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Molecular cytobiological study on hippocampal LTP and LTD

Research Project

Project/Area Number 09044324
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKumamoto University

Principal Investigator

MIYAMOTO Eishichi  Kumamoto University, School of Medicine, Pharmacology, Professor, 医学部, 教授 (50109659)

Co-Investigator(Kenkyū-buntansha) MULLER Dominique  Universite de Geneve, Faculte de Medecine, Pharmacology, Professor, 医学部, 教授
SODERLING Th  オレゴン健康科学大学, ボラム研究所, 教授
KASAHARA Jiro  Kumamoto University, School of Medicine, Pharmacology, Instructor, 医学部, 助手 (10295131)
YAMAMOTO Hideyuki  Kumamoto University, School of Medicine, Pharmacology, Assistant Professor, 医学部, 講師 (60191433)
FUKUNAGA Kohji  Kumamoto University, School of Medicine, Pharmacology, Associate Professor, 医学部, 助教授 (90136721)
SODERLING Thomas R  Oregon Health Sciences University, Vollum Institute, Professor
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordslong-term potentiation / hippocampus / learning / memory / CaM kinase II / MAP kinase / protein phosphatase / protein dephosphorylation / シナプス伝達長期増強 / 後シナプス細胞 / 蛋白質燐酸化反応 / 遺伝子発現
Research Abstract

The purposes of the study is to elucidate the mechanisms of learning and memory of animals on the basis of molecular cytobiological methodology. When electrical high frequency stimulation (HFS) is given to synapses, potentiation of synaptic activities lasts for several hours or longer. This phenomenon is called long-term potentiation(LTP) and considered to be a model for learning and memory of animals. The elucidation of the mechanisms of LTP will give a clue to understand the higher activity of the brain. It has been recently reported that protein phosphorylatiorr especially Ca^2/calmodulin-dependent protein kinaseII (CaM kinase II) is involved in induction and maintenance of LTP in the hippocampus. In an attempt to examine the molecular mechanisms of LTP in the hippocampus, we analyzed LTP.
1)Activation of CaM kinase II and mitogen-activated protein kinase (MAP kinase) was analyzed during LTP induction by the in-gel assay with myelin basic protein as a substrate. MAP kinase was activated during LTP induction. The peak of the activation was observed 3 min after HFS and rapidly decreased to the original level within 10 mm. When 50 muM PD098059, a MAP kinase kinase inhibitor, was previously given to hippocampal slices, LTP induction and activation of both CaM kinase II and MAP kinase were inhibited. However, addition of 30 muM PD098059 to slices did not affect both LTP and activation of CaM kinase II but did inhibit activation of MAP kinase. These results indication that LTP induction is correlated with activation of CaM kinase II, but not with activation of MAP kinase.
2)Calyculin A-sensitive protein phosphatase (PP) activity, which includes PP1 and PP2 was inhibited during LTP induction. The autoradiography and immunoblot analyses showed that phosphorylation of the regulatory subunit of PP2A is phosphorylated with a concomitant decrease in PP activity.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (98 results)

All Other

All Publications (98 results)

  • [Publications] Motohiro Morioka: "Calcineurin in the adult rat hippocampus : Different distribution in CA1 and CA3 subfields." Neuroscience. 78. 673-684 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masunobu Sugimura: "DY-9760e, a novel calmodulin antagonist with cytoprotective action." Eur. J.Pharmacol.336. 99-106 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 御子紫克彦: "細胞内カルシウムホメオスタシスと細胞機能" 細胞工学. 16. 10-15 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 福永浩司: "CaMキナーゼIIと神経機能" 細胞工学. 16. 55-63 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本秀幸: "脳のSer/Thrホスファターゼの役割" 細胞工学. 16. 210-216 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 芳賀達也: "序" 蛋白質核酸酵素. 42. 199 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "細胞内シグナル伝達「概説」" 蛋白質核酸酵素. 42. 