| Project/Area Number |
09044284
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
KITAJIMA Ken Nagoya Univ., School of Agri.Sci., Associate Professor, 農学部, 助教授 (80192558)
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| Co-Investigator(Kenkyū-buntansha) |
COLLEY Karen J. Univ.of Illinois, School of Medicine, Associate Professor, 医学部, 助教授
ROTH Jurgen Univ.of Zurich, Division of Pathology, Professor, 病理部門, 教授
LENNARZ William J. State Univ.of New York, Dept.Biochem.Cell Biol., professor, 生化学細胞生物学部門, 教授
INOUE Yasuo Academia Sinica (Taiwan), Inst.Biol.Chem., Professor, 生化学研究所, 教授
MATSUDA Tsukasa Nagoya Univ., School of Agri.Sci., Professor, 農学部, 教授 (20144131)
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| Project Period (FY) |
1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1997: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | POLYSIALIC ACID / KDN / SULFATED CLYACAN / CELL ADHESION / FERTILIZATION / KDN-CLEAVING ENZYME / BIOSYNTHESIS OF SIALIC ACID / FUNCTION OF SIALIC ACID |
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| Research Abstract |
The objective of this research project was to elucidation of functions of acidic glycan chains of cell surface glycoconjugates in cell recognition and adhesion of animal cells. The following results were obtained :
1.Clarification of biosynthetic mechanism of oligo/polysialic acid units in glycoproteins on the cell surface : (1) Based on transfection experiments of cDNA for trout polysialoglycoprotein (PSGP) that contains polysialyl residues on the O-linked glycan chains (O-linked polySia) into various mammalian cells, biosynthetic mechanism of O-linked polySia was shown to be different from that of N-linked glycan chains that are present on glycoproteins such as neural cell adhession molecule ; (2) Several cDNA clones for polysialyltransferases that are involved in formation of O-linked polySia on trout PSGP were obtained, and their structural analysis are now underway ; (3) Use of various monoclonal antibodies specific to oligomeric structures of N-acetylneuraminic acid (Neu5Ac) enabl
… More
ed to reveal that oligoNeu5Ac-containing glycoproteins were found in mammalian sera and brains. Some of these glycoproteins were identified as known glycoproteins ; (4) Novel oligomeric structure of N-glylcolyneuramic acid (Neu5Gc) was first found in mammalian glycoproteins using newly developed chemical and immunochemical methods.
2.Role of acidic glycan chains in gamete interactions at fertilization : (1) An assay method of assessing the deaminoneuraminic acid (KDN) residue-dependent sperm-egg binding was developed for detecting the egg receptor glycoproteins for sperm in trout, and direct involvement of KDN residues in sperm binding to eggs are now being examined ; (2) An avian homologue of mouse zona pellucida glycoprotein C (ZPC) was found and its cDNA cloning was done.
3.Elucidation of regulatory roles of polySia- or KDN-containing glycan chains in growth activity of tum or cells : KDN-glycan chains were shown to be a developmental stage specific actigen as well as a tumor antigen in human lung cells. Development of monoclonal antibodies to detect the KDN-glycosides is now underway. Less
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