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The reaction mechanisms of the oxygenic photosynthesis. - Analyses based on the phylogeny of chloroplast proteins

Research Project

Project/Area Number 09044241
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionYAMAGUCHI UNIVERSITY

Principal Investigator

MIMURO Mamoru  Ymaguchi Univ, Dept.Biol.Prof., 理学部, 教授 (40142004)

Co-Investigator(Kenkyū-buntansha) TANAKA Ayumi  Hokkaido Univ.Low temp.Research Inst., Prof., 低温科学研究所, 教授 (10197402)
IKEUCHI Masahiko  Univ.Tokyo, Dept.Biol., Assoc.Prof., 大学院・総合文化研究科, 助教授 (20159601)
MATSUURA Katsumi  Tokyo Metropol.Univ.Dept.Biol.Assoc.Prof., 大学院・理学研究科, 助教授 (30181689)
PLUMLEY F.G.  アラスカ州立大学, 海洋学部, 助教授
BLANKENSHIP アールイー  アアリゾナ州立大学, 生化学部, 教授
GANTT E.  メリーランド州立大学, 微生物学部, 教授
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsPhotosynthesis / Systematics / Reaction mechanism / Evolution / 光合成生物の進化 / 細胞内共生 / 光化学系 / アンテナ色素蛋白質 / ストレス蛋白質 / 酸素発生型光合成 / 葉緑体タンパク質 / 光合成の反応機構 / シアノバクテリア
Research Abstract

Reaction mechanisms in photosynthesis will be well understood by introducing the idea of evolution, as shown by the continuity of reaction center complexes from photosynthetic bacteria to oxygenic photosynthetic organisms. There remain many large gaps in the reaction mechanisms in oxygenic photosynthesis, for example, an origin of oxygenic photosynthetic organisms, an origin of water oxidation machinery, evolution of Ch1 a/c algae by the secondary endosymbiosis, and so on. To approach to these large gaps, it is a good idea to compare reaction properties among several groups of oxygenic organisms. We attempted to understand the reaction mechanisms of several critical points by experimental approach and by group discussion.
(1) Localization of cytochrome c550, an essential protein for oxygen evolution in cyancibacteria and red algae, and its stoichiometry per reaction center II was investigated. In the red alga Porphyridium cruentum, its localization was not necessarily clear, because of … More its low content. On the other hand, in cyanobacteium Synechocystis sp. PCC6803, there are plural genes related to this cytochrome, however function of individual components were not necessarily clear.
(2) A group discussion was realized by a mini-symposium held in the University of Alaska, Fairbanks, in the summer of 1999. Based on the results obtained by the members of this research, we commonly understood the following points. (I) In the course of evolution of photosynthetic bacteria, Chloroflexus aurantiacus, which was postulated to be the oldest ancestor of bacteria, might not be the case based on the gene structure of pigment biosynthetic pathways. (ii) Oxygenic photosynthetic prokaryote probably acquired several genes for pigment synthesis in the early stage of evolution. (iii) A gene structure of dinoflagellates was unique ; one-gene is composed of one-circle. This is completely unexpected property, but its biological significance was not yet clear.
Based on the above results and discussion, all the members agreed to continue this kind of co-operative research for the next chance. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (56 results)

All Other

All Publications (56 results)

