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Dynamic interaction between chaperone and its substrates

Research Project

Project/Area Number 09276101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YOSHIDA Masasuke  Chemical Resources Laboratory Tokyo Institute of Technology, Prof., 資源化学研究所, 教授 (90049073)

Co-Investigator(Kenkyū-buntansha) GOTO Yuji  Osaka Univ. Prof., 蛋白質研究所, 教授 (40153770)
KOHNO Kenji  Nara Ist. Sci. Tech. Prof., 遺伝子教育センター, 教授 (50142005)
KUWAJIMA Kunihiro  Tokyo Univ. Prof., 大学院・理学系研究科, 教授 (70091444)
FUNATSU Takashi  Waseda Univ. Prof., 理工学部, 助教授 (00190124)
TOKUDA Gen  Tokyo Univ. Prof., 分子細胞生物学研究所, 教授 (40125943)
南 康文  大分医科大学, 医学部, 助教授 (40181953)
河田 康志  鳥取大学, 工学部, 助教授 (40177697)
石川 統  東京大学, 大学院・理学系研究科, 教授 (70012482)
Project Period (FY) 1997 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥296,000,000 (Direct Cost: ¥296,000,000)
Fiscal Year 2000: ¥69,000,000 (Direct Cost: ¥69,000,000)
Fiscal Year 1999: ¥79,000,000 (Direct Cost: ¥79,000,000)
Fiscal Year 1998: ¥61,000,000 (Direct Cost: ¥61,000,000)
Fiscal Year 1997: ¥87,000,000 (Direct Cost: ¥87,000,000)
KeywordsMolecular chaperone, / folding, / stress, / stress protein, / heat-shock protein, / chaperonin, / protein secretion, / purion / 熱ショックタンパク質 / GroEL / 蛋白質の膜透過 / タンパク質 / Hsp90 / タンパク質の膜透過 / インクルージョンボディ
Research Abstract

Summary
Chaperonin encapsulates unfolded proteins in the cis cavity and ensures safe folding without fear of aggregation. This process requires the energy of ATP hydrolysis. Currently prevailing model of chaperonin function assumes only a single rate-limiting step in whole catalytic cycle of chaperonin, that is, ATP hydrolysis that needs 8 sec. However, Yoshida and Funatsu〓s group have proposed that there are two rate limiting steps in the cycle, one 3 sec and the other 5 sec. This is based on the single molecule observation that GroES release, ADP release, and protein folding initiated by addition of ATP only start after a 3-sec lag phase. Kuwajima showed the conformational change occurred by the ATP binding rather than ATP hydrolysis. Tokuda〓 group have found a new factor, SecG, in SecA system. Similar to SecA, during protein translocation, SecG tumbles in the membrane. Kohno identified that ER stress sensor protein, IRE1-beta, is an RNase that cleaves 28SrRNA. Gotoh succeeded in expression of human beta-microglobulin in yeast cells and pointed out that disulfide bond is important in formation of amyloid fibers. Yoshida〓s group has established a real-time observation system that allows the growth of single amyloid fiber. The result showed that fiber grew predominantly unnidirectionally.

