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Regulation of cellular Function by molecular chaperones

Research Project

Project/Area Number 09276102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAGATA Kazuhiro  Kyoto Univ., Inst. for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (50127114)

Co-Investigator(Kenkyū-buntansha) ITO Koreaki  Kyoto Univ., Inst. for Virus Research, Professor, ウイルス研究所, 教授 (90027334)
MORI Masataka  Kumamoto Univ., School of Medicine, Professor, 医学部, 教授 (40009650)
YOSHIDA Masasuke  Chemical Res. Lab. Tokyo Inst. of Technology, Professor, 資源化学研究所, 教授 (90049073)
ENDO Toshiya  Nagoya Univ., Graduate School of Science, Professor, 大学院・理学研究科, 教授 (70152014)
MIHARA Katsuyoshi  Kyushu Univ., Graduate Sch. of Med. Sch. of Med. Sci., Professor, 大学院・医学研究科, 教授 (40029963)
矢原 一郎  東京都臨床医学総合研究所, 副所長 (60109957)
Project Period (FY) 1997 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥85,600,000 (Direct Cost: ¥85,600,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥22,000,000 (Direct Cost: ¥22,000,000)
Fiscal Year 1999: ¥21,700,000 (Direct Cost: ¥21,700,000)
Fiscal Year 1998: ¥21,900,000 (Direct Cost: ¥21,900,000)
Fiscal Year 1997: ¥17,000,000 (Direct Cost: ¥17,000,000)
Keywordsmolecular chaperone / stress proteins / protein folding / quality control / heat shock proteins / chaperonin / transport / prion / 細胞内輸送 / 細胞機能制御 / Quality Control
Research Abstract

This group tried to well-organize the studies performed among three groups : "Dynamic interaction between chaperone and its substrates", "Roles of molecular chaperones in cell function" and the selected research groups from applicants.
1. We held the group meeting twice a year and discussed about the progress of each research as well as perspective and direction of the whole research groups.
2. We issued the "Chaperone newsletter" twice a year to stimulate the exchange of the information among the members. In addition to the 9 issues of these newsletters, we also published a special issue on "Chaperone-related ATPases". These newsletters brought broad interest and impact to general readers outside this field.
3. We organized a general meeting for all the members every year and active discussion was performed on the progress of each research by the attendants including the members.
4. We organized a international congress titled "Molecular Chaperone 2000" in Tokyo and two symposiums and two workshops during these 5 years.

