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Molecular basis of RNA function

Research Project

Project/Area Number 09278102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

NAKAMURA Yoshikazu  Institute of Medical Science, The University of Tokyo, Professor, 医科学研究所, 教授 (40114590)

Co-Investigator(Kenkyū-buntansha) MATSUFUJI Senya  The Jikei University, Faculty of Medicine, Professor, 医学部, 教授 (50192753)
KUCHINO Yoshiyuki  National Cancer Center, Biophysics Division, Chief(Researcher), 部長(研究職) (60124418)
AIBA Hiroji  Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (20025662)
YOKOYAMA Shigeyuki  Graduate School of Science, Faculty of Science, The University of Tokyo, Professor, 大学院・理学系研究科, 教授 (00159229)
INOUE Kunio  Nara Institute of Science and Technology, Research Associate, 助手 (40252415)
豊田 春香  東京大学, 医科学研究所, 講師 (10197973)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥206,400,000 (Direct Cost: ¥206,400,000)
Fiscal Year 2000: ¥52,700,000 (Direct Cost: ¥52,700,000)
Fiscal Year 1999: ¥52,700,000 (Direct Cost: ¥52,700,000)
Fiscal Year 1998: ¥50,000,000 (Direct Cost: ¥50,000,000)
Fiscal Year 1997: ¥51,000,000 (Direct Cost: ¥51,000,000)
KeywordsRNA / translational control / posttranscriptional control / ribosome / molecular mimicry / stop codon / life of mRNA / RNA-binding protein / 終止コドン
Research Abstract

RNA plays a fundamental key role in life as a variety of regulatory and catalytic molecules involved in gene expression and cellular regulation. Here, we have studies molecular bases of dynamic RNA functions by focusing on RNA-protein complexes, regulatory RNAs for gene expression, splicing, transport and translation. The remarkable progress in functional and structural biology of translational apparatus has uncovered a novel biological phenomenon of macromolecular mimicry between protein and tRNA. The 3D struetures of translational elongation factor EF-G and ribosome recycling factor RRF are shown to mimic a tRNA shape. Another, even more sophisticated, example may be polypeptide release factors that are shown to possess a tripeptide 'anticodon' to decipher stop codons in mRNA, showing an impressive mimic of tRNA function. It is surprising that it took 4 decades since the discovery of the genetic code to figure out the basic mechanisms behind the deciphering of its 64 codons. Other achievements in the translational control - such as mammalian frameshift control, trans-translation with tmRNA in bacteria, and cell proliferation induced by the abnormality in the mammalian translation initiation factors - as well as in mRNA export, RNA-binding proteins involved in cell differentiation and RNA-protein complex structures, have uncovered numerous novel mechanisms of gene expression and RNA functions. These accomplishments clearly emphasized the biological importance and interest of the regulatory role of RNA and the mechanisms underlying the post-transcriptional control of gene expression. Certainly, understanding of RNA information flow has increasing importance in the post-genome era. A future emerging understanding of spatiotemporal networking of diverse functional RNAs Will greatly facilitate post-genome research as well as structural biology, while expanding our knowledge of how deeply and universally RNA constitutes life.

Report

(5 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (111 results)

All Other

All Publications (111 results)

