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Development of advanced purification system using high performance specific microorganisms for water enviromment restoration

Research Project

Project/Area Number 09355017
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Civil and environmental engineering
Research InstitutionNational Institute For Environmental Studies

Principal Investigator

INAMORI Yuhei  National Institute for Environmental Studies, Regional Environment Division, Executive Researcher, 地域環境研究グループ, 総合研究官 (10142093)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Rikiya  Tokyo Univ. of Agriculture, Professor, 応用生物科学部, 教授 (20154883)
WATANABE Makoto  National Institute for Environmental Studies, Environment Biology Division, Head, 生物圏環境部, 部長 (10132870)
MIZUOCHI Motoyuki  National Institute for Environmental Studies, Regional Environment Division, Senior Researcher, 地域環境研究グループ, 主任研究員 (50260188)
HAYASHI Norio  Natural History Museum and Institute, Chiba Curator, 環境科学研究科, 上席研究員 (60250156)
MATSUMURA Masatoshi  Univ. of Tsukuba. Professor, 応用生物化学系, 教授 (50015781)
須藤 隆一  東北大学, 大学院・工学研究科, 教授 (70109916)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥24,500,000 (Direct Cost: ¥24,500,000)
Fiscal Year 2000: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1999: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1998: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1997: ¥7,600,000 (Direct Cost: ¥7,600,000)
Keywordsspecific microorganisms / mass culture / environmenal factor / spore / inoculation / wastewater treatment / 定着化 / 排水処理 / 増殖特性 / 原生動物 / 膜口類 / 収率 / 汚泥減量 / 輪虫類 / 縁毛類 / 硝化細菌 / FISH法 / 輪虫 / モノクローナル抗体 / 汚泥減量化 / 窒素除去 / 貧毛類 / 水環境修復 / 機能強化
Research Abstract

