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Function analysis of soxgenes in the development and differentiation of digestion

Research Project

Project/Area Number 09044233
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTOKYO METOROPOLITAN UNIVERSITY

Principal Investigator

YASUGI Sadao  Tokyo Metropolitan University, Gr. Sch. Sci., Prof., 大学院・理学研究科, 教授 (70011591)

Co-Investigator(Kenkyū-buntansha) SCOTTING P.j.  Inst. genetics, England, Lecturer, 遺伝学研究所, 講師
FUKUDA Kimiko  Tokyo Metropolitan University, Gr. Scho. Sci., Assistant Prof., 大学院・理学研究科, 助手 (40285094)
SAIGA Hidetoshi  Tokyo Metropolitan University, Gr. Scho. Sci., Associate Prof., 大学院・理学研究科, 助教授 (60131918)
MATSUSHITA Susumu  Tokyo Women's Med. College, Fac. Med., Lecturer, 医学部, 講師 (50165809)
SCOTTING P.J  イギリスノッティンガム大学, 医学部, 講師
REX Maria  ノッティンガム大学, 医学部(イギリス), 助手
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsdigestive organ / chick embryo / transcription factor / Sox genes / Cdx / stomach / intestine / Sox遺伝子 / 消化管 / 細胞分化 / 上皮-間充織相互作用 / 領域決定 / ペプシノゲン遺伝子
Research Abstract

The present study intended to clarify the functions of Sox genes, which recently become known to play important roles in various processes of organogenesis, in the development and differentiation of vertebrate digestive organs.
Sox genes encode transcription factors having HMG domain and more than 20 genes have been cloned so far. We paid special attention to cSox2 and cSox21, which are expressed in the gut epithelium of chicken embryo. We analyzed in the present study their expression patterns, regulation of these genes by the mesenchyme, and functional analysis using gene transduction methods. Both genes are expressed in the epithelium of anterior digestive organs such as esophagus, proventriculus and gizzard. Their expression ceased when embryonic morphogenesis and differentiation come to end. Their posterior boundary of expression coincided with the anterior boundary o CdxA, a gene specifically expressed in the intestinal epithelium. Moreover, their expression was very low in the epithelium of newly formed proventricular glands.
Expression of cSox2 and cSox21 was shown to be regulated by the mesenchyme. When the intestinal epithelium was cultivated with gizzard mesenchyme some epithelial cells became positive to cSox2 and cSox21. Conversely, the expression of both genes was suppressed in stomach epithelium cultivated with intestinal mesenchyme.
Introduction and forced expression of cSox2 and cSox21 into intestinal epithelium never resulted in the suppression of CdxA, suggesting that some partner molecules are necessary for the suppression of target genes by cSox2 and cSox21 gene production.
Thus, in the present study, possible involvement of cSox2 and cSox21 in the organogenesis of the digestive organs was demonstrated. In addition, the basic data for further analysis of their function were accumulated.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Koike, T.: "Invitro analysis of mesenchymal influences on the differentiation of stomach ep : thelial cells of chicken embryo"Differentiation. 65. 13-25 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasugi, S.: "The mesenchymal factors requlating epithelial morphogenesis and differentia tion of the chiken stomach"Zoological Science. 17. 1-9 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Narita, T.: "BMPs are necessary for stomach gland formation in the chiken enbryo : A study using virally induced BMP-2 and Noggin edpression"Development. 127. 981-988 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sukegawa, A.: "The cancentric structure of the developing gut is vegulated by sonic hedge*og derived from endodermal epithelium"Development. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuda, K.: "Application of the efficient and specitic gene transfer systems and orgon wlture technigve for the eluciclation of mechanisms of e**thelial-meseuchymal interaction in the developing gut"Divelopment, Frooth and Differeutiation. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] KOIKE,T., et al.: "In vitro analysis of mesenchymal influences on the differentiation of stomach epithelial cells of chicken embryo."Differentiation. 65. 13-25 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YASUGI,S.: "The mesenchymal factors regulating epithlial morphogenesis and differentiation of the chicken stomach."Zoological Science. 17. 1-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] NARITA,T., et al.: "BMPs are necessary for stomach gland formation in the chicken embryo: A study using virally induced BMP-2 and Noggin expression."Development. 127. 981-988 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] SUKEGAWA,A., et al.: "The concentric structure of the developing gut is regulated by sonic hedgehog derived from endodermal epithelium"Development. in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] FUKUDA,K., et al.: "Application of the efficient and specific gene transfection systems and organ culture technique for the elucidation of mechanisms of epithelial-mesenchymal interaction in the developing gut."Dev., Growth & Differ. in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koike, T.: "Invitro analysis of mesenchymal influences on the differentiation of stomach epithelial cells of chicken embryo"Differentiation. 65. 13-25 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasugi, S.: "The mesenchymal factors regulating epithelial morphogenesis and differentiation of the chicken stomach"Zoological Science. 17. 1-9 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Narita, T.: "BMPs are necessary for stomach gland formation in the chiken embryo: A study using virally induced BMP-2 and Noggin expression"Development. 127. 981-988 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sukegawa, A.: "The concentric structure of the developing gut is regulated by sonic hedgellog derived from endodermal epithelium"Development. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fukuda, K.: "Application of the efficient and specific gene transfer systems and organ culture technique for the elucidation of mechanisms of ejithelial-mesenchsginal interaction in the developing gut."Development, Growth and Differentiation. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Narita,T.: "Sonic hedgehog expression in developing chicken digestive organs is requlated by epithelial-mesenchymal interactions" Develop,Growth & Differentiation. 40(1). 67-74 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto,K.: "Differential expression of fos and jun family members in the developing chicken gastro in testinal tract" Oncogene. 16. 1611-1616 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakamoto,N: "Analysis of temporal expression-pattern and cis-regulatory sequences of chicken popsinoger A and C" Biochemical and Biophysical Research Communications. 250. 420-424 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukuda,K.: "CFKBP/SMAP;A novel molecule involved in the requlation of smooth muscle differentiation" Development. 125. 3535-3542 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishii,Y.: "Pegion-specific expression of chicken Sox2 in the developing gut and lungepithelium:Regulation by epithelial-mesenchymal interactions" Developmental Dynamics. 213. 464-475 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsushita,S.: "Revelopmental changes in mucosubstances reveoled by immunostaining with antimacus monoclonal antibodies and lectin staining in the epithelium lining the segment from gizzarol to duodenum of the chick embryo" Journal of Anatomy. 193. 587-597 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akazome, Y.: "Ontogeny of the expression of messenger ribonucleic acid encoding lutropin receptor in chicken embryo" Zool.Sci.14. 363-367 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishii, Y.: "Early specification of intestinal epithelium in the chicken embryo : A study on the localization and regulation of CdxA expression" Deu.Growth Differ. 39. 643-653 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sato, K.: "Chicken keratin-19 : Cloning of cDNA and analysis of expression in the chicken embryonic gut" Deu.Growth Differ.39. 751-761 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 八杉 貞雄: "総合化の可能性に関する生物教育学会の対応と意見" 地学教育. 50. 261-264 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Narita, T.: "Sonic hedgehog expression in the developing chicken digestive organs is regulated by epithelial-meienchymal interactions" Deu.Growth Differ.40(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsmoto, K.: "Differential expression of fos and jun family members in the developing chicken gastrointestinal tract" Oncogene. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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