  • [Publications] Ito, K.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA"Nature. 403. 680-684 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, Y.: "Mimicry grasps reality in translation termination"Cell. 101. 349-352 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wilson, K.: "Functional sites of interaction between release factor RF1 and the ribosome"Nature Struct.Biol.. 7. 866-870 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toyoda, T.: "Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch"RNA. 6. 1432-1444 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abo, T.: "SsrA-mediated tagging and proteolysis of LacI and its role in the regulation of lac operon"EMBO J.. 19. 3762-3769 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito, K.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA."Nature. 403. 680-684 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, Y.: "Mimicry grasps reality in translation termination."Cell. 101. 349-352 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wilson, K.: "Functional sites of interaction between release factor RF1 and the ribosome."Nature Struct. Biol.. 7. 866-870 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toyoda, T.: "Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch."RNA. 6. 1432-1444 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abo, T.: "SsrA-mediated tagging and proteolysis of LacI and its role in the regulation of lac operon."EMBO J.. 19. 3762-3769 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito,K.: "A tripeptide 'anticodon' deciphers stop codons in messenger RNA."Nature. 403. 680-684 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakamura,Y.: "Mimicry grasps reality in translation termination."Cell. 101. 349-352 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Wilson,K.: "Functional sites of interaction between release factor RF1 and the ribosome."Nature Struct.Biol.. 7. 866-870 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanaka,R.: "Intronic U50 small-nucleolar-RNA (snoRNA) host gene of no protein-coding potential is mapped at the chromosome breakpoint t(3;6)(q27;q15) of human B-cell lymphoma."Genes Cells. 5. 277-287 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tin,O.F.: "Proteolytic fragmentation of polypeptide release factor 1 of Thermus hermophilus and crystallization of the stable fragments."Biochimie. 82. 765-772 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toyoda,T.: "Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch."RNA. 6. 1432-1444 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishino,T.: "Interaction of ribosome recycling factor and elongation factor EF-G with E.coli ribosomes studied by surface plasmon resonance technique."Genes Cells. 5. 953-963 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujiwara,T.: "Functional mapping of the ribosome contact site in the ribosome recycling factor."RNA. 7. 64-70 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Abo,T.: "SsrA-mediated tagging and proteolysis of LacI and its role in the regulation of lac operon."EMBO J.. 19. 3762-3769 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tanaka,Y: "A novel regulatory role of glucose tansporter of Escherichia coli : membrane sequestration of a global repressor Mlc."EMBO J.. 19. 5344-5352 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki,H.: "Vegetal localization of the maternal mRNA encoding an EDEN-BP/Bruno-like protein in zebrafish."Mech.Dev.. 93. 205-209 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sekine,S.: "Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase."Nature Struct.Biology. (In Press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakamoto, S.: "Molecular Computation by DNA Hairpin Formation."Science. 288. 1223-1226 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukai,S.: "Structural basis for double-sieve discrimination of L-valine from L-isoleucine and L-threonine by the complex of tRNA (Val) and valyl-tRNA synthetase"Cell. 103. 793-803 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Itoh,T.: "Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis."Science. 291. 1047-1051 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakamura,Y.: "Genetic probes to bacterial release factors : tRNA mimicry hypothesis and beyond. In : The Ribosome : Structure, Function, Antibiotics and Ceuular Interactions"The American Society for Microbiology, Washington, D.C.. 519-526 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Crawford, D.-J. G., Ito, K., Nakamura, Y., Tate, W. P.: "Indirect regulation of termination efficiency at highly expressed genes and recoding sites by the factor recycling function of Escherichia coli release factor RF3"EMBO J.. 18. 727-732 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ebihara, K., Nakamura, Y.: "C-terminal interaction of translational release factors eRF1 and eRF3 of fission yeast : G-domain uncoupled binding and the role of conserved amino acids"RNA. 5. 739-750 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujiwara, T., Ito, K., Nakayashiki, T., Nakamura, Y.: "Amber mutations in ribosome recycling factors of Escherichia coli and Thermus thermophilus : evidence for C-terminal modulator element"FEBS Lett.. 447. 297-302 (1999297)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimura, K., Ito, K., Nakamura, Y.: "Amber (UAG) suppressors affected in UGA/UAA-specific polypeptide release factor 2 of bacteria : genetic prediction of initial binding to ribosome preceding stop codon recognition"Genes Cells. 4. 253-266 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Powell, B., Peters III, H. K., Nakamura, Y., Court, D.: "Cloning and analysis of the rnc-era-recO operon from Pseudomonas aeruginosa"J. Bacteriol.. 181. 5111-5113 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Karamyshev, A., Ito, K., Nakamura, Y.: "Polypeptide release factor eRF1 from Tetrahymena thermophila : cDNA cloning, purification and complex formation with yeast eRF3"FEBS Lett.. 457. 483-488 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Karamyshev, A., Karamycheva, Z., Ito, K., Matsufuji, S., Nakamura, Y.: "Overexpression and purification of recombinant eRF1 proteins of rabbit and Tetrahymena thermophila"Biochemistry (Moscow). 64. 1648-1658 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wada, M., Nakamura, Y.: "Type-II major-surface-glycoprotein family of Pneumocystis carinii"J. Euk. Microbiol.. 46. 151S-152S (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wada, M., Nakamura, Y.: "Immunological characterization of surface subtilisin-like protease (SSP) of Pneumocystis carinii"J. Euk. Microbiol.. 46. 125S-126S (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wada, M., Nakamura, Y.: "Type-II major-surface-glycoprotein family of Pneumocystis carinii under the control of novel expression elements"DNA Res.. 6. 211-217 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tin, O. F., Rykunova, A. L., Muranova, T. A., Toyoda, T., Ito, K., Suzuki, T., Watanabe, K., Garber, M. B., Nakamura, Y.: "Proteolytic fragmentation of polypeptide release factor 1 of Thermus thermophilus and crystallization of the stable fragments"Biochimie, In Press. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka, R., Satoh, H., Moriyama, M., Satoh, K., Morishita, Y., Yoshida, S., Watanabe, T., Nakamura, Y., Mori, S.: "Intronic U50 small-nucleolar-RNA (snoRNA) host gene of no protein-coding potential is mapped at the chromosome breakpoint t(3;6)(q27;q15) of human B-cell lymphoma"Genes Cells, In Press. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ito, K., Uno, M., Nakamura, Y.: "A tripeptide 'anticodon deciphers' stop codons in messenger RNA"Nature. 403. 680-683 (200)

