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STUDIES ON SPERM-EGG INTERACTIONSIN MAMMALIAN FERTILIZATION

Research Project

Project/Area Number 09044240
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionOKAZAKI NATIONAL RESEARCH INSTITUTE

Principal Investigator

MOHRI Hideo  NATIONAL INSTITUTE FOR BASIC BILOGY, OKAZAKI NATIONAL RESEARCH INSTITUTES, DIRECTOR GENERAL, 基礎生物学研究所, 所長 (70012268)

Co-Investigator(Kenkyū-buntansha) 岡部 勝  大阪大学, 遺伝情報実験施設, 教授 (30089875)
ISIJIMA Sumio  BIOL., LAB., FAC. BIOSCI. & TOKYO INT. TECH, RES. ASSOCIATE, 理学部, 助手 (70193315)
OKUNO Makoto  DEPT. BIOL, LAB., FAC. BIOSCI. & BIOTECH, TOKYO SINST. TECH, OKAZAKI NATIONAL RESEARCH INSTITUTES, RES. ASSOCIATE, 大学院・総合文化研究科, 助教授 (40143325)
小川 和男  岡崎国立共同研究機構, 基礎生物学研究所, 助教授 (30132731)
YANAGIMACHI R.  DEP. OF ANATOMY & REPROD. IO., UNIV. OF HAWAII, PORFESSOR
SAVAGE J.s.  DEP. BIOL., UNIV. NEW ORLEANS, ASSOCIATE PROFESSOR
SUAREZ S.s.  DEP. BIOMEDICAL SCI., CORNELL UNIV., PROFESSOR
STEWART・SAVA ジェイ  ニューオリンズ大学, 生物科学, 副教授
SUAREZ S.S.  コーネル大学, 獣医学部, 副教授
YANAGIMACHI アール  ハワイ大学, 医学部, 教授
SAVAGE J.STE  ニューオリンズ大学, 生物科学, 副教授
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1997: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsMammalian perm / hyperactivation / capacitation / flagellar motility / acrosome reaction / sperm-egg interactin / 透明帯通過
Research Abstract

The main results obtained for these three years are as follows.
(1) Analysis of the records on high speed video system indicated that hamster spermatozoa exhibit an increase in bend of the mid-piece region with an asymmetrical wave form at the beginning of hyperactivation following capacitation. Onset of the acrosome reaction caused a larger bend of the mid-piece region with a symmetrical wave form. Similar results were obtained with monkey spermatozoa, although little change was observed when spermatozoa of transgenic mouse lines, that accumulate mutated green fluorescence protein in the acrosome, were capacitated and acrosome-reacted.
(2) Hyperactivation was induced with procaine in hamster and bull spermatozoa. Lysolecithin also caused simultaneous hyperactivation in guinea pig spermatozoa.
(3) Mammalian spermatozoa show sequential changes in motility, namely resting, weakly active, activated and hyperactivated. Different specific proteins were phosphorylated at each step. Upon hyperactivation a 80kDa protein localized in the main part of sperm flagella was phosphorylated.
(4) CaィイD12+ィエD1 and cAMP affected both amplitude and wave form of sperm flagella. It was found that the redundant nuclear envelope in the base of the flagellum serves as a CaィイD12+ィエD1 store. The CaィイD12+ィエD1appears to be released to the mitochondria and to increase production of ATP to support hyperactivation.
(5) The spermatozoon passing though the zona pellucida exhibited flagella movement with large amplitude and symmetrical wave form. The movement would provide sufficient propulsive force to pass through the zona pellucida.
(6) Mechanism of motility Suppression by glucose was examined. It was found that two of light chains of dynein, motor protein for flagella motility, correspond to Tctex (t-complex) genes.
(7) In addition, fertilization and pregnancy following intracytoplasmic injection of sperm or spermatogenic cells in the mouse, etc., were also examined.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report

Research Products

(108 results)

All Other

All Publications (108 results)

