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Intracellular Communication Mechanism for Organization of Photosynthetic Functions through Plant Body

Research Project

Project/Area Number 09274103
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SATO Fumihiko  Kyoto University, Graduate School of Biostudies, Professor, 大学院・生命科学研究科, 教授 (10127087)

Co-Investigator(Kenkyū-buntansha) HASE Toshiharu  Osaka University, Institute for Protein Research, Professor, 蛋白質研究所, 教授 (00127276)
OMATA Tatsuo  Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (50175270)
SUGITA Mamoru  Nagoya University, Center for Gene Research, Professor, 遺伝子実験施設, 教授 (70154474)
NISHIMURA Mikio  Okazaki National Research Institutes, National Institute for Basic Biology, Professor, 基礎生物学研究所, 教授 (80093061)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥186,500,000 (Direct Cost: ¥186,500,000)
Fiscal Year 2000: ¥47,500,000 (Direct Cost: ¥47,500,000)
Fiscal Year 1999: ¥46,000,000 (Direct Cost: ¥46,000,000)
Fiscal Year 1998: ¥47,000,000 (Direct Cost: ¥47,000,000)
Fiscal Year 1997: ¥46,000,000 (Direct Cost: ¥46,000,000)
KeywordsChloroplastic DNA-binding protease / Chloroplastic RNA-binding protein / Chloroplast development and plant growth / PII and NtcA in C / N metabolism / Redox regulation in plastid / Fd / FNR protein interaction / Light regulation of alternative splicing / Trans-acting factors in alternative splicing / 葉緑体形質転換 / 窒素同化と炭素同化のクロストーク / 電子分配 / マイクロボディの機能転換 / 形質転換植物 / 遺伝子破壊
Research Abstract

1. A novel chloroplast nucleoid DNA-binding protein with protease activity, CND41, has been identified and its multiple functions in Rubisco degradation during senescence, chloroplast development in meristem, growth regulation via gibberellin biosynthesis and so on were characterized.
2. The RNA-stablizing activity of chloroplastic RNA-binding proteins (cpRNP) on stroma-accumulating transcripts was found and the target RNA species were identified using chloroplast DNA-microarray.
3. The mechanism of electron transfer around Photosystem I through specific Fd molecules for mesophyll and bundle sheath cells in C4 maize plants was clarified to control the carbon/nitrogen metabolism. Three dimensional structure of Fd/FNR complex was resolved to declare the molecular mechanism of the regulation of electron transfer.
4. Using cyanobacterium as a model, the molecular mechanism of the control of carbon/nitrogen metabolism was clarified ; 2-oxoglutarate was shown to be essential for transcriptional activation by NtcA (the global nitrogen regulator) of the N assimilation genes ; The regulatory domain of the nitrate transporter, involved in downregulation of the transport activity under N-replete conditions, was identified and shown to control the transport activity in concert with PII (the glnB gene product).
5. Regulation of subcellular localization of peroxisomal hydroxypyruvate reductase and chloroplastic ascorbate peroxidase by alternative splicing was established and the trans-acting factor involved in the splicing was isolated. Several mutants with defects in beta-oxidation pathway have been isolated and the mechanism in lipid degradation in glyoxisome has been considerably clarified.

Report

(5 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (208 results)

All Other

All Publications (208 results)

  • [Publications] Nakano, T., Sato, F.et al.: "A novel protein with DNA binding activity from tobacco chloroplast nucleoids"Plant Cell. 9. 1673-1682 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeda, S., Sato, F.et al.: "Cultured green cells of tobacco as a useful material for the study of chloroplast replication"Methods Cell Sci.. 21. 149-154 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murakami, S., Sato, F.et al.: "Protease activity of CND41,a chloroplast nucleoid DNA-binding protein,isolated from cultured tobacco cells"FEBS Lett.. 468. 15-18 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ifuku, K., Sato, F.: "Importance of the N-terminal sequence of the extrinsic 23 kDa polypeptide in photosystem II in ion retention in oxygen evolution"Biochim.Biophys.Acta. 1546. 196-204 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugita, M.et al.: "Targeted deletion of sprA from the tobacco plastid genome indicates that the encoding small RNA is not essential for pre-16S rRNA maturation in plastids"Mol.Gen.Genet.. 257. 23-27 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyagai, T., Sugita, M.et al.: "Transcript analysis of the tobacco plastid operon rps2/atpI/H/F/A reveals the existence of a non-consensus type II(NCII)promoter upstream of the atpI coding sequence"Mol.Gen.Genet.. 257. 299-307 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuda, M., Sugita, M.et al.: "Physiological characterization of RNA-binding protein-deficient cells from Synechococcus sp.strain PCC7942"Plant Cell Physiol.. 40. 1203-1209 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, T., Sugita, M.et al.: "Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs"FEBS Lett.. 460. 437-441 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugita, C., Sugita, M.et al.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp.PCC6301:a soluble 33-kDa polypeptide with high sequence similarity to ribosomal protein S1"Mol.Gen.Genet.. 263. 655-663 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, T., Sugita, M.et al.: "Chloroplast ribonucleoproteins function as a stabilizing factor of ribosome-free mRNAs in the stroma"J.Biol.Chem.. 276. 147-152 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, Y., Sugita, M.et al.: "Dual targeting of phage-type RNA polymerase to both mitochondria and plastids is due to alternative translation initiation in single transcripts"Biochem.Biophys.Res.Commun.. 289. 1106-1113 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, Y., Sugita, M.et al.: "Genomic organization and organ-specific expression of a nuclear gene encoding phage-type RNA polymerase in Nicotiana sylvestris"Gene. 279. 33-40 (2001)