    • Related Report
      1999 Annual Research Report
  • [Publications] Abe, H., Abo, T., Aiba, H.: "Regulation of intrinsic terminator by translation in Escherichia coli : transcription termination at a distance downstream"Genes to Cells. 4. 87-97 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tagami, H., Aiba, H.: "An inactive open complex mediated by UP element ant Escherichia coli promoter"Proc. Natl. Acad. Sci. USA. 96. 12914-12919 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka, Y., Kimata, K., Inada, T., Tagami, H., Aiba, H.: "Negative regulation of the pts operon by Mls : Mechanism underlying glucose induction in Escherichia coli"Genes to Cells. 4. 391-399 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugiyama, A., Noguchi, K., Kitanaka, C., Katou, N., Tashiro, F., Ono, T., Yoshida, M. C., Kuchino, Y.: "Molecular cloning and chromosomal mapping of mouse intronless myc gene acting as a potent apoptosis inducer"Gene. 226. 273-283 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mochizuki, T., Kitanaka, C., Noguchi, K., Muramatsu, T., Asai, A., Kuchino, Y.: "Physical and functional interaction between Pim-1 kinase and Cdc25A phosphatase : implication for the Pim-1-mediated activation of the c-Myc signaling pathway"J. Biol. Chem.. 274. 18659-18666 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noguchi, K., Kitanaka, C., Yamana, H., Kokubu, A., Mochizuki, T., Kuchino, Y.: "Regulation of c-Myc through phosphorylation at Ser-62 and -71 by c-Jun N-terminal kinase"J. Biol. Chem.. 274. 32580-32587 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe, S., Muramatsu, T., Ao, H., Hirayama, Y., Takahashi, K., Tanokura, M., Kuchino, Y.: "Molecular cloning of the Lon protease gene form Thermus thermophilus HB8 and characterization of its gene product"E. J. Biochem.. 266. 811-819 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Itoh, S., Hattori, T., Hayashi, H., Mizutani, Y., Todo, M., Takii, T., Yang, D., Lee, J. C., Matsufuji, S., Murakami, Y., Chiba, T., Onozaki, K.: "Antiproliferative effect of IL-1 is mediated by p38 mitogen-activated protein kinase in human melanoma cell A 375"J. Immunol.. 162(12). 7434-7440 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murakami, Y., Matsufuji, S., Hayashi, S., Tanahashi, N., Tanaka, K.: "ATP-dependent inactivation and sequestration of ornithine decarboxylase by the 26S proteasome is a prerequisite for degradation"Mol. Cell. Biol.. 19. 7216-7227 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suka, M., Matsufuji, S., Murakami, Y.: "Dietary induction of ornithine decarboxylase in male mouse kidney"Biochim. Biophys. Acta.. 1472. 455-461 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saito, T., Hascilowicz, T., Ohkido, I., Kikuchi, Y., Okamoto, H., Hayashi, S., Murakami, Y., Matsufuji, S.: "Two zebrafish antizymes with different expression and activities"Biochem. J.. 345. 99-106 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Isoyama, T., Kamoshita, N., Yasui, K., Iwai, A., Shiroki, K., Toyoda, H., Yamada, A., Takasaki, Y., Nomoto, A.: "Lower concentration of La protein required for internal ribosomal entry onhepatitis C virus RNA than on poliovirus RNA"J. Gen. Virol.. 80(9). 2319-2328 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Maegawa, S., Yasuda, K., Inoue, K.: "Maternal mRNA localization of zebrafish DAZ-like gene"Mech. Dev.. 81. 223-226 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Handa, N., Nureki, O., et al.: "Structural bases for recognition of the tra mRNA precursor by the Sex-lethal protein"Nature. 398. 579-585 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kigawa, T., et al.: "Cell-free production and stable Oisotope labeling of milligram-quantities of proteins"FEBS Lett.. 442. 15-19 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chi, S.