| Project/Area Number |
09307040
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
INOMATA Hajime Graduate School of Medical Sciences KYUSHU UNIVERSITY Professor, 大学院・医学研究院, 教授 (30038674)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Taiji Faculty of Medicine KYUSHU UNIVERSITY Assistant, 医学部・附属病院, 助手 (10235179)
ISHIBASHI Tatsuro Graduate School Medical Sciences KYUSHU UNIVERSITY Associate Professor, 大学院・医学研究院, 助教授 (30150428)
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| Project Period (FY) |
1997 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥34,800,000 (Direct Cost: ¥34,800,000)
Fiscal Year 2000: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1997: ¥22,000,000 (Direct Cost: ¥22,000,000)
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| Keywords | Neovascularization / Malignant Melanoma / Adenovirus Vector / Subretinal Neovascularization / Wound Healing / Tissue Plasminogen Activator / Transforming Growth Factor-β / Vascular Endothelial Growth Factor / トランスフォーミング増殖因子TGF-b / 糖尿病網膜症 / 遺伝子治療 / 組織因子 / 電気パルス / ウイルスベクター / TGF-β / アドレノメジュリン / 眼内血管新生 / 角膜血管新生 / MCP-I / VEGF / アデノウィルスベクター / 低酸素 / 転写因子 / 網膜血管内皮細胞 / 遺伝子導入 / IL-8 / ras inhibior |
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| Research Abstract |
We examined the effect of adenovirus-mediated gene transfer of a soluble receptor of vascular endothelial growth factor (VEGF) on the growth of experimental eyelid malignant melanoma, and it was found that the VEGF receptor (flt-1) gene inhibited the growth of the tumor. However, it caused the severe skin ulcer. Similarly, experimental subretinal neovascularization was inhibited by adenovirus-mediated soluble VEGF/flt-1 receptor gene transfection : as a role of VEGF and possible treatment for SRN in age-related macular degeneration. However, severe damages occurred in the sensory retina.
From these experimental studies, it became clear that neovascularization is rather important and indispensable phenomenon for wound healing of the tissues and organs. Therefore, it seems to be more important to inhibit the inflammation and/or tissue fibrosis during the wound healing.
To have the ideal wound healing, we prepared adenovirus vector to inhibit tissue plasminogen activator and transforming growth factor-β (TGF-β). Our results demonstrated that TGF-β indeed plays a critical role in the process of corneal opacification, edema and angiogenesis, and that adenovirus-mediated expression of a soluble TGF-β receptor can be therapeutically useful.
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