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Study of biomolecular complex formation by heteronuclear multidimensional NMR

Research Project

Project/Area Number 09307054
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 医薬分子機能学
Research InstitutionOsaka University

Principal Investigator

KOBAYASHI Yuji  Graduate sxhool of pharmaceutical sciences, Osaka university, Professor, 薬学研究科, 教授 (20127228)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥24,800,000 (Direct Cost: ¥24,800,000)
Fiscal Year 1998: ¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 1997: ¥15,000,000 (Direct Cost: ¥15,000,000)
Keywordscomplex / BMP / receptor / NMR / tertiary structure / BMPレセプター / 構造活性相関 / 複合体形成 / 蛋白質複合体 / IGF
Research Abstract

Many biomolecules form non-covalent complexes (super molecules) when they are biologically active. In the case of molecules that act in signaling systems, the formation of the non-covalent complex is the initial event that triggers some important biological process. Although many studies have been made on such complexes, little is known about the detailed mechanism of signaling. The purpose of this research project is to explore the structure and dynamics of such protein-protein complexes by multidimensional NMR techniques. Moreover, we also intend to develop physico-chemical methodologies, which are based on sedimentation equilibrium measurements, differential scanning calorimetry and surface plasmon resonance, to investigate the mechanism of complex formation. In particular, we are focusing our attention on the BMP(bone morphogenetic protein) system. We have established a bacterial expression system for BMP and its receptor molecules. The purified recombinant BMP receptor showed high binding affinity, which was analyzed quantitatively by several physico- chemical methods. Site directed mutagenesis studies have revealed that certain amino acid residues of the BMP receptor contribute to complex formation. Using heteronuclear multidimensional NMR methods, the assignments of the BMP receptor are complete and the solution structure of BMP receptor nearly determined. In other words, we have powerful tools and methods to investigate the BMP-BMP receptor complex at high (atomic) resolution.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Chino, N.: "Topological Isomers of Human Uroguanylin : Interconversion between Biologically Active and Inactive Isomers." FEBS Lett.421. 27-31 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aumelas, A.: "Formation of Native Disulfide Bonds in Endothelin-1 Structural Evidence for the Involvement of a Highly Specific Salt Bridge between the Prosequence and the Endothelin-1 Sequence." Biochemistry. 37. 5220-5230 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hasegawa, J.: "Solution Structure of Thermostable Cytochrome c-552 from Hydrogenobacter thermophilus Determined by ^1H-NMR Spectroscopy." Biochemistry. 37. 9641-9649 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, S.: "The Role of Calcium-binding Sites in S-modulin Function" J.Biol.Chem.273. 20223-20227 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamaoki, H.: "Folding Motifs Induced and Stabilized by Distinct Cystine Frameworks." Protein Engineering. 11. 649-659 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogawa, K.: "Conformation analysis of eel Calcitonin Comparison with the Conformation of Elcatonin." Eur.J.Biochem.257. 331-336 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chino, N.: "Topological Isomers of Human Uroguanylin : Interconversion between Biologically Active and Inactive Isomers" FEBS Lett.421. 27-31 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aumelas, A.: "Formation of Native Disulfide Bonds in Endothelin-1. Structural Evidence for the Involvement of a Highly sp ecific salt Bridge between the Prosequence and the Endothelin-1 Sequence." Biochemistry. 37. 5220-5230 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hasegawa, J.: "Sokltion Structure of Thermostable Cytochrome c-552 from Hydrogenobacter thermophilus Determined by ^1H-NMR Spectroscopy." Biochemistry. 37. 9641-9649 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, S.: "The Role of Calcium-binding Sites in S-modulin Function" J.Biol.Chem.273. 20223-20227 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamaoki, H.: "Folding M otifs Induced and Stabilized by Distinct Cystine Frameworks." Protein Engineering. 11. 649-659 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogawa, K.: "Conformation analysis of eel Calcitonin Comparison with the Conformation of Elcatonin" Eur.J.Biochem.257. 331-336 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Uchiyama,S.: "Measurement of Thermodynamic Quantities in the Heating-Rate Dependent Thermal Transitions of Sequenced Polytripeptides." Chem.Phys.Lett.281. 92-96 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kai,T.: "NMR Observation of Ttwo States of Triple Helix in the Thermal transition of(Pro-Pro-Gly)10." Chem.Phys.Lett.281. 86-91 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hasegawa,J.: "Solution Structure of Thermostable Cytochrome c-552 from Hydrogenobacter thermophilus Determined by H-NMR Spectroscopy." Biochemistry. 37. 9641-9649 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tamaoki,H.: "Folding Motifs Induced and Stabilized by Distinet Cystine Frameworks." Protein Engineering. 11. 649-659 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ogawa,K,: "Conformation analysis of eel Calcitonin Comparison with the Conformation of Elcatonin," Eur.J.Biochem. 257. 331-336 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroaki Gouda: "Solution structure of α-conotoxin MI determined by ^1H-NMR spectroscopy and molecular dynamics simulation with the explicit solvent water" BIOCHIMICA ET BIOPHYSICA ACTA. 1343. 327-334 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.TaKashima: "Solution Structure of Neocarzinostatin Determined by Homonuclear Two-Dimensional Nuclear Magnetic Resonance." Neocarzinostatin:The Past,Present,and Future Anticancer Drug. 83-107 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Uchiyama: "Measurement of thermodynamic quantities in the heating-rate dependent thermal transitions of sequenced polytripeptides" Chemical.Physics Letters. 281. 92-96 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naoyoshi Chino: "Topological isomers of human urogiuanylin:interconversion between biologcally active and inactive isomers" FEBS Letters. 421. 27-31 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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