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Design and development of anti-tumor drugs using the energy metabolism specifically held in tumor cells as potential targets

Research Project

Project/Area Number 09357020
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 医薬分子機能学
Research InstitutionUniversity of Tokushima

Principal Investigator

TERADA Hiroshi  Fac.Pharm.Sci., Univ.Tokushima, Professor, 薬学部, 教授 (00035544)

Co-Investigator(Kenkyū-buntansha) HORI Hitoshi  Fac.Eng., Univ.Tokushima, Professor, 工学部, 教授 (90119008)
YAMAZAKI Naoshi  Fac.Pharm.Sci., Univ.Tokushima, Assistant professor, 薬学部, 助手 (20271083)
SHINOHARA Yasuo  Fac.Pharm.Sci., Univ.Tokushima, Associate professor, 薬学部, 助教授 (60226157)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥31,200,000 (Direct Cost: ¥31,200,000)
Fiscal Year 2000: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1999: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1998: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1997: ¥12,600,000 (Direct Cost: ¥12,600,000)
Keywordstype II hexokinase / energy metabolism / oxidative phosphorylation / glycolysis / anti-tumor drugs / porin / type 1 glucose transporter / mitochondria / ヘキソキナーゼ / HIF / 転写阻害剤
Research Abstract

Various chemicals have been established as potential candidates of anticancer drugs. However, due to their low selective toxicity against tumor cells, most of them show strong side effects. To overcome this problem, chemicals showing highly selective toxicity against tumor cells must be established. To explore desirable candidates of anti-tumor drugs, in this study, we tried to characterize the energy metabolism specifically held in tumor cells. As a result, following results were obtained.
1. We found that the transcript level of type II hexokinase was remarkable in malignant tumor cells. Quantitative analysis showed that the level of its expression in tumor cells were as high as 2.5 fmol/mg poly(A)^+ RNA.
2. We next explored under which condition the expression of type II hexokinase become remarkable. As a result, transcript level of type II hexokinase was remarkable when AH130 cells were grown in an abdominal cavity of rats. However, when this cell line was grown in culture dishes, the transcript level of type II hexokinase was remarkably lower than that observed with ascites cells. These results indicate that the transcript level of type II hexokinase could be changes accompanied by changes in culture conditions.
3. To understand how such remarkable change is caused, we examined possible involvement of hypoxia and serum stimulation. As a result, gene expression of type II hexokinase was remarkably elevated by serum stimulation.
Based on these results, we concluded that the metabolic pathway of sugar in tumor cells could be changed accompanied by changes in their growth conditions and that this change is mainly mediated by serum responsive factor. These findings are very helpful for design and development of new anti-tumor drugs. We are now exploring candidate compounds which specifically suppress the growth of tumor cells.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (86 results)

All Other

All Publications (86 results)

