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Mechanisms of absorption, transport, and storage of vitamin A-by using rats and polar animals-

Research Project

Project/Area Number 09044251
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionAkita University

Principal Investigator

SENOO Haruki  Akita University, Department of Anatomy, Professor, 医学部, 教授 (90171355)

Co-Investigator(Kenkyū-buntansha) IMAI Katsuyuki  Akita University, Department of Anatomy, Research Associate, 医学部, 助手 (80006741)
SATO Mitsuru  Akita University, Department of Anatomy, Research Associate, 医学部, 助手 (60226008)
BERG Trond  University of Oslo, Department of Cell Biology, Professor, 医学部, 教授
BLOMHOFF Rune  University of Oslo, Institute for Nutrion Research, Professor, 医学部, 教授
NORUM Kaare R.  University of Oslo, Institute for Nutrion Research, Professor, 医学部, 教授
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsvitamin A / Arctic animals / hepatic stellate cells / retinyl ester / Svalbard archipelago / hypervitaminosis A / food web / hepatic fibrosis / レエチニルエステル / スバルバ-ル群島 / 自家蛍光 / 塩化金法
Research Abstract

Two polar bears, 8 Arctic foxes, 6 bearded seals, 22 glaucous gulls, 5 fulmars, 4 Bruenich's guillemots, 6 ringed seals, 5 hooded seals, 6 puffins, and 7 Svalbard reindeers were caught in the Svalbard archipelago (situated at lat. 80 N). Fresh organs, namely, the liver, kidney, spleen, lung, and jejunum were investigated with gold chloride staining, fluorescence microscopy for autofluorescence of vitamin A, hematoxylin and eosin staining, azan staining, Ishii-Ishii's silver impregnation, transmission electron microscopy and high-performance liquid chromatography.
The mammals and birds stored a large amount of vitamin A in the liver [23,300 (polar bear, n=2), 18,439*2,314 (Arctic fox, n=8), 5956*5857 (bearded seal, n=6), 1,551*2,486 (ringed seal, n=6), 921*264 (Svalbard reindeer, n=7), 4,710*3,164 (glaucous gull, n=22), 1,589*225 (Bruenich's guiHemot, n=4), and 1183*589 (puffin, n=6) nmole retinyl ester/g wet tissue]. Very strong autofluorescence of vitamin A emanated from the non-parenchymal cells in the liver. These cells showed positive reaction with gold chloride and stained black. Electronmicroscopically these cells existed in the perisinusoidal space of Disse and contained many lipid droplets within the cytoplasm. These anamals showed no hepatic fibrosis though they are in naturally occurred hypervitaminosis A.
These findings suggest that these vitamin A-storihg cells are hepatic stellate cells and the Arctic animals that exist in a higher position of the food web in the Arctic area have well-developed vitamin A-storing mechanisms in the hepatic stellate cells.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Senoo H,et al.: "Hepatic stellate cells-from viewpoint of retinoid handling and functions of extracellular matrix" Acta Anat Nippon. 72. 79-94 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagy NE,et al.: "Storage of vitamin A in extrhepatic stellate cells in normal rats" J Lipid Res. 38. 645-658 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Senoo H, et al.: "Molecular mechanisms in the reversible regulation of morphology, proliferation, and collagen metabolism in hepatic stellate-cells by the three-dimensional structure of the ECM" J Gastroent Hepatol. 13. S19-32 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima N,et al.: "Stellate cells (vitamin A-storing cells) change their cytoskeleton and signal transduction system according to extracellular matrix" Histochem Cell Biol. 110. 121-128 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Senoo H, et al.: "Vitamin A-storing cells in mammals and birds in Arctic area-a study in the Svalbard archipelago-" Cells Hepatic Sinusoid. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matano Y,et al.: "Hepatic stellate cells and extrahepatic stellate cells (extrahepatic vitamin A-storing cells)" Cells Hepatic Sinusoid. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 妹尾春樹、他: "細胞外マトリックス研究法" コラーゲン技術研修会, 25 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Senoo H,et al.: "Hepatic stellate cells-from the viewpoint of retinoid handling and functions of extracellular matrix." Acta Anat Nippon.72. 79-94 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagy NE,et al.: "Storage of vitamin A in extrahepatic stellate cells in normal rats." J Lpid Res.38. 645-658 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Senoo H,et al.: "Molecular mechanisms in the reversible regulation of morphology, proliferation, and collagen metabolism in hepatic stellate cells by the three-dimensional structure of the extracellular matrix." J Gastroent Hepatol.13. S19-32 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima N,et al.: "Stellate cells (vitamin A-storing cells) change their cytoskeleton and signal transduction system according to extracellular matrix." Histochem Cell Biol.110. 121-128 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Senoo H,et al.: "Vitamin A-storing cells in ammmals and birds in Arctic area-a study in the Svalbard archipelago-." Cells Hepatic Sinusoid. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matano Y,et al.: "Hepatic stellate cells and extrahepatic stellate cells (extrahepatic vitamin A-storing cells)" Cells Hepatic Sinusoid. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Senoo H,et al.: "Research Methods for Extracellular Matrix" Collagen Research Study and Training Society. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Senoo H,et al.: "Hepatic stellate cells-from the viewpoint of retinoid handling and functions of extracellular matrix-" Acta Anat Nippon. 72. 79-94 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nagy NE,et al.: "Storage of vitamin A in extrahepatic stellate cells in normal rats" J Lipid Res. 38. 645-658 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Senoo H,et al.: "Molecular mechanisms in the reversible regulation of morphology,proliferation,and collagen metabolism in hepatic stellate cells by the three-dimensional structure of ECM" J Gastroent Hepatol. 13. S19-32 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kojima N,et al.: "Stellate cells(vitamin A-storing cells)change their cytoskeleton and signal transduction system according to extracellular matrix" Histochem Cell Biol. 110. 121-128 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Senoo H,et al.: "Vitamin A-stroing cells in mammals and birds in Arctic area- a study in the Svalbard archipelago-" Cells Hepatic Sinusoid. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Matano Y,et al.: "Hepatic stellate cells and extrahepatic stellate cells(extrahepatic vitamin A-storing cells)" Cells Hepatic Sinusoid. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 妹尾春樹、他: "細胞外マトリックス研究法" コラーゲン技術研修会, 25 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Senoo H.: "Hepatic stellate cells From the viewpoint of retinoid handling and function of extracellular matrix-" Acta Anat. Nippon.72. 79-94 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagy N.: "Storage of vitamin A in extrahepatic stellate cells in normal rats" J. Lipid Res.38. 645-658 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Senoo H.: "Vitamin A-storing cells in animals in the extreme environment-a study in the Svalbard Archipelago" Cell Strruct. Func.22. 748 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Senoo H.: "Vitamin A-storing system in the Arctic animals" Acta Anat. Nippon.72. 301 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Imai K.: "Morphology of sites of adhesion between hepatic stellate cells(vitamin A-storing cells)and a three-dimensional extracellular matrix" Anat. Rec.250(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Senoo H.: "Molecular mechanisms in the reversible regulation of morphology, proliferation,and collagen metabolism in hepatic stellate cells by three-dimensional structure of extracellular matrix" J. Gastroenterol. Hepatol.(in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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