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Novel calcium signaling pathways in cell cycle regulations

Research Project

Project/Area Number 09044282
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field General pharmacology
Research InstitutionNagoya University

Principal Investigator

NIKI Ichiro (1998)  School of Medicine, Associate Professor, 医学部, 助教授 (10262908)

日高 弘義 (1997)  名古屋大学, 医学部, 教授 (80100171)

Co-Investigator(Kenkyū-buntansha) MEIJER Laurent  Center National de la Recherche Scientifique, Station Biologique, Professor, 教授
NAITO Yasuhito  School of Medicine, Research Associate, 医学部, 助手 (80303618)
WATANABE Yasuo  School of Medicine, Assistant Professor, 医学部, 講師 (10273228)
LAURENT Meij  フランス海洋生物学研究所, 教授
仁木 一郎  名古屋大学, 医学部, 助教授 (10262908)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsprotein kinase inhibitor / cell cycle / cdc2 kinase / isoquinolinesulphonamide compounds / isopurine compounds / プロテインキナーゼ / カルシウムシグナリング / 分子プローブ
Research Abstract

During our two-year collaboration, we have extensively discussed the possibility to create novel and selective inhibitors of various protein kinases. A part of the result was presented by L.M.in a symposium of the 7lth Annual meeting of Japanese Biochemical Society held in Nagoya last October. Our mutual interest was whether isoquinoline-sulphonamide derivatives exhibit any inhibition on the activities of cell-cycle-related kinases, because some of the isoquinoline-sulphonamide compounds such as H-7 could be regarded as 'general kinase inhibitors'. Therefore, we attempted to look for a cdc2-kinase inhibitor from a variety of isoquinoline compounds. Among several compounds surveyed, H-1152 and its derivatives were, indeed, found to inhibit cdc2 kinase at low muM concentrations. However, these inhibitors were neither selective nor potent as isopurine compounds as olomoucine or roscovitine developed by L.M.This suggests to us that their relevant mechanisms of inhibition may be distinct, although both isoquinoline and isopurine compounds exhibited ATP-competitive inhibition. Another interest was whether potency of the inhibition by these ATP-competitive compounds may simply reflect a ATP-requirement of each protein kinase, because this possibility was implicated in inhibition of Rho-kinase by isoquinoline-suiphonamide inhibitors (H-7 and HA-1077). Eventually it was proved that such was, however, not the case after evaluation of the effects of these compounds against other protein kinases with different ATP-requirements. This is a crucial point to design novel protein kinase inhibitors and their application to a clinical use.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (78 results)

All Other

All Publications (78 results)

