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The molecular basis of the higher function of RNA molecules

Research Project

Project/Area Number 09278104
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionInstitute of Gerontology, Nippon Medical School

Principal Investigator

OHTA Shigeo  Department of Biochemistry and Cell Biology, Instutue of Gerontology, Nippon Medical School, Professor, 老人病研究所, 教授 (00125832)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Satoru  Institute of Biological Sciences, Gene Experiment Center, University of Tsukuba, Associate Professor, 生物化学系, 講師 (90225508)
MUTO Akira  Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Professor, 農学生命科学部, 教授 (80034635)
WATANABE Kimitsuna  Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Professor, 大学院・新領域創成科学研究科, 教授 (00134502)
WATANABE Yoshimori  Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Associate Professor, 大学院・理学系研究科, 助教授 (20212326)
OKADA Norihiro  Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Professor, 大学院・生命理工研究科, 教授 (60132982)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥192,800,000 (Direct Cost: ¥192,800,000)
Fiscal Year 2000: ¥48,500,000 (Direct Cost: ¥48,500,000)
Fiscal Year 1999: ¥55,300,000 (Direct Cost: ¥55,300,000)
Fiscal Year 1998: ¥46,000,000 (Direct Cost: ¥46,000,000)
Fiscal Year 1997: ¥43,000,000 (Direct Cost: ¥43,000,000)
Keywordsmitochondrial disease / post-translational modification / germ-line cells / meiosis / tmRNA / tRNA / 高次機能 / RNA / tmRNA / 古細菌 / tRNA / 遺伝子変異 / 発生 / 分化 / ミトコンドリア / mtRNA / ミトコンドリア脳筋症 / trans-translction
Research Abstract

In our group, we have focused interest on the following subjects ; (1)The molecular mechanism on the role of RNA molecules involved in diseases. (2)The molecular mechanism on the roles of RNA molecules involved in development and differentiation. (3)Functional analysis of RNA molecules with a unknown function.
Ohta and Watanabe K. purified several mutant mitochondrial tRNA molecules and determined their structure, where the mutant tRNAs are responsible for mitochondrial diseases. We found that some mutant tRNAs lack modification of nucleotides at the third letter of anticodon and that the modification defect reduced the interaction of tRNA and mRNA, leading the unusual protein synthesis as the cause of the diseases.
Kobayashi found that mitochondrial rRNAs are required for development of cells responsible for the germ-line, and that the rRNA molecules remove out of mitochondria and turn into components of polysome in the cytosole.
Watanabe Y. revealed function of an essential RNA molecule, Mei RNA, for meiosis. Mei RNA binds to a component, which is essential for meiosis, to retain it in the nucleus.
Muto identified the structure of tmRNA which plays dual function of tRNA and mRNA.

