| Project/Area Number |
09044310
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Biological pharmacy
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| Research Institution | Okayama University |
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Principal Investigator |
TSUCHIYA Tomofusa Okayama University, Fac. Pharm. Sci. Professor, 薬学部, 教授 (80012673)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Teruo Okayama University, Gene. Res. Center, Res. Associate, 遺伝子実験施設, 助手 (80304327)
NEGISHI Kazuo Okayama University, Gene. Res. Center, Asso. Professor, 遺伝子実験施設, 助教授 (70116490)
MIZUSHIMA Tohru Okayama University, Fac. Pharm. Sci. Asso. Professor, 薬学部, 助教授 (00264060)
KONINGS Wil, N University of Groningen, Dept. Biology, Professor, 生物学部, 教授
WISLON Thomas, H Harvard University, Medical School, Professor, 医学部, 教授
KONINGS Wil フローニンヘン大学, 生物学部, 教授
WILSON Thoma ハーバード大学, 医学部, 教授
島本 整 広島大学, 生物生産学部, 助教授 (90187443)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | Active transport / Structure and function / Unity and diversity / Symporter / Antiporter |
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| Research Abstract |
It is important to analyze many transport systems for the investigation of unity and diversity in structure, function and mechanism in active transport proteins. Fortunately, we have found and investigated many active transport systems in bacterial cells so far. In the past two years, we have obtained the following results.
1) We analyzed many NaィイD1+ィエD1-coupled transport systems, mainly NaィイD1+ィエD1/melibiose symporter and NaィイD1+ィエD1/serine symporter of Escherichia coli and some other bacteria. We characterized the symporters from biochemical view point and genetic view point. We established methods for over production, large-scale purification and reconstitution of the NaィイD1+ィエD1/melibiose symporter protein and NaィイD1+ィエD1/serine symporter protein. This opened up a way for further analysis in structure, function and mechanism an active transport proteins.
2) We identified regions and amino acid residues important or necessary for ion-coupling or substrate recognition in the NaィイD1+ィエD1/melibiose symporter protein through mutant analysis and site-directed mutagenesis. Homology search and motif analysis were also useful for such analysis.
3) We found new transport systems and cloned their genes in some bacteria. Biochemical and genetic analyses further revealed unity and diversity in transport proteins.
Collaborations with Prof. Wilson and Prof. Konings were very valuable foe this project.
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