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Studies on photosynthetic mechanism of bacteria having Zn-bacteriochlorophyll

Research Project

Project/Area Number 09309008
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionTokyo Metropolitan University

Principal Investigator

SHIMADA Keizo  Tokyo Metropolitan Univ., Dept. Biol., Professor, 理学研究科, 教授 (80112473)

Co-Investigator(Kenkyū-buntansha) HIRAISHI Akira  Toyohashi Univ., Technol., Dept. Ecol Engneering, Professor, エコロジー工学系, 教授 (40283486)
TAKAMIYA Ken-ichiro  Tokyo Inst. Technol., Dept. Biological Science, Professor, 生命理工学研究科, 教授 (80037259)
ITOH Sigeru  Univ. Nagoya, Dept. Phys., Professor, 理学研究科, 教授 (40108634)
WAKAO Norio  Iwate Univ., Dept. Biosci. Technol., Prorfessor, 農学部, 教授 (30003784)
MIMURO Mamoru  Yamaguchi Univ., Dept. Physics, Biol. Informatics, Professor, 理学部, 教授 (40142004)
小林 正美  筑波大学, 物質工学系, 講師 (70234846)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥24,600,000 (Direct Cost: ¥24,600,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1997: ¥15,400,000 (Direct Cost: ¥15,400,000)
KeywordsBactriochlorophyll / Zinc / Photosynthetic bacteria / Acidophile / Reaction center / Acidiphilium / スペシャルペア / クロロフィル生合成系 / 光捕集系 / クロロフィル生合成
Research Abstract

Major results obtained during the term of this project are summarized as follows :
1. Reaction center (RC) and the sole antenna complex, LH 1, were isolated from Acidiphilium (A.) rubrum, a representative species in the Zn-bacteriochlorophyll (Zn-BChl) a producing bacteria. The RC preparation contained Zn-BChl a, bacteriopheophytin and spirilloxanthin in a ratio of 4 : 2 : 1 but no Mg-BChl a, indicating that Zn-BChl a is functioning as the special pair and the accessary BChl.
2. Genes for the RC and the LH proteins were found to form a cluster similar to the puf-operon of purple photosynthetic bacteria. Amino acid residues for cofactor binding in the purple bacterial RC were all conserved but the L168His residue which is hydrogen bonded to a side chain of one of the special pair BChl. The L168 residue was Glu in A.rubrum. A weaker interaction between the special pair BChls than that in purple bacterial RC was suggested by analysis of absorption and CD spectra which was in good agreement … More with the substitution of L168His to Glu residue.
3. Genes for proteins functioning in metal chelation in the BChl biosynthetic pathway were cloned, and the products were shown to have Mg-chelating activity by functional complimentation. The temporary incorporation of Mg atom into precursors of BChl biosynthesis was confirmed by the detection of Mg-protoporphylin IX monomethylester in A.rubrum cells. Thus, Mg in the protoporphyri ring was shown to be substituted by Zn atom at the later step (s) in the BChl biosynthesis pathway.
4. The ratio of Zn-BChl a to Mg-BChl a was around 13 : 2 under pH lower than 4, but was decreased at higher pH.The cellular BChl content was increased under starved conditions. Zn^<2+> concentration of μM order was enough for Zn-BChl formation ; Higher concentration did not enhance Zn-BChl production.
5. Zn-BChl was found only from species belonging to genus Acidiphilium. An acidophilic BChl-containing bacteria, Acidisphaera rubrifaciens found by this project could synthesize trace amount of Zn-BChl a in addition to Mg-BChl a when mM order of Zn^<2+> was provided.
6. Zn-BChl a was shown to be stable under pH conditions higher than 1 , whitle Mg was released from Mg-BChl a below pH 4. The acid-resistence of Zn-BChl a was in good agreement with the extremely acidophilic nature of Acidiphilium. Less