  • [Publications] Kubo-Irie ,M. et al.: "Morphological evidence that spermatogenetic cells undergo apoptosis within testis of diapausing swallowtail pupa(Astrophaneura alcinous)"J.Exp.Zool.. 283. 194-201 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mohri, H. et al.: "Characterization of outer arm dynein in sea anemone, Anthopleura midori"Cell Motil, Cytoskel.. 44. 202-208 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mohri, H.: "Tubulin 30 years later"Cell Struct. Funct.. 24. 265-267 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Si, Y. and Okuno, M.: "Regulation of microtublue sliding by 36-kDa phosphoprotein in Hamster sperm flagella"Mol. Reprod. Dev.. 52. 328-334 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Si, Y. and Okuno, M.: "Role of tyrosine phosphorylation of flagellar proteins in hamster sperm hyperactivation"Biol. Reprod. 61. 240-246 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 石島純夫: "精子運動のメカニズム"日本受精着床学会雑誌. 14. 7-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishijima, S. et al.: "Comparative studies on the spermatozoan motility of Japanese fishes"Bull. Ocean Res. Inst. Univ. Tokyo. 33. 139-152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishijima, S. et al.: "Movement of Turritella spermatozoa : direction of propagation and chirality of flagellar bends"Cell Motil. Cytoskel.. 44. 85-95 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, S. et al.: "Mitochondrial thioredoxin reductase in bovine adrenal cortex : its purification, properties, nucleotide/amino acid sequences, and identification of selenocysteine"Eur. J. Biochem.. 204. 74-84 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inaba, K. et al.: "Tctex2-related outer arm dynein light chain is phosphorylated at activation of sperm motility"Biochem. Biophys. Res. Commum. 256. 177-183 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyado, K. et al.: "Requirement of CD9 on the egg plasma membrane for fertilization"Science. 287. 321-324 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hamada, Y. et al.: "Mutation in ankyrin repeats of the mouse notch2 gene induces early embryonic lethality"Development. 126. 7-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakanishi, T. et al.(Okabe, M.): "Real-time Observation of acrosomal dispersal from mouse sperm using GFP as a marker protein"FEBS Lett. 449. 277-283 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto,A. et al.(Okabe, M.): "Suppression of STAT5 function in liver,mammary glands, and T cells in cytokine-inducible SH2-containing protein 1 transgenic mice"Mol. Cell Biol. 19. 6396-6407 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato, M. et al.: "Efficient selection of transgenic mouse embryos using EGFP as a marker gene"Mol. reprod. Dev.. 54. 43-48 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Perry, A. C. et al.: "Mammalian transgenesis by intracytoplamic sperm injection"Science. 284. 1180-1183 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usui, N. et al.: "Ultrastructural changes in hamster spermatogenic cell nuclei after incorporation into homologous oocytes by electrofusion"Mole. Reprod. Dev.. 52. 66-73 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Perry, A. C. F. et al.: "A novel trans-omplementation assay suggests full mammalian oocyte activation is coordinated by multiple submembrane sperm components"Biol. Reprod. 60. 747-755 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yanagida, K. et al.: "Successful fertilization and pregnancy following ICSI and electrical oocyte activation"Human Reprod. 14. 1307-1311 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kishikawa, H., Tateno, H., and Yanagimachi, R.: "Fertility of mouse spermatozoa from cadavers maintained at 4^○C"J. Reprod. Feril.. 116. 217-222 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Cummins, J. M. et al.: "Fate of genetically marked mitochondrial DNA from apermatocytes microinjected into mouse zygotes"Zygote. 7. 151-156 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kasai, T., Hoshi, K and R. Yanagimachi: "Effect of sperm immobilisation and demembranation on the oocyte activation rate in the mouse"Zygote. 7. 187-193 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ho, H-C, Tnag C-Y, and Suarez, S. S.: "Three-dimensional structure of the Golgi apratus in mouse spermatids : a scanning electron microscope study"Anat. Rec.. 256. 189-194 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Revah, I. et al.: "The physiological state of bull sperm affects fucose and mannose binding properties"Bio. Reprod.. 62(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Visconti, P. E. et al.(Stewart-Savage, J.): "Roles of bicarbonate, cAMP and protein tyrosine phosphorylation on capacitating and the spontaneous acrosome reaction of hamster sperm"Bio. Reprod.. 61. 76-84 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Stewart-Savage, J. et al.: "Developmental Basis of phenotypic variation in egg production in a colonial ascidian : primary oocyte production versus oocyte development"Bio. Bull.. 196. 