  • [Publications] Iida Y.: "Myosin light chain phosphorylation controls insulin secretion at a proximal step in the secretory cascade." Am.J.Physiol.(Endocrinol.Metab.36). 273. E789-E782 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kabir N.: "Immunocytochemical detection and spatial distribution of myosin light-chain kinase in preimplantation mouse embryos." J.Exp.Zool.278. 147-155 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe Y.: "Identification of a specific amino acid cluster in the calmodulin-binding domain of the neuronal nitric oxide synthase" FEBS Lett.403. 75-78 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naito Y.: "Isoform-specific activation and structural diversity of CaM kinase I" J.Biol.Chem.272. 32704-32708 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokokura H.: "Isolation and comparison of rat cDNAs encoding Ca^<2+>/calmodulin-dependent protein kinase I isoforms." Biochim.Biophys.Acta (Short sequence paper). 1338. 8-12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hidaka H.: "Cascade activation of the calmodulin kinase family." in Signal Transduction in Health and Disease, edited by J.Corbin, Raven Press, New York, U.S.A.(Proceedings of the 9th International Conference on Second Messengers and Phosphoproteins, Oct.27-Nov.1, 1995, Nashville, U.S.A.). 151-157 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 渡邊泰男: "NOSの分子生物学的解析による構造機能活性相関ならびに活性化メカニズムの検討" 日本薬理学雑誌(Folia Pharmacol.Jpn.). 110. 69-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Schutte B.: "The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics." Exp Cell Res.236・1. 4-15 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] De Azevedo WF.: "Inhibition of cyclin-dependent kinases by purine analogues : crystal structure of human cdk2 complexed with roscovitine." Eur.J.Biochem.243・1-2. 518-526 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Meijer L.: "Biochemical and cellular effects of roscovitine,a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5." Eur.J.Biochem.243・1-2. 527-536 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] HavlIcek L.: "Cytokinin-derived cyclin-dependent kinase inhibitors : synthesis and cdc2 inhibitory activity of olomoucine and related compounds." J.Med.Chem.40・4. 408-412 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Pippin JW.: "Direct in vivo inhibition of the nuclear cell cycle cascade in experimental mesangial proliferative glomerulonephritis with Roscovitine, a novel cyclin-dependent kinase antagonist." J.Clin.Invest.100・10. 2512-2520 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Krucher NA.: "The cyclin-dependent kinase (cdk) inhibitors, olomoucine and roscovitine, alter the expression of a moliuscan circadian pacemaker." Cell Mol.Neurobiol.17・5. 495-507 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Meijer L.: "Chemical inhibitors of cyclin-dependent kinases." Methods Enzymol.283. 113-128 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Planchais S.: "Roscovitine, a novel cyclin-dependent kinase inhibitor, characterizes restriction point and G2/M transition in tobacco BY-2 cell suspension." Plant J.12・1. 191-202 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe H.: "An essential role of myosin light-chain kinase in the regulation of agonist-and fluid-flow- stimulated Ca^<2+> influx in endothelial cells." FASEB J.12. 341-348 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Niwa T.: "Acetylcholine activates intracellular movement of insulin granules in the pancreatic β-cells via IP3-dependent mobilization of intracellular Ca^<2+>." Diabetes. 47. 1699-1706 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 仁木一郎: "インスリン顆粒運動の制御と細胞内のCa^<2+>の分業仮説." Peptide Hormones in Pancreas. 18. 112-116 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe Y.: "Neuronal nitric oxide synthase-membrane phospholipid interaction" Arch.Biochem.Biophys.358. 68-73 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe Y.: "Inter-isoformal regulation of nitric oxide synthase through heteromeric dimerization" Biochim.Biophys.Acta. 1388. 199-208 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishio M.: "HMN-1180, a small molecule inhibitor of neuronal nitric oxide synthase" J.Pharmacol.Exp.Ther.287. 1063-1067 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakaguchi H.: "Unique inhibitory action of the synthetic compound 2-[N-(2-aminoethyl)-N-(5-isoquinolinesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine (CKA-1306) against calcium/calmodulin-dependent protein kinase I" Biochem.Pharmacol.56. 329-334 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gray NS.: "Exploiting chemical libraries, structure and genomics in the search for kinase inhibitors." Science. 281・5376. 533-538 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Niki I.: "Intracellular Ca^<2+> receptors in the pancreatic β-cell." Molec.Cell Biochem.in print. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Niki I.: "Ca^<2+> signalling and the insulin secretory cascade in pancreaticbeta-cell." Jap.J.Pharmacol.in print. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ono-Saito N.: "H-series protein kinase inhibitors and a case of a H-series inhibitor for clinical application." Pharmacol.Therap.in print. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 丹羽多恵: "グルコースによるインスリン分泌顆粒運動の活性化機構." Peptide Hormones in Pancreas. in print. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iida Y., Senda T., Matsukawa Y., Onoda K,Miyazaki J-1., Sakaguchi H., Nimura Y., Hidaka H., and Niki I.: "Myosin light chain phosphorylation controls insulin secretion at a proximal step in the secretory cascade." Am.J.Physiol.273 (Endocrinol.Metab.36). E782-E789 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kabir N., Yamamura H., Niki I., Iida Y., Uzzaman M., Sarkerz D., Hayasaka S., Takagishi Y., Inouye M., and Hidaka H.: "Immunocytochemical detection and spatial distribution of myosin light-chain kinasesin preimplantation mouse embryos." J.Exp.Zool.278. 