    • Description
      「研究成果報告書概要(和文)」より
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      2001 Final Research Report Summary
  • [Publications] Aichi, M., Omata, T.: "Involvement of NtcB,a LysR family transcription factor,in nitrite activation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp.strain PCC 7942"J.Bacteriol.. 179. 4671-4675 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harano, Y., Omata, T.et al.: "Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp.strain PCC6803 and Synechococcus sp.strain PCC 7942"J.Bacteriol. 179. 5744-5750 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, M., Omata, T.et al.: "Involvement of the C-terminal domain of an ATP-binding subunit in the regulation of the ABC-type nitrate/nitrite transporter of the cyanobacterium Synechococcus sp.strain PCC 7942"J.Biol.Chem.. 272. 27197-27201 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, S., Omata, T.et al.: "cis-Acting sequences required for NtcB-dependent,nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp.strain PCC 7942"J.Bacteriol.. 180. 4080-4088 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, S., Omata, T.et al.: "Nitrite-specific active transport system of the cyanobacterium Synechococcus sp.strain PCC 7942"J.Bacteriol.. 180. 6761-6763 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Rodriguez, R., Omata, T.et al.: "Independence of carbon and nitrogen control in the posttranslational regulation of nitrate transport in the cyanobacterium Synechococcus sp.strain PCC 7942"FEBS Lett.. 432. 207-212 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Omata, T.et al.: "Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp.strain PCC 7942"Proc.Natl.Acad.Sci.USA. 96. 13571-13576 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, S., Omata, T.et al.: "Bicarbonate binding activity of the CmpA protein of the cyanobacterium Synechococcus sp.strain PCC 7942 involved in active transport of bicarbonate"J.Biol.Chem.. 275. 20551-20555 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Omata, T.et al.: "Involvement of a CbbR homolog in low CO_2 induced activation of the bicarbonate transporter operon in cyanobacteria"J.Bacteriol.. 183. 1891-1898 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aichi, M., Omata, T.et al.: "Role of NtcB in activation of the nitrate assimilation genes in the cyanobacterium Synechocystis sp.strain PCC 6803"J.Bacteriol.. 183. 5840-5847 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumura, T., Hase, T.et al.: "A nitrate-inducible ferredoxin in maize roots:genomic organization and differential expression of two nonphotosynthetic ferredoxin isoproteins"Plant Physiol.. 114. 653-660 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, R., Hase, T.et al.: "Cloning of maize ferredoxin III gene:presence of a unique repetitive nucleotide sequence within an intron found in the 5'-untranslated region"Plant Cell Physiol.. 38. 1167-1170 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniguchi, I., Hase, T.et al.: "Electrochemical study of biological functions of particular evolutionary conserved amino acid residues using mutated molecules of maize ferredoxin"Chem.Lett.. 1997. 929-930 (1997)

    • Description
      「研究成果報告書概要(和文)」より
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      2001 Final Research Report Summary
  • [Publications] Yonekura-Sakakibara, K., Hase, T.et al.: "Molecular characterization of tobacco sulfite reductase:enzyme purification,gene cloning and gene expression analysis"J.Biochem.. 124. 615-621 (1998)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akashi, T., Hase, T.et al.: "Comparison of the electrostatic binding sites on the surface of ferredoxin for two ferredoxin-dependent enzymes,ferredoxin-NADP^+ oxidoreductase and sulfite reductase"J.Biol.Chem.. 274. 29399-29405 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumura, T., Hase, T.et al.: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves"Plant Physiol.. 119. 481-488 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okuhara, H., Hase, T.et al.: "Cloning and inactivation of genes encoding ferredoxin- and NADH-dependent glutamate synthase in the cyanobacterium Plectonema boryanum"Plant Physiol.. 120. 33-42 (1999)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kimata-Ariga, Y., Hase, T.et al.: "Differential electron flow around photosystem I by two C_4-photosynthetic-cell-specific ferredoxins"EMBO J.. 19. 5041-5050 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakayama, M., Hase, T.et al.: "Plant sulfite reductase:molecular structure,catalytic function and interaction with ferredoxin"J.Inorg.Biochem.. 82. 27-32 (2000)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] Onda, Y., Hase, T.et al.: "Differential interaction of maize root ferredoxin:NADP^+ oxidoreductase with photosynthetic and non-photosynthetic ferredoxin isoproteins"Plant Physiol.. 123. 1037-1046 (2000)

    • Description
      「研究成果報告書概要(和文)」より
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      2001 Final Research Report Summary
  • [Publications] Yonekura-Sakakibara, K., Hase, T.et al.: "Analysis of reductant supply systems for ferredoxin dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize"Plant Physiol.. 122. 887-894 (2000)

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      2001 Final Research Report Summary
  • [Publications] Kurisu, G., Hase, T.et al.: "Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP^+ reductase"Nature Struct.Biol.. 8. 117-121 (2001)

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      2001 Final Research Report Summary
  • [Publications] Esaka, M., Nishimura, M.et al.: "cDNA cloning and differential gene expression of three catalases in pumpkin"Plant Mol.Biol.. 33. 141-155 (1997)

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      2001 Final Research Report Summary
  • [Publications] Hayashi, M., Nishimura, M.et al.: "Changes in targeting efficiencies of proteins to plant microbodies caused by amino acid substitution in the carboxy-terminal tripeptide"Plant Cell Physiol.. 38. 759-768 (1997)

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      2001 Final Research Report Summary
  • [Publications] Mano, S., Nishimura, M.et al.: "Hydroxypyruvate reductase with a carboxy-terminal targeting signal to microbodies is expressed in Arabidopsis"Plant Cell Physiol.. 38. 449-455 (1997)

