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Effect of Food Components on Growth of Escherichia coli O157

Research Project

Project/Area Number 09358001
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 家政学
Research InstitutionNara Women's University

Principal Investigator

YOKOIGAWA Kumio  Nara