Development of flavivirus-cross-reactive subunit vaccines
| Project/Area Number |
09044344
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research . |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases (1998)
Kinki University (1997) |
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Principal Investigator |
KURANE Ichiro National Institute of Infectious Diseases・Department of Virology I,Director, ウイルス第一部, 部長 (90278656)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASAKI Tomohiko National Institute of Infectious Diseases Department of Virology I,Senior Scient, ウイルス第一部, 主任研究官 (20221351)
ENNIS Franci マサチューセッツ大学, 医学部, 教授
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Flavivirus / Dengue virus / Japanese encephalitis virus / Vaccine / Cytotoxic T lymphocyte / デンブウイルス / デンフウウルス / エピトープ |
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| Research Abstract |
The aim of the present researh is to examine whether it is possible to develop vaccines which induce protective immunity against multiple flaviviruses. We analyzed flavivirus-cross-reactive T lymphocyte responses. We first analyzed CD8+ cytotoxic T lymphocytes (CTL) responses to the epitope on the NS3 protein of dengue viruses. Mice were infected with dengue virus type 2. CTL induced by the infection recognized the epitope located on the amino acids 289 to 306 on the NS3. Most of the CTL recognizing this epitope were cross-reactive for dengue virus type 2 and type 4. When mice were infected with dengue virus type, CTL were cross-reactive for all the 4 serotyoes of dengue viruses, but also recognized the epitope located on the amino acids 289 to 306 on the NS3. CTL clones established from the mice infected with dengue virus type 3 were cross-reactive for other flaviviruses such as Kunjin virus and Murray Valley encephalitis virus. It is generally accepted that virus-specific CTL are important for recovery fromvirus infections. Thus, the results suggest that development of vaccines which induce protective immunity against multiple flaviviruses may be possible. It is of interest that flavivirus-cross-reactivity differs depending of the serotype of the infecting dengue viruses. This result is consistent with the observation that rate of the development of dengue hemorrhagic fever differs depending on the serotypes in the first and second dengue viral infections. Human CD4+ T cell clones established from Japanese encephalitis-immune donors Included those cross-reactive for Japanese encephalitis and West Nile viruses. Thus, flavivirus-cross-reactive T cells are also present. in humans.
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Report
(3 results)
Research Products
(16 results)
