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Molecular-biological analysis of age-related markers present in body fluids and its forensic applications

Research Project

Project/Area Number 09357004
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Legal medicine
Research InstitutionGunma University School of Medicine

Principal Investigator

KISHI Koichiro  Gunma University School of Medicine, Department of Legal Medicine, Professor, 医学部, 教授 (30169841)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Tamiko  Gunma University School of Medicine, Department of Legal Medicine, Assistant, 医学部, 助手 (40008561)
TAKESHITA Haruo  Gunma University School of Medicine, Department of Legal Medicine, Lecturer, 医学部, 講師 (90292599)
YASUDA Toshihiro  Fukui Medical University, Department of Biology, Professor, 医学部, 助教授 (80175645)
YAZAWA Shin  Japan Immunoresearch Laboratory, 副所長
細見 修  群馬大学, 医学部, 助手 (30134274)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥28,300,000 (Direct Cost: ¥28,300,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1997: ¥15,200,000 (Direct Cost: ¥15,200,000)
Keywordsestimation of age / age-related markers / gene expression / cDNA cloning / Ugl-Y / Molecular-biological analysis / forensic application / body fluid / PCR / differential display / 分子生物学 / 年齢 / cDNA / 尿 / アミノ酸配列 / 鑑識科学
Research Abstract

Although individualization in legal medicine is one of very important research field, a number of age-related markers suitable for estimation of age is extremely limited. In this study, we have examined biochemical markers specific to each step of aging and develpment and tried to establish the method to estimate the age from forensic samples by biochemical and molecular-biological detection of these markers
1. Ugl-Y is a glycoprotein which has been discovered as an age-related marker detected in urine of only young subjects. In this study, we has determined its primary structure. Ugl-Y consisted of 180 amino acid residues, and from the database search, the amino acid sequence of Ugl-Y was found to be entirely identical to that of a part of fibronectin. From the result, it was assumed that Ugl-Y might be derived from a limited digested product or alternative spliced form of primary gene transcript of fibronectin.
2. We have surveyed gene expressed in the age-related manner in the mouse k … More idney to find novel age-related markers. Consequently, seven kinds of age-dependent expressed cDNA could be identified using a fluorescence differential display-PCR (FDD-PCR) technique and comparative RT-PCR technique. Therefore, these methods were found to be very useful for detecting new age-related genes expressed differentially.
3. We have elucidated molecular and biological properties of one of age-related genes described above. Its cDNA was 3,058 bp long and consisted of 194 amino acid residues encoding the 2.2 kb protein. From BLAST searching, it was clarified that this protein had high homology to peroxisomal protein, Mpv 17 and PMP 22. Therefore, we named this novel protein as M-LP belonging to Mpv 17 domain family. Moreover, its gene was 5 kb long with three exons, and age-related expression of the gene was confirmed. From these findings made in this research project, we could establish the basis for the molecular and biological approach which is indispensable for analysis and forensic application of age-related markers. Less

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (155 results)

All Other

All Publications (155 results)