  • [Publications] Y.Shinohara et al.: "Source of ATP for hexokinase-catalyzed glucose phosphorylation in tumor cells : Dependence on the rate of oxidative phosphorylation to that of extramitochondrial ATP generation"Biochim.Biophys.Acta. 1319. 319-330 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Yamazaki et.al.: "Structural features of the gene encoding human muscle type carnitine palmitoyltransferase 1"FEBS Lett.. 409. 401-406 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Daikoku et al.: "Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure"FEBS.Lett. 410. 383-386 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Ushimaru et al.: "Non-hydrated states of the acyl phosphate group in the phosphorylated intermediate of(Na^+, K^+)-ATPase."J.Biochem.. 122. 666-674 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Terada et al.: "Important role of loops in the transport activity of the mitochondrial ADP/ATP carrier"Progr.Colloid Polym.Sci.. 106. 192-197 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Goto et al.: "Dynamic structural features of macrocyclic Cytocharasin analogues responsible for their hexose transport inhibition"J.Am.Chem.Soc.. 120. 2457-2463 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara et al.: "Quantitative determinations of the steady state transcript levels of hexokinase isozymes and glucose transporter isoforms in normal rat tissues and the malignant tumor cell line AH130"Biochim.Biophys.Acta. 1368. 129-136 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Majima et al.: "Binding of the fluorescein derivative Eosin Y to the mitochondrial ADP/ATP carrier : Characterization of the adenine nucleotide binding site"Biochemistry. 37. 424-432 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara et al.: "Cationic uncouplers of oxidative phosphorylation are inducers of mitochondrial permeability transition."FEBS.Lett. 428. 89-92 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Abe et al.: "Isolation and characterization of cDNA encoding rat muscle type acetyl-CoA carboxylase"Biochim.Biophys.Acta. 1398. 347-352 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kogure et al.: "Inhibitory effects of pentamethine trinuclear cyanine dyes on ADP/Fe2+-induced lipid peroxidation in rat liver mitochondria : changes in the mode of action with the hydrophobic nature of the dyes."Biol.Pharm.Bull.. 21. 180-183 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ohkura et al.: "Effect of extracellular calcium on the intercellular calcium level of new born rat skin basal cells"Biol.Pham.Bull.. 21. 1-4 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hashimoto et al.: "Fluctuation of the first loop facing the matrix of the mitochondrial ADP/ATP carrier deduced from intermolecular cross- linking of Cys56 residues by bifunctional dimaleimide."Biochemistry. 38. 1050-1056 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hashimoto et al.: "Expression of bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria : Significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carrier."Biochim.Biophys.Acta. 1409. 113-124 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Hatanaka et al.: "Functional expression of the tandem-repeated homodimer of the mitochondrial ADP/ATP carrier in Saccharomyces cerevisiae."Biochim.Biophys.Res.Commun.. 262. 726-730 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kogure et al.: "Potent antiperoxidation activity of the bisbenzylisoquinoline alkaloid cepharanthine : the amine moiety is responsible for its pH-dependent radical scavenge activity."Biochim.Biophys.Acta. 1426. 133-142 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Abe et al.: "Effect of endogenous nitric oxide on energy metabolism of rat heart mitochondria during ischemia and reperfusion"Free Radic.Biol.Med.. 26. 379-387 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hashimoto et al.: "Irreversible Extrusion of First Loop Facing the matrix of the Bovine Heart Mitochondrial ADP/ATP Carrier by Labeling the Cys^<56> Residue with the SH-Reagent Methanethiosulfonate^1."J.Biochem.. 127. 443-449 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Daikoku et al.: "Specific elevation of transcript levels of particular protein subtypes induced in brown adipose tissue by cold exposure"Biochim.Biophys.Acta. 1457. 263-272 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Yamazaki et al.: "Novel Expression of Equivocal Messages Containing Both Regions of Choline/Ethanolamine Kinase and Muscle Type Carnitine Palmitoyltransferase I"The Journal of Biological Chemistry. 275. 31739-31746 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.Aoyama et al.: "Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes α and β^1"Journal of Lipid Research. 141. 452-464 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara et al.: "Characterization of porin isoforms expressed in tumor cells"Eur.J.Biochem.. 267. 6067-6073 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara et al: "Roles of hexokinase in aberrant sugar metabolism of tumor cells"Recent Res.Devel.Bioenerg. 1. 1-8 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara and H.Terada: "Uncouplers of Oxidative Phosphorylation : Activities and Physiological Significance"In Membrane Structure in Disease and Drug Therapy, Marcel Dekker.New York. 1. 107-126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara et al.: "Growth condition dependent synchronized changes in the transcript levels of type II hexokinase and type 1 glucose transporter in tumor cells."Biochim.Biophys.Acta. 1499. 242-248 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Majima et al.: "Specific labeling of the bovine heart mitochondrial phosphate carrier with fluorescein-5-isothiocyanate : roles of Lys 185 and putative adenine nucleotide recognition site in phosphate transport"J.Biol.Chem. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara, et al.: "Source of ATP for hexokinase-catalyzed glucose phosphorylation in tumor cells : Dependence on the rate of oxidative phosphorylation to that of extramitochondrial ATP generation."Biochim.Biophys.Acta. 1319. 319-330 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Yamazaki, et al.: "Structural feature of the gene encoding human muscle type carnitine palmitoyltransferase I."FEBS Lett.. 409. 401-406 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Daikoku, et al.: "Dramatic enhancement of the specific expression of the heart-type fatty acid binding protein in rat brown adipose tissue by cold exposure."FEBS Lett.. 410. 383-386 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Ushimaru, et al.: "Non-hydrated states of the acyl phosphate group in the phosphorylated intermediate of (Na^+, K^+)-ATPase."J.Biochem.. 122. 666-674 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Terada and E.Majima: "Important role of loops in the transport activity of the mitochondrial ADP/ATP carrier.(review)"Progr.Colloid Polym.Sci.. 106. 192-197 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Goto, et al.: "Dynamic structural features of macrocyclic Cytocharasin analogues responsible for their hexose transport inhibition."J.Am.Chem.Soc.. 120. 2457-2463 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara, et al.: "Quantitative determinations of the steady state transcript levels of hexokinase isozymes and glucose transporter isoforms in normal rat tissues and the malignant tumor cell line AH130"Biochim.Biophys.Acta. 1368. 129-136 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Majima, et al.: "Binding of the fluorescein derivative Eosin Y to the mitochondrial ADP/ATP carrier : Characterization of the adenine nucleotide binding site."Biochemistry. 37. 424-432 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara, et al.: "Cationic uncouplers of oxidative phosphorylation are inducers of mitochondrial permeability transition."FEBS Lett.. 428. 89-92 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Abe, et al.: "Isolation and characterization of cDNA encoding rat muscle type acetyl-CoA carboxylase."Biochim.Biophys.Acta. 1398. 347-352 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kogure, et al.: "Inhibitory effects of pentamethine trinuclear cyanine dyes on ADP/Fe^<2+>-induced lipid peroxidation in rat liver mitochondria : changes in the mode of action with the hydrophobic nature of the dyes."Biol.Pharm.Bull.. 21. 180-183 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ohkura, et al.: "Effect of extracellular calcium on the intracellular calcium level of newborn rat skin basal cells."Biol.Pharm.Bull.. 21. 1-4 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hashimoto, et al.: "Fluctuation of the first loop facing the matrix of the mitochondrial ADP/ATP carrier deduced from intermolecular cross-linking of Cys^<56> residues by bifunctional dimaleimide."Biochemistry. 38. 1050-1056 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hashimoto, et al.: "Expression of bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria : Significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carrier."Biochim.Biophys.Acta. 1409. 113-124 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Hatanaka, et al.: "Functional expression of the tandem-repeated homodimer of the mitochondrial ADP/ATP carrier in S.cerevisiae."Biochem.Biophys.Res.Commun.. 262. 726-730 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kogure, et al.: "Potent antiperoxidation activity of the bisbenzylisoquinoline alkaloid cepharanthine : the amine moiety is responsible for its pH-dependent radical scavenge activity."Biochim.Biophys.Acta. 1426. 133-142 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Abe, et al.: "Effect of endogenous nitric oxide on energy metabolism of rat heart mitochondria during ischemia and reperfusion."Free Radic.Biol.Med.. 26. 379-387 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Hashimoto, et al.: "Irreversible extrusion of the first loop facing the matrix of the bovine heart mitochondrial ADP/ATP carrier by labeling the Cys^<56> residue with the SH-reagent methyl methanethiosulfonate."J.Biochem.. 127. 443-449 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Daikoku, et al.: "Specific elevation of transcript levels of particular protein subtypes induced in brown adipose tissue by cold exposure."Biochim.Biophys.Acta. 1457. 263-272 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Yamazaki, et al.: "Novel expression of equivocal messages containing both regions of choline/ethanolamine kinase and muscle type carnitine palmitoyltransferase I"J.Biol.Chem.. 275. 31739-31746 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.Aoyama, et al.: "Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes α and β."J.Lipid Res.. 41. 452-464 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara, et al.: "Characterization of porin isoforms expressed in tumor cells."Eur.J.Biochem.. 267. 6067-6073 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara and H.Terada: "Roles of hexokinase in aberrant sugar metabolism of tumor cells.(review)"Recent Res.Devel.Bioenerg.. 1. 1-8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara and H.Terada: "Uncouplers of oxidative phosphorylation in mitochondria.(review)"Membrane Structure in Disease and Drug Therapy, Edited by G.D.Zimmer, Marcel Dekker, New York. 107-126 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Shinohara, et al.: "Growth condition dependent synchronized changes in the transcript levels of type II hexokinase and type 1 glucose transporter in tumor cells."Biochim.Biophys.Acta. 1499. 