| Project/Area Number |
09044257
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Human genetics
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| Research Institution | GUNMA UNIVERSITY |
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Principal Investigator |
TAKEDA Jun INSTITUTE FOR MOLECULAR AND CELLULAR REGULATION GUNMA UNIVERSITY PROFESSOR, 生体調節研究所, 教授 (40270855)
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| Co-Investigator(Kenkyū-buntansha) |
高橋 健一郎 群馬大学, 医学部, 日本学術振興会特別研
LEBEAU Michelle M. DEPARTMENT OF MEDICINE,UNIVERSITY OF CHICAGO,ASSOCIATE PROFESSOR, 血液学/腫瘍学部門, 準教授
BELL Greame シカゴ大学, ハワードヒューズ医学研究所, 教授
IZUMI Tetsurou INSTITUTE FOR MOLECULAR AND CELLULAR REGULATION GUNMA UNIVERSITY ASSOCIATE PROFE, 生体調節研究所, 助教授 (00212952)
TAKEUCHI Toshiyuki INSTITUTE FOR MOLECULAR AND CELLULAR REGULATION GUNMA UNIVERSITY PROFESSOR, 生体調節研究所, 教授 (00109977)
BELL Graeme I. HOWARD HUGHES MEDICAL INSTITUTE,UNIVERSITY OF CHICAGO,PROFESSOR
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| Project Period (FY) |
1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | GENOME / DATABASE / POSITIONAL CLONING / GENE EXPRESSION / CHROMOSOMAL MAPPING |
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| Research Abstract |
Diabetes mellitus is characterized by elevated plasma glucose levels due to an absolute or relative deficiency of insulin. The genetic linkage studies looking for diabetes susceptibility genes are well underway in many labs, but this "positional cloning" is still a laborious work. Because of the central role of the pancreatic islets in the regulation of glucose homeostasis, we focused on this tissue as a primary site of expression of the major diabetogenic genes and created a database of genes expressed in this tissue as expressed sequence tags (ESTs). The analysis of 6,055 ESTs by database searches idicated that approximately 50% of the cDNAs so far isolated represented unknown human genes. Of these, 2,731 and 402 ESTs shoewed exact match with and significant similarty to known genes, respectively. 2,390 ESTs showed homology with other ESTs. 408 ESTs had no database match. Approximately half of these genes have been assigned to the chromosome by dbSTS search, RH mapping and/or FISH.The characterization of the tissue distribution and chromosomal localization of novel ESTs will facilitate the "positional candidate" approach. The pancreatic islet ESTs obtained in this project will provide a unique key source of new candidate genes for genetic studies of diabetes.
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