-W., et al.: "Chemical shift perturbation studies of the Interactions of the second RNA-binding domain of the Drosophila Sex-lethal protein with the transformer pre-mRNA polyuridine tract and 3' splice site sequences"Eur. J. Biochem.. 260. 649-660 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chi, S., et al.: "Oncogenic Ras triggers cell suicide through the activation of a caspase-independent cell death program in human cancer cells"Oncogene. 18. 2281-2290 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Horie, N., et al.: "Modified nucleosides in the first positions of the anticodons of tRNALeu4 and tRNALeu5 from Escherichia coli"Biochemistry. 38. 207-217 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Atkins, J. F., Bock, A., Matsufuji, S., Gesteland, R. F.: "Dynamics of the Genetic Code"Cold Spring Habor Laboratory Press. 709 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakamura,Y.,Wada,M.: "Molecular pathobiology and antigenic variation of Pneumocystis carinit." Adv.Parasitol.41. 63-107 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Pel.H.J.,Moffat,J.G.Ito,k.,Nakamura,Y.,Tate,W.P.: "Escherichia coli release factor-3:resolving the paradox of a typical G protein structure and atypical function with guanine nucleotides." RNA. 4. 47-54 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakamura,Y.: "The inajor surface antigen of Pneumocystis carinii." FEMS Immun.Med.Microbiol.22. 67-74 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakamura,Y.: "Molecular microbiology of Pneumocystis carinii." Jps.J.Med.Mycol.39. 91-98 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Dasgupta,S.,Fernandez,L.,Kameyama,L.,Inada,T.,Nakamura,Y.,Pappas,A.,Court,D.: "Genetic uncoupling of the dsRNA-binding and RNA-cleavage activities of the Escherichia coli endonbonuclease RNaseIII-the effect of dsRNA binding on gene expression." Mol.Microbiol.28. 629-640 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ito,K.,Ebihara,K.,Nakamura,Y.: "The stretch of C-terminal acidic amino acids of translational release factor eRF1 is a primary binding site for eRF3 of fission yeast." RNA. 4. 958-972 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ito,K.,Uno,M.,Nakamura,Y.: "Single amino acid substitution in prokaryote polypeptide release factor 2 permits it to terminate translation at all three stop codons." Proc.Natl.Acad.Sci.USA. 95. 8165-8169 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakamura,Y.,Ito,K.: "How protein reads the stop codon and terminates translation(Review)." Genes Cells. 3. 263-278 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mita,K.,Morimyo,M.,Hongo,E.,Higashi,T.,Sugaya,K.,Ajimura,M.,Ishihara,Y.,Inoue,H.,Sasanuma,S.,Nohata,J.,Kimura,T.,Koike,Y.,Ito,K.,Nakamura,Y.: "The cDNA and intron catalog of ribosomal protein genes of Schizosaccharomyces pombe." Nucl.Acids Res,In Press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Crawford,D.J.G.,Ito,K.,Nakamura,Y.,Tate,W.P.: "Indirect regulation of termination efficiency at highly expressed genes and recoding sites by the factor recycling function of Escherichia coli release factor RF3." EMBO J.18. 727-732 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Hanaki,N.Odawara,Y.K.Nakajima,C.Shimizu,K.Nozaki,T.Mizuno,T.Muramatsu,Y.kuchino and H.Yoshikura: "Two different reactions involved in the primer/template-independent polymerization of dATP and dTTP by Taq DNA polymerase." Biochemical Biophysical Research Communications. 244. 210-219 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Mishima,S.Higashiyama,A.Asai,K.Yamaoka,Y.Nagashima,N.Taniguchi,C.Kitanaka,T.Kirino and Y.Kuchino: "Heparin-binding epidermal growth factor-like growth factor stimulates mitogenic signaling and is highly expressed in human malignant gliomas." Acta Neuropathologica. 96. 