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Immobilization of DNA for Single Molecule Detection

Research Project

Project/Area Number 09304070
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 分離・精製・検出法
Research InstitutionHokkaido University

Principal Investigator

SHIMOMURA Masatsugu  Research Institute of Electric and Science, Hokkaido University, Pro., 電子科学研究所, 教授 (10136525)

Co-Investigator(Kenkyū-buntansha) NISHIKAWA Takehiro  Research Institute of Electric and Science, Hokkaido University, Res.Asso., 電子科学研究所, 助手 (40281836)
KARTHAUS Olaf  Research Institute of Electric and Science, Hokkaido University, Res.Asso., 電子科学研究所, 助手 (80261353)
IJIRO Kuniharu  Research Institute of Electric and Science, Hokkaido University, Asso.Pro., 電子科学研究所, 助教授 (90221762)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥41,900,000 (Direct Cost: ¥41,900,000)
Fiscal Year 1998: ¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 1997: ¥32,500,000 (Direct Cost: ¥32,500,000)
KeywordsDNA / single molecule / fluorescence life time / intercalation / base sequencing / SNOM / polyion complex / single molecule detection
Research Abstract

Double-helical DNA is a supramolecular architecture composed of complementary base-pairings of adenine-thymine and cytosine-guanine based on specific hydrogen bonding, and carries genetic information. Due to the stacking interaction of p-electrons at the ground states of the base-pairs, DNA can act as a p-electron medium for the photoinduced electron transfer. Toward the single molecule detection of DNA by using a novel optical technique based on the combination of near field optics and photoinduced electron transfer, polynucleic acids have been immobilized as two-dimensional molecular assemblies or single molecule by means of the specific intermolecular interaction at the air-water interface. DNA, having phosphate groups in its backbone, was assembled with counter charged amphiphiles and fixed as a polyion complex monolayer at the air-water interface. We have investigated the effect of DNA on the fluorescence lifetime of a binary component monolayer of an acridine amphiphile and a cat … More ionic double chain amphiphile. Strong fluorescence quenching was found when polyG polyC was dissolved in a water subphase. Static quenching of acridine fluorescence, probably due to the electron transfer from guanine base, is occurred because the fluorescence life-time is not so reduced as fluorescence intensity. The photoinduced electron transfer to an ITO electrode was measured by an electrochemical method. Anodic photosensitized current of the amphiphilic acridine dye is enhanced by the complex formation with polynucleic acids. PolyG polyC, whose guanine base is assumed to be *idized by the excited state of the acridine moiety, is most effective for photocurrent generation. By using the simple casting method we have prepared mesoscopic stripe pattern of DNA on a mica substrate. An aqueous DNA solution mixed with/without an aqueous alginic acid solution was dropped onto a fleshly cleaved mica surface and dried by heating up to ca. 80゚C.The mesoscopic lines parallel to the receding direction of the solvent were observed in the cast film. When alginic acid were added in the aqueous DNA solution, more regularly aligned stripe patterns were obtained. Height of those lines was below 10 nm. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] M.Shimomura 他: "Mesoscopic Pattern Formation of Nanostructured Polymer Assemblies." Supramolecular Science. 5. 331-336 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura 他: "Construction of two-dimensional DNA-mimetics. Supramolecular Architecture with Highly Stacked Base-Pairs of Amphiphilic Adenine and Thymine at the Air-Water Interface." Thin Solid Films. 327-329. 603-606 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura 他: "Mesoscopic Patterns of Molecular Aggregates on Solid Substrates." Thin Solid Films,. 327-329. 854-856 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura 他: "Formation of Ordered Mesoscopic Patterns in Polymer Cast Films by Dewetting." Thin Solid Films. 327-329. 829-832 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura 他: "Photonic and Electronic Application of Mesoscopic Polymer Assemblies." Molecular Crystal Liquid Crystal. 322. 305-312 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Okahata 他: "Anisotropic Electric Conductivity in an Aligned DNA Cast Film." Journal of American Chemical Society. 120. 6165-6166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura, et.al.: "Mesoscopic Pattern Formation of Nanostructured Polymer Assemblies." Supramolecular Science. 5. 331-336 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura, et.al.: "Construction of two-dimensional DNA-mimetics. Supramolecular Architecture with Highly Stacked Base-Pairs of Amphiphilic Adenine and Thymine at the Air-Water Interface." Thin Solid Films. 327-329. 603-606 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura, et.al.: "Mesoscopic Patterns of Molecular Aggregates on Solid Substrates." Thin Solid Films. 327-329. 854-856 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura, et.al.: "Formation of Ordered Mesoscopic Patterns in Polymer Cast Films by Dewetting." Thin Solid Films. 327-329. 829-832 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura, et.al.: "Photonic and Electronic Application of Mesoscopic Polymer Assemblies." Molecular Crystal Liquid Crystal. 322. 305-312 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Okahata et.al.: "Anisotropic Electric Conductivity in an Aligned DNA Cast Film." Journal of American Chemical Society. 120. 6165-6166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shimomura他: "Mesoscopic Pattern Formation of Nanostrucutured Polymer Assemblies" Supramolecular Science. 5. 331-336 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Shimomura他: "Construction of two-dimensional DNA-mimetics. Supramolecular Architecture with Highly Stacked Base-Pairs of Amphiphilic Adenine and Thymine at the Air-Water Interface." Thin Solid Films. 327-329. 603-606 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Shimomura他: "Mesoscopic Patterns of Molecular Aggregates on Solid Substracts." Thin Solid Films. 327-329. 854-856 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Shimomura他: "Formation of Ordered Mesoscopic Patterns in Polymer Cast Films by Dewetting." Thin Solid Films. 327-329. 829-832 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Shimomura他: "Photomic and Electronic Application of Mesoscopic Polymer Assemblies." Molecular Crystal Liquid Crystal. 322. 305-312 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Okahata他: "Anisotropic Electric Conductivity in an Aligned DNA Cast Film." Journal of American Chemical Society. 120. 6165-6166 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Shimomura, 他: "Two-Dimensional DNA-Mimetic Molecular Organizations at the Air-Water Interface." Journal of American Chemical Society. 119. 2341-2342 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Shimomura, 他: "Mesoscopic Patterns of DNA-Amphiphile Complexes." Reports on Progress in Polymer Physics in Japan. 40. 523-524 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Maruyama, M.Shimomura, 他: "Mesoscopic Pattern Formation of Nanostructured Polymer Assemblies." Supramolecular Science. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] F.Nakamura, M.Shimomura, 他: "Construction of two-dimensional DNA-mimetics.Supramolecular Architecture with Highly Stacked Base-Pairs of Amphiphilic Adenine and Thymine at the Air-Water Interface." Thin Solid Films. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Maruyama, M.Shimomura, 他: "2-D Patterns of Molecular Aggregates on Solid Substrates" Thin Solid Films. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] O.Karthaus, M.Shimomura, 他: "Formation of Ordered Mesoscopic Patterns in Polymer Cast Films by Dewetting" Thin Solid Films. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2021-04-07  

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