| Project/Area Number |
09044253
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Pathological medical chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
BANNAI Shiro Institute of Basic Medical Sciences, University of Tsukuba, Professor, 基礎医学系, 教授 (70019579)
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| Co-Investigator(Kenkyū-buntansha) |
マン ジョバンニ・E キングスカレッジ, ロンドン, 教授
SATO Hideyo Institute of Basic Medical Sciences, University of Tsukuba, Lecturer, 基礎医学系, 講師 (60235380)
ISHII Tetsuo Institute of Basic Medical Sciences, University of Tsukuba, Associate Professor, 基礎医学系, 助教授 (20111370)
MANN Giovanni E. King's College London, Professor
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| Project Period (FY) |
1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | oxidative stress / carbon monoxide / nitrogen monoxide / vascular cells / macrophages / low-density lipoprotein |
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| Research Abstract |
Carbon monoxide (CO) and nitric monoxide (NO) play an important role in the physiological regulation of vascular tone and pathogenesis of atherosclerosis. In the present study we have obtained the following results.
1. In human vascular smooth muscle cells, the activity of heme oxygenase, which generates CO,was induced by oxidized LDL and oxidative stress in a time-and dose-dependent manner.
2. In human endothelial cells, the transport activity of arginine was stimulated and the production of NO increased by culturing the cells in higher concentration of glucose.
3. In mouse macrophages, heme oxygenase was induced by oxidized LDL.However, the transport activity of arginine remained unchanged and production of NO was undetectable by oxidized LDL.
From these results, it is suggested that under oxidative stress conditions heme oxygenase induced by oxidative stress increases the production of CO whereas NO is not generated. It is likely that the regulation of CO production is different from that of NO production. CO may function as the modulator of vascular tone under the oxidative stress conditions which are different from those where NO functions. Manuscripts containing these results are in preparation for papers.
In 1998 UK98 Festival supported by the governments of UK and Japan will be held in Japan. In the course of the present joint research, we agreed as one of the events of UK98 Festival to hold a symposium entitled "Regulation of antioxidant system in oxidative stress" at University of Tsukuba, November l998. The British Council has offered a financial support for this symposium.
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