  • [Publications] Tomitani, A., Tanaka,A.et al: "Chlorophyll b and phycobilins in the common ancestor of cyanobacteria and chloroplasts"Nature. 400. 159-162 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka, A. et al: "Isolation of chlorophyll a oxygenase which is involved in chlorophyll a formation from chlorophyll a"Proc. Natl. Acad. Sci, USA. 95. 12719-12723 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masuda, S. Matsuura, K. et al: "A new cytochrome subunit bound to the photosynthetic reaction center in the purple bacterium, Rhodovulum sulfidophilum"J. Biol. Chem.. 16. 10795-10801 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagashima, K.V.P., Matsuura, K. et al: "Horizontal transfer of genes coding for the photosynthetic reaction centers of purple bacteria"J. Mol. Evol.. 45. 131-136 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hihara. Y., Sonoike, K. and Ikeuchi, M.: "A novel gene, pmgA, specifically regulates photosystem stoichiometry in the cyanobacterium Synechocystis sp. PCC6803 in response to high light"Plant Physiol.. 73. 221-229 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shen, J.-R., Ikeuchi, M. et al: "Analysis of the psbU gene encoding the 12-kDa extrinsic protein of Photosystem II and studies on its role by deletion mutagenesis in Synechocystis sp. PCC6803"J. Biol. Chem.. 272. 17821-17826 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro, M. et al: "Magnetic circular dichroism(MCD) properties of reaction center complexes isolated from the Zn-Bacteriochlorophyll a containing purple vacterium, Acidiphilium rubrum"Biochemistry. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro et al: "Fluorescence properties of Chlorophyll d-dominating prokaryotic alga, Acaryochloris marina : Studies by means of the time-resolved spectroscopy"Biochim. Biophys. Acta.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro, M. et al: "Concepts in Photobiology : Photosynthesis and Photomorphogenesis"Kluwer Academic Pub.. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 三室守: "藻類の多様性と系統"裳華房. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro, M., S. Akimoto, S. Takaichi and I. Yamazaki: "Effect of molecular structure and solvents on the excited state dynamics of the S2 state of carotenoids analyzed by means of the femtosecond up-conversion technique"J.Am.Chem.Soc.Communication. 119. 1452-1453 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oba, T., M. Mimuro, Z.Y. Wang, T, Nozawa, S. Yoshida and T. Watanabe: "Spectral characteristics and collodal properties of chlorophyll a' in aqueous methanol"J.Phys.Chem.. 101B. 3261-3268 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kikuchi, H., T. Sugimoto, and M. Mimuro: "An electronic state of the chromophore, phycocyanobilin, and its interaction with the protein moiety in C-phycocyanin : protonation of the chromophore"Chem.Phys.Lett.. 274. 460-465 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomo, T., M. Mimuro, M. Iwaki, M. Kobayashi, S. Itoh and K. Satoh: "Topology of pigments in the isolated PS II reaction center studied by selective extraction"Biochem.Biophys.Acta. 1321. 21-30 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamano, Y., M. Mimuro, and M. Ito: "Carotenoids and related polyenes. Part 4. Synthesis of carotenoid analogues containinga conjugated carbonyl group and their florescence properties"Chem.Soc.Perkin Tran.1. 1997(18). 2713-2724 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro, M., N. Tamai, A. Murakami, M. Watanabe, M. Erata, M. Watanabe, M. Tokutomi and I. Yamazaki: "Multiple pathways of the excitation energy flow in the phtosynthetic pigment system of a cryptophyta, Cryoptomonas sp.(CR-1)"Psycological Res.. 46. 155-164 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takaichi, S. and M. Mimuro: "Distribution and geometrical isomerism of neoxanthin in oxygenic phototrophs : 9'-cis, a sole molecular form"Plant & Cell Physiol.. 39. 968-977 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oba, T., H. Furukawa, Z-Y. Wang, T. Nozawa, M. Mimuro, H. Tamiaki and T. Watanabe: "Supramolecular structure of the chlorophyll a' aggregate, and the origin of diastreoselective separation of chlorophyll a and a'"J.Phys.Chem.B.. 102. 7882-7889 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Papageorgiou, G. C., Govindjee, R. Govindjee, M. Mimuro, K. Stamatakis, A. Alygizaki-Zorba and N. Murata: "Membrane lipid unsaturation modulates light-induced and osmotically-induced shift in chlorophyll a fluorescence of Synechocystis sp.PCC 6803"Photosynthe.Res.. 59. 125-136 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro, M., Akimoto, S., Yamazaki, I., Miyashita, M. and Miyachi, S.: "Fluorescence properties of Chlorophyll d-dominating prokaryotic alga, Acaryochloris marina : Studies by means of the time-resolved spectroscopy"Biochim.Biophys.Acta. 1412. 37-46 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Akimoto, I. Yamazaki, S. Takaichi and M. Mimuro: "Excitation relaxation of carotenoids within the S2 state probed by the femtosecond fluorescence up-conversion method"Chem.Phys.Lett.. 313. 63-68 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishii, T., K. Uehara, Y. Ozaki, and M. Mimuro: "The effects of pH and ionic strength on the aggregation of bacteriochlorophyll c in aqueous organic media : possibility of two kinds of aggregation forms"Photochem.Photobiol.. 70. 760-765 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro, M., K. Hirayama, K. Uezono, H. Miyashita, and S. Miyachi: "Up-hill energy transfer in a chlorophyll d-dominating oxygenic photosynthetic prokaryote, Acaryochloris marina"Biochim.Biophys.Acta. 1456. 27-34 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iida, K., N. Ohya, A. Kashiwada, M. Mimuro and M. Nango: "Characterization of the light-harvesting polypeptide/bacteriochlorophyll a complex isloated from photosynthetic bacteria by linear dichroism spectra"Bull.Chem.Soc.Japan. 73. 