  • [Publications] Toshihiro Yasuda: "Purification of deoxyribonuclease I(DNase I) using two-step chromatography"Analytical Biochemistry. 245(2). 255-257 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Aoyama Masahiro: "DYS19 marker in Japanese populations"International Journal of Legal Medicine. 110(2). 112-113 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Population studies of human deoxyribonuclease I polymorphism"Human Heredity. 47(3). 121-124 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Mouse deoxyribonuclease I (DNase I)"Biochemistry and Molecular Biology International. 42(1). 65-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Rabbit deoxyribonuclease I (DNase I)"Biochemical Journal. 325(2). 465-473 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Reiko Iida: "The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism"Electrophoresis. 18(11). 1936-1939 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "The STR loci HumTPO and HumLPL : population genetic data in eight populations"International Journal of Legal Medicine. 110(6). 331-333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osamu Hosomi: "Simple purification method of the antiphospholipid antibody from normal human plasma"Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on SYBR Green I fluorescence"Analytical Biochemistry. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Molecular cloning of the cDNA encoding human deoxyribonuclease II"The Journal of Biological Chemistry. 273(5). 2610-2616 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chaine reaction and fluorescence in situ hybridization analysis"Biochemical and Biophysical Reserach Communications. 244(3). 815-818 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis"The Japanese Journal of Legal Medicine. 52(2). 139-143 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Souichi Tsutsumi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric carcinoma"Cancer. 82(9). 1621-1625 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tamiko Nakajima: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity"Immunological Investigation. 27(3). 145-152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Structure and organization of the human deoxyribonuclease II (DNase II) gene"Annals of Human Genetics. 63(4). 299-305 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osamu Shimada: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine"The Journal of Histochemistry and Cytochemistry. 46(7). 833-840 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Reiko Iida: "Detection of isoenzymes of deoxyribonucleases I and II on electrophoresed gels with picogram sensitivity using SYBR Green I"Electrophoresis. 19(14). 299-305 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Identification of the three non-identical subunits constituting human deoxyribonuclease II"FEBS Letters. 440(1/2). 239-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Journal of Forensic Sciences. 44(1). 175-178 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Structural requirement of a human deoxyribonuclease II for the development of the active enzyme form, revealed by site-directed mutagenesis"Biochemical and Biophysical Reserach Communications. 256(3). 591-594 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osamu Hosomi: "Xenopus lavis pancreatic DNase I"Experimental and Clinical Imunogenetics. 16(2). 117-123 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "A new allele, DNASE1^*6, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemical and Biophysical Reserach Communications. 260(1). 280-283 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Simultaneous typing of the STR loci, vWF and HumTPO, using discontinuous polyacrylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimitsu Nakashima: "Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I"DNA Sequence. 11(3/4). 247-255 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tamiko Nakajima: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. 17(2). 71-76 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney fluorescence differential display-PCR analysis"Mechanisms of Aging and Development. 113(2). 133-142 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Letters. 467(1/2). 231-234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Mammalian deoxyribonuclease I are classified into three types"Biochemical and Biophysical Research Communications. 269(2). 481-484 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Souichi Tsutsumi: "Association of DNase 1 phenotype 2 with colorectal carcinoma risk in Japanese populations"Cancer Letters. 159(1). 109-112 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Geographical north-south decline in DNase 1 allele 2 in Japanese populations"Human Biology. 73(1). 129-134 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Japanese population data for two STR loci, HumTPO and HumLPL"Legal Medicine. 2(2). 64-67 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane : a case study and animal experiments"International Journal of Legal Medicine. 114(1/2). 96-100 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Activity staining for detection of ribonulceases by means of the dried agarose film overlay method after electrophoresis"Methods in Enzymology. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruo Takeshita: "A death resulting from inadvertent intravenous infusion of exteral feed"International Journal of Legal Medicine. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koichiro Kishi: "DNase I : structure, function, and use in medicine and forensic science"Legal Medicine. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 灘野大太: "アポトーシスとエンドヌクレアーゼとの関連"臨床薬理の進歩. No.18. 105-110 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 岸紘一郎: "核酸分解酵素DNase Iの遺伝的多型の発見"医学のあゆみ. 180(10). 658-659 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 飯田礼子: "PCR-SSCP法によってヒトdeoxyribonuclease I遺伝子の第7イントロン内に検出される新しい多型部位"DNA多型. Vol.5. 203-206 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 安田年博: "ヒト脳下垂体には高活性なdeoxyribonuclease Iが存在する"日本法医学雑誌. 52(2). 295-300 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 飯田礼子: "PCR-SSCP法によって検出されたdeoxyribonuclease I遺伝子の新規なミスセンス変異"DNA多型. Vol.6. 132-135 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 藤川克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 安田年博: "ヒトdeoxyribonuclease II(DNase II)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)