Report

(6 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] Taguchi, H., et al.: "Shingle-molecule observation of protein-protein interactions in the chaperonin system"Nat.Biotechnol.. 19. 861-865 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishikawa, S., et al.: "Molecular chaperones in the yeast ER maintain the solubility of proteins for retro-translocation and degradation"J.Cell Biol.. 153. 1061-1039 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hosokawa, N., et al.: "A novel ER α-mannosidase-like protein accelerates ER-associated degradation"EMBO Reports. 2. 415-422 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi, T., et al.: "Respiratory chain strongly oxidizes the CXXC motif of DsbB in the Escherichia coli disulfide-bond formation pathway"EMBO J.. 18. 1192-1198 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ota, K., et al.: "Forced transmembrane orientation of hydrophilic polypeptide segments in multispanning menbrane proteins"Molecular Cell. 2. 495-503 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada, K., et al.: "The human Dna J homologue dj2 facilitates mitochondrial protein import and luciferase refolding"J.Cell Biol.. 139. 1089-1095 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 永田和宏, 森正敬, 吉田賢右: "分子シャペロンによる細胞機能制御"シュプリンガー・フェアラーク東京. 219 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taguchi,H.,et al.: "Shingle-molecule observation of protein-protein interactions in the chaperonin system."Nat. Biotechnol.. 19. 861-865 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishikawa,S.,et al.: "Molecular chaperones in the yeast ER maintain the solubility of proteins for retro-translocation and degradation."J. Cell Biol.. 153. 1061-1039 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hosokawa,N.,et al.: "A novel ER α-mannosidase-like protein accelerates ER-associated degradation."EMBO Reports. 2. 415-422 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi,T.,et al.: "Respiratory chain strongly oxidizes the CXXC motif of DsbB in the Escherichia coli disulfide-bond formation pathway."EMBO J.. 18. 1192-1198 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ota,K.,et al.: "Forced transmembrane orientation of hydrophilic polypeptide segments in multispanning menbrane proteins."Molecular Cell. 2. 495-503 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada,K.,et al.: "The human Dna J homologue dj2 facilitates mitochondrial protein import and luciferase refolding."J. Cell Biol.. 139. 1089-1095 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eds.Nagata,K.,Mori,M.,Yoshida,M.: "Regulation of cellular function by molecular chaperones."Springer-Verlag Tokyo. 219 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.MURAKAMI: "Heat shock protein(HSP)47 and collagen are upregulated during neointimal formation in the balloon-injured rat carotid artery"Atherosclerosis. 157. 361-368 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.KIKUCHI: "A single chain Fv fragment 2A3 specific for native lysozyrme : Isolation from a human synthetic phage display antibody library and characterization"J. Biochem.. 129. 237-242 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] O.MATSUSHITA: "Substrate recognition by the collagen-binding domain of clostridium histolyticum class I collagenase"J. Biol. Chem.. 276(12). 8761-8770 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.NAITOH: "Upregulation of HSP47 and collagen type III in the dermal fibrotic desease, keloid"Biochem Biophys Res Commun.. 280. 1316-1322 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.HOSOKAWA: "A novel ER α-mannosidase-like protein accelerates ER-associated degradation"EMBO Reports. 2(5). 415-422 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.NAKATSUKASA: "Mnl1p, an α-mannosidase-like protein in yeast Saccaromyces cerevisiae, is required for ER associated degradation of glycoproteins"J. Biol. Chem.. 276(12). 8635-8638 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.YOKOTA: "Benzylidene lactam compound, KNK437, a novel inhibitor of acquisition of thermotolerance and heat shock protein induction in human colon carcinoma cells."Cancer Research. 60. 2942-2948 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.KOIDE: "Conformational requirements of collagenous peptides for recognition by the chaperone protein HSP47."J.Biol.Chem.. 275(36). 27957-27963 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.NAGAI: "Embryonic lethality of molecular chaperone hsp47 knockout mice is associated with defects in collagen biosynthesis."J.Cell Biol.. 150(6). 1499-1505 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] O.MATSUSHITA: "Substrate recognition by the collagen-binding domain of clostridium histolyticum class I collagenase."J.Biol.Chem.. 276(12). 8761-8770 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.NAITOH: "Upregulation of HSP47 and collagen type III in the dermal fibrotic desease, keloid"Biochem Biophys Res Commun.. 280. 1316-1322 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.HOSOKAWA: "A novel ER α -mannosidase-like protein accelerates ER-associated degradation."EMBO Reports. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] K.NAGATA: ""Real-Time Analysis of Biomolecular Interactions" (Eds.K Nagat a & H.Handa)"Springer. 3-9 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] J.NISHIZAWA: ""Thermot herapy for Neoplasia, Inflammation, and Pain" (Eds.M Kosaka et al.)"Springer. 382-391 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M. TANABE: "The mammalian HSF4 gene generates both an activator and a repressor of heat shock genes by alternative splicing"J. Biol. Chem.. 274(39). 27845-27856 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H. HIRATA: "Separate cis-acting DNA elements control cell type-and tissue-specific expression of collagen-binding molecular chaperone HSP47"J. Biol. Chem.. 274(50). 35703-35710 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. KOIDE: "Substrate recognition of collagen-specific molecular chaperone HSP47 : Structural requirements and binding regulation"J. Biol. Chem.. 274(49). 34523-34526 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. KAWAZOE: "HSF3 is a major heat shock responsive factor during chicken embryonic development"Eur. J. Biochem.. 265(2). 688-697 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. ASADA: "Effect of HSP47 on prolyl 4-hydroxylation of collagen model peptides"Cell Struct. Funct.. 24(4). 187-196 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N. HOSOKAWA: "Procollagen binding to both prolyl 4-hydroxylase/protein disulfide isomerase and HSP47 within the endoplasmic reticulum in the absence of ascorbate"FEBS. Letters.. 466(1). 19-25 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.TANABE: "Disruption of the HSF3 gene results in the severe reduction of heat shock gene expression and loss of thermotolerance." EMBO J.17 (6). 1750-1758 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] I.YAMAMURA: "Transcriptional activation of the mouse HSP47 gene in mouse osteoblast MC3T3-E1 cells by TGF-β1." Biochem Biophys Res Commun.244. 68-74 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.KAWAZOE: "Proteasome inhibition leads to the activation of all members of the heat-shock-factor family." Eur J Biochem.255. 356-362 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.MASUDA: "Expression and localization of collagen-binding stress protein HSP47 in mouse embryo development : Comparison with types I and II collagen." Cell Stress & Chaperones.3 (4). 256-264 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.NISHIZAWA: "Reactive Oxygen Species Play an Important Role in the Activation of Haet Shock Factor 1 in Ischemia-Reperfused Heart." Circulation.99 (7). 934-941 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.NAGAI: "Human genome has a functional hsp47 gene (CBP2) and pseudogene (pshsp47)." Gene.227. 241-248 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.NISHIZAWA: "Handbook of Experimental Pharmacology “Stress Proteins"" Springer-Verlag, 24 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.NISHIZAWA: "Stress proteins in myocardial protection (in press)" Landes Bioscience, (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.TANABE, K.NAGATA: "Disruption of the HSF3 gene results in the severe reduction of heat shock gene expression and loss of thermotolerance." The EMBO J.in.press.

    • Related Report
      1997 Annual Research Report
  • [Publications] A.NAKAI, K.NAGATA: "HSF4,a new member of the human heat shock factor gene family which lacks properties of a transcriptional activator." Mol.Cell.Biol.17(1). 469-481 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Makino, M.YOSHIDA: "Chaperonin-mediated folding of green fluorescent protein." J.Biol.Chem.272. 12468-12474 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Terada, M.MORI: "The human DanJ homologue dj2 facilitates mitochondrial protein import and luciferase refolding." J.Cell Biol.139. 1089-1095 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.Matsumoto, K.ITO: "SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia. coli plasma membrane." EMBO J.16. 6384-6393 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kanamori, T.ENDO: "Probing the environment along the protein import pathways in yeast mitochondria by site-specific photocrosslinking." Proc.Natl.Acad.Sci.USA. 94. 485-490 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.NAGATA: "Molecular Chaperones in Proteins ; Structure,Function,and Mode of Action." (Eds.Fink A L, Goto Y), (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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