Report

(5 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Yasukawa, T., Suzuki, T., Ishii, N., Ohta, S., Watanabe, K: "Wobble modification defect in tRNA disturbs codon-anticodon interaction in a mitochondrial disease"EMBO J.. 4794-4802 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasukawa, T., Suzuki, T, Suzuki, Ta., Ueda, T, Ohta, S., Watanabe, K: "Modification defect at anticodon wobble Nucleotide of Mitochondrial tRNAs ^<LEU>(UUR)with Pathogenic mutations of mitochondrial myopathy,encephalopathy,lactic acidosis,and stroke-like Episodes"J.Biol.Chem.. 275. 4251-4257 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Amikura R., Kashikawa M., Nakamura A., Kobayashi S.: "Presence of mitochondria-type ribosomes outside mitochondria in germ plasm of Drosophila embryos"Proc Nat Acad Sci USA. 98(16). 9133-9138 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nameki N., Felden B., Atkins JF., Gesteland RF., Himeno H., Muto A: "Functional and structural analysis of a pseudoknot upstream of the tag-encoded sequence in E. coli tmRNA"J.Mol.Biol.. 286(3). 733-744 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe, Y., Yokobayashi, S., Yamamoto, M., Nurse, P: "Pre-meiotic S-phase is linked to reductional chromosome segregation and recombination"Nature. 409. 359-363 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe M., Nameki N., Matsuo-Takasaki M., Nishimura S., Okada N.: "tRNA recognition of tRNA-guanine transglycosylase from a hyperthermophilic archaeon,Pyrococcus horikoshii"J.Biol.Chem.. 276(4). 2387-2394 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 渡辺公綱, 太田成男, 武藤アキラ, 小林悟, 渡辺嘉典, 岡田典弘ら: ""RNA研究の最前線"(志村令郎、渡辺公綱)"シュプリンガー・フェアラーク東京. 238 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasukawa, T., Suzuki, T., Ishii, N., Ohta, S., Watanabe, K.: "Wobble modification defect in tRNA disturbs codon-anticodon interaction in a mitochondrial disease."EMBO J.. 20(17). 4794-4802 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasukawa, T., Suzuki, T, Suzuki, Ta., Ueda, T, Ohta, S. Watanabe, K: "Modification defect at anticodon wobble Nucleotide of Mitochondrial tRNAs ^<LEU>(UUR) with Pathogenic mutations of mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like Episodes."J. Biol. Chem.. 275. 4251-4257 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Amikura R. Kashikawa M. Nakamura A. Kobayashi S.: "Presence of mitochondria-type ribosomes outside mitochondria in germ plasm of Drosophila embryos."Proc Nat Acad Sci USA. 98(16). 9133-9138 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nameki N. Felden B. Atkins JF. Gesteland RF. Himeno H. Muto A: "Functional and structural analysis of a pseudoknot upstream of the tag-encoded sequence in E. coli tmRNA."J. Mol. Biol.. 286(3). 733-744 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe,Y.,Yokobayashi,S.,Yamamoto,M.,and Nurse,P: "Pre-meiotic S-phase is linked to reductional chromosome segregation and recombination."Nature. 409. 359-363 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe M. Nameki N. Matsuo-Takasaki M. Nishimura S. Okada N.: "tRNA recognition of tRNA-guanine transglycosylase from a hyperthermophilic archaeon, Pyrococcus horikoshii."J. Biol. Chem.. 276(4). 2387-2394 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasukawa,T.,Suzuki,T.,Suzuki,Ta.,Ueda,T.,Ohta,S.and Watanabe,K.: "Modification Defect at Anticodon Wobble Nucleotide of Mitochondrial tRNAs LEU (UUT) with Pathogenic mutations of Mltochondrial Myopathy, Encephalopathy, Lactic Acidosis, and Stroke-like Episodes."J.Biol.Chem.. 275巻6号. 4251-4257 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Muto,A.Fujihara,K.Ito,J.Matsuno,C.Ushida,H.Himeno: "Requirement of transfer-messenger RNA for the growth of Bacillus subtilis under stresses."Genes to Cells. 5巻. 627-636 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masakatsu Watanabe,Nobukazu Nameki,Mami Matsuo-Takasaki,Susumu Nishimura,and Norihiro Okada: "tRNA Recognition of tRNA-guanine Transglycosylase from a Hyperthermophilic Archaeon, Pyrococcus horikoshii"J.Biol.Chem. 276巻4号. 2387-2394 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Kashikawa,R.Amikura and S.Kobayashi: "Mitochondrial small ribosomal RNA is a component of germinal granules in Xenopus embryos."Mech.Dev.. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Watanabe,Y.,Yokobayashi,S.,Yamamoto,M.,and Nurse,P.: "Pre-meiotic S-phase is linked to reductional chromosome segregation and recombination."Nature. 