322-328 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.sugiyama,K.Noguchi,C.Kitanaka,N.Katou,F.Tashiro,M.C.Yoshida and Y.Kuchino: "Molecular cloning and chromosomal mapping of mouse intronless myc gene acting as a potent apoptosis inducer." Gene. 226. 273-283 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Chi,C.Kitanaka,K.Noguchi,T.Mochizuki,Y.Nagashima,M.Shirouzu,H.Fujita,M.Yoshida,W.Chen,A.Asai,M.Himeno,S.Yokoyama and Y.Kuchino: "Oncogenic Ras triggers cell suicide through the activation of a caspase-independent cell death program in human cancer cell." Oncogene,in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] C.Kitanaka,Y.Kuchino: "New insight into the signaling pathway for programmed cell death'caspase-independent cell death with necrotic morphology" Cell Death & Differentiation,in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Satriano J,Matsufuji S,Murakami Y,Lortie MJ,Schwartz D,Kelly C,Hayashi S and Blantz RC.: "Agmatine suppresses proliferation by frameshift induction of antizyme and attenuation of cellular polyamine levels." J Biol Chem. 273(25). 15313-15316 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ivanov IP,Gesteland RF,Matsufuji S and Atkins JF.: "Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals,predominantly +1 in fission yeast,but -2 in budding yeast." RNA. 4(10). 1230-1238 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shingo Maegawa,Kunio Yasuda,and Kunio Inoue.: "Matemal mRNA localization of zebrafish DAZ-like gene." Mechanisms of Development. 81. 227-230 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tagami,H.and Aiba,H.: "A common role of CRP in transcription activation:CRP acts transiently to stimulate events leading to open complex formation at a diverse set of promoters." EMBO J.17. 1759-1767 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi,H.,Inada,T.,Postma,P.,and Aiba,H.: "CRP down-regulatesadenylate cyclase activity by reducing the level of phosphorylated IIAGlc,the glucose-specific phosphotransferase protein,in Escherichia coli." Mol.Gen.Genet.259. 317-326 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kimata,K.,Inada,T.,Tagami,H.,and Aiba,H.: "A global repressor(Mlc)is involved in glucose induction of the ptsG gene encoding major glucose transporter in Escherichis coli." Mol.Microbiol.29. 1509-1520 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hogema,B.,Arents,J.,Bader,R.,Eijkemans,K.,Inada,T.,Aiba,H.Postma,P.: "Inducerexclusion by glucose 6-phosphate in Escherichia coli." Mol.Microbiol.30. 487-498 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Abe,H.,Abo,T.,and Aiba,H.: "Regulation of intrinsic terminator by translation in Escherichia coli:transcription termination at a distance downstream." Genes to Cells in press.4. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tagami,H.and Aiba,H.: "A novel inactive open complex mediated by UP element at Escherichia coli promoter." Proc.Natl.Acad.Sci.USA in press.96. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwahara,J.,T.Kigawa,K.Kitagawa,H.Masumoto,T.Okazaki and S.Yokoyama: "A helix-turn-helix structure unit in human centromere protein B(CENP-B)." EMBO J.17. 827-837 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Seio,K.,T.Wada,K.Sakamoto,S.Yokoyama,and M.Sekine: "“Chemical synthesis and properties of conformationally fixed diuridne monophosphates as building blocks of the RNA turn motif"" J.Org.Chem.63. 1429-1443 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shirouzu,M.,K.Morinaka,S.Koyama,C.-D.Hu,N.Hori-Tamura,T.Okada,K.Kariya,T.Kataoka,A.Kikuchi,and S.Yokoyama: "“Interactions of the amino acid residue at position 31 of the c-Ha-Ras protein with Raf-1 and RalGDS"" J.Biol.Chem.273. 7737-7742 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nureki,O.,D.G,Vassylyev,M.Tateno,A.Shimada,T.Nakama,S.Fukai,M.Konno,T.L.Hendrickson,P.shimmel,and S.Yokoyama: "Enzyme Structure withe two catalytic sites for double-sieve selection of substrate" Science. 280. 578-582 (1998)

