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Molecular Basis for RNA Dynamic Function

Research Project

Project/Area Number 09278101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

WATANABE Kimitsuna  University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (00134502)

Co-Investigator(Kenkyū-buntansha) OHTA Shigeo  Nippon Medical School, Institute of Gerontology, Professor, 老人病研究所, 教授 (00125832)
INOUE Tan  Kyoto University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (40114855)
NAKAMURA Yoshikazu  University of Tokyo, The Institute of Medica Scie, Professor, 医科学研究所, 教授 (40114590)
SHIMURA Yoshiro  Biomolecular Engineering Research Institute, Director Researcher, 生物分子工学研究所, 所長
SHIMAMOTO Nobuo  Natural Institute of Genetics, Professor, 構造研究部門, 教授 (20127658)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥226,100,000 (Direct Cost: ¥226,100,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥30,600,000 (Direct Cost: ¥30,600,000)
Fiscal Year 1999: ¥42,600,000 (Direct Cost: ¥42,600,000)
Fiscal Year 1998: ¥64,500,000 (Direct Cost: ¥64,500,000)
Fiscal Year 1997: ¥85,400,000 (Direct Cost: ¥85,400,000)
KeywordsRNA Catalytic Function / RNA Information Function / RNA High-level Function / Rybozyme / Translation Factors / Aplicing / Mitochondria / tRNA / 触媒機能 / 情報機能 / 高次機能 / RNAの触媒機能 / RNA動的機能 / mRNAの発現調節 / 分子擬態 / 高次生命現象 / RNA結合蛋白質 / RNAの高次構造 / ミトコンドリア病 / 動的構造の解析法 / PNAの触媒機能 / 高次生命現像
Research Abstract

The aim of this research project is to establish the molecular basis of RNA dynamic functions in the phenomena of life by systematizing the RNA dynamic functions through revealing the molecular mechanisms of catalytic functions of RNA and high-level control functions of information expression, and searching and artificially producing ribozymes catalyzing new reaetions. On the basis of the results obtained by these researches, molecular mechanisms of high-level phenomena of life such as development, differentiation and disease will be pursued and many unknown RNA functions will be elucidated. In parallel with these achievements new methods for analyzing RNA dynamic functions will be developed. For this purpose three research groups and one special team were organized under a master group. The first, 「RNA dynamic functions」 group succeeded in determination of a minimal functional region of group I intron possessing the splicing function, construction of dimeric hammerhead ribozyme (maxiz … More yme) providing both sequence recognition and RNA scission activities, and expression of the complex of the maxizyme with a helicase in cells. The second, 「RNA information fuctions」group obtained a highly appreciated result of discovery of tRNA molecular mimicry by translation factors」 which leads to elucidating the basic principle of the translation reaction. In research for RNA recognition mechanisms by proteins, NMR analysis of a complex of sxl protein with a single-stranded RNA and X-ray analysis of a complex of aminoacyl-tRNA synthetase with tRNA succeeded in elucidating molecular recognition mechanisms of RNA by proteins. The third, 「RNA high-level functions」 succeeded in constructing a mitochondrial gene-introduced mouse useful for elucidating the cause of disease and mechanism of senescence. It was found that mitochondrial diseases are caused by lacking in the taurine-modification at the anticodon first position of mitochondrial tRNAs. The elucidation of functional structures of tmRNA was also successful. The research project has been progressing well and it is estimated that about 75% of the original aims have been achieved. During progress in the research project Japan RNA Society, a supporting system for supporting the RNA research in Japan was established on the basis of the project. Less