    • Description
      「研究成果報告書概要(和文)」より
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      2000 Final Research Report Summary
  • [Publications] 岸紘一郎: "DNase I"日本臨床. 57(増刊). 486-489 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 竹下治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. Vol.7. 88-90 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 飯田礼子: "年齢依存性を示すマウス腎の新規遺伝子検索"DNA多型. Vol.8. 123-126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 竹下治男: "DNASE1^*6遺伝子がつくる酵素の寿命は短い"DNA多型. Vol.8. 207-210 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 竹下治男: "STR座位HumTPOとHumLPL:日本人7集団のデータについて"DNA多型. Vol.9(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 飯田礼子: "マウス腎の年齢依存性発現遺伝子"DNA多型. Vol.9(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Purification of deoxyribonuclease I (DNase I) using two-step chromatography."Anal.Biochem.. 245(2). 121-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Aoyama, T.Matsuki, K.Kishi, R.Iida, E.Tsubota: "DYS19 marker in Japanese populations."Int.J.Legal Med.. 110(2). 112-113 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, I.Yuasa, K.Kishi: "Population studies of human deoxyribonuclease I polymorphism."Hum.Hered.. 47(3). 121-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Mouse deoxyribonuclease I (DNase I) : biochemical and immunological characterizations, cDNA structure and tissue distribution."Biochem.Mol.Biol.Int.. 42(1). 65-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Rabbbit deoxyribonuclease I (DNase I) : purification from urine, immunological and proteochemical characterization, nucleotide sequence, expression in tissues, relationships with other DNases I and phylogenetic analysis."Biochem.J.. 325(2). 465-473 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, M.Aoyama, E.Tsubota, M.Kobayashi, I.Yuasa, K.Kishi: "The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism."Electrophoresis. 18(11). 1936-1939 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] O.Hosomi, A.Takeya, T.Yasuda, S.Yazawa, T.Nakajima, T.Kogure, H.Iwai, K.Kishi: "Simple purification method of the antiphospholipid antibody from normal human serum."Exp.Clin.Immunogenet.. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, K.Kishi: "Genetic polymorphisms detected in human urine : their application to forensic individualization."Jpn.J.Legal Med.. 51(6). 407-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, D.Nadano, H.Takeshita, R.Iida, K.Kishi: "Anthropological applications of genetic polymorphisms detected in human urine : a review of Japanese studies."Rivista di Antropologia. 75(1). 407-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, E.Nakazato, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence."Anal.Biochem.. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Molecular cloning of the cDNA encoding human deoxyribonuclease II."J.Biol.Chem.. 273(5). 2610-1616 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, T.Nakajima, O.Hosomi, Y.Nakashima, K.Mogi, K.Kishi: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis."Biochem.Biophys.Res.Commun.. 244(3). 815-818 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, O.Hosomi, Y.Nakashima, S.Tsutsumi, K.Kishi: "Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis."Jpn.J.Legal Med.. 52(2). 139-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Tsutsumi, T.Asao, Y.Nagamachi, T.Nakajima, T.Yasuda, K.Kishi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric carcinoma."Cancer. 82(9). 1621-1625 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nakajima, T.Yasuda, Y.Nakashima, O.Hosomi, H.Takeshita, K.Kishi: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity."Immunol.Inverst.. 27(3). 145-152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, S.Tsutsumi, T.Nakajima, O.Hosomi, S.Mori, K.Kishi: "Structure and organization of the human deoxyribonuclease II (DNase II) gene."Ann.Hum.Genet.. 62(4). 299-305 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] O.Shimada, H.Ishikawa, H.Shimada, T.Yasuda, K.Kishi, S.Suzuki: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine."J.Histochem.Cytochem.. 46(7). 833-840 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, E.Tsubota, Y.Nakashima, K.Sawazaki, M.Aoyama, T.Matsuki, K.Kishi: "Detection of isoenzymes of deoxyribonucleases I and II on electrophoresed gels with picogram sensitivity using SYBR Green I."Electrophoresis. 19(14). 299-305 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, R.Iida, T.Nakajima, O.Hosomi, Y.Nakashima, S.Mori, K.Kishi: "Identification of the three non-identical subunits constituting human deoxyribonuclease II."FEBS Lett.. 440(1/2). 239-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] R.Iida, K.Sawazaki, T.Yasuda, E.Tsubota, M.Aoyama, T.Matsuki, K.Kishi: "Deoxyribonuclease I phenotyping from saliva stains."J.Forensic Sci.. 44(1). 175-178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, T.Nakajima, O.Hosomi, Y.Nakashima, S.Mori, K.Kishi: "Structural requirement of a human deoxyribonuclease II for the development of the active enzyme form, revealed by site-directed mutagenesis."Biochem.Biophys.Res.Commun.. 256(3). 591-594 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] O.Hosomi, T.Yasuda, H.Takeshita, T.Nakajima, Y.Nakashima, Y.Hanaoka, K.Kishi: "Xenopus laevis pancreatic DNase I : purification and characterization."Exp.Clin.Immunogenet.. 