| Project/Area Number |
09044255
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research . |
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| Research Field |
Molecular biology
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| Research Institution | Tohoku University (1998)
University of Tsukuba (1997) |
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Principal Investigator |
IGARASHI Kazuhiko TOHOKU UNIV.SCHOOL OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (00250738)
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| Co-Investigator(Kenkyū-buntansha) |
PATIENT Roger KING'S COLLEGE,LONDON,PROFESSOR, ランドール研究所, 教授
ENGEL J.Douglas NORTHWESTERN UNIV.PROFESSOR, 教授
YAMAMOTO Masayuki TSUKUBA UNIV.INSTITUTE OF BASIC MEDICAL SCIENCES,PROFESSOR, 基礎医学系, 教授 (50166823)
プーシエント ロジャー キングスカレッジ, ランドール研究所, 教授
エンゲル ダグラス ノースウェスタン大学, 生化学部門, 教授
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | NF-E2 / GATA / Bach / KNOCKOUT MOUSE / 血液細胞 / 赤血球 / 転写因子 / 細胞分化 |
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| Research Abstract |
Hematopoiesis is a good model system for analyzing regulation of cell differentiation. Using this system, we tried to identify transcription factors that regulate cell differentiation. We analyzed function of NF-E2 and related factors, and accumulated novel as well as unexpected results. These findings should lead to understanding of molecular and genetic regulation of hematopoiesis.
(1) To identify novel NF-E2-related transcription factors, we carried out further homology screening. As a result, we succeeded in identifying sixth member of the NF-E2-related factors which we named Nrf3. Its expression profile suggested that Nrf3 may regulate differentiation of hematopoietic cell differentiation. We are now carrying out gene disruption experiment using mice system, together with Prof.Engel.
(2) By making nrf2-knockout mice and analyzing its phenotypic changes, we revealed that Nrf2 is a key essentail regulator of phase II de-toxifying system expression.
(3) The BTB domain of Bach1 and Bach2 appears to be involved in regulation of chromatin structure. Using atomic force microscopy, we revealed that Bach1, and possible Bach2, function as a novel type of architectural transcription factor that mediate DNA loop formation. As such, Bach1 may be involved in regulation of the globin gene locus control region.
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