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] 永島賢治 ほか: "Nucleotide sequences of genes coding for photosynthetic reaction centers and light-harvesting proteins of Acidiphilium rubrum and related aerobic acidophilic bacteria."Plant & Cell Physiology. 38. 1249-1258 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 伊藤繁 ほか: "Accumulation of Fe, Cr and Ni metals inside cells of acidophilic bacterium, Acidiphilium rubrum, that produced Zn containing bacteriochlorophyll α."Plant & Cell Physiology. 39. 740-744 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 小林正美 ほか: "Acid resistance of Zn-bacteriochlorophyll a from an Acidophillic bacterium, Acidiphilium rubrum."Analitical Sciences. 14. 1149-1152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 増田建 ほか: "Magnesium insertion by magnesium chelatase in the biosynthesis of zinc bacteriochlorophyll α in an aerobic acidophilic bacterium Acidiphilium rubrum."Journal of Biological Chemistry. 274. 33594-33600 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 三室守 ほか: "Magnetic circular dichroism (MCD) properties of reaction center complexes isolated from the Zn-Bacteriochlorophyll a containing purple bacterium, Acidiphilium rubrum."Biochemistry. 39. 4020-4027 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 平石明 ほか: International Journal of Systematic and Evolutionary Microbiology. 50. 1539-1546 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nagashima, K.V.P.et al.: "Nucleotide sequences of genes coding for photosynthetic reaction centers and light-harvesting proteins of Acidiphilium rubrum and related aerobic acidophilic bacteria."Plant & Cell Physiology. vol.38. 1249-1258 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Itoh, S.et al.: "Accumulation of Fe, Cr and Ni metals inside cells of acidophilic bacterium, Acidiphilium rubrum, that produced Zn containing bacteriochlorophyll a."Plant & Cell Physiology. vol.39. 740-744 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiraishi, A.et al.: "Phylogeny and photosynthetic features of Thiobacillus acidophilus and related acidophilic bacteria : its transfer to the genus Acidiphilium as Acidiphilium acidophilum comb. nov."International Journal of Systematic Bacteriology. vol.64. 1389-1398 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kobayashi M.et al.: "Acid resistance of Zn-bacteriochlorophyll a from an Acidophillic bacterium Acidiphilium rubrum."Analytical Sciences. vol.14. 1149-1152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kobayashi, M.et al.: "Structural determination of the novel Zn-containing bacteriochlorophyll in Acidiphilium rubrum."Photomedicine and Photobiology. vol.20. 75-80 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akiyama, M.et al.: "Pigment composition of the reaction center complex isolated from an acidophilic bacterium Acidiphilium rubrum grown at pH 3.5."Photomedicine and Photobiology. vol.20. 85-87 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaichi, S.et al.: "Nomenclature of metal-substituted (bacteria) chlorophylls in natural photosynthesis : Metal-(bacterio) chlorophyll and M-(B) Chl."Photosynthesis Research. vol.59. 255-256 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masuda, T.et al.: "Magnesium insertion by magnesium chelatase in the biosynthesis of zinc bacteriochlorophyll a in an aerobic acidophilic bacterium Acidiphilium rubrum."Journal of Biological Chemistry. vol.274. 33594-33600 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kobayashi, M.: "Comparison of physicochemical properties of metallobacteriochlorophylls and metallochlorophylls."Zeitschrift fur Physikalishce Chemie. vol.213. 207-214 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akiyama, M.et al.: "Accumulation of poly-β-hydroxybutyrate in an acidophilic photosyntheti purple bacterium Acidiphilium rubrum."Photomedicine and Photobiology. vol.21. 101-104 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mimuro, M.et al.: "Magnetic cwrcular dichroism (MCD) properties of reaction center complexes isolated from the Zn-Bacteriochlorophyll a containing purple bacterium, Acidiphilium rubrum."Biochemistry. vol.39. 4020-4027 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuzawa, Y.et al.: "Ultrastructure of the acidophilic aerobic photosynthetic bacterium Acidiphilium rubrum."Current Microbiology. vol.40. 398-401 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiraishi, A.: "Acidisphaera rubrifaciens gen. nov., sp. nov., an aerobic photosynthetic bacterium isolated from acidic environments."International Journal of Systematic and Evolutionary Microbiology. vol.50. 