    • Description
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      2001 Final Research Report Summary
  • [Publications] Mano, S., Nishimura, M.et al.: "Stromal and thylakoid-bound ascorbate peroxidases are produced by alternative splicing in pumpkin"FEBS Lett.. 413. 21-26 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, H., Nishimura, M.et al.: "Molecular characterization of a glyoxysomal long chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin"J.Biol.Chem.. 273. 8301-8307 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, H., Nishimura, M.et al.: "2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects in glyoxysomal fatty acid β-oxidation"Plant Cell. 10. 183-195 (1998)

    • Description
      「研究成果報告書概要(和文)」より
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      2001 Final Research Report Summary
  • [Publications] Kato, A., Nishimura, M.et al.: "Glyoxysomal malate dehydrogenase in pumpkin:cloning of a cDNA and functional analysis of its presequence"Plant Cell Physiol.. 39. 186-195 (1998)

    • Description
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      2001 Final Research Report Summary
  • [Publications] De Bellis, L., Nishimura, M.et al.: "Purification and characterization of pumpkin long-chain acyl-CoA oxidase"Physiol.Plant.. 106. 170-176 (1999)

    • Description
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      2001 Final Research Report Summary
  • [Publications] Hayashi, H., Nishimura, M.et al.: "A novel acetyl-CoA oxidase that can oxidize short-chain acyl-CoA in plant peroxisomes"J.Biol.Chem.. 274. 12715-12721 (1999)

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      2001 Final Research Report Summary
  • [Publications] Kato, A., Nishimura, M.et al.: "Oligomeric proteins containing N-terminal targeting signals are imported into peroxisomes in transgenic Arabidopsis"Plant Cell Physiol.. 40. 586-591 (1999)

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      2001 Final Research Report Summary
  • [Publications] Koumoto, Y., Nishimura, M.et al.: "Chloroplast Cpn20 forms a tetrameric structure in Arabidopsis thaliana"Plant J.. 17. 467-477 (1999)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, S., Nishimura, M.et al.: "Light regulates alternative splicing of hydroxypyruvate reductase in pumpkin"Plant J.. 17. 309-320 (1999)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] De Bellis, L., Nishimura, M.et al.: "Purification and characterization of a novel pumpkin short-chain acyl-Coenzyme A oxidase with structural similarity to acyl-Coenzyme A dehydrogenases"Plant Physiol.. 123. 327-334 (2000)

    • Description
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      2001 Final Research Report Summary
  • [Publications] Hayashi, M., Nishimura, M.et al.: "AtPex14p maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes"EMBO J.. 19. 5701-5710 (2000)

    • Description
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      2001 Final Research Report Summary
  • [Publications] Yamaguchi, K., Nishimura, M.: "Reduction to below threshold levels of glycolate oxidase activities in transgenic tobacco enhances photoinhibition during irradiation"Plant Cell Physiol.. 41. 1397-1406 (2000)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fukao, Y., Nishimura, M.et al.: "Developmental analysis of a putative ATP/ADP carrier protein localized on glyoxysomal membranes during the peroxisome transition in pumpkin"Plant Cell Physiol.. 42. 835-841 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koumoto, Y., Nishimura, M.et al.: "Chloroplasts have a novel Cpn10 in addition to Cpn20 as co-chaperonins in Arabidopsis thaliana"J.Biol.Chem.. 276. 29688-29694 (2001)

    • Description
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      2001 Final Research Report Summary
  • [Publications] Nito, K., Nishimura, M.et al.: "Pumpkin peroxisomal ascorbate peroxidase is localized on peroxisomal membranes and unknown membranous structures"Plant Cell Physiol.. 42. 20-27 (2001)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, Y., Nishimura, M.et al.: "Direct interaction between glyoxysomes and lipid bodies in cotyledons of the Arabidopsis thaliana ped1 mutant"Protoplasma. 218. 83-94 (2001)