242-248 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Majima, et al.: "Specific labeling of the bovine heart mitochondrial phosphate carrier with fluorescein-5-isothiocyanate : Roles of Lys^<185> and putative adenine nucleotide recognition site in phosphate transport."J.Biol.Chem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Daikoku et al.: "Specific elevation of transcript levels of particular protein subtypes induced in brown adipose tissue by cold exposure"Biochim.Biophys.Acta. 1457. 263-272 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Yamazaki et al.: "Novel Expression of Equivocal Messages Containing Both Regions of Choline/Ethanolamine Kinase and Muscle Type Carnitine Palmitoyltransferase I"The Journal of Biological Chemistry. 275-41. 31739-31746 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Shinohara et al.: "Characterization of porin isoforms expressed in tumor cells"Eur.J.Biochem.. 267. 6067-6073 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] C.Aoyama et al.: "Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes α and β^1"Journal of Lipid Research. 141-3. 452-464 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Hashimoto et al.: "Irreversible Extrusion of First Loop Facing the matrix of the Bovine Heart Mitochondrial ADP/ATP Carrier by Labeling the Cys^<56> Residue with the SH-Reagent Methanethiosulfonate^1."J.Biochem.. 127. 443-449 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Shinohara and H.Terada: "Uncouplers of Oxidative Phosphorylation : Activities and Physioiogical Significance"Marcel Dekker.New York. 1. 107-126 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Shinohara et al: "Roles of hexokinase in aberrant sugar metabolism of tumor cells"Recent Res.Devel.Bioenerg. 1. 1-8 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Shinohara et al.: "Growth condition dependent synchronized changes in the transcript levels of type II hexokinase and type 1 glucose transporter in tumor cells."Biochim.Biophys.Acta. 1499. 242-248 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] E.Majima et al.: "Specific labeling of the bovine heart mitochondrial phosphate carrier with fluorescein-5-isothiocyanate : roles of Lys 185 and putative adenine nucleotide recognition site in phosphate transport"J.Biol.Chem.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Hashimoto et al.: "Fluctuation of the first loop facing the matrix of the mitochondrial ADP/ATP carrier deduced from intermolecular cross-linking of Cys56 residues by bifunctional dimaleimide."Biochemistry. 38. 1050-1056 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Hashimoto et al.: "Expression of bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: Significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carrier,by the corresponding regions"Biochim.Biophys.Acta. 1409. 113-124 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Hatanaka et al.: "Functional expression of the tandem-repeated homodimer of the mitochondria ADP/ATP carrier in Saccharomyces cerevisiae."Biochem.Biophys.Res.Commun.. 262. 726-730 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Abe et al.: "Effect of endogenous nitric oxide on energy metabolism of rat heart mitochondria during ischemia and reperfusion."Free Radic.Biol.Med.. 26. 379-387 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Kogure et al.: "Potent antiperoxidation activity of the bisbenzylisoquinoline alkaloid cepharanthine: the amine moiety is responsible for its pH-dependent radical scavenge activity."Biochim.Biophys.Acta. 1426. 133-142 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 寺田 弘: "地球にやさしい化学と環境問題"日本油化学会誌. 48. 401-408 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Hashimoto et al.: "Irreversible extrusion of the first loop facing the matrix of the bovine heart mitochondrial ADP/ATP carrier by labeling the Cys56 residue with the SH-reagent methyl methanethiosulfonate."J.Biochem.印刷中. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Daikoku et al.: "Specific elevation of transcript levels of particular protein subtypes induced in brown adipose tissue by cold exposure"Biochim.Biophys.Acta 印刷中. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Shinohara and H.Terada: "Uncouplers of oxidative phosphorylation in mitochondria"Membrane Structure in Disease and Drug Therapy,Edited by G.D.Zimmer,Marcel Dekker,New York.印刷中. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Shinohara and H.Terada: "Roles of hexokinase in aberrant sugar metabolism of tumor cells"Recent Research Development in Bioenergetics,Edited by S.G.Pandalai,Transworld Research NetWork,Trivandrum.印刷中. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] E.Majima et al.: "Binding of the Fluorescein Derivative Eosin Y to the Mitochondrial ADP/ATP Carrier:Characterization of the Adenine Nucleotide Binding Site" Biochemistry. 37-1. 424-432 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Hashimoto et al.: "Fluctuation of the First Loop Facing the Matrix the Mitochondrial ADP/ATP Carrier Deduced from Intermolecular Cross-Linking of Cys^<56> Residues by Bifunctional Dimateimides" Biochemistry. 38-3. 1050-1056 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Goto et al.: "Dynamic Structural Features of Macrocyclic Cytochalasin Analogues Responsible for Their Hexose Transport Inhibition" J.Am.Chem.Soc.120-10. 2457-2463 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Kogure et al.: "Inhibitory Effects of Pentamethine Trinuclear Cyanine Dyes on ADP/Fe^<2+>-induced Lipid Peroxidation in Rat Liver Mitochondria:Changes in the Mode of Action with the Hydrophobic Nature of the Dyes" Biol.Pharm.Bull.21-2. -183 (1998)