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  • [Publications] Atkins,J.F.,Bock A.,Matsufuji,S.and Gesteland,R.F.: "Dynamics of the Genetic Code" Cold Spring Harbor Laboratory Press., 709 (1998)

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  • [Publications] Mita,K., Morimyo,M., Ito,K., Sugano,K., Ebihara,K., Hongo,E., Higashi,T., Hirayama,Y., Nakamura,Y.: "Comprehensive cloning of Schizosaccharamyces pombe genes encoding translation elougation factors." Gene. 187. 259-266 (1997)

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  • [Publications] Ito,K., Nakamura,Y.: "Cloning and overexpression of polypeptide rerease factor 1 of Thermus thermophilus." Biochimie. 79. 287-292 (1997)

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  • [Publications] Nakamura,Y., Wada,M.: "Molecular pathobiology and antigenic variation of Pneumocystis carinii." Adv.Parasitol. 41. 63-107 (1998)

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  • [Publications] Wada,M., Nakamura,Y.: "Closing and overexpression of cell surface subtilisin-like proteases(SSP)of Pneumocystis carinii." J.Euk.microbiol. 54. 54S- (1997)

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  • [Publications] Wada,M., Nakamura,Y.: "Hetero logous expression of Pneumocystis carinii genes in fission yeast." J.Euk.Microbiol.44. 55S-56S (1997)

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  • [Publications] Moffat,J.G.Pel,II.J. Ito,K., Nakamura,Y., Tate,W.P.: "Escherichia coll release factor-3:resolving the paradox of a typical G protein atructure and atypical guanine nucleotide effects on function" RNA. 4. 47-54 (1998)

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  • [Publications] Nakamura,Y.: "The major surface antigen of Pneumocystis carinii." FEMS Immun.Med.Microbiol.(in press). (1998)