    • Description
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    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, T., Murakami, S., Shoji, T., Yoshida, S., Yamada, Y., and Sato, F.: "A novel protein with DNA binding activity from tobacco chloroplast nucleoids."Plant Cell. 9. 1673-1682 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeda, S., Kaneko, Y., Matsushima, H., Yamada, Y., and Sato, F.: "Cultured green cells of tobacco as a useful material for the study of chloroplast replication."Methods Cell Sci.. 21. 149-154 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murakami, S., Kondo, Y., Nakano, T., and Sato, F.: "Protease activity of CND41, a chloroplast nucleoid DNA-binding protein, isolated from cultured tobacco cells."FEBS Lett.. 468. 15-18 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ifuku, K., and Sato, F.: "Importance of the N-terminal sequence of the extrinsic 23 kDa polypeptide in photosystem II in ion retention in oxygen evolution."Biochim. Biophys. Acta. 1546. 196-204 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugita, M., Svab, Z., Maliga, P., and Sugiura, M.: "Targeted deletion of sprA from the tobacco plastid genome indicates that the encoding small RNA is not essential for pre-16S rRNA maturation in plastids."Mol. Gen. Genet.. 257. 23-27 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyagai, T., Kapoor, S., Sugita, M., and Sugiura, M.: "Transcript analysis of the tobacco plastid operon rps2/atpI/H/F/A reveals the existence of a non-consensus type II (NCII) promoter upstream of the atpI coding sequence."Mol. Gen. Genet.. 257. 299-307 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mutsuda, M., Sugiura, M., and Sugita, M.: "Physiological characterization of RNA-binding protein-deficient cells from Synechococcus sp. strain PCC7942."Plant Cell Physiol.. 40. 1203-1209 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, T., Ohta, M., Sugiura, M., and Sugita, M.: "Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs."FEBS Lett.. 460. 437-441 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugita, C., Sugiura, M., and Sugita, M.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp. PCC6301 : a soluble 33-kDa polypeptide with high sequence similarity to ribosomal protein S1."Mol. Gen. Genet.. 263. 655-663 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, T., Ohta, M., Sugiura, M., and Sugita, M.: "Chloroplast ribonucleoproteins function as a stabilizing factor of ribosome-free mRNAs in the stroma."J. Biol. Chem.. 276. 147-152 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, Y., Dokiya, Y. and Sugita, M.: "Dual targeting of phage-type RNA polymerase to both mitochondria and plastids is due to alternative translation initiation in single transcripts."Biochem. Biophys. Res. Commun.. 289. 1106-1113 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, Y., Dokiya, Y., Sugiura, M., Niwa, Y., and Sugita, M.: "Genomic organization and organ-specific expression of a nuclear gene encoding phage-type RNA polymerase in Nicotiana sylvestris."Gene. 279. 33-40 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aichi, M., and Omata, T.: "Involvement of NtcB, a LysR family transcription factor, in nitrite activation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp. strain PCC 7942."J. Bacteriol.. 179. 4671-4675 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harano, Y., Suzuki, I., Maeda, S., Kaneko, T., Tabata, S., and Omata, T.: "Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942."J. Bacteriol.. 179. 5744-5750 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, M., Rodriguez, R., Lara, C., and Omata, T.: "Involvement of the C-terminal domain of an ATP-binding subunit in the regulation of the ABC-type nitrate/nitrite transporter of the cyanobacterium Synechococcus sp. strain PCC 7942."J. Biol. Chem.. 272. 27197-27201 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, S., Kawaguchi, Y., Ohe, T., and Omata, T.: "cis -Acting sequences required for NtcB-dependent, nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp. strain PCC 7942."J. Bacteriol.. 180. 4080-4088 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, S., Okamura, M., Kobayashi, M., and Omata, T.: "Nitrite-specific active transport system of the cyanobacterium Synechococcus sp. strain PCC 7942."J. Bacteriol.. 180. 6761-6763 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Rodriguez, R., Kobayashi, M., Omata, T., and Lara, C.: "Independence of carbon and nitrogen control in the posttranslational regulation of nitrate transport in the cyanobacterium Synechococcus sp. strain PCC 7942."FEBS Lett.. 432. 207-212 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Omata, T., Price, G. D., Badger, M. R., Okamura, M., Gohta, S., and Ogawa, T.: "Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942."Proc. Natl. Acad. Sci. USA.. 96. 13571-13576 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, S., Price, G. D., Badger, M. R., Enomoto, C., and Omata, T.: "Bicarbonate binding activity of the CmpA protein of the cyanobacterium Synechococcus sp. strain PCC 7942 involved in active transport of bicarbonate."J. Biol. Chem.. 275. 20551-20555 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Omata, T., Gohta, S., Takahashi, Y., Harano, Y., and Maeda, S.: "Involvement of a CbbR homolog in low CO_2 induced activation of the bicarbonate transporter operon in cyanobacteria."J. Bacteriol.. 183. 1891-1898 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aichi, M., Takatani, N., and Omata, T.: "Role of NtcB in activation of the nitrate assimilation genes in the cyanobacterium Synechocystis sp. strain PCC 6803."J. Bacteriol.. 183. 5840-5847 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumura, T., Sakakibara, H., Nakano, R., Kimata, Y., Sugiyama, T., and Hase, T.: "A nitrate-inducible ferredoxin in maize roots : genomic organization and differential expression of two nonphotosynthetic ferredoxin isoproteins."Plant Physiol.. 114. 653-660 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, R., Matsumura, T., Sakakibara, H., Sugiyama, T., and Hase, T.: "Cloning of maize ferredoxin III gene : presence of a unique repetitive nucleotide sequence within an intron found in the 5'-untranslated region."Plant Cell Physiol.. 38. 1167-1170 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taniguchi, I., Miyahara, A., Iwakiri, K., Hirakawa, K., Hayashi, K., Nishiyama, K., Akashi, T., and Hase, T.: "Electrochemical study of biological functions of particular evolutionary conserved amino acid residues using mutated molecules of maize ferredoxin."Chem. Lett.. 1997. 929-930 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yonekura-Sakakibara, K., Ashikari, T., Tanaka, Y., Kusumi, T., and Hase, T.: "Molecular characterization of tobacco sulfite reductase : enzyme purification, gene cloning and gene expression analysis."J. Biochem.. 124. 615-621 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akashi, T., Matsumura, T., Ideguchi, T., Iwakiri, K., Kawakatsu, T., Taniguchi, I., and Hase, T.: "Comparison of the electrostatic binding sites on the surface of ferredoxin for two ferredoxin-dependent enzymes, ferredoxin- NADP+ oxidoreductase and sulfite reductase."J. Biol. Chem.. 274. 29399-29405 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumura, T., Kimata-Ariga, Y., Sakakibrra, H., Sugiyama, T., Murata, H., Takao, T., Shimonishi, Y., and Hase, T.: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves."Plant Physiol.. 199. 491-498 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okuhara, H., Matsumura, T., Fujita, Y., and Hase, T.: "Cloning and inactivation of genes encoding ferredoxin- and NADH-dependent glutamate synthase in the cyanobacterium Plectonema boryanum : Imbalances in nitrogen and carbon assimilations caused by deficiency of the ferredoxin-dependent enzyme."Plant Physiol.. 120. 33-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kimata-Ariga, Y., Matsumura, T., Kada, S., Fujimoto, H., Fujita, Y., Endo, T., Mano, J., Sato, F., and Hase, T.: "Differential electron flow around photosystem I by two C4-photosynthetic-cell-specific ferredoxins."EMBO J.. 19. 5041-5050 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakayama, M., Akashi, T., and Hase, T.: "Plant sulfite reductase : molecular structure, catalytic function and interaction with ferredoxin."J. Inorg. Biochem.. 82. 27-32 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Onda, Y., Matsumura, T., Kimata-Ariga, Y., Sakakibara, H., Sugiyama, T., and Hase, T.: "Differential interaction of maize root ferredoxin : NADP+ oxidoreductase with photosynthetic and non-photosynthetic ferredoxin isoproteins."Plant Physiol.. 123. 