16(2). 117-123 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, S.Kogure, K.Kishi: "A new allele, DNASE1^*6, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability."Biochem.Biophys.Res.Commun.. 260(1). 280-283 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, Y.Nakashima, S.Mori, K.Mogi, K.Kishi: "Simultaneous typing of the STR loci, vWF and HumTPO, using discontinuous polyacrylamide gel electrophoresis."Legal Med.. 1(3). 233-237 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Nakashima, T.Yasuda, H.Takeshita, T.Nakajima, O.Hosomi, S.Mori, K.Kishi: "Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I."Int.J.Biochem.Cell Biol.. 31(11). 1313-1324 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, E.Tsubota, T.Matsuki, K.Kishi: "Age-related changes of gene expression in mouse kidney fluorescence differential display-PCR analysis."Mech.Ageing Dev.. 113(2). 135-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, E.Nakazato, T.Nakajima, Y.Nakashima, S.Mori, K.Mogi, K.Kishi: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)."FEBS Lett.. 467(1/2). 231-234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Nakajima, T.Yasuda, H.Takeshita, Y.Nakashima, S.Mori, K.Mogi, K.Kishi: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies."Exp.Clin.Immunogenet.. 17(2). 71-76 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, O.Hosomi, H.Takeshita, T.Nakajima, Y.Nakashima, S.Mori, K.Mogi, K.Kishi: "Molecular clonins of cDNA encoding Xenopus laevis deoxyribonuclease I."DNA Sequence. 11(3/4). 247-255 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, K.Mogi, T.Yasuda, T.Nakajima, S.Mori, T.Hoshino, K.Kishi: "Mammalian deoxyribonuclease I are classified into three types."Biochem.Biophys Res.Commun.. 269(2). 481-484 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, K.Mogi, T.Yasuda, S.Mori, Y.Nakashima, T.Nakajima, H.Akuzawa, S.Nakajo, Y.Hirota, K.Kishi: "Postmortem absorption of dichloromethane : a case study and animal experiments."Int.J.Legal Med.. 114(1/2). 96-100 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, S.Mori, T.Yasuda, K.Mogi, T.Nakajima, E.Nakazato, S.Tsutsumi, K.Fujikawa, Y.Kaneko, R.Iida, K.Kishi: "Japanese population data for two STR loci, HumTPO and HumLPL."Legal Med.. 2(2). 64-67 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Tsutsumi, H.Takeshita, T.Yasuda, H.Kuwano, K.Kishi: "Association of the DNase I phenotype 2 with colorectal carcinoma risk in a Japanese population."Cancer Lett.. 159(1). 109-112 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, Y.Nakashima K.Mogi, K.Kishi., H.Shiono, K.Sagisaka, I.Yuasa, H.Nishimukai, H.Kimura: "Geographical north-south decline in DNase I allele 2 in Japanese populations."Hum.Biol.. 73(1). 129-134 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, K.Kishi : Ribonucleases: "Activity staining for detection of ribonucleases using the dried agarose film overlay method after electrophoresis."Methods in Enzymology.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, S.Mori, K.Mogi, H.Ohkawara, M.Nara, K.Kishi: "A death resulting from inadvertent intravenous infusion of enteral feed."Int.J.Legal.Med.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kishi, T.Yasuda, H.Takeshita: "DNase I : structure, function, and use in medicine and forensic science."Legal Med.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Molecular cloning of cDNA encoding Xenopus iaevis deoxyribonuclease I"DNA Sequence. 11(3/4). 247-255 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tamiko Nakajima: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. 17(2). 71-76 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney fluorescence differential display-PCR analysis"Mechanisms of Aging and Development. 113(2). 133-142 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Letters. 467(1/2). 231-234 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Mammalian deoxyribonuclease I are classified into three types"Biochemical and Biophysical Research Communications. 269(2). 481-484 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Souichi Tsutsumi: "Association of DNase I phenotype 2 with colorectal carcinoma risk in a Japanese population"Cancer Letters. 159(1). 109-112 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Geographical north-south decline in DNase I allele 2 in Japanese populations"Human Biology. 73(1). 129-134 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Japanese population data for two STR loci, HumTPO and HumLPL"Legal Medicine. 2(2). 64-67 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane : a case study and animal experiments"International Journal of Legal Medicine. 114(1/2). 96-100 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Activity staining for detection of ribonulceases by means of the dried agarose film overlay method after electrophoresis"Methods in Enzymology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "A death resulting from inadvertent intravenous infusion of exteral feed"International Journal of Legal Medicine. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koichiro Kishi: "DNase I : structure, function, and use in medicine and forensic science"Legal Medicine. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 竹下治男: "DNASE1^*6遺伝子のつくる酵素の寿命は短い"DNA多型. 8巻. 207-210 (2000)