147-155 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe Y., Hu Yu, and Hidaka H.: "Identification of a specific amino acid cluster in the calmodulin-binding domain of the neuronal nitric oxide synthase" FEBS Lett.403. 75-78 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naito Y., Watanabe Y., Yokokura H., Sugita R., Nishio M., and Hidaka H.: "Isoform-specific activation and structural diversity of CaM kinase I" J.Biol.Chem.272. 32704-32708 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokokura H., Terada O., Naito Y., and Hidaka H.: "Isolation and comparison of rat cDNAs encoding Ca^<2+>/calmodulin-dependent protein kinase I isoforms. (Short sequence paper)" Biochim.Biophys.Acta. 1338. 8-12 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hidaka H., Terada O., Naito Y., Sugita R., and Yokokura H.: "Cascade activation of the calmodulin kinase family." Signal Transduction in Health and Disease, edited by J.Corbin, Raven Press, New York, U.S.A.Proceedings of the 9th International Conference on Second Messengers and Phosphoproteins, Oct.27-Nov.1,1995, Nashville, U.S.A.151-157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Schutte B., Nieland L., van Engeland M., Henfling ME., Meijer L., and Ramaekers FC.: "The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics." Exp.Cell Res.236 (1). 4-15 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] De Azevedo WF., Leclerc S., Meijer L., Havlicek L., Strnad M., and Kim SH.: "Inhibition of cyclin-dependent kinases by purine analogues : crystal structure of human cdk2 complexed with roscovitine." Eur.J.Biochem.243 (1-2). 518-526 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Meijer L., Borgne A., Mulner O., Chong JP., Blow JJ., Inagaki N., Inagaki M., Delcros JG., and Moulinoux JP.: "Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5." Eur.J.Biochem.243 (1-2). 527-536 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] HavlIcek L., Hanus J., Vesel J., Leclerc S., Meijer L., Shaw G., and Strnad M.: "Cytokinin-derived cyclin-dependent kinase inhibitors : synthesis and cdc2 inhibitory activity of olomoucine and related compounds." J.Med.Chem.40 (4). 408-412 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Pippin JW., Qu Q., Meijer L., and Shankland SJ.: "Direct in vivo inhibition of the nuclear cell cycle cascade in experimental mesangial proliferative glomerulonephritis with Roscovitine, a novel cyclin-dependent kinase antagonist." J.Clin.Invest.100 (10). 2512-2520 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Krucher NA., Meijer L., and Roberts MH.: "The cyclin-dependent kinase (cdk) inhibitors, olomoucine and roscovitine, alter the expression of a molluscan circadian pacemaker." Cell Mol.Neurobiol.17 (5). 495-507 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Meijer L.and Kim SH.: "Chemical inhibitors of cyclin-dependent kinases." Methods Enzymol.283. 113-128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Planchais S., Glab N., TrEhin C., Perennes C., Bureau JM., Meijer L., and Bergounioux C.: "Roscovitine, a novel cyclin-dependent kinae inhibitor, characterizes restriction point and G2/M transition in tobacco BY-2 cell suspension." Plant J.12 (1). 191-202 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe H., Takahashi R., Zhang X-X., Goto Y., Hayashi H., Ando J., Isshiki M., Seto M., Hidaka H., Niki I., and Ohno R.: "An essential role of myosin light-chain kinase in the regulation of agonist-and fluid-flow-stimulated Ca^<2+> influx in endothelial cells." FASEB J.12. 341-348 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Niki I.and Hidaka H.: "Intracellular Ca^<2+> receptors in the pancreatic beta-cell." Molec.Cell Biochem.(in print). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Niwa T., Matsukawa Y., Senda T., Nimura Y., Hidaka H., and Niki I.: "Acetylcholine activates intracellular movement of insulin granules in the pancreatic beta-cells via IP3-dependent mobilization of intracellular Ca^<2+>." Diabetes. 47. 1699-1706 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe Y., Nishino M., Hamaji S., Hayashi Y., Hu Y., and Hidaka H.: "Neuronal nitric oxide synthase-membrane phospholipid interaction" Arch.Biochem.Biophys.358. 68-73 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe Y., Nishino M., Hamaji S., and Hidaka H.: "Inter-isoformal regulation of nitric oxide synthase through heteromeric dimerization" Biochim.Biophys.Acta. 1388. 199-208 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishio M., Watanabe Y., and Hidaka H.: "HMN-1180, a small molecule inhibitor of neuronal nitric oxide synthase" J.Pharmacol.Exp.Ther.287. 1063-1067 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakaguchi H., Yokokura H., Terada O., Naito Y., Nimura Y., and Hidaka H.: "Unique inhibitory action of the synthetic compound 2- [N-(2-aminoethyl)-N-(5-isoquinolinesulfonyl)] amino-N- (4-chlorocinnayl) -N-methylbenzylamine (CKA-1306) against calcium/calmodulin-dependent protein kinase I" Biochem.Pharmacol.56. 329-334 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gray NS., Wodicka L., Thunnissen AM., Norman TC., Kwon S., Espinoza FH., Morgan DO., Barnes G., LeClerc S., Meijer L., Kim SH., Lockhart DJ., and Schultz PG.: "Exploiting chemical libraries, structure and genomics in the search for kinase inhibitors." Science. 281 (5376). 533-538 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Niki I.: "Ca^<2+> signalling and the insulin secretory cascade in pancreatic beta-cell." Jap.J.Pharmacol.(in print). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ono-Saito N., Niki I., and Hidaka H.: "H-series protein kinase inhibitors, and a case of a H-series inhibitor for clinical application." Pharmacol.Therap.(in print). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iida Y.: "Myosin light chain phosphorylation controls insulin secretion at a proximal step in the secretory cascade." Am.J.Physiol.(Endocrinol.Metab.36). 273. E789-E782 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kabir N.: "Immunocytochemical detection and spatial distribution of myosin light-chain kinase in preimplantation mouse embryos." J.Exp.Zool.278. 147-155 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe Y.: "Identification of a specific amino acid cluster in the calmodulin-binding domain of the neuronal nitric oxide synthase" FEBS Lett.403. 75-78 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naito Y.: "Isoform-specific activation and structural diversity of CaM kinase I" J.Biol.Chem.272. 32704-32708 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yokokura H.: "Isolation and comparison of rat cDNAs encoding Ca^<2+>/calmodulin-dependent protein kinase I isoforms." Biochim.Biophys.Acta(Short sequence paper). 1338. 8-12 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hidaka H.: "Cascade activation of the calmodulin kinase family." in Signal Transduction in Health and Disease,edited by J.Corbin Raven Press.New York,U.S.A.(Proceedings of the 9th International Conference on Second Messengers and Phosphoproteins.Oct.27-Nov.1.1995.Nashville.U.S.A.). 151-157 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 渡邊泰男: "NOSの分子生物学的解析による構造機能活性相関ならびに活性化メカニズムの検討" 日本薬理学雑誌(Folia Pharmacol.Jpn.). 110. 69-75 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Schutte B.: "The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics." Exp Cell Res.236・1. 4-15 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] De Azevedo WF.: "Inhibition of cyclin-dependent kinases by purine analogues: crystal structure of human cdk2 complexed with roscovitine." Eur.J.Biochem.243・1-2. 518-526 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Meijer L.: "Biochemical and cellular effects of roscovitine,a potent and selective inhibitor of the cyclin-dependent kinases cdc2,cdk2 and cdk5." Eur.J.Biochem.243・1-2. 527-536 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Havlicek L.: "Cytokinin-derived cyclin-dependent kinase inhibitors: synthesis and cdc2 inhibitory activity of olomoucine and related compounds." J.Med.Chem.40・4. 408-412 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Pippin JW.: "Direct in vivo inhibition of the nuclear cell cycle cascade in experimental mesangial proliferative glomerulonephritis with Roscovitine,a novel cyclin-dependent kinase antagonist." J.Clin.Invest.100・10. 2512-2520 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Krucher NA.: "The cyclin-dependent kinase (cdk) inhibitors,olomoucine and roscovitine,alter the expression of a molluscan circadian pacemaker." Cell Mol.Neurobiol.17・5. 495-507 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Meijer L.: "Chemical inhibitors of cyclin-dependent kinases." Methods Enzymol.283. 113-128 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Planchais S.: "Roscovitine,a novel cyclin-dependent kinase inhibitor,characterizes restriction point and G2/M transition in tobacco BY-2 cell suspension." Plant J.12・1. 191-202 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe H.: "An essential role of myosin light-chain kinase in the regulation of agonist-and fluid-flow-stimulated Ca^<2+> influx in endothelial cells." FASEBJ.12. 341-348 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Niwa T.^-: "Acetylcholine activates intracellular movement of insulin granules in the pancreatic β-cells via IP3-dependent mobilization of intracellular Ca^<2+>" Diabetes. 47. 1699-1706 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 仁木一郎: "インスリン顆粒運動の制御と細胞内Ca^<2+>の分業仮説." Peptide Hormones in Pancreas. 18. 112-116 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe Y.: "Neuronal nitric oxide synthase-membrane phospholipid interaction" Arch.Biochem.Biophys.358. 68-73 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe Y.: "Inter-isoformal regulation of nitric oxide synthase through heteromeric dimerization" Biochim.Biophys.Acta. 1388. 199-208 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishio M.: "HMN-1180,a small molecule inhibitor of neuronal nitric oxide synthase" J.Pharmacol.Exp.Ther.287. 1063-1067 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakaguchi H.: "Unique inhibitory action of the synthetic compound 2-[N-(2-aminoethyl)-N-(5-isoquinolinesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine(CKA-1306)against calcium/calmodulin-dependent protein kinase I" Biochem.Pharmacol.56. 329-334 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Gray NS.: "Exploiting chemical libraries,structure and genomics in the search for kinase inhibitors." Science. 281・5376. 533-538 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Niki I.: "Intracellular Ca^<2+> receptors in the pancreatic β-cell." Molec.Cell Biochem.(in print). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Niki I.: "Ca^<2+> signalling and the insulin secretory cascade in pancreaticbeta-cell." Jap.J.Pharmacol.(in print). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ono-Saito N.: "H-series protein kinase inhibitors and a case of a H-series inhibitor for clinical application." Pharmacol.Therap.(in print). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 丹羽多恵: "グルコースによるインスリン分泌顆粒運動の活性化機構." Peptide Hormones in Pancreas. (in print). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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