| Project/Area Number |
09308025
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
HAKOSHIMA Toshio Department of Molecular Biology, Nara Institute of Science and Technology, Professor, バイオサイエンス研究科, 教授 (00164773)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Toshiyuki Department of Molecular Biology, Nara Institute of Science, Research Associate, バイオサイエンス研究科, 助手 (30273858)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥33,300,000 (Direct Cost: ¥33,300,000)
Fiscal Year 1999: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1998: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥21,600,000 (Direct Cost: ¥21,600,000)
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| Keywords | transcription / signal transduction / G protein / protein / DNA / molecular complex / X-ray / structural biology / 転写因子 / G蛋白質 / 二成分系 |
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| Research Abstract |
Crystal structures of three transcription factor/DNA complexes, PHO4/DNA, IRF-2/DNA and Pap1/DNA, were determined. Based on these structures, molecular mechanisms of base recognition by these proteins were elucidated. In the mammalian signaling pathways, crystal structures of the small G protein RhoA in the GTPγS-MgィイD12+ィエD1 bound from and its complex with the effector protein PKN were determined. The structures reveals Rho-specific switching mechanisms and its recognition of the effector protein. Moreover, crystal structures of the thioredoxin-peroxidase HBP23 and a hippocampal serine protease, neuropsin, were determined. In the bacterial His-Asp phosphorelay signaling pathways, crystal structures of the HPt domain of a sensor kinase ArcB and its protein phosphatase SixA were determined.
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