63-69 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suarez,S.S.: "The Male Gemete : From basic sience clinical applications."Cache Riever Press. 10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubo-Irie, M. et al. (Mohri, H): "Morphological evidence that spermatogenic cells undergo apoptosis within testis of diapausing swallowtail pupa (Atrophaneura alcinous)."J. Exp. Zool. 283. 194-201 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mohri, H. et al.: "Characterization of outer arm dynein in sea anemone, Antholeura midori."Cell Motil. Cytoskel. 44. 202-208 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mohri, H.: "Tubulin 30 years later"Cell Struct. Funct.. 24. 265-267 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Si, Y. and Okuno, M.: "Regulation of microtubule sliding by 36-kDa phosphoprotein in Hamster sperm flagella."Mol, Reprod. Dev. 52. 328-334 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Si, Y and Okuno, M: "Role of tyrosine phosphorylation of flagellar proteins in hamster sperm hyperactivation."Biol. Reprod. 61. 240-246 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishijima, S. et al.: "Comparative studies on the spermatozoan motility of Japanese fishes."Bull. Ocean Res. Inst. Univ. Tokyo. 33. 139-152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishijima, S. et al.: "Movement of turritella spermatozoa. Direction of propagation and chirality of flagellar bends."Cell Motil. Cytoskel.. 44. 85-95 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watabe, S. et al. (Ogawa, K.): "Mitochondrial thioredoxin reductase in bovine adrenal cortex: its purification, properties, nucleotide/amino acid sequences, and identification of selenocysteine"Eur. J. Biochem.. 204. 74-84 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inaba, K. et al. (Ogawa, K.): "Tetex2-related outer arm dynein light chain is phosphory-lated at activation of sperm motility."Biochem, Biophys. Res. Commun.. 256. 177-183 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyado, K. et al. (Okabe, M.): "Requirement of CD9 on the egg plasma membrane for fertilization."Science. 287. 321-324 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hamada, Y. et al. (Okabe, M.): "Mutation in ankyrin repeats of the mouse noich2 gene induces early embryonic lethality."Development. 126. 7-10 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakanishi, T. et al. (Okabe, M.): "Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein."FEBS Lett. 449. 277-283 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto, A. et al. (Okabe, M.): "Suppression of STAT5 functions in liver, mammary glands, and T cells in cytokine-inducible SH2-containing protein 1 transgenic mice."Mol, Cell Biol.. 19. 6396-6407 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato, M. et al. (Okabe, M.): "Efficient selection of transgenic mouse embryos using EGFP as a marker gene."Mol. Reprod. Dev.. 54. 43-48 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Perry, A. C. et al. (Okabe, M.): "Mammalian transgenesis by intracytoplasmic sperm injection."Science.. 284. 1180-1183 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usui, N. et al. (Yanagimachi, R.): "Ultrastructural changes in hamster spermatogenic cell nuclei after incorporation into homologous oocytes by electrofusion."Mole. Reprod. Dev.. 52. 66-73 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Perry, A. C. F. et al. (Yanagimachi, R.): "A novel trans-complementation assay suggests full mammalian oocyte activation is coordinated by multiple submembrane sperm components."Biol. Reprod.. 60. 747-755 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yanagida, K. et al. (Yanagimachi, R.): "Successful fertilization and pregnancy following ICSI and electrical oocyte activation."Human Reprod.. 14. 1307-1311 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kishikawa, H., Tateno, H. and Yanagimachi, R.: "Fertility of mouse spermatozoa from cadavers maintained at 4 ℃."J. Reprod. Feril.. 116. 217-222 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Cummins, J. M. et al. (Yanagimachi, R.): "Fate of genetically marked mitochondrial DNA from spermatocytes microinjected into mouse zygotes."Zygote. 7. 151-156 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kasai, T., Hoshi, K. and Yanagimachi, R.: "Effect of sperm immobilisation and demembranation on the oocyte activation rate in the mouse."Zygote. 7. 187-193 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ho, H-C, Tang, C-Y and Suarez, S. S.: "Three-dimensional structure of the Golgi apratus in mouse spermatids: a scanning electron microscope study."Anat. Rec.. 256. 189-194 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Revah, I. Et al. (Suarez, S. S.): "The physiological state of bull sperm affects fucose and mannose binding properties."Bio. Reprod.. 62(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Visconti, P.E. et al. (Stewart-Savage, J.): "Roles of bicarbonate, cAMP and protein tyrosine phosphorylation on capacitation and the spontaneous acrosome reaction of hamster sperm."Bio. Reprod.. 