| Project/Area Number |
09044199
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SUGIMOTO Chihiro HOKKAIDO UNIV., GRADUATE SCHOOL OF VET.MED., ASSOC.PROF., 大学院・獣医学研究科, 助教授 (90231373)
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| Co-Investigator(Kenkyū-buntansha) |
NENE Vishnash INT.LIVESTOCK RES.INST., RESEARCHER, 研究員
BISHOP Richard INT.LIVESTOCK RES.INST., RESEARCHER, 研究員
MORZARIA Subhash INT.LIVESTOCK RES.INST., RESEARCHER, 研究員
OHASHI Kazuhiko HOKKAIDO UNIV., GRADUATE SCHOOL OF VET.MED., INST., 大学院・獣医学研究科, 助手 (90250498)
ONUMA Misao HOKKAIDO UNIV., GRADUATE SCHOOL OF VET.MED., PROF., 大学院・獣医学研究科, 教授 (70109510)
VISHNASH Nen 国際家畜研究所, 研究員
RICHARD Bish 国際家畜研究所, 研究員
SUBHASH Morz 国際家畜研究所, 研究員
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 1998: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1997: ¥7,700,000 (Direct Cost: ¥7,700,000)
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| Keywords | GENOME ANALYSIS / THEILERIA / BAC LIBRARY / PROTOZOA / EXPRESSED SEQUENCE TAG |
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| Research Abstract |
This project was carried out to analyze genomes of Theileria parasites Eight hundred cDNA clones of Theileria parva was partially sequences to use as expressed sequence tags (ESTs). Some of them were mapped on chromosomes of T.parva. A bacterial artificial chromosome (BAC) library, each of which clones contained 50-200 kilobase pairs of T.parva chromosome fragments, was constructed.
The library consisted of 3600 clones that theoretically covered entire T.parva genome. BAC clones that contained gene(s) essential for parasite growth including sporozoite surface protein were identified, which will be useful to analyze genome structures flanking these genes.
4 chromosomes of T.sergenti were identified. Size of each chromosome and total genome size of this species was almost same as those of T.parva. Comparative mapping analysis of several homologous genes on T.parva and T.sergenti chromosomes revealed that syntenies among some of the genes are not conserved, which indicated difference of genome structures between these two Theileria species.
Although further studies are required to determine whole chromosome structure of T.parva, the results of this project provide a basal information about genome structures of Theileria parasite by establishing BAC library, obtaining ESTs, and comparing two theilerial genomes.
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