The focal point of this research is how to make spore of specific microorganisms, and clarify the practical use methods. Thus. the methods of mass culture about specific microorganisms were developed. Some specific microorganisms such as nitrification bacteria (Genus Nitrobacter and Genus Nitorosomonas), Protozoa Colpidium campylum, Tetrahymena Pyriformis, Vorticella microstoma, and Vorticella convallaria, Rotifer Philodina erythrophthalma, Rotaria rotatoria, Lecane luna, Oljgochaeta Aeolosoma hemprichi, Nais variabils, Pristina longiseta and so on were selected in this study. The effects of some environmental factors on these specific microorganisms were tested to know the response of these specific microorganisms against various kind of environmental factors such as water temperature, PH, phosphate buffer concentration. dissolved oxygen concentration qualities and quantities of food, shake stress and so forth. These results let us know the best way for mass cultivation of these specific microorganisms. Furthermore, the relationship among these specific microorganisms and various kind of common microorganisms that appearing in wastewater treatment facilities through model ecosystem investigations was clarified. On the other hand the best method to make spore of these specific microorganisms was studied. The germination method from these spores was clarified. In addition. polyethylene glycol(PEG). polyvinyl alcohol(PVA) and cellose were compared as mass specific microorganisms keeper in practical use for analysis of physical strength, specific surface area, activity maintaining ratio. The basic information about advaced technology on utilization of specific microorganisms was obtained.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] 高井智丈: "生物処理施設における硝化細菌の迅速かつ簡便な検出・定量化手法の開発"水環境学会誌. 20(5). 318-323 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Inamori: "Development of a Rapid Quantification Method for Nitrosomonas and Nitrobacter Using ELISA for Wastewater Treatment"Water Science and Technology. 36(12). 169-174 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 国安克彦: "環形動物貧毛類の増殖に及ぼす環境因子の影響"日本水処理生物学会誌. 33(4). 207-214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 林紀男: "袋形動物輪虫類の増殖に及ぼす環境因子の影響"日本水処理生物学会誌. 34(3). 205-213 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 藤井邦彦: "有用微小動物輪虫類の大量定着と水質浄化に及ぼす付着担体の効果"水環境学会誌. 22(2). 127-132 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 林紀男: "米ぬか含有物質による有用微小動物輪虫類Philodina erythrophthalmaの増殖促進効果"浄化槽研究. 11(1). 35-45 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 林紀男: "原生動物縁毛類の増殖に及ぼす環境因子の影響"日本水処理生物学会誌. 35(4). 271-278 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Fujimoto: "Effect of Metazoa on Proportion of Physiologically Active Bacteria"Japanese Journal of Water Treatment Biology. 35(4). 279-284 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Noda: "Evaluation of coexisting effect of Nitrosomonas europaea and Nitrobacter winogradski on biological nitrification by monoclonal antibody method"8th International Conference on the Conservation and Management of Lakes. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 林紀男: "生物処理における有用微小動物の収率"浄化槽研究. 11(2). 57-66 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Noda: "Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method"Water Science and Technology. 41(4-5). 301-308 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Ikuta: "The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method"Water Science and Technology. 42(3-4). 1-7 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomotake Takai: "Development of rapid detection and quantification methods for nitrifying bacteria using monoclonal antibodies"Journal of Japan Society on Water Environment. 20(5). 318-323 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yuhei Inamori: "Development of a rapid quantification method for Nitrosomonas and Nitrobacter using ELISA for wastewater treatment facilities"Water Science and Technology. 36(12). 169-174 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuhiko Kuniyasu: "Effect of environmental factors on growth characteristics of Oligochaeta"Japanese Journal of Water Treatment Biology. 33(4). 207-214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Norio Hayashi: "Effect of environmental factors on growth Characteristics of rotatoria"Japanese Journal of Water Treatment Biology. 34(3). 205-213 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kunihiko Fujii: "Effect of the packed media on the fixation of bdelloid rotifer, Philodina erythrophthalma and water purification in the wastewater treatment Process"Journal of Japan Society on Water Environment. 22(2). 127-132 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Norio Hayashi: "Growth acceleratlon efficiency on rice component to rotatoria"Journal of Domestic Wastewater Treatment Research. 11(1). 35-45 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Norio Hayashi: "Effect of environmental factors on growth characteristics of Peritrichida"Japanese Journal of Water Treatment Biology. 35(4). 271-278 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naoshi Fujimoto: "Effect of metazoa on proportion of physiologically active bacteria"Japanese Journal of Water Treatment Biology. 35(4). 279-284 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Noda: "Evaluation of coexisting effect of NItrosomonas europaea and Nirobacter winogradskyi on biological nitrification by nonoclonal antibody method"8th International Conference on the Conservation and Management of Lakes. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Norio Hayashi: "Yield of specific micro-animals contributed of the biological treatment"Journal of Domestic Wastewater Treatment Research. 11(2). 57-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Noda: "Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method"Water Science and Technology. 41(4-5). 301-308 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Ikuta: "The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method"Water Science arid Technology. 42(3-4). 1-7 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 林紀男: "生物処理における有用微小動物の収率"浄化槽研究. 11(2). 57-66 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 生田創: "The Rapid Quantification and Detection of Nitrifying Bacteria by Using Monoclonal Antibody Method"Water Science & Technology. 42(3-4). 1-7 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 野田尚宏: "Rapid Quantification and In Situ Detection of Nitrifying Bacteria in Biofilms by Monoclonal Antibody Method"Water Science & Technology. 41(4-5). 301-308 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 稲森悠平: "環境修復のための生態工学"講談社サイエンティフィク. 238 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 林紀男: "原生動物縁毛類の増殖に及ぼす環境因子の影響"日本水処理生物学会誌. 35(4). 271-278 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naoshi Fujimoto: "Effect of metazoa on proportion of physiologically active bacteria"Japanese Journal of Water Treatment Biology. 35(4). 279-284 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naohiro Noda: "Evaluation of coexisting effect of Nitrosomonas europaea and Nitrobacter winogradskyi on biological nitrification by monoclonal antibody method"8th Int.Conf.. On the Conservation and Management of Lakes. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 林紀男: "袋形動物輪虫類の増殖に及ぼす環境因子の影響" 日本水処理生物学会誌. 34(3). 205-213 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤井邦彦: "有用微小後生動物輪虫類の大量定着と水質浄化に及ぼす付着担体の効果" 水環境学会誌. 22(2). 127-132 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 林紀男: "米ぬか含有物質による有用微小動物輪虫類Philodina erythrophthalmaの増殖促進効果" 浄化槽研究. 11(1)(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 稲森悠平: "生活排水対策" 産業用水調査会, 380 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Fujii: "Environmental Condition for Producing Eggs of Rotifera Philodina Erythrophthalma" 6th IAWQ Asia-Pacific Regional Conference. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井智丈: "生物処理過程における硝化細菌の迅速かつ簡便な検出・定量化手法の開発" 水環境学会誌. 20、5. 318-323 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Inamori: "Development of Rapid Quantification Way of Nitrosomonas and Nitrobacter Using ELISA for Wastewater Treatment" Water Science and Technology. 36(12). 169-174 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 国安克彦: "環形動物貧毛類の増殖に及ぼす環境因子の影響" 日本水処理生物学会誌. (印刷中).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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