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組み換え近交系マウスを用いた肺腫瘍抵抗遺伝子の研究

Research Project

Project/Area Number 09253227
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionKyoto University

Principal Investigator

日合 弘  京都大学, 医学研究科, 教授 (10073131)

Co-Investigator(Kenkyū-buntansha) 西村 正彦  浜松医科大学, 医学部, 助教授 (20073661)
阿不江 ぱ塔雨  京都大学, 医学研究科, 助手 (60293875)
福本 学  京都大学, 医学研究科, 助教授 (60156809)
Project Period (FY) 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1997: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywords肺癌抑制遺伝子 / 組み換え近交系 / SMXA / Par1 / Par3 / QTL解析 / nPKCη
Research Abstract

肺腫瘍高感受性のA/Jと低感受性のSM/J系の間で26系統からなる組み換え近交系SMXAを樹立し、ウレタン誘発肺腫瘍発生率を調べたところ、その1系SMXA24は既知の4つの感受性遺伝子が感受性アレルをもつにも関わらず、強い抵抗性を示した。A/Jへの戻し交配系にウレタンを投与し、発生した肺腫瘍数を定量的形質バラメターとして遺伝解析を行った結果、第11、第12染色体上にそれぞれ有意のQTLを検出した。これらはいずれもSM/J由来アレルが優性で肺腫瘍敷を抑制した。染色体位置から第11染色体上のQTLはDraganiが報告したPar1と同じとみなされたが、第12染色体上のQTLは未報告の抵抗遺伝子でPar3と命名された。マップ位置からPar3の侯補遺伝子はnPKCηである。nPKCηは肺、皮膚で特異的に発現され、肺腫瘍で抑制されることがわかっているが、これまでの検索ではその発現、PCR産物の構造等についてみるべき異常はみつかっていない。
ヒトの肺腺癌材料薬50例についてPar1,Par3の相同染色体部位のLOHを検索したところ、informative caseのうち、前者で50%、後者で30%の頻度でLOHが検出された。比較遺伝学的はヒト固形癌の遺伝的異常を検索するひとつのアプローチである。

Report

(1 results)
  • 1997 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Hiai H.: "genetic medispositin to limuphanas in unce" Pathol.Tvtiu. 46. 707-718 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suwa,H.: "Oveuylnessin of the rho C gene corrilate with mojwssin of dutal a dimocaiciusra of the hamoses" Bsit J.Canu. 77. 147-152 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanuma J.: "Onautilative ttait loci affectiny 4 Nao-inaucol tingne caaisoguesis in the set" Cancer Res. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiai H.: "mapping of nakasso cataiact give nct on nsvuse chaomtz zuxne16." genovics. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Lu-LM.: "Finte-chanaitin 3 atsuin of LEXF R 1 sltauis of sats" Rat Geuone. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Lu,L-M: "Lays gauilan lepuplwatin diveliping in Hupue ctsmijea (SLAch×AKR)F_1 nice" Anei J Hathol. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shisa H, et al.: "A new set secomluinant inbud stsains fetveen LE/Stm and F344" Mammal gerwne. 8. 324-327 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toyokuni,S, et al.: "Quantitative isumuse histohemical atirnusatuin of 8-hydisy 2^1-deoxyguanogsu by a wonoclonal autibody N45,l." Lab.twest.76. 365-374 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kamoto,T.et al.: "Ouantitative tsait loci associatid with mouolinp eppects of sodism L-ascorbate on two stage bladder cauiuogeues in nats" Jpn.J.Cancer Res.88. 633-638 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Abujiang,P, et al.: "Genetic sesis beince to usethane in duced pulmonany adinomas in SMXA R1 stiains." Cancer Res,. 57. 2904-2908 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wang,by, et al.: "Host modifus geus affecting mouse autoisumeing indues by Lpr gene." Amen.J.Pathol.151. 1791-1798 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka,T.: "Inauction afid muchean trauslocation of thiosedoxin of tubalarnecausis and sulfhipasyl dispelities" Lab,Tnvest.77. 145-155 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iida F, et al.: "Ruptise of lens cateract=A movel hereditary secessive cataract moael in the mouse." Exp.Eye Res.64. 107-113 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hattori,Y, et al.: "8-hydroxy-2^1-deoxyguanosine is increased in eyniaermal cello of haerless mice after chinue ultea vislit B exposure." Mvest.Dermatol.107. 733-737 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Higo,K, et al.: "Susceplibility of nude mio-carrying the Fv-4 gene to fniencl muesine loufemio vives induction" T.Visol.71. 750-754 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Lu,L-M.et al.: "Exmession of LECAM-1 and LFA-1 on jve-B lymphoma cells luct mot on meneaplastic pre B cells in SL1Kh nice" Leck,Res.21. 337-342 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toyokuni,S, et al.: "Inductin of a wideravge of C2-12 alduhydi and C17-12 ayloius in the kisny of wister sets after liealmeut with seual" Frce Rad,Biol,Med.22. 1019-1027 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nishiyama,Y, et al.: "Ovesexpression of inteysin-assoeiatid protein (CD4,7)in sat kidney treatin with carcinoge,ferric mitibo a cetate" Jpn,J.cancer Res.88. 120-128 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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