1539-1546 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 三室守 ほか: "Magnetic circular dichroism (MCD) properties of reaction center complexes isolated from the Zn-Bacteriochlorophyll a containing purple bacterium. Acidiphilium rubrum"Biochemistry. 39. 4020-4027 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 平石明 ほか: "Ultrastructure of the acidophilic aerobic photosynthetic bacterium Acidiphilium rubrum."Current Microbiology. 40. 398-401 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 平石明 ほか: "Acidisphaera rubrifaciens gen.nov., sp.nov., an aerobic photosynthetic bacterium isolated from acidic environments"International Journal of Systematic and Evolutionary Microbiology. 50. 1539-1546 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 三室守 ほか: "植物生理学 光合成(佐藤公行 編集)「光合成色素系-光エネルギーの捕集」"朝倉書店(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 増田 建 ほか: "Magnesium insertion by magnesium chelatase in the biosynthesis of zinc bacteriochlorophyllα in an aerobic acidophilic bacterium Acidiphilium rubrum"Journal of Biological Chemistry. 274. 33594-33600 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高市真一 ほか: "Nomenclature of metal-substituted(bacterio) chlorophylls in natural photosynthesis: Metal-( bacterio) chlorophyll and M( B ) Chel."Photosynthesis Research. 59. 255-256 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 小林正美 ほか: "Comparison of physicochemical properties of metallochlorophylls and metallobacteriochlorophylls."Zeitschrift Physikalische Chemie. 213. 207-214 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三室 守 ほか: "Magnetic circular dichroism (MCD) properties of reaction center complexes isolated from the Zn-Bacteriochlorophyll a containing purple bacterium, Acidiphilium rubrum."Biochemistry. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松澤有希子 ほか: "Ultrastructure of the acidophilic aerobic photosynthetic bacterium Acidiphilium rubrum."Curret Microbiology. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 平石 明 ほか: "Acidisphaera rubrifaciens gen. nov., sp. nov., an aerobic photosynthetic bacterium isolated from acidic environments"International Journal of Systematic and Evolutionary Microbiology. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三室 守: "「光合成色素系-光エネルギーの捕集」植物生理学 光合成"朝倉書店(佐藤 公行 編集) (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 伊藤 繁 ほか: "Accumulation of Fe,Cr and Ni metals inside cells of acidophilic bacterium Acidiphilium rubrum that produced Zn containing bacteriochlorophyll a." Plant and Cell Physiology. 39. 740-744 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 平石 明 ほか: "Phylogeny and photosynthetic features of Thiobacillus acidophilus and related acidophilic bacteria: its transfer to the genus Acidiphilium as Acidiphilium acidophilum comb.nov." International Journal of Systematic Bacteriology. 64. 1389-1398 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 高市真一 ほか: "Nomenclature of metal-substituted (bacterio)chlorophylls in natural photosynthesis:metal (bacterio)chlorophyll and M-(B)Chl" Photosynthesis Research. 59 印刷中. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小林正美 ほか: "Acid resistance of Zn-bacteriochlorophyll a from an acidophilic bacterium Acidiphilium rubrum" Analytical Science. 14. 1149-1152 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小林正美 ほか: "Successfully controlled isomerization and pheophytinization of bacteriochlorophyll b by weak acid in the dark in vitro" Analytica Chimica Acta. 361. 285-290 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小林正美 ほか: "Light-independent isomerization of bacteriochlorophyll g to chlorophyll a catalyzed by weak acid in vitro" Analytica Chimica Acta. 365. 199-203 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 永島賢治 ほか: "Nucleotide sequences of genes coding for photosynthetic reaction centers and light-harvesting proteins of Acidiphilium rubrum and related aerobic acidophilic bacteria." Plant and Cell Physiology. 38. 1249-1258 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 伊藤 繁 ほか: "Accumulation of Fe,Cr and Ni metals inside cells of acidophilic bacterium Acidiphilium rubrum that produces Zn containing bacteriochlorophyll a." Plant and Cell Physiology. 39(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林 正美 ほか: "Successfully controlled isomerization and pheophytinization of bacteriochlorophyll b by weak acid in the dark in vitro" Analytica Chimlca Acta. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林 正美 ほか: "Light-independent isomerization of bacteriochlorophyll g to chlorophyll a catalyzed by weak acid in vitro" Analytica Chimica Acta. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林 正美 ほか: "クロロフィルの中心金属はマグネシウムだけか?" 鉱物学会誌. 26(2). 117-120 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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