409巻. 359-363 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shinozaki-Yabana,S.,Watanabe,Y.,and Yamamoto,M.: "Novel WD-repeat protein Miplp facilitates function of the meiotic regulator Mei2p in fission yeast."Mol.Cell.Biol.. 20巻. 1234-1242 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 渡辺公綱,太田成男,武藤あきら,小林悟,渡辺嘉典,岡田典弘 ら: ""RNA研究の最前線"(志村令郎,渡辺公綱編)"シュプリンガー・フェアラーク東京. 238 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 瀬名秀明,太田成男: "ミトコンドリアと生きる"角川書店. 221 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nanbu-Wakao r. , Ohta S. et al.: "Bacterial Cell death induced by human pro-apoptotic Bax is blocked by an RNaseE mutant that functions in an anti-oxidant pathway"Genes to cells.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasukawa T. , Ohta S. et al.: "Modification Defect at Anticodon Wobble Nucleotide of Mitochondrial tTNAs ^<LBU>(UUT)with Pathogenic mutations of mitochondrial myopaty, encephalopathy, lactic acidosis, and stroke-like Episodes"J. Biol. Chem.. 275. 4251-4257 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasukawa T. , Ohta S. et al.: "Defect Modification at the Anticodon Wobble Nucleotide of Mitochondrial tRNALys with the MERRF Encephalomyopathy Pathogenic Mutation"FEBS Letters.. 467. 175-178 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ashoh S. , Ohta S. et al.: "Practicable recovery of plasmids carring cDNA encoding anti-apoptotic factors that had been introduced by particle bombardment into 5-week old rat hippocampal slices"Neuroscience Letters.. 272. 62-66 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ito S. , Ohta S. et al.: "Functional integrity of mitochondrial genomes in human platelets and autopsied brain tissues from elderly patients with Alzheimer's disease"Proc. Natl. Acad. Sci. USA.. 96. 2099-2103 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ogawa T. , Watanabe K. et al.: "A cytotoxic ribonuclease targetting specific transfer RNA anticodons"Science. 283. 2097-2100 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 太田成男: "ミトコンドリアの分子生物学. 「臨床遺伝子医学ガイダンス-分子医学へのアプローチ」(小澤敬也 編)"南山堂. 5 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 太田成男: "Alzheimer病とミトコンドリア. 「Annual Review神経」"中外医学社. 8 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ito S., Ohta S. et al.: "Functional integrity of mitochondrial genomes in human platelets and autopsied brain tissues from elderly patients with Alzheimer's disease." Proc.Natl.Acad.Sci.USA.in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Asoh S., Ohta S. et al.: "A trace amount of the human proapoptotic factor Bax induces bacterial death accompanied by damage of DNA." J Biol Chem.273. 11384-11391. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nitta I., Watanabe K. et al.: "Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNAdomains." Science. 281. 666-669. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Felden B., Muto A. et al.: "Presence and location of modified nucleotides in Escherichia coli tmRNA : structural mimicry with tRNA acceptor brances." EMBO J.17. 3188-3196. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iida T. and Kobayashi S.: "Essential role of mitochondrially-encoded large rRNA for germ line formation in Drosophila embryos." Proc.Natl.Acad.Sci.USA.95. 11274-11278 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamashita A., Watanabe Y. et al.: "RNA-assisted nuclear transport of the meiotic regulator Mei2p in fission yeast." Cell.95. 115-123. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Asoh S and Ohta S: "Bcl-2 Completely Blocks Fas-Mediated Apoptosis in mtDNA-Depleted HeLa Cells." Biochem.Biophys.Res Commun. 237. 659-662 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aritomi M.Ohta S et al.: "Crystal Structure of Rat Bcl-x_L;Implication for the Function of the Bcl-2 Protein Family." J Biol Chem. 272. 27866-27892 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hayashi I,Watanabe K et al: "Expression of bovine mitochondrial tRNASerGCU derivatives in Escherichia" Nucleic Acids Res. 25. 3503-3507 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimimamura M,Okada N et al: "Molecular evidence that whales form a clade within even-toed unguates." Nature(a letter). 338. 666-670 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Himeno H Muto A et al: "In vitro trans-translation mediated by alanine-charged 10Sa RNA." J.Mol Biol. 268. 803-808 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Felden B Muto A et al: "Probing the structure of the E.coli 10Sa RNA(tmRNA)." RNA. 3. 89-103 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 麻生定光、太田成男: "神経精神薬理" アポトーシスにおけるミトコンドリアの新しい役割, 4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 太田成男: "日本医科大学雑誌" アポトーシスと関連疾患, 4 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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