1037-1045 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yonekura-Sakakibara, K., Onda, Y., Ashikari, T., Tanaka, Y., Kusumi, T., and Hase, T.: "Analysis of reductant supply systems for ferredoxin dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of"Plant Physiol.. 122. 887-894 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kurisu, G., Kusunoki, M., Katoh, E., Yamazaki, T., Teshima, K., Onda, Y., Kimata-Ariga, Y., and Hase, T.: "Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP+ reductase."Nature Struct. Biol.. 8. 117-121 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Esaka, M., Yamada, N., Kitabayashi, M., Setoguchi, Y., Tsugeki, R., Kondo, M., and Nishimura, M.: "cDNA cloning and differential gene expression of three catalases in pumpkin."Plant Mol. Biol.. 33. 141-155 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, M., Aoki, M., Kondo, M., and Nishimura, M.: "Changes in targeting efficiencies of proteins to plant microbodies caused by amino acid substitution in the carboxy-terminal tripeptide."Plant Cell Physiol.. 38. 759-768 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, S., Hayashi, M., Kondo, M., and Nishimura, M.: "Hydroxypyruvate reductase with a carboxy-terminal targeting signal to microbodies is expressed in Arabidopsis."Plant Cell Physiol.. 38. 449-455 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, S., Yamaguchi, K., Hayashi, M., and Nishimura, M.: "Stromal and thylakoid-bound ascorbate peroxidases are produced by alternative splicing in pumpkin."FEBS Lett.. 413. 21-26 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, H., De Bellis, L., Yamaguchi, K., Kato, A., Hayashi, M., and Nishimura, M.: "Molecular characterization of a glyoxysomal long chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin."J. Biol. Chem.. 273. 8301-8307 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, M., Toriyama, K., Kondo, M., and Nishimura, M.: "2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects in glyoxysomal fatty acid b-oxidation."Plant Cell. 10. 183-195 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kato, A., Takeda-Yoshikawa, Y., Hayashi, M., Kondo, M., Hara-Nishimura, I., and Nishimura, M.: "Glyoxysomal malate dehydrogenase in pumpkin : cloning of a cDNA and functional analysis of its presequence."Plant Cell Physiol.. 39. 186-195 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] De Bellis, L., Giuntini, P., Hayashi, H., Hayashi, M., and Nishimura, M.: "Purification and characterization of pumpkin long-chain acyl-CoA oxidase."Physiol. Plant.. 106. 170-176 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, H., De Bellis, L., Ciurli, A., Kondo, M., Hayashi, M., and Nishimura, M.: "A novel acetyl-CoA oxidase that can oxidize short-chain acyl-CoA in plant peroxisomes."J. Biol. Chem.. 274. 12715-12721 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kato, A., Hayashi, M., and Nishimura, M.: "Oligomeric proteins containing N-terminal targeting signals are imported into peroxisomes in transgenic Arabidopsis."Plant Cell Physiol.. 40. 586-591 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koumoto, Y., Shimada, T., Kondo, M., Takao, T., Shimonishi, Y., Hara-Nishimura, I., and Nishimura, M.: "Chloroplast Cpn20 forms a tetrameric structure in Arabidopsis thaliana."Plant J.. 17. 467-477 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, S., Hayashi, M., and Nishimura, M.: "Light regulates alternative splicing of hydroxypyruvate reductase in pumpkin."Plant J.. 17. 309-320 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murakami,S.,& Sato,F. et al.: "Protease activity of CND41, a chloroplast nucleoid DNA-binding protein, isolated from cultured tobacco cells."FEBS Lett.. 468. 15-18 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitajima,S.,& Sato,F. et al.: "Characterization of gene expression of ERFs, transcription factors of basic PR genes from Nicotiana sylvestris."Plant Cell Physiol.. 41(1). 817-824 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato,F. et al.: "Regulation of PR-5d (osmotin-like protein) gene expression via ERFs (ethylene responsive factors)."2000 Japan-Korea Joint Symposium. Vol.14. 103-109 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukushima,E.& Sato,F. et al.: "Improved salt tolerance of transgenic tobacco expressing apoplastic yeast-derived invertase."Plant Cell Physiol.. 42(2). 245-249 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ifuku K.,& Sato,F.: "N-terminal sequence of the extrinsic 23 kDa protein in photosynstem II has importance in ion-retention in oxygen-evolution."Biochim.Biophys.Acta. 1546/1. 196-204 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koyama,T.& Sato,F. et al.: "Expression of PR-5d and ERF genes in cultured tobacco cells and their NaCl stress-response."Biosci.Biotech.Biochem.. 65(6)(in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Omata,T., et al.: "Involvement of a CbbR homolog in low CO2-induced activation of the bicarbonate transporter operon in cyanobacteria."J.Bacteriol. 183(6). 1891-1898 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yonekura-Sakakibara K.,& Hase T. et al.: "Analysis of reductant supply systems for ferredoxin dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize."Plant Physiol.. 122. 887-894 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Onda Y.,& Hase T. et al.: "Differential interaction of maize root ferredoxin-NADP+ oxidoreductase with photosynthetic and nonphotosynthetic ferredoxin isoproteins."Plant Physiol.. 123. 1037-1045 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kimata-Ariga,Y.,Sato,F.,& Hase,T.: "Differential electron flow around photosystem I by two C4-photosynthetic-cell-specific ferredoxins."EMBO J.. 19. 5041-5050 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakayama,M.,& Hase,T.: "Plant sulfite reductase : molecular structure, catalytic function and interaction with ferredoxin."J.Inorg.Biochem.. 82. 27-32 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato,N.,& Hase,T. et al.: "The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase."FEBS Lett.. 487. 247-350 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 佐藤文彦: "光独立栄養培養細胞株を用いた植物機能の分子細胞生物学的解析とその応用"植物の化学調節. 35(1). 8-16 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugita,C.,& Sugita,M. et al.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp.PCC6301 : a 33 kDa soluble polypeptide with high sequence similarity to ribosomal protein S1."Mol.Gen.Genet.. 263. 655-663 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yukawa,Y.,Sugita,M., et al.: "The TATA motif, the CAA motif and the poly (T) transcription termination motif are all important for transcription re-initiation on plant tRNA genes."The Plant J.. 22. 439-447 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakamura,T.,& Sugita,M. et al.: "Chloroplast ribonucleoproteins function as a stabilizing factor of ribosome-free mRNAs in the stroma."J.Biol.Chem.. 276. 147-152 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hara,K.,Sugita,M., et al.: "Cloning and characterization of the cDNA for a plastid sigma factor from the moss Physcomitrella patens."Biochim.Biophys.Acta. 1517. 302-306 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Maeda,S.,& Omata,T. et al.: "Bicarbonate-binding activity of the CmpA protein of the cyanobacterium Synechococcus sp.strain PCC 7942 involved in active transport of bicarbonate."J.Biol.Chem.. 275(27). 20551-20555 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kurisu,G.,& Hase,T.: "Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP+ reductase."Nature Struct.Biol.. 8. 117-121 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Minami,Y.,& Nishimura,M. et al.: "Tissue and intracellular localization of indican and the purification and characterization of indican synthase from Indigo plants"Plant Cell Physiol.. 41. 218-225 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takemoto,D.,Nishimura,M. et al.: "Isolation of gene for EIR, an elicitor inducible receptor like protein, from tobacco by differential display."Plant Cell Physiol.. 41. 458-464 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] De Bellis,L.,& Nishimura,M. et al.: "Purification and characterization of a novel pumpkin short-chain acyl-CoA oxidase which structure resembles acyl-CoA dehydrogenase."Plant Physiol.. 123. 327-334 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mitsuhashi,N.,& Nishimura,M. et al.: "Characterization of organelles in the vacuolar-sorting pathway by -visualization with GFP in tobacco BY-2 cells."Plant Cell Physiol.. 41. 993-1001 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayashi,M.,& Nishimura,M. et al.: "AtPex14 maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes."EMBO J.. 19. 5701-5710 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamaguchi,K.