349-352 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 堀田凱樹: "座談会「脳における遺伝子発現と調節-現状と展望」" 蛋白質核酸酵素. 42. 589-607 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "プロテインキナーゼ活性化薬,阻害薬…(1)" Clinical Neuroscience. 15. 346-347 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "プロテインキナーゼ活性化薬,阻害薬…(2)セリン/スレオニンキナーゼ" Clinical Neuroscience. 15. 470-471 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "プロテインキナーゼ活性化薬,阻害薬…(3)チロシンキナーゼ" Clinical Neuroscience. 15. 582-583 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "細胞内情報伝達系と可塑性" Clinical Neuroscience. 15. 1119-1121 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本秀幸: "カルシニューリンの構造と機能" 実験医学. 15. 592-597 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "日本薬理学会創立70周年記念座談会「21世紀への薬理学と薬理学会」" 日薬理誌. 110. 225-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katsuro Iwase: "Precise distribution of neuronal nitric oxide synthase mRNA in the rat brain revealed by non-radioisotopic in situ hybridization." Mol. Brain Res.53. 1-12 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Kimura: "Basic fibroblast growth factor stimulates phosphorylation of CCAAT/enhancer-binding protein δ by activation of mitogen-activated protein kinase." Biomedical Res.19. 65-75 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuharu Oishi: "Hindlimb suspension induces the expression of multiple myosin heavy chain isoforms in single fibres of the rat soleus muscle." Acta Physiol. Scand.162. 127-134 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seiji Inui: "Ig receptor binding protein 1(α4) is associated with a rapamycin-sensitive signal transduction in lymphocytes through direct binding to the catalytic subunit of protein phosphatase 2A." Blood. 92. 539-546 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hideyuki Yamamoto: "The antibody specific for myristoylated alanine-rich C kinase substrate phosphorylated by protein kinase C Activation of protein kinase C in smooth muscle cells in human coronary arteries." Arch. Biochem. Biophys.359. 151-159 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Motohiro Morioka: "Serine/threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect." Biochem. Biophys. Res. Commun.253. 342-345 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kohji Fukunaga: "Role of MAP kinase in neurons." Mol. Neurobiol.16. 79-95 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 田村眞理: "序" 蛋白質核酸酵素. 43. 923 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本秀幸: "細胞骨格蛋白質の脱燐酸化反応-微小管蛋白質の燐酸化と脱燐酸化反応を中心にして-" 蛋白質核酸酵素. 43. 1004-1012 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 福永浩司: "神経可塑性とプロテインホスファターゼ" 蛋白質核酸酵素. 43. 1062-1071 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "概説" 蛋白質核酸酵素. 43. 1636-1639 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 後藤恵: "カルシニューリンの構造と細胞機能調節" 蛋白質核酸酵素. 43. 1706-1714 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本秀幸: "Ca^<2+>、カルモデュリン依存性蛋白リン酸化反応による脳由来神経栄養因子産生の調節機構" 医学のあゆみ. 別冊. 238-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本秀幸: "選択的mRNAスプライシングによって生じるCa^<2+>/カルモジュリン依存性プロテインキナーゼII可変領域の多様性とその生理的意義:アイソフォームの細胞内局在決定機構との関連について" 生化学. 70. 1349-1354 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yusuke Takeuchi: "Nuclear localization of the δ subunit of Ca^<2+>/calmodulin-dependent protein kinase II in rat cerebellar granule cells." J.Neurochem.72. 815-825 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masao Ohmitsu: "Phosphorylation of myristoylated alanine-rich protein kinase C substrate by mitogen-activated protein kinase in cultured rat hippocampal neurons following stimulation of glutamate receptors." J.Biol. Chem.274. 408-417 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuya Matsumoto: "Cloning from insulinoma cells of synapsin I associated with insulin secretory granules." J.Biol. Chem.274. 2053-2059 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kohji Fukunaga: "Current studies on a working model of CaM kinase II in hippocampal long-term potentiation and memory." Jpn. J.Pharmacol.79. 