61. 76-84 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Stewart-Savage, J. et al.: "Developmental basis of phenotypic variation on egg production in a colonial ascidian : primary oocyte production versus oocyte development."Biol. Bull.. 196. 63-69 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubo-Irie, M. et al. (Mohri H.): "Morphological evidence that spermatogenic cells undergo apoptosis within testis of diapausing swallowtail pupa (Atrophaneura alcinous)"J. Exp. Zool.. 283. 194-201 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mohri, H. et al.: "Characterization of outer arm dynein in sea anemone, Anthopleura midori"Cell Motil. Cytoskel. 44. 202-208 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mohri, H.: "Tubulin 30 years later"Cell Struct. Funct.. 24. 265-267 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Si, Y. and Okuno, M.: "Regulation of microtubule sliding by 36-kDa phosphoprotein in Hamster sperm flagella"Mol. Reprod. Dev.. 52. 328-334 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Si, Y. and Okuno, M.: "Role of tyrosine phosphorylation of flagellar proteins in hamster sperm hyperactivation"Biol. Reprod.. 61. 240-246 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 石島純夫: "精子運動のメカニズム"日本受精着床学会雑誌. 14. 7-10 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishijima, S. et al.: "Comparative studies on the spermatozoan motility of Japanese fishes"Bull. Ocean Res. Inst. Univ, Tokyo. 33. 139-152 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishijima, S. et al.: "Movement of Turritella spermatozoa : direction of propagation and chirality of flagellar bends"Cell Motil. Cytoskel. 44. 85-95 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watabe, S. et al. (Ogawa K.): "Mitochondrial thioredoxin reductase in bovine adrenal cortex : its purification, properties, nucleotide/amino acid sequences, and idenification of selenocysteine"Eur. J. Biochem.. 204. 74-84 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Inaba, K. et al. (Ogawa,K.): "Tctex2-related outer arm dynein light chain is phosphorylated at activation of sperm motility"Biochem. Biophys. Res. Commun.. 256. 177-183 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyado, K. et al. (Okabe M.): "Requirement of CD9 on the egg plasma membrane for fertilization"Science. 287. 321-324 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hamada, Y. et al.: "Mutation in ankyrin repeats of the mouse notch2 gene induces early embryonic lethality"Development. 126. 7-10 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakanishi, T. et al. (Okabe M.): "Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein"FEBS Lett. 449. 277-283 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto, A. et al. (Okabe M.): "Suppression of STAT5 functions in liver, mammary glands, and T cells in cytokine-inducible SH2-containing protein 1 transgenic mice"Mol. Cell Biol.. 19. 6396-6407 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kato, M. et al. (Okabe M.): "Efficient selection of transgenic mouse embryos using EGFP as a marker gene"Mol. Reprod. Dev.. 54. 43-48 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Perry, A.C. et al. (Okabe M.): "Mammalian transgenesis by intracytoplasmic sperm injection"Science. 284. 1180-1183 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Usui, N. et al. (Yanagimachi R.): "Ultrastructural changes in hamster spermatogenic cell nuclei after incorporation into homologous oocytes by electrofusion"Mole. Reprod. Dev.. 52. 66-73 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Perry, A.C.F. et al. (Yanagimachi,R.): "A novel trans-complementation assay suggests full mammalian oocyte activation is coordinated by multiple submembrane sperm components"Biol. Reprod. 60. 747-755 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yanagida, K. et al. (Yanagimachi R.): "Successful fertilization and pregnancy following ICSI and electrical oocyte activation"Human Reprod. 14. 1307-1311 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kishikawa, H., Tateno, H. and Yanagimachi, R.: "Fertility of mouse spermatozoa from cadavers maintained at 4℃"J. Reprod. Feril. 116. 217-222 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Cummins, J.M. et al. (Yanagimachi, R.): "Fate of genetically marked mitochondrial DNA from spermatocytes microinjected into mouse zygotes"Zygote. 7. 151-156 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kasai, T., Hoshi, K and R. Yanagimachi: "Effect of sperm immobilisation and demembranation on the oocyte activation rate in the mouse"Zygote. 7. 187-193 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ho, H-C, Tnag C-Y, and Suarez, S.S.: "Three-dimensional structure of the Golgi apratus in mouse spermatids ; a scanning electron microscope study"Anat. Rec.. 256. 189-194 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Revah, I. Et al. (Suarez, S.S.): "The physiological state of bull sperm affects fucose and mannose binding properties"Biol. Reprod. 62(in press). (2000)