& Nishimura,M.: "Reduction to below threshold levels of glycolate oxidase activities in transgenic tobacco enhances photoinhibition during irradiation."Plant Cell Physiol.. 41. 1397-1406 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nito,K.,& Nishimura,M. et al.: "Pumpkin peroxisomal ascorbate peroxidase is localized on peroxisomal membranes and unknown membranous structures."Plant Cell Physiol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayashi-Ishimaru,Y.,& Nishimura,M. et al.: "Glyoxysome-spherosome interaction in etiolated cotyledons of Arabidopsis Ped1 mutant that has defect on a thiolase gene of fatty acid b-oxidation cycle."Protoplasma. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Mano,S.,& Nishimura,M. et al.: "A leaf-peroxisomal protein, hydroxypyruvate reductase, is produced by light-regulated alternative splicing."Cell Biochem.Biophys.. 32. 147-154 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kato,A.,& Nishimura,M. et al.: "Transport of peroxisomal proteins that are synthesized as large precursors in plants."Cell Biochem.Biophys.. 32. 269-275 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayashi,M.,& Nishimura,M.: "Functional transformation of plant peroxisomes"Cell Biochem.Biophys.. 32. 295-304 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeda, S.: "Cultured green cells of tobacco as a useful material for the study of chloroplast replication"Methods in Cell Sciences. 21. 149-154 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Endo, T.: "The role of chlorplastic NAD(P)H dehy drogenase in photoprotection"FEBS Lett.. 457. 5-8 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ifuku, K.: "Isolation of cucumber cDNA (accession No. AB03225) for the 23kDa polypeptide of the oxygen evolving complex of photosystem II (psbP) (PGR00-031)"Plant Physiol.. 122. 619 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murakami, S.: "Protease activity of CND41, a chloroplast nucleoid DNA binding protein isolated from cultured tobacco cells"FEBS Lett.. 468. 15-18 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato, F.: "CND41, a DNA binding protein in chloroplast nucleoid, and its function"In "The 13th Symposium on plant Biotechnology ; 1999 Korea Japan Joint Symposium". 13. 51-56 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 佐藤文彦: "葉緑体機能と個体統御"蛋白質核酸酵素. 45. 147-152 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 佐藤文彦: "葉緑体構築のダイナミックス 序説"蛋白質核酸酵素. 45. 105-107 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugita, C.: "Targeted deletion of genes for eukaryotic RNA binding proteins, Rbp1 and Rbp2, in cyanobacterium Synechococcus sp. strain PCC7942 : Rbp1 gene is indispensable for cell growth at low temperatures"FEMS Microbiol. Lett.. 176. 142-147 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakamura, T.: "Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs"FEBS Lett.. 460. 437-441 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mutsuda, M.: "Physiological characterization of RNA-binding protein ?deficient cells from Synechococcus sp. strain PCC7942"Plant Cell Physiol.. 40. 1203-1209 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugita, C.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp. PCC6301 : a 33 kDa soluble polypeptide with high sequence similarity to ribosomal protein S1."Mol. Gen. Genet.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 杉田護: "遺伝子遺伝子発現における転写後調節"蛋白質 核酸 酵素. 45. 132-138 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Omata, T.: "Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942"Proc. Natl. Acad. Sci. USA. 96(23). 13571-13576 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akashi, T.: "Comparison of the electrostatic binding sit es on the surface of ferredoxin for two ferredoxin-dependent enzymes, ferredoxin-NADP+ reductase and sulfite reductase"J. Biol. Chem.. 274. 29399-29405 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumura, T.: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves"Plant Physiol.. 119. 481-488 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Okuhara, H.: "Cloning and inactive ation of genes encoding ferredoxin and NADH-dependent glutamate synthases in the cyanobacterium Plectonema boryanum. Imbalances in nitrogen and carbon assimilations caused by deficiency of the ferredoxin dependent enzymes"Plant Physiol.. 120. 33-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yonekura-Sakakibara, K.: "Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize"Plant Physiol.. 122. 887-894 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamada, K.: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor protein by vacuolar processing enzyme"J. Biol. Chem.. 274. 2563-2570 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hayashi, M.: "Induction of precursor-accumulating vesicles by expression of chimeric genes consisting of pumpkin 2S albumin and phosphinothricin acetyltransferase"Plant Cell Physiol.. 40. 263-272 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mano, S.: "Light regulates alternative splicing of hydoroxypyru vate reductase in pumpkin"Plant J.. 17. 309-320 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Koumoto, Y.: "Chloroplast Cpn 20 forms a tetrameric structure in Arabidopsis thaliana"Plant J.. 17. 467-477 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiraiwa, N.: "Vacuolar processing enzyme is activated self-catalytically by sequential removal of the C-terminal and N-terminal propeptides"FEBS Lett.. 447. 213-216 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hayashi, H.: "A novel oxidase that catalyzes oxidation of short-chain acyl CoA in plant peroxisomes"J. Biol. Chem.. 274. 12715-12721 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kato, A.: "Oligomeric proteins containing N-terminal targeting signals are imported into peroxisomes in transgenic Arabidopsis"Plant Cell Physiol.. 40. 586-591 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kinoshita, T.: "Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescence and under various stressed conditions"Plant J.. 19. 43-53 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] De Bellis L.: "Purification and characterization of pumpkin long-chain acyl-CoA oxidase"Plant Physiol.. 106. 170-176 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takemoto, D.: "Molecular cloning of a defense-response-related cytochrome P450 gene from tobacco using a fungal elicitor"Plant Cell Physiol.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] De Bellis, L.: "Purification and characterization of a novel pumpkin short-chain acyl-CoA oxidase which structure resembles acyl-CoA dehydrogenase"Plant Physiol.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitajima,S.: "Constitutive expression of the neutral PR-5 (OLP,PR-5d) gene in roots and cultured cells of tobacco is mediated by ethylene-responsive cis-element AGCCGCC sequences." Plant Cell Reports. 18. 173-179 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo,T.: "Inhibition of photosystem II of spinach by lichen-derived depsides." Biosci.Biotech.Biochem.62. 2023-2027 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo,T: "NAD(P)H dehydrogenase-dependent, antimycin a-sensitive electron donation to plastoquinone in tobacco chloroplasts." Plant Cell Physiol.39. 1226-1231 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitajima,S.: "Plant pathogeniesis-related proteins: molecular mechanisms of gene expression and protein function." J.Biochem.125. 1-8 (1991)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ifuku,K.: "Reconstitution of photosynthetic oxygen evolving activity with heterologous OEC23 protein expressed in E.coli." Research in Photosynthesis, Kluwer Acad.Pub. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Murakami,S: "Purification of CND41: chloroplast nucleoid DNA-binding protein with proteolytic activity." Research in Photosynthesis, Kluwer Acad.Pub. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyagi,T.: "Transcript analysis of tobacco plastid rps2/atp1/H/F/A operon reveals the existence of a non-consensus type II (NCII) promoter upstream to atpI coding sequence." Mol.Gen.Genet. 257. 299-307 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wakasugi,T: "Update gene map of tobacco chloroplast DNA" Plant Mol.Biol.Rep.16. 231-241 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugiura,M: "Evolution and mechanism of translation in chloroplasts" Annu.Rev.Genet.32. 437-459 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujita,Y: "Cloning of the gene encoding a protochlorophyllide reductase: the physiological significance of the coexistence of light-dependent and-independent protochlorophyllide reduction systems in the cyanobacterium Plectoncrn boryanum." Plant Cell Physiol.39. 177-185 (1998)