7-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M Nakai: "Amyloid beta protein (25-35) phosphorylates MARCKS through tyrosine kinase-activated protein kinase C signaling pathway in microglia." J.Neurochem.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Jiro Kasahara: "Differential effects of a calcineurin inhibitor on glutamate-induced phosphorylation of Ca^<2+>/calmodulin-dependent protein kinases in cultured rat hippocampal neurons." J.Biol. Chem.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Eishichi Miyamoto: "Role of Ca^<2+>/calmodulin-dependent protein kinase II in neuronal regulation (In : Kinases and Phosphatases in Lymphocyte and Neuronal Signaling)" Springer-Verlag, Tokyo, 10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "第2編「薬物と生体機能制御系」第5章「受容体と細胞内情報伝達系」(標準薬理学)" 医学書院,東京, 11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 蛯原賢司: "インスリン分泌調節機構におけるプロテインホスファターゼ2Bの関与(Peptide Hormones in Pancreas, 17)" BIOMEDICAL RESEARCHFOUNDATION,東京, 5 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuyuki Yanagihara: "Fact or affecting a denal medillary catec holamine biosynthes is andrele as e (In : Catecholamines : Bridging Basic Science with Clinical Medicine, Advances in Pharmacology, Vol.42)" Academic Press, San Diego, 4 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuyuki Yanagihara: "Short-term regulation of catecholamine synthesis by Ca^<2+>/calmodulin-dependent protein kinase II (In : The Adrenal Chromaffin Cell, Archetype and Exemplar of Cellular Signaling in Secretory Control)" Hokkaido University Press, Sapporo, 5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "神経の可塑性と神経伝達物質(神経伝達物質update-基礎から臨床まで)" 中外医学社,東京, 5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 大野茂男: "17.シグナル伝達のカスケードとネットワーク(細胞機能と代謝マップ:II.細胞の動的機能(日本生化学会・編)" 東京化学同仁,東京, 18 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮本英七: "脳可塑性(脳と神経:分子神経生物科学入門)" 共立出版,東京, 7 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Morioka, S.Nagahiro, K.Fukunaga, E.Miyamoto and Y.Ushio: "Calcineurin in the adult rat hippocampus : Different distribution in CA1 and CA3 subfields." Neuroscience. 78. 673-684 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Sugiyama, T.Sato, W.Nakayama, Y.Morishima, K.Fukunaga, M.Ohmitsu, E.Miyamoto and Y.Shirasaki: "DY-9760e, a novel calmodulin antagonist with cytoprotective action." Eur.J.Pharmacol.336. 99-106 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Mikoshiba and E.Miyamoto: "Homeostasis of intracellular calcium and its cellular functions.(in Japanese)(Special Issure"Calsium Signaling and Cellular Functions"[eds. : K.Mikoshiba and E.Miyamoto])" Saibo Kogaku. 16. 10-15 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Fukunaga and E.Miyamoto: "CaM kinase II and functions of nervous system.(in Japanese)(Special Issue"Calcium Signaling and Cellular Functions"[eds. : K.Mikoshiba and E.Miyamoto])" Saibo Kogaku. 16. 55-63 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Yamamoto and E.Miyamoto: "Role of Ser/Thr phosphatase in the brain.(in Japanese)(Special Issue"Cellular Biology of Protein Phosphatase, Advanced Elucidation of Role of Protein Dephosphorylation"[Supervisor : H.Yakura])" Saibo Kogaku. 16. 210-216 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Haga, M.Mishina, K.Uyemura and E.Miyamoto: "Introduction(in Japanese)(Special Issue"Signal Transduction in the Brain : Functional Components and Reactions in Neurons"[eds. : T.Haga, M.Mishina, K.Uyemura and E.Miyamoto])" Tanpakushitsu Kakusan Kohso. 42. 199 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto: "General view on signal transduction in the cells.(in Japanese)(Special Issue"Signal Transduction in the Brain : Functional Components and Reactions in Neurons"[eds. : T.Haga, M.Mishina, K.Uyemura and E.Miyamoto])" Tanpakushitsu Kakusan Kohso. 42. 349-352 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Hotta, M.Mishima, T.Yamamori, D.Saffen and E.Miyamoto: "Round talk discussion : Gene expression and regulation in brain-Present state and prospects.