bayani UPLB,Assoc.Prof.
ESPIRITU Bay フィリピン大学, 生物工学研究所, 助教授
SRIPRANA Mad インドネシア国立科学研究所, 生物工学センター, 所長
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1998: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1997: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | nitrogen-fixation / nodule / Southeast Asia / symbiont / leguminous tree / rhizobia |
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| Research Abstract |
1)Thai researchers isolated various kinds of rhizobia from nodules of soy bean in acidic soils in Thailand. These strains grow at pH 3.5 or at 4.0. By collaboration with Osaka University, three acid tolerant strains of Bradyrhizobium were succeeded in transformation to tagging with the Green Fluorescence Protein (GFP). These colonies on the plates showed green fluorescence under the UV exposure.
2)Indonesian researchers isolated nitrogen-fixing rhizobia from nodules of leguminous trees in Indonesia. These bacteria were classified by collaboration with Osaka University, Hiroshima University, and Kagoshima University. Bacterial strains isolated from root nodules on Acacia mangium at various locations in Indonesia were classified to Bradyrhizobium elkanji. Bacterial strains isolated from root nodules on Paraserianthes falcataria at various locations in Indonesia were classified into Bradyrhizobiwn elkanil, Rizizobiwn tropici and strains between these species. An acid tolerant strain, Bradyrhizobiwn sp. BTCC-B64 was evaluated as nitrogen fertilizer substitute. The infection of the strains were effective on the nitrogen fixation which performed by the total dried weight of plants, roots and nodules.
3)Free living nitrogen-fixing bacteria, Azotobacter sp. or cellulolytic fungi of Trichoderma sp. isolated in Philippines were used as inoculant in cattle manure-rice hull compost. The inoculation with nitrogen-fixing bacteria improved the degradation and nutrient content of the materials and enhance the inoculant load in the compost.
4)The niflHDKTY Peron from a nitrogen fixing gram positive bacterium, Paenibacillus azotofixans were cloned and characterized by Malaysian researchers. Azospirillun was found to be an effective bioenhancer for banana and oil palm seedings, and vegetatable soybean as well as a biofertilizer for banana.
5)Survivability of the inoculant of Bradyrhizobium japonicum in acid paddy soil in Vietnam was investigated.
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