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      1998 Annual Research Report
  • [Publications] Yonekura-Sakakibara,K: "Molecular characterization of tobacco sulfite reductase:enzyme purification, gene cloning, and gene expression analysis." J.Biochem.124. 615-621 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okuhara,H: "Fd-GOGAT is necessary for the integration of photosynthetic C- and N- assimilation in cyanobacerium." Proc.XI International Photosynthesis Congress. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumura,T: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves." Plant Physiol.199. 481-488 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maeda,S: "cis-Acting sequences required for NtcB-dependent, nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium Synechocystis sp.strain PCC 7942" J.Bacteriol.180. 4080-4088 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Rodriguez,R: "Independence of carbon and nitrogen control in the posttranslational regulation of nitrate transport in the cyanobacterium Synechococcus sp.strain PCC 7942." FEBS Lett.432. 207-212 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maeda,S: "Nitrite-specific active transport system of the cyanobacterium Synechococcus sp.strain PCC7942." J.Bacteriol.180. 6761-6763 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kato,A: "Glyoxysomal malate dehydrogenase in pumpkin: cloning of a cDNA and functional analysis of its presequence." Plant Cell Physiol.39. 186-195 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayashi,M.: "2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects on glyoxysomal b-oxidation." Plant Cell. 10. 183-195 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hara-Nishimura,I: "Vacuolar processing enzymes in protein-storage vacuoles and lytic vacuoles" J.Plant Physiol.152. 668-674 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayashi,M: "Functional transformation of plant peroxisomes." Cell Biochem.Biophys.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayashi,H: "Molecular characerization of a glyoxysomal long-chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin." J.Biol.Chem.273. 8301-8307 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hara-Nishimura,I: "Transport of storage proteins to protein-strage vacuoles is mediated by large precursor-accumulating vesicles." Plant Cell. 10. 825-836 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamada,K: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor protein by vacuolar processing enzyme." J.Biol.Chem.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Ali,K.: "Levels of nonstructural carbohydrate in leaves and roots and some characteristics of chloroplasts after application of different amounts of nitrogen fertilizer to peach seedlings." J.Jap.Soc.Hort.Sci. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayashi,M: "Induction of precursor-accumulating vesicles by expression of chimeric genes consisting of pumpkin 2S albumin and phosphinothricin acetyltransferase." Plant Cell Physiol.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Mano,S: "Light regulates alternative splicing of hydroxypyruvate reductase in pumpkin" Plant J.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Koumoto,Y: "Chloroplast Cpn20 forms a tertrameric structure in Arabidopsis thaliana" Plant J.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishimura,M: "Molecular chaperones and temperature stress" Stress Responses of Photosynthesis Organisms. (Elsevier). 83-92 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishimura,M: "Microbody defective mutants of Arabidopsis" J.Plant Res.111. 329-332 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kato,A: "Transport peroxidomal prteins that are synthesized as large precurosors in plants." Cell Biochem.Biophy.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Nakano: "A novel protein with DNA binding activity from tobacco chloroplast nucleoids." Plant Cell. 9. 1673-1682 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐藤文彦: "葉緑体DNA結合タンパク質" 日本農芸化学会誌. 71. 1173-1176 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Miyagi: "Transcript analysis of tobacc o plastid rps2/atpI/H/F/A operon reveals the existence of a non-consensus type II (NCII) promoter upstream to atpI coding sequence." Mol.Gen.Genet.257・3. 299-307 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Sugita: "Targeted deletion of sprA from the tobacco genomeindicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids." Mol.Gen.Genet.257・1. 23-27 (1997)