(in Japanese)(Spcial Issue"Signal Transduction in the Brain : Functional Components and Reactions in Neurons"[eds. : T.Haga, M.Mishina, K.Uyemura and E.Miyamoto])" Tanpakushitsu Kakusan Kohso. 42. 589-607 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto: "Activators and inhibitors of protein kinases(1).(in Japanese)" Clinical Neuroscience. 15. 346-347 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto: "Activators and inhibitors of protein kinases(2)Ser/Thr kinases.(in Japanese)" Clinical Neuroscience. 15. 470-471 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto: "Activators and inhibitors of protein kinases(3)Tyrosine kinases.(in Japanese)" Clinical Neuroscience. 15. 582-583 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto and K.Fukunaga: "Signal transduction and brain plasticity.(in Japanese)" Clinical Neuroscience. 15. 1119-1121 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Yamamoto and E.Miyamoto: "Structure and function of calcineurin.(in Japanese)(Special Issue"New Functions of Calcineurin-Involvement in Nerve Plasticity and Apoptosis")" Jikken Igaku. 15. 592-597 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto: "Momentous round table discussion of the 70th anniversary of the Japanes Pharmacological Society"Pharmacology and The Japanese Pharmacological Society in the 21st Century".(in Japanese)" Folia Pharmacol.Jpn.110. 225-242 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Iwase, K.Iyama, K.Akagi, S.Yano, K.Fukunaga, E.Miyamoto, M.Mori and M.Takiguchi: "Precise distribution of neuronal nitric oxide synthase mRNA in the rat brain revealed by non-radioisotopic in situ hybridization." Mol.Brain Res.53. 1-12 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Kimura, K.Fukunaga, Y.Ushio and E.Miyamoto: "Basic fibroblast growth factor stimulates phosphorylation of CCAAT/enhancer-binding protein delta by activation of mitogen-activated protein kinase." Biomedical Res.19. 65-75 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Oishi, A.Ishihara, H.Yamamoto and E.Miyamoto: "Hindlimb suspension induces the expression of multiple myosin heavy cahin isoforms in single fibres of the rat soleus muscle." Acta Phsiol.Scand.162. 127-134 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Inui, H.Sanjo, K.Maeda, H.Yamamoto, E.Miyamoto and N.Sakaguchi: "Ig receptor binding protein 1 (alpha4) is associated with a rapamycin-sensitive signal transduction in lymphocytes through direct binding to the catalytic subunit of protein phosphatase 2A." Blood. 92. 539-546 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Yamamoto, T.Matsumura, K.Kugiyama, Y,Oishi, N.Ogata, H.Yasue and E.Miyamoto: "The antibody specific for myristoylated alanine-rich C kinase substrate phosphorylated by protein kinase C : Activation of protein kinase C in smooth muscle cells in human coronary arteries." Arch.Biochem.Biophys.359. 151-159 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Morioka, K.Fukunaga, T.Kawano, S.Hasegawa, K.Korematsu, Y.Kai, J.Hamada, E.Miyamoto and Y.Ushio: "Serine/threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect." Biochem.Biophys.Res.Commun.253. 342-345 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Fukunaga and E.Miyamoto: "Role of MAP kinase in neurons." Mol.Neurobiol. 16. 79-95 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Tamura, H.Yakura, M.Takeda and E.Miyamoto: "Introduction(in Japanese)(Special Issue"Structure and Function of Protein Phosphatases")." Tanpakushitsu Kakusan Kohso. 43. 923 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Yamamoto and E.Miyamoto: "Dephosphorylation of cytoskeletal proteins-Phosphorylation and dephosphorylation of microtubule proteins-(in Japanese)(Special Issue"Structure and Function of Protein Phosphatases")." Tanpakushitsu Kakusan Kohso. 43. 1004-1012 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Fukunaga and E.Miyamoto: "Neuronal plasticity and protein phosphatases(in Japanese)(Special Issue "Structure and Function of Protein Phosphatases")." Tanpakushitsu Kakusan Kohso. 