221-229 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Akimoto, I. Yamazaki, S. Takaichi and M. Mimuro: "Excitation relaxation dynamics of linear carotenoids"J.Luminescence. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mimuro, M., M. Kobayashi, K. Shimada, K. Uezono, and T. Nozawa: "Magnetic circular dichroism (MCD) properties of reaction center complexes isolated from the Zn-Bacteriochlorophyll a containing purple bacterium, Acidiphilium rubrum"Biochemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Papageorgiou,G.C.et al.: "Membrane lipid unsaturation modulates light-induced and osmotically-induced shift in chlorophyll a fluorescence of synechocystis sp.PCC 6803."Photosynthe.Research. 59. 125-136 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mimuro,M.et al.: "Fluorescence properties of Chlorophyll d-dominating prokaryotic alga,Acaryochloris marina:Studies by means of the time-resolved spectroscopy."Biochimica et Biophysica Acta. 1412. 37-46 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akimoto,S.et al.: "Excitation relaxation of carotenoids within the S2 state probed by the femtosecond fluorescence up-conversion method."Chemical Physics Letters. 313. 63-68 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishii,T.et al.: "The effects of pH and ionic strength on the aggregation of bacteriochlorophyll c in aqueous organic media:possibility of two kinds of aggregation forms,"Photochemistry and Photobiology. 70. 760-765 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iida,K.et al.: "Characterization of the light-harvesting polypeptide/bacteriochlorophyll a complex isloated from photosynthetic bacteria by linear dichroism spectra."Bull.Chem.Soc.Japan. 73. 221-229 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mimuro,M.et al.: "Up-hill energy transfer in a chlorophyll d-dominating oxygenic photosynthetic prokaryote,Acaryochloris marina."Biochimica et Biophysica Acta. 1456. 27-34 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mimuro,M.et al.: "Magnetic circular dichroism(MCD)properties of reaction center complexes isolated from the Zn-Bacteriochlorophyll a containing purple bacterium,Acidiphilium rubrum."Biochemistry. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Akimoto,S.et al.: "Excitation relaxation dynamics of linear carotenoids"J.Luminescence. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 山崎 巌 他: "光受容蛋白質における超高速光化学反応のレーザー分光による計測"レーザー生物・医学応用. RTM-99-31. 29-34 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mimuro,M.et al.: "Concepts in Photobiology:Photosynthesis and Photomorphogenesis."Kluwer Academic Pub.. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三室 守: "藻類の多様性と系統"裳華房. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三室 守: "光がもたらす生命と地球の共進化:エネルギーと食糧と地球環境に対するグローバルな視点"中部経済新聞社. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三室 守: "電子と生命-新しいバイオエナジェッティックスの展開"共立出版(印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Mimuro,M.,et al.: "Multiple pathways of the excitation energy flow in the phtosynthetic pigment system of a cryptophyta,Cryoptomonas sp.(CR-1)." Phycological Research. 45. 155-164 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takaichi,S.and M.Mimuro: "Distribution and geometrical isomerism of neoxanthin in oxygenic phototrophs : 9'-cis,a sole molecular form." Plant & Cell Physiology. 39. 968-977 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Oba,T.,et al.: "Supramolecular structure of the chlorophyll a'aggregate, and the origin of diastreoseletive separation of chlorophyll a and a'." Journal of Physical Chemistry B. 102. 7882-7889 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Papageorgiou,G.C et al.: "Membrane lipid unsaturation modulates light-induced and osmotically-induced shift in chlorophyll a fluorescence of Synechocystis sp.PCC 6803." Photosynthesis Research. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Mimuro,M.,et al.: "Fluorescence properties of Chlorophyll d-dominating prokaryotic alga,Acaryochioris marina : Studies by means of the time-resoived spectroscopy." Biochimica et Biophysica Acta. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 三室 守,秋本誠志: "カロテノイドの励起状態ダイナミクス-生体系における役割の決定因子" 光化学. 29. 6-13 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Mimuro et al.: "Relaxation processes from excited satates and physiological function of carotenoids : Comparison of two molecular forms of neoxanthin" Photomedicine and Photobiology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Mimuro et al.: "Fluorescence properties of chlorophyll d-dominating prokaryotic alga,Acaryochloris marina." Proceedings of the Intenationa Congres on Photosynthesis. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Kikuchi et al.: "Functioanl meaning of the symmetry structure in phycobiliproteins aggregates." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Takaichi and M.Mimuro: "Distribution of 9'-cis neoxanthin in oxygenic phototrophs : A systematic study." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] G.C.Papageorgiou et al.: "Temperature and Iipid unsaturation effects on plasma membranes (PM) and thylakoid membranes (TH) of Synechocyststis sp,PCC6803." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Mimuro,M.,H.Kikuchi and A.Murakami: "Photosynthesis and Plant Photobiology" Academic Press, London (in press),

    • Related Report
      1998 Annual Research Report
  • [Publications] 三室 守: "多様性シリーズ 藻類の多様性" 裳華房(in press),

    • Related Report
      1998 Annual Research Report
  • [Publications] 三室 守: "光がもたらす生命と地球の共進化:エネルギーと食糧と地球環境に対するグローバルな視点" 弘文社出版(印刷中),

    • Related Report
      1998 Annual Research Report
  • [Publications] 三室 守: "シアノバクテリアの集光性超分子会合体、フィコビリソーム" 蛋白質・核酸・酵素. 42. 2613-2625 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 三室 守: "光合成タンパク質の系統性と進化" 化学と生物. 35. 822-824 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 三室 守: "多様性シリーズ" 裳華房(印刷中),

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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