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      1999 Annual Research Report
  • [Publications] Visconti, P.E. et al. (Stewart-Savage, J.): "Roles of bicarbonate, cAMP and protein tyrosine phosphorylation on capacitation and the spontaneous acrosome reaction of hamster sperm"Biol. Reprod. 61. 76-84 (1999)

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      1999 Annual Research Report
  • [Publications] Stewart-Savage J. et al.: "Developmental basis of phenotypic variation in egg production in a colonial ascidian : primary oocyte production versus oocyte development"Biol. Bull. 196. 63-69 (1999)

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      1999 Annual Research Report
  • [Publications] Suarez, S.S.: "The Male Gemete : From basic science to clinical applications"Cache Riever Press. 10 (1999)

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      1999 Annual Research Report
  • [Publications] Kubo-Irie,M.et al.(Mohri,H.): "Morphological evidence that spermatogenic cells undergo apoptosis within testis of diapausing swallowtail pupa (Atrophaneura alcinous)" Journal of Experimental Zoology. 283. 194-201 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kubo-Irie,M.et al.(Mohri,H.): "Morphological changes in eupyrene and apyrene spermatozoa in the reproductive tract of the male butterfly Atrophaneura alcinous Klug" Invertebrate Reproduction and Development. 34. 259-268 (1998)

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      1998 Annual Research Report
  • [Publications] Si,Y.and Okuno,M.: "Rebulation of microtubule sliding by a 36-kDa phosphoprotein in Hamster sperm flagella" Molecular Reproduction and Development. 52. 328-334 (1999)

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      1998 Annual Research Report
  • [Publications] Ishijima,S., Hara,M.and Okiyama M.: "Comparative studies on the spermatozoan motility of Japanese fshes." Bull.Ocean Res.Inst.Univ.Tokyo. 33. 139-152 (1998)

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      1998 Annual Research Report
  • [Publications] Hadjantonakis,A. et al.(Okaze,M.): "Non-invasive sexing of preimplantation stage mammalian embryos." Nat Genet. 19. 220-222 (1998)

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      1998 Annual Research Report
  • [Publications] Ikawa,M. et al.(Okabe,M.): "Green mice' and their potential usage in biological research." FEBS Lett. 430. 83-87 (1998)

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      1998 Annual Research Report
  • [Publications] Tsujimura,A. et al.(Okabe,M.): "Molecular cloning of a murine homologue of membarane cofactor protein (CD46) : preferential expression in testicular germ cells." Biochem.J.330. 163-168 (1998)

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      1998 Annual Research Report
  • [Publications] Yamagata,K. et al.(Okabe,M.): "Acrosin accelerates the dispersal of sperm acrosomal proteins during acrosome reaction." J.Biol.Chem.273. 10470-10474 (1998)

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      1998 Annual Research Report
  • [Publications] Kagami,O. et al.(Ogawa,K.): "A dynein light chain of sea urchin sperm flagella is a homolog of mouse T ctex L which is encoded by a gene of the t complex sterility locus" Gene. 211. 383-386 (1998)

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      1998 Annual Research Report
  • [Publications] Suarez,S.S. et al.: "Bull sperm binding to oviductal epithelium is mediated by a Ca2+- dependent lectin on sperm that recognizes Lewis-a grisaccharide." Biol.Reprod.59. 39-44 (1998)