Report

(5 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] Aoki K, Motojima F, Taguchi H, Yomo T, Yoshida M.: "GroEL binds artificial proteins with random sequences"J Biol Chem. 275. 13755-13758 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fukuda H, Arai M, Kuwajima K: "Folding of green fluorescent protein and the cycle3 mutant"Biochemistry.. 39. 12025-32 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwawaki T, Hosoda A, Okuda T, Kamigori Y, Nomura-Furuwatari C, Kimata Y, Tsuru A, Kohno K.: "Translational control by ER transmembrane kinase/ribonuclease IRE1 under ER stress"Nat Cell Biol. 3. 158-164 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuwata K, Shastry R, Cheng H, Hoshino M, Batt CA, Goto Y, Roder H.: "Structural and kinetic characterization of early folding events in beta-lactoglobulin"Nat Struct Biol. 8. 151-155 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuwata K, Li H, Yamada H, Batt CA, Goto Y, Akasaka K.: "High pressure NMR reveals a variety of fluctuating conformers in beta-lactoglobulin"J Mol Biol.. 305. 1073-83 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yakushi T, Masuda K, Narita S, Matsuyama S, Tokuda H: "A new ABC transporter mediating the detachment of lipid-modified proteins from membranes"Nat Cell Biol. 2. 212-218 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 永田和宏, 森正敬, 吉田賢右: "分子シャペロンによる細胞機能制御"シュプリンガー・フェアラーク東京. 219 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aoki K, Motojima F, Taguchi H, Yomo T, Yoshida M.: "GroEL binds artificial proteins with random sequences."J Biol Chem.. 275. 13755-8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fukuda H, Arai M, Kuwajima K.: "Folding of green fluorescent protein and the cycle3 mutant."Biochemistry.. 39. 12025-32 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwawaki T, Hosoda A, Okuda T, Kamigori Y, Nomura-Furuwatari C, Kimata Y, Tsuru A, Kohno K.: "Translational control by ER transmembrane kinase/ribonuclease IREI under ER stress."Nat Cell Biol. 3. 158-64 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuwata K, Shastry R, Cheng H, Hoshino M, Batt CA, Goto Y, Roder H.: "Structural and kinetic characterization of early folding events in beta-lactoglobulin."Nat Struct Biol. 8. 151-5 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuwata K, Li H, Yamada H, Batt CA, Goto Y, Akasaka K.: "High pressure NMR reveals a variety of fluctuating conformers in beta-lactoglobulin."J Mol Biol.. 305. 1073-83 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yakushi T, Masuda K, Narita S, Matsuyama S, Tokuda H.: "A new ABC transporter mediating the detachment of lipid-modified proteins from membranes."Nat Cell Biol. 2. 212-8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eds.Nagata,K.,Mori,M.,Yoshida,M.: "Regulation of cellular function by molecular chaperones."Springer-Verlag Tokyo. 219 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aoki,K.: "GroEL binds artificial proteins with random sequences."J.Biol.Chem.. 275. 13755-13758 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Watanabe,Y.: "Heat-inactivated proteins managed by DnaKJ-GrpE-ClpB chaperones are released as a chaperonin-recongnizable nonnative form."J.Biol.Chem.. 275. 12388-12392 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Iwawaki,T.: "Translational control by ER transmembrane kinase/ribonuclease IRE1 under ER stress."Nature Cell Biol.. 3. 158-164 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kuwata,K.: "Structural and kinetic characterization of early folding events in beta-lactoglobulin."Nature Struct.Biol.. 8. 151-155 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yakushi,T.: "A new ABC transportec mediating the detachment of lipid-modified proteins from membranes."Nature Cell Biol.. 2. 212-218 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mizobata,T.: "Refolding of target proteins from a "rigid" mutant chaperonin demonstrates a minimal mechanism of chaperonin binding and release."J.Biol.Chem.. 275. 25600-25607 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田口英樹(分担): "タンパク質のケアテイカー、分子シャペロン"共立出版. 200 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 後藤祐児(編集): "タンパク質の分子設計"共立出版. 200 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakikawa, C.: "On the maximum size of proteins to stay and fold in GroEL underhealth GroES"J. Biol. Chem.. 274. 21251-21256 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Minami, Y.: "Hsc70/Hsp40 chaperone system mediates the Hsp90 dependent folding of luciferase"Genes to Cells. 4. 721-729 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamasaki, R.: "Single molecular observation of the interaction of GroEL with substrate protein"J. Mol. Biol.. 292. 965-972 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishiyama, K.: "Membrane-deinsertion of Sec A underlying proton motive-force dependent stimulation of protein translocation"EMBO J.. 18. 1049-1058 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Asano, T.: "Selective association of G Protein b4 with gamma 5 and gamma 12 subunits in Borine tissues"J. Biol. Chem.. 274. 21425-21429 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Harada, Y.: "Single molecule imaging of RNA polymerase-DNA interactions in real time"Biophysical J.. 76. 709-715 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 船津高志: "生命科学を拓く新しい光技術"共立出版. 186 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taguchi,H.: "Chaperonin from thermephile Thermus thernophilus" Methods Enzymol.290. 169-180 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yahara,I.: "The 90-kDn Stress Protein,Hsp90,Isa Novel Molecular Chaperone" Ann.N.Y.Aca.Sci.851. 54-60 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishiyama,K.: "Membrane-deinsertion of SccA underlying protein mutive force-dependent stimulation of protein translocation" EMBOJ. 18(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Morioka,M.: "Insect chaperonin 60:symbionin" Methods in Fnzymology. 290. 181-193 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Machida,S.: "Owrproduction of β-Glucosidass in Active form by an Exchimchin coli systim caexpressing the chaperenin GroFI/ES" FEMS Microbiology Letters. 159. 41-46 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kumata,K.: "Alpha→beta transition of beta-lactoglobulin as evidenced by heteronuclear" J.Mol.Biol.283. 731-739 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsurupa,G.P.: "Refolding kinetics of staphyloco ccal nucliase and its mutant in the presence of the chaperonin GroFL" J Mol Biol. 277(3). 733-45 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Aoki, K.: "Colorimetric observation of a GroEL-Protein binding reaction with little Contribution of hydrophobic interaction" J. Biol, Chem.272. 32158-32162 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Machida, S.: "Overproduction of β-glucosidase in active form by an Escherichia coli System coexpressing the chaperohin GroEL/ES" FEMS Lett. 159. 41-46 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hamada, D.: "The equilibrium intermediate of beta-lactoglobulin with non-native α-helical Structure" J. Mol. Biol.269. 479-487 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimizu, H.: "Expression of gpsA enocoding biogynthetic su-glycerol-3-phosphate dehydrogenase suppresses both the LB-phenotype of SecB null mutant and cold sensitive phenotype of SecG null mutunt" Mol, Microbiol.26. 1015-1023 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sato, S.: "Expression control of an operon from an intrucellular Symbiont which is homologous to the groE operon" J. Bacteriol.179. 2300-2304 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Taguchi, H.: "ATP-K^+-dependent Heptamer exchange reaction produces Hybrids bctween GroEL and chaperogin from Thernus thermophilus" J. Biol. Chem.272. 18155-18160 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Goto, Y.: "Molecular Chaperones in the life cycle of proteins" Marcel Dekker, New York, 626 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tokuda, H.: "Structure, function and Expression control" Kyusyu University Press Ikarger Medical and Scientific Publishers, 190 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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