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      2000 Annual Research Report
  • [Publications] 飯田礼子: "年齢依存性発現を示すマウス腎の新規遺伝子検索"DNA多型. 8巻. 123-126 (2000)

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      2000 Annual Research Report
  • [Publications] 竹下治男: "STR座位HumTPOとHumLPL:日本人7集団のデーターについて"DNA多型. Vol.9(印刷中). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 飯田礼子: "マウス腎の年齢依存性発現遺伝子"DNA多型. Vol.9(印刷中). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Jourual of Forensic Sciences. 44(1). 175-178 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Structural requirement of human deoxyribonuclease II for the development of the active enzyme form,revealed by site-directed mutagenesis"Biochemocal and Biophysical Research Communications. 256(3). 591-594 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Hosomi: "Xenopus laevis pancreatic Dnase I:purification and characterization"Experimental and Clinical Immunogenetics. 19(2). 117-123 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "A new allele,DNASE1^*6,of human deoxyribouuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemocal and Biophysical Research Communications. 260(1). 280-283 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Simultaneous typing of the STR loci,vWF and HumTPO,using discontinuous polyacrylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimitsu Nakashima: "Molecular,biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tamiko Nakajima: "Rapid purification of human Dnase I using mouse monoclonal anti-Dnase I antibodies and characterzation of the antibodies"Experimental and Clinical Immunogenetics. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney:fluorescence differential display-PCR analysis"Mechanisms of Ageing and Development. 113(2). 113-142 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (Dnase II)"FEBS Letters. 467(1/2). 231-234 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Hosomi: "Molecular cloning of cDNA encoding Xenopus Laevis deoxyribonuclease I"DNA Sequence. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 藤川克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 安田年博: "ヒトdeoxyribonuclease II(Dnase II)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)