Report

(6 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Suzuki, T., Watanabe.K et al: "Structural Compensation for the Deficist of rRNA with proteins in the Mammalian into chndinal Ribosome"J.Biol.Chem.. 276. 21724-21736 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito, K., Uno.M, Nakamura, Y: "A tripeptide 'anticodon' deciphens stop codons in message RoSA"Nature. 403. 680-684 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikawa, Y, Inoue, T.et al: "Minimal Catalyfic domain of a group I self aplicing intron RNA"Nature Str.Biol.. 7. 1032-1035 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, K., Ohta, S et al: "Alzheiner's disease and DLST genotype"The Lancet. 350. 1367-1368 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada, Y., Shimamoto N.et al: "Direct observation of DNA refation during tiarscoption by Escherichia Coli RNA polymerace"Nature. 409. 113-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakada, K., Hayashi, J, et al: "Inter-mitochondrial complementation:mitochondria specific system preventing mice from…"Nature Med.. 7. 934-939 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 渡辺, 中村, 井上他: "志村、渡辺共編「RNA研究の最前線」"シュプリングフェアラーク東京. 238 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, T., Watanabe, K. et al.: "Structural Compensation for the deficit of rRNA with Proteins in the mammalian mitochondrial ribosome."J. Biol. Chem.. 276. 21724-21736 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito, K., Uno, M. and Nakamura, Y.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA"Nature. 403. 680-684 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikawa, Y., Inoue, T. et al.: "Minimal catalytic domain of a group I self-splicing intron RNA"Nature Str. Biol.. 7. 1032-1035 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, K., Ohta, S.: "Alzheimer's disease and DLST genotype"The lancet. 350. 1367-1368 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada, Y., Shimamoto, N. et al.: "Direct observation of DNA rotation during transcription by Escherichia coli RNA polymerase"Nature. 409. 113-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakada, K., Hayashi, J. et al.: "Inter-mitochondrial Complementation : mitochondria-specific sysytem preventing mice from expression of disease phenotypes by mutant mtDNA"Nature Med.. 7. 934-939 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Handa, N., Yokoyama, S. et al.: "Structural basis for Recognition of the tra mRNA precursor by the Sex-lethal protein"Nature. 398. 579-585 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogawa, T., Watanabe, K., Masaki, H. et al.: "A cytotoxic ribonuclease targeting specific transfer RNA anticodons"Science. 283. 2097-2100 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikawa, Y., Shiraishi, H., Inoue, T: "Minimal cat alytic domain of a group I. self-splicing imtron RNA"Nature structural biology. 7. 1032-1035 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanabe, T., Taira, K.et al.: "Oncogenic inactivation in a mouse model : Tissue invasion by leukoemic cells is stalled by"Nature. 406. 473-474 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ito, K., Uno, M., Nakamura, Y.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA"Nature. 403. 680-684 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Handa, M., Yokoyama, S et al.: "Structural basis for recognition of the mRNA preursor by the Sex-lethal protein"Nature. 398. 579-585 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasukawa, Y., Watanabe, K.et al.: "Wobble modification defect disturbs codon-anticodon interaction in a mitochondrial disease"EMBO J.. 20. 4794-4802 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Inoue, K., Hayashi, J et al.: "Generation of mice with mitochondrial dysfunction by introducing mouse mtDNA carrying a deletion"Nature Genetics. 26. 176-181 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉村, 渡辺 共編: "RNA 研究の最前線"スプリングフェアラーク東京. 238 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomita,K. et al.: "A cytotoric ribonuclease with specifically cleaves four is accepting arginine tRNAs at their anticaln loops"Proc.Nate.Acad.&ci.,USA. 97・15. 8278-8283 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakamura,Y.,Ito,K.and Ehrenberg,M.: "Mimicry grasps reality in translation termination"Cell. 101. 349-352 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okawa,Y.,Shiraishi,H.and Onoue,T.: "ninimal a cetalytic domain of a group 2 self splicing intron RNA"Nature Structural Biology. 7・11. 1032-1035 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sen,a.Nagai,H.and Shimamoto,N.: "Potmerasc-arrest at the lambda PR promoter during transcription initiation"J.Biol.Chem.. 275. 10899-10904 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugiura.I. et al.: "The 2.0-A crystal Structure of Thermus thermophilus Methionyl-tRNA synthetase reveals two RNA-binding modules"Structure. 8. 197-208 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Inoue,K. et al: "Generation of mice coith mitochondrial dysfunction by introducing mouse mtDNA carrying a deletion intoring gotes"Nature Genetics. 26. 176-181 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 志村令郎,渡辺公網 共編: "RNA研究の最前線"シュプリンガー・フュアラーク東京. 238 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yasukawa, T. et al.: "Modification defect at Anticodon wobble nucleotide of Mitochondrial tRNAs Leu (UUR) with Pathogenic mutations"J. Biol. Chem. 275 b. 4251-4257 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikawa, Y. et al.: "A conserved motif in group IC3 introns is a new class of GNRA receptor"Nucleic Acids Res.. 27. 1859-1865 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ito, K. et al.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA"Nature. 680-684 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimamoto, N.: "One dimensional diffusion of proteins along DNA"J. Biol. Chem.. 274. 15293-15296 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ito, S. et al.: "Functional integrity of mt genomes in human platelets and actopsied brain tissues"Proc. Natl. Acad. Sci. USA. 96. 2099-2103 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tagami, H., Aiba, H., et al.: "An inactive open complex mediated by CP element of E. coli promoter"Proc. Natl. Acad. Sci. USA. 96. 12914-12919 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Editor in Chief T : Creighton (分担): "The Encyclopedia of Molecular Biology"WILEY. 3500 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naito,Y.et al.: "P5abc of the Tetrahymena ribozyme consists of three functionally independent elements" RNA. 4. 837-846 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nitta,I.et al: "A novel cell-free System for Peptide synthesis driven by pyridine" Biol.Chem.379. 819-829 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ito,K.et al.: "Single aminoacid substitution in prokaryote polykeptide RF2 permits it to terminate translation" Proc.Natl.Acad.Sci.,USA. 95. 8165-8169 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tagami,H & Aiba,H: "A comnon role of CRP in transcription activation CRP acts transiantly to stimulate events" EMBO J.17. 1759-1767 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Muto,A.et al.: "A bacterial RNA that functions as both a tRNA and an mRNA" Trends in Biochem.Sei.23. 25-29 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nureki,O.et al.: "Enzyme structune with two catalyfic sites for doublesieve selection of substrate" Science. 280. 578-582 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 嶋本,仰編 中村.嶋本他著: "遺伝子の構造生物学" 共立出版, 208 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] I.Hayashi et al: "Expression of bovine mitoohondrial tRNA^<ser> GCU deriratives in Escherichia coli" Nucleic Acids Res.25. 3505-3507 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Matsuyama et al: "Anovel Cocble rule found in starfish mitochordria" J.Biol.Chem.273、6. 3363-3368 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] K,Mita et al: "Comprehonsive cloning of Schirosacchere myces psmbe genes encoding tranesdation on elongading factors" Gene. 187. 259-266 (1977)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Ikawa et al: "Long-range interaction between the P2.1 and P9.1 pertpranal domains of the ″Tatoahyment ritsrgne″" Nocleic Acids Res.25. 1761-1765 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] D.A.Grillot et al: "Genemic orgarization,Promoten region anelyeis,and Chromosome localizetional of the mouse bcl-x gene" J.Immunol.158. 4750-4751 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kubori et al: "Physical interjerence beerseen Esderichia cols RNA polynerase mdecctles bing in forndeno enhencrs" Nucleic Acile Res.25. 2640-2647 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大澤著、渡辺他次: "遺伝暗号の起源と近代" 共立出版, 252 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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