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Elucidation the mechanism of the immunological changes during anesthesia and abdominal surgery

Research Project

Project/Area Number 09307038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

HOSOKAWA Toyoshi  Kyoto Prefectural University of Medicine, Department of Anesthesiology, Associate Professor, 医学部, 助教授 (80165555)

Co-Investigator(Kenkyū-buntansha) HIROSE Munetake  Kyoto Prefectural University of Medicine, Department of Anesthesiology, Assistan, 医学部, 助手 (50275228)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥27,100,000 (Direct Cost: ¥27,100,000)
Fiscal Year 1998: ¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 1997: ¥16,600,000 (Direct Cost: ¥16,600,000)
Keywordsimmune / anesthesia / surgical stress / stress / sympathetic nerve system / サイトカイン / Tリンパ球 / ヘルペ-Tリンパ球
Research Abstract

One of the main purpose of this study was to develop the new method to analyze cytokine development pattern of helper T lymphocyte and natural killer cell. The challenging new method was following,
1) Extract helper T lymphocyte and natural killer cell from subpopulations of mononuclear cell directly utilizing flow cytometry.
2) Count the cell number of developing cytokine from subpopulations of mononuclear cell by ELIS POT method.
3) Quantification of the differentiation to Th1 or Th2 and the change of the developmental ratio of the cytokines.
The results were following,
1 )We could not develop the methods to gain the complete reproducibility with flow cytometry utilizing double stain with LAG-3 and CD3O.
2)The method to evaluate the change of cytokine development such as TNFgamma and lL-4 was not enough to catch the delicate change of helper T lymphocuytes.
3)We are developing the new method to evaluate the change of helper T lymphocuytes utilizimg another markers such as TNFbeta, IL-5, IL-1O,IL-13 with cd4. The method has not be established yet but we will soon succeed in the develop of the method.
4)To evaluate the change of the rates of gammadelta-T cell which is quite important as responding cell and participate to apotosis depended on the development of heat shock protein, we used the flow cytometry by the double stain with CD3 and TCR gamma/delta.
5)The change of the rates of gammadelta-T cell in the peripheral blood during upper abdominal surgery was recognize.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 廣瀬宗孝: "Heart Rate Variability during Chemical Thoracic Sympathectomy" Anesthesrology. 89. 666-670 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 細川豊史: "悪性疾患開腹術の周術期における尿中トリプシンインヒビター濃度の変動とウリナスタチン投与の影響" THERAPEVTIC RESEARCH. 19. 8-13 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 廣瀬宗孝: "Extradural buprenorphine suppresses breast feeding after Caesarean section" BRITISH JOURNAL OF ANAESTHESIA. 79. 120-121 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 細川豊史: "Does stellate ganglion block intluence immune system ?" Management of pain. 3. 31-365 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 細川豊史: "疾病とサイトカイン-病因から治療への応用まで" 真興交易医書出版部, 271 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 細川豊史: "麻酔科医のための生理学" 真興交易医書出版部, 300 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyoshi HOSOKAWA: "The Effect of Urinastatin and the Change of the Concentration of urinary trypsin inhibitor during abdominal surgery" THERAPEUTIC RESEARCH. 19 (11). 8-13 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyoshi HOSOKAWA: "Monduzzi Editore" Management of Pain a World Perspective. 517 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Munetaka HIROSE: "Extradural buprenorphine suppresses breast feeding after Caesarean section" BRISTISH JOURNAL OF ANAESTHESIA. 79 (1). 120-121 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Munetaka HIROSE: "Heart Rate Variability during Chemical Thoracic Syspathectomy" ANESTHESIOLOGY. 89 (3). 666-670 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyoshi HOSOKAWA: Disease and Cytokine. Shinkou-Koueki, 271 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyoshi HOSOKAWA: Physiology for Anesthetist. Shinkou-Koueki, 300 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 廣瀬 宗孝: "Heart Rate Variability during Chemical Thoracic Sympathectomy" Anesthesiology. 89・3. 666-670 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 細川 豊史: "悪性疾患開腹術の同術期における尿中トリプシンインヒビター濃度の変動とウリナスタチン投与の影響" THERAPEUTIC RESEARCH. 19・11. 8-13 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 廣瀬 宗孝: "Extradural buprenorphine suppresses brast feeding after Caesarean section" BRITISH JOURNAL OF ANAESTHESIA. 79・1. 120-121 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 細川 豊史: "Does stellate ganglion block influence immune system?" Management of Pain. 3. 361-365 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 細川 豊史: "疾病とサイトカイン-病因から治療への応用まで-" 真興交易医書出版部, 271 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 細川 豊史: "麻酔科医のための生理学" 真興交易医書出版部, 300 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 広瀬宗孝, 他: "Extra dural buprenorphine suppresses breust feeding after caesarean section" British Journal of Anaesthesia. 79(1). 120-121 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小田洋子, 他: "The role of spleen,plusma cortisol,emhthelin,and cytokines response during and after surgical stress" Acta Anaesthesiologica Scandinavia. 42(7)(未定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 細川豊史: "麻酔と免疫" 臨床麻酔. 22(3)(未定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 細川豊史: "疾病とサイトカイン-病因から治療への応用まで-,「麻酔とサイトカイン」" 真興交易医書出版部,編集:浅野茂隆, 谷憲三郎, 271 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 細川豊史: "基礎麻酔学セミナー" 近畿麻酔科医会, 353 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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