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  • [Publications] Y.Shinohara et al.: "Cationic uncouplers of oxidative phosphorylation are inducers of mitochondrial permeability transition" FEBS Letters. 428. 89-92 (1198)

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  • [Publications] K.Abe et al.: "Isolation and characterization of cDNA encoding rat heart type acetyl-CoA carboxylase" Biochim.Biophys.Acta. 1398. 347-352 (1998)

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  • [Publications] K.Abe et al.: "Effect of Endogenous Nitric Oxide on Energy Metabolism of Rat Heart Mitochondria During Ischemia and Reperfusion" Free Radical Biology and Medicine. 26-3/4. 379-387 (1998)

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  • [Publications] M.Hashimoto et al.: "Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria:significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers" Biochim.Biophys.Acta. 1409. 113-124 (1998)

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  • [Publications] 真島英司、寺田弘: "ATPはいかにして膜を透過するか:ループの協調的スウィングによるミトコンドリアADP/ATPキャリアーの機能発現" 生物物理. 38-6. 245-249 (1998)

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  • [Publications] Y.Shinohara et al.: "Quantitative determinations of the steady state transcript levels of hexokinase isozymes and glucose transporter isoforms in normal rat tissues and the malignant tumor cell line AH130" Biochim.Biophys.Acta. 1368. 129-136 (1998)

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  • [Publications] Y.Shinohara, et al.: "Source of ATP for hexokinase-catalyzed glucose phosphorylation in tumor cells:Dependence on the rate of oxidative phosphorylation to that of extramitochondrial ATP generation" Biochim.Biophys.Acta. 1319. 319-330 (1997)

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  • [Publications] H.Terada, et al.: "Important role of loops in the transport activity of the mitochondrial ADP/ATP carrier" Progr.Colloid Polym.Sci.106. 192-197 (1997)

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  • [Publications] Y.Shinohara, et al.: "Quantitative determinations of the steady state transcript levels of hexokinase isozymes and glucose transporter isoforms in normal rat tissues and the malignant tumor cell line AH130" Biochim.Biophys.Acta.1368. 129-136 (1998)

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  • [Publications] E.Majima, et al.: "Rinding of the fluorescein derivative Eosin Y to the mitochondrial ADP/ATP carrier:Characterization of the adenine nucleotide binding site" Biochemistry. 37. 424-432 (1998)

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  • [Publications] 篠原康雄、寺田 弘: "がん細胞のエネルギー代謝系のキャラクタリゼーション" サイエンスリポート. (印刷中).

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Published: 1997-04-01   Modified: 2016-04-21  

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