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  • [Publications] Nakamura,Y.: "Molecular microbiology of Pneumocystis carinii." Jpn J.Med.Mycol.(in press). (1998)

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  • [Publications] Desgupta,S., Pernandez,L., Kameyama,L., Inada,T., Nakamura,Y., Pappas,A., Court,D.: "Genetic uncoupling of the dsRNA-binding and RNA-cleavage activities of the Escherichia coli endoribonuclease RNaseIII-the effect of dsRNA binding on gene expression." Mol.Microbiol.(in press). (1998)

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  • [Publications] Ito,K., Ebihara,K., Nakamura,Y.: "Dissection of tRNA mimicry element of protein release factor eRF1 of fission yeast:eRF3 binding is not necessary for eRF1 function and cell viability." RNA. (in press). (1998)

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  • [Publications] Kimata,K.: "CRP-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli." Proc.Natl.Acad.Sci.USA. 94. 12914-12919 (1997)

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  • [Publications] Tagami,H.: "A common role of CRP in transcription activation:CRP acts transiently to stimulate events leading to open complex formation at a diverse set of promoters" EMBOJ.(in press). (1998)

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  • [Publications] Hogema,B.: "Catabolite repression by glucose 6-phosphate,gluconate and lactose in Escherichia coli." Mol.Microbiol.24:857-867(1997). 24. 857-867 (1998)

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  • [Publications] M.Nagane: "Application of antisense ribonucleic acid complementary to O6-methylguanine-deoxyribonucleic acid methyltansferase messenger nbonucleic acid for therapy of malignant gliomas" Neurosurgery. 41. 434-441 (1997)

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  • [Publications] Matsufuji,S.: "Assignment of the human antizyme gene(OAZ)to chromosome 19p13.3 by fluorescence in situ hybridization" Genomics. 38. 102-104 (1996)

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  • [Publications] Koguchi,K.: "Cloning and sequencing of a human cDNA encoding ornithine decarboxylase anti yme inhibites" Biochim.Biophys.Acta. 1353. 209-216 (1997)

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  • [Publications] Hayashi.T.: "Characterization of the human antizyme gene." Gene. 203. 131-139 (1997)

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  • [Publications] Tsuchihara,K.: "Specific Interaction of polypyrimidine tract-bingding protein with the extreme 3'-terminal structure of the Hepatitis C virus genome,the 3′X." J.Virol.71. 6720-6726 (1997)

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  • [Publications] Artinger,M.: "Interaction of goosecoid and brachyury in Xenopus mesoderm patterning." Mech Dev. 65. 187-196 (1997)

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  • [Publications] Kim,Y.: "NMR Analysis of the Hydrogen Bonding Interactions of the RNA-binding Domains of the Drosophila Sex-lethal Protein with Target RNA Fragments with Site-specific[3.15N]Uridine Substintion." Nucleic Acids Res.25. 1565-1569 (1997)

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  • [Publications] S.Koshiba: "The Solution Structure of the Pleckstrin Homology Domain of Mouse Son-of-Sevenless 1 (mSos1)" J.Mol.Biol.269. 579-591 (1997)

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  • [Publications] T.Nishinaka: "An Extended DNA Structure through Deoxyribose-base Stacking Induced by RecA Protein" Proc.Matl.Acad.Sci.USA. 94. 6623-6628 (1997)

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  • [Publications] Y.Ito: "The ″Regional Polysterism″in the GTP-bound From of the Human c-Ha-Ras Protein." Biochemistry. 36. 9109-9119 (1997)

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  • [Publications] M.Inoue: "A Characteristic Arrangement of Aromatie Amino Acid Residues in the Solution Structure of the Amino-terminal RNA-binding Domain of Drosophila Sex-lethal." J.Mol.Biol.272. 82-94 (1997)

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Published: 1997-04-01   Modified: 2016-04-21  

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