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      1999 Annual Research Report
  • [Publications] 岸紘一郎: "Dnase I"日本臨床. 57(増刊). 486-489 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 竹下治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. 7. 88-90 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane:a case study and animal experiments"International Journal of Legal Medicine. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Mammalian deoxyribonucleases I are classified into three types:pancreas,parotid and pancreas-parotid(mixed) types,based on differences of their tissue concentrations"Biochemical and Biophysical Reseach Communications. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Activity measurement of deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence" Anal.Biochem.255(2). 274-276 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Molecular cloning of the cDNA encoding human deoxyribonuclease II" J.Biol.Chem.273(5). 2610-2616 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Chromosomal localization of a human deoxyribonuclease II gene(DNase II)to 19p13.2-13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis" Biochem.Biophys.Res.Commun.244(3). 815-818 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Haruo Takeshita: "Detection of the two short tandem repeat loci(HumTPO and HumLPL)in Japanese populations using discontinuous polyacrlamide gel electrophoresis" Jpn.J.Legal Med.52(2). 139-143 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shoichi Tsutsumi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor for gastric carcinoma" Cancer. 82(9). 1621-1625 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tamiko Nakajima: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity" Immunol.Invest.27(3). 145-152 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Structure and organization of the human deoxyribonuclease II(DNase II)gene" Ann.Hum.Genet.62(4). 299-305 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Reiko Iida: "Detection of isozymes of deoxyribonucleases I and II on electrophoresed gels with picogram sensitivity using SYBR Green I" Electrophoresis. 19(14). 2416-2418 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osamu Shimada: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine" J.Histochem.Cytochem.46(7). 833-840 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Haruo Takeshita: "Identification of the three non-identical subunits constituting human deoxyribonuclease II" FEBS Lett.440(1/2). 239-242 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osamu Hosomi: "Xenopus laevis pancreatic DNase I: purification and characterization" Exp.Clin.Immunogenet.(in press). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains" J.Forensic Sci.44(1). 175-178 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "High activity of deoxyribonuclease I identified in human pituitary gland-structure and function-" Jpn.J.Legal Med.52(5). 295-300 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 安田年博: "ヒトdeoxyribonuclease II(DNase II)に関する最近の研究" 北関東医学. 49(1). 39-41 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 飯田礼子: "PCR-SSCP法によって検出されたヒトdeoxyribonuclease I遺伝子内の新規なミスセンス変異" DNA多型. 6. 132-135 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Structural requirements of a human deoxyribonuclease II for the development of the active enzyme form,revealed by site-directed mutagenesis" Biochem.Biophys Res.Commun.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasuda,T.: "Purification of deoxyribonuclease I (DNase I) using two-step chromatography" Analytical Biochemistry. 245(2). 255-257 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyama,M.: "DYS19 marker in Japanese populations" International Journal of Legal Medicine. 110(2). 112-113 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Populations studies of human deoxyribonuclease I polymorphism" Human Heredity. 47(3). 121-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "Mouse deoxyribonuclease I (DNase I)" Biochemistry and Molecular Biology International. 42(1). 65-75 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iida,R.: "The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism" Electrophoresis. 18(11). 1936-1939 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Rabbit deoxyribonuclease I (DNase I)" Biochemical Journal. 325(2). 465-473 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "The STR loci HumTPO and HumLPL : population genetic data in eight populations" International Journal of Legal Medicine. 110(6). 331-333 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence" Analytical Biochemistry. 255(2). 274-276 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hosomi,O.: "Simple purification method of the antiphospholipid antibody from normal human plasma" Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Genetic polymorphisms detected in human urine : their application to forensic individualization" The Japanese Journal of Legal Medicine. 51(6). 407-416 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Molecular cloning of the cDNA encoding human deoxyribonuclease II" The Journal of Biological Chemistry. 273(5). 2610-2616 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsutsumi,S.: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric cancer" Cancer. in press.

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous poiyacrylamide gel electrophoresis" The Japanese Journal of Legal Medicine. in press.

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakajima,T.: "Two novel screening methods for selecting,monoclonal antibodies which specifically inhibit DNase I enzyme activity" Immunological Investigations. in press

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis" Biochemical and Biophysical Research communications.

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Anthropological application of genetic polymorphisms detected in human urine : a review of Japanese studies" Rivista di Antropologia. in press.

    • Related Report
      1997 Annual Research Report
  • [Publications] 灘野 大太: "アポトーシスとエンドヌクレアーゼとの関連性" 臨床薬理の進歩. No.18. 105-110 (1997)

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      1997 Annual Research Report
  • [Publications] 岸 紘一郎: "核酸分解酵素DNase Iの遺伝的多型の発見" 医学のあゆみ. 180(10). 658-659 (1997)

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      1997 Annual Research Report
  • [Publications] 灘野大太: "ヒトのDNase I およびIIとアポトーシスとの関連性 : ヒト DNase I 遺伝子構造とpolymorphic site の同定" 総合健康推進財団 研究報告書(第11)平成6年度助成事業. 111-119 (1997)

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      1997 Annual Research Report
  • [Publications] 安田年博: "核酸分解酵素の遺伝的多型を利用した膵肝疾患罹患のハイリスクグループ特定に関する予防医学的研究" 膵臓病研究財団第3回研究報告書. No.3. 1-4 (1997)

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      1997 Annual Research Report
  • [Publications] 飯田礼子: "PCR-SSCP法によってヒトdeoxyribonuclease I遺伝子の第7イントロン内に検出される新しい多型部位" Vol.5. 1997. DNA多型 (203-206)

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      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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