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      1997 Annual Research Report
  • [Publications] Y.Yukawa: "Efficient in vitro transcription of plant nuclear tRNASer genes in a nuclear extract from tobacco cultured cells." Plant J.12・4. 965-970 (1997)

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      1997 Annual Research Report
  • [Publications] 杉田護: "葉緑体RNA結合蛋白質" 日本農芸化学会誌. 71. 1177-1179 (1997)

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      1997 Annual Research Report
  • [Publications] T.Matsumura: "A nitrate inducible ferredoxin in maize roots : genomic organization and differential expression of two nonphotosynthetic ferredoxin isoproteins." Plant Physiol.114. 653-660 (1997)

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      1997 Annual Research Report
  • [Publications] I.Taniguchi: "Electrochemical study of biological functions of particular evolutionary conserved amino acid residures using mutated molecules of maize ferredoxin." Chem.Lett.929-930 (1997)

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      1997 Annual Research Report
  • [Publications] R.Nakano,: "Cloning of maize ferredoxin III gene : presence of a unique repetitive nucleotide sequence within an intron in the 5'-untranslated region." Plant Cell Physiol.38. 1167-1170 (1997)

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      1997 Annual Research Report
  • [Publications] Y.Harano: "Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942." J.Bacteriol.179. 5744-5750 (1997)

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      1997 Annual Research Report
  • [Publications] M.Aichi: "Involvement of NtcB,a LysR family transcription factor,in nitrite activation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp. strain PCC 7942." J.Bacteriol.179. 4671-4675 (1997)

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      1997 Annual Research Report
  • [Publications] M.Kobayashi: "Involvement of the C-terminal domain of an ATP-binding subunit in the regulation of the ABC-type nitrate/nitrite transporter of the cyanobacterium Synechococcus sp. strain PCC 7942." J.Biol.Chem.272. 27197-27201 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Omata: "Transcriptional and post-translational regulation of nitrate utilization in the cyanobacterium Synechococcus sp. strain PCC 7942." In Stress Response of Photosynthetic Organisms. 197-214 (1997)

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      1997 Annual Research Report
  • [Publications] M.Esaka: "cDNA cloning and differential gene expression of three catalases in pumpkin." Plant Mol.Biol.33. 141-155 (1997)

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      1997 Annual Research Report
  • [Publications] S.Mano: "Hydroxypyruvate reductase with a carboxy-terminal targeting signal to microbodies is expressed in Arabidopsis." Plant Cell Physiol.38. 449-455 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Hayashi: "Changes in targeting efficiencies of proteins to plant microbodies caused by amino acid substitution in the carboxy-terminal tripeptide." Plant Cell Physiol.38. 759-768 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Mano: "Stromal and thylakoid-bound ascorbate peroxidases are produced by alternative splicing in pumpkin." FEBS Lett.413. 21-26 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Kato: "Glyoxysomal malate dehydrogenase in pumpkin : cloning of a cDNA and functional analysis of its presequence." Plant Cell Physiol.(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Hayashi: "2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects on gloxysomal β-oxidation." Plant Cell. (in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Hayashi: "Molecular characterization of a glyoxysomal long-chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin." J.Biol.Chem.(in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Nishimura: "Molecular chaperones and temperature stress. In Stress Responses of Photosynthesis Organisms :" Molecular Mechanisms and Molecular Regulations. 83-42 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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