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      1998 Annual Research Report
  • [Publications] Suarez,S.S. et al.: "Minireview. The Oviductal sperm reservoir in mammals : Mechanisms of Formation." Biol.Reprod.58. 1105-1107 (1998)

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      1998 Annual Research Report
  • [Publications] Ho,H.C., Suarez,S.S.: "Three-dimensional structure of the Golgi complex in mouse spermatids : a scanning electron microscope study." Proceedings of the International Meeting of Spermatology, Montreal, Canada, August 1998. Abs.2-43 (1998)

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  • [Publications] Imai,E. et al.(Okabe,M.): "Glowing podocytes in living mouse : transgenic mouse carrying a podocyte-specific promoter (In Process Citation)" Exp Nephorol. 7. 63-66 (1999)

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  • [Publications] Koga,M. et al.(Okabe,M.): "Expression of selenoprotein-P messenger ribonucleic acid in the rat testis." Biol.Rep.58. 261-265 (1998)

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  • [Publications] Matsuki,M. et al.(Okabe,M.): "Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase)" Proc Natl Acad Sci USA. 95. 1044-1049 (1998)

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  • [Publications] Mikata,S. et al.(Okabe,M.): "Revention of hyperacute rejection by phosphatidylinositol-anchored mini-complement receptor type 1." Transpl Immunol. 6. 107-110 (1998)

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  • [Publications] Okaze,M. et al.: "Green fluorescent protein and its usage in reproductive biology." Zygote. 6. 117-117 (1998)

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  • [Publications] Takahama,Y. et al.(Okabe,M.): "Functional competence of T cells in the absence glycosylphosphatidylinositol-anchored proteins caused by T cell-specific disruption of the Pig-a gene." Eur.J.Immunol.28. 2159-2166 (1998)

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  • [Publications] Revah I, et al.(Suarez,S.S.): "Changes in capacity of bull sperm to bind fucose." Biol Repro 58 (Suppl 1). Abs.147-147 (1998)

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  • [Publications] Maleszewski,M. et al.(Yanagimachi,R.): "Behavior of transgenic mouse spermatozoa with galline protamine." Biol.Reprod.58. 8-14 (1998)

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  • [Publications] Stewart-Savage,J. et al.: "Developmental basis of phenotypic variation in egg production in a colonial ascidian : primary oocyte production versus oocyte development." Biol.Bull.196. 63-69 (1999)

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      1998 Annual Research Report
  • [Publications] Stewart-Savage,J. et al.: "Suppression of motility in actively moving hamster sperm by hexose." Eighth International Symposium on Spermatology, August 1998, Montreal, Canada. (Poster presentation). (1998)

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  • [Publications] Si, Y.: "Temperature-dependent hyperactivated movement of hamster spermatozoa." Biology of Reproduction. 57. 1407-1412 (1997)

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  • [Publications] 石島 純夫: "精子運動のメカニズム" 日本受精着床学会誌. 14. 7-10 (1997)

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  • [Publications] Lefebvre, R.& Suarez, S.S.: "Bovine sperm binding to oviductal epithelium involves fucose recognition" Biology of Reproduction. 56. 1198-1204 (1997)

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  • [Publications] Dobrinski, I., Smith, T.T., Suarez, S.S., Ball, B.A.: "Membrane contact with oviductal epithelium modulates intracellular calcium concentratin of equine spermatozoa in vivo." BIology of Reproduction. 56. 861-869 (1997)

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      1997 Annual Research Report
  • [Publications] Usui, N., Ogura, A., Kimura, Y., Yanagimachi, R.: "Sperm nuclear envelope:Breakdown of intrinsic envelope and de novo formation in hamster oocytes and eggs." Zygote. 5. 35-46 (1997)

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      1997 Annual Research Report
  • [Publications] Yudin, A.I., Cherr, G.N., Vandevoort, C.A., Overstreet, J.W.: "Rearrangement of the PH-20 protein on the surface of macaque spermatozoa following exposure to anti-PH-20 antibodies or binding to zona pelucida." Molecular Reproduction and Development. in press. (1998)

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  • [Publications] Ogawa, K.& Mohri, H.: "Recent Advances in Marine Biotechnology" Oxford & IBH Publishing Co., 32 (1997)

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Published: 1997-03-31   Modified: 2016-04-21  

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