43. 1062-1071 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E Miyamoto: "Overview(in Japanese)(Special Issu"Calcium Ion and Signal Transduction")." Tanpakushitsu Kakusan Kohso. 43. 1636-1639 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Goto, H.Yamamoto, T.Hamasaki and E.Miyamoto: "Calcineurin-Structure and regulation of the cellular functions.(in Japanese)(Special Issue"Calcium Ion and Signal Transduction")." Tanpakushitsu Kakusan Kohso. 43. 1706-1714 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Yamamoto, Y.Takeuchi and E.Miyamoto: "Regulation of expression of brain-derived neurotrophic factor by Ca^<2+> calmodulin-dependent protein phosphorylation.(in Japanese)(Special Issue"The Regulation of Neural Cell Death")." Igaku-no-Ayumi. 238-242. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Yamamoto, Y.Takeuchi and E.Miyamoto: "Variable region of the Ca^<2+>/calmodulin-dependent protein kinase II generated by alternative mRNA splicing : its role in the intracellular localization of different isoforms." Seikagaku. 70. 1349-1354 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Takeuchi, H.Yamamoto, K.Matsumoto, T.Kimura, S.Katsuragi, T.Miyakawa and E.Miyamoto: "Nuclear・localization of the delta subunit of Ca^<2+>/calmodulin-dependent protein kinase II in rat cerebellar granule cells." J.Neurochem.72. 815-825 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Ohmitsu, K.Fukunaga, H.Yamamoto and E.Miyamoto: "Phosphorylation of myristoylated alanine-rich protein kinase C substrate by mitogen-activated protein kinase in cultured rat hippocampal neurons following stimulation of glutamate receptors." J.Biol.Chem.274. 408-417 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Matsumoto, K.Ebihara, H.Yamamoto, H.Tabuchi, K.Fukunaga, M.Yasunami, H.Ohkubo, M.Shichiri and E.Miyamoto: "Cloning from insulinoma cells of synapsin I associated with insulin secretory granules." J.Biol.Chem.274. 2053-2059 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Fukunaga and E.Miyamoto: "Current studies on a working model of CaM kinase II in hippocampal long-term potentiation and memoery." Jpn.J.Pharmacol. 79. 7-15 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Nakai, K.Hojo, K.Yagi, N.Saito, T.Taniguchi, A.Terashima, T.Kawamata, T.Hashimoto, K.Maeda, M.Gschwendt, H.Yamamoto, E.Miyamoto and C.Tanaka: "Amyloid beta protein(25-35)posphorylates MARCKS through tyrosine kinase-activated protein kinase C signaling pathway in microglia." J.Neurochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] J.Kasahara, K.Fukunaga and E.Miyamoto: "Differential effects of a calcineurin inhibitor on glutamate-inducced phosphorylation of Ca^<2+>/calmodulin-dependent protein kinases in cultured rat hippocampal neurons." J.Biol.Chem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto, K.Fukunaga, H.Yamamoto, S.Yano and D.Muller: "Role of Ca^<2+>/calmodulin-dependent protein kinase II in neuronal regulation" Kinases and Phosphatases in Lymphocyte and Neuronal Signaling(ed. : H.Yakura). Springer-Verlag, Tokyo. 252-261 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto: "Receptors and intracellular signal transduction." Standard Pharmacology(5th Ed.)(in Japanese), (compi. : A.Ebihara ; eds. : M.Katori, T.Imai)). Igaku-Shoin, Tokyo. 48-58 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ebihara, K.Fukunaga, K.Matsumoto, T.Shirotani, H.Kishikawa, M.Shichiri and E.Miyamoto: "Involvement of protein phosphatase 2B in regulation of insulin secretion." Peptide Hormones in Pancreas, 17(in Japanese). BIOMEDICAL RESEARCH FOUNDATION,Tokyo. 50-54 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Yanagihara, F.Izumi, E.Miyamoto and M.Oka: "Factors affecting adrenal medullary catecholamine biosynthesis and release." Catecholamines : Bridging Basic Science with Clinical Medicine, Advances in Pharmacology, Vol.42(eds. : D.S.Goldstein, G.Eisenhofer and R.McCarty). Academic Press, San Diego. 18-21 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Yanagihara, M.Tsutsui, E.Miyamoto and F.Izumi: "Short-term regulation of catecholamine synthesis by Ca^<2+>/calmodulin-dependent protein kinase II." The Adrenal Chromaffin Cell, Archetype and Exemplar of Cellular Signaling in Secretory Control(eds. : T.Kanno, Y.Nakazato and K.Kumakura). Hokkaido University Press, Supporo. 243-247 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamto: "Brain plasticity and neurotransmitters." Neurotransmitters Update-From Basic Research to Clinical Aspect-(in Japanese).(Ed. : S.Nakamura). Chugai-Igaku-sha, Tokyo. 58-62 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Ohno and E.Miyamoto: "Cascade of signal transduction and network." Cellular Functions and Metabolic Map.II.Dynamic functions of cells(in Japanese), (Eds. : Y.Nishizuka, Y.Sekiyama, A.Ikai, T.Ishimura and A.Kawaguchi). 119-136 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] E.Miyamoto and K.Fukunaga: "Brain plasticity." Brain and Nerve : A Manual of Molecular Neurobiology(in Japanese), (Eds. : A.Kaneko, K.Kawamura and K.Uyemura). Kyoritsu-Shuppan, Tokyo. 277-283 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katsuro Iwase: "Precise distribution of neuronal nitric oxide synthase mRNA in the rat brain revealed by non-radioisotopic in situ hybridization." Mol.Brain Res.53. 1-12 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Seiji Inui: "Ig receptor binding protein I(α4)is associated with a rapamycin-sensitive signal transduction in Iymphocytes through direct binding to the catalytic subunit of protein phosphatase 2A." Blood. 92. 539-546 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hideyuki Yamamoto: "The antibody specific for myristoylated alanine-rich C Kinase substrate phosphorylated by protein kinase C:Activation of protein kinase C in smooth muscle cells in human coronary arteries." Arch.Biochem.Biophys.359. 151-159 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yusuke Takeuchi: "Nuclear localization of the δ subunit of Ca^<2+>/calmodulin-dependent protein kinase II in rat cerebellar granule cells." J.Neurochem.72. 815-825 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masao Ohmitsu: "Phosphorylation of myristoylated alanine-rich protein kinase C substrate by mitogen-activated protein kinase in cultured rat hippocampal neurons following stimulation of glutamate receptors." J.Biol.Chem.274. 408-417 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kazuya Matsumoto: "Cloning from insulinoma cells of synapsin I associated with insulin secretory granules." J.Biol.Chem.274. 2053-2059 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuyuki Yanagihara: "Factors affecting adrenal medullary catecholamine biosynthesis and release,In:Catecholamines:Bridging Basic Science with Clinical Medicine,Advances in Pharmacology,Vol.42" Academic Press,San Diego, 1084 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Motohiro Morioka: "Calcineurin in the adult rat hippocampus:Different distribution in CA1 and CA3 subfields" Neuroscience. 78. 673-684 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masanobu Sugimura: "DY-9760e,a novel calmodulin antagonist with cytoprotective action" Eur.J.Pharmacology. 336. 99-106 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 御子柴 克彦: "細胞内カルシウムホメオスタシスと細胞機能" 細胞工学. 16. 10-15 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 福永 浩司: "CaMキナーゼIIと神経機能" 細胞工学. 16. 55-63 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山本 秀幸: "脳のSer/Thrホスファターゼの役割" 細胞工学. 16. 210-215 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 宮本 英七: "細胞内情報伝達系と可塑性" CLINICAL NEUROSCIENCE. 15. 1119-1121 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Eishichi Miyamoto: "Role of Ca^<2+>/calmodulin-dependent protein kinase II in neuronal regulation,In:Kinases and Phosphatases in Lymphocyte and Neuronal Signaling" Springer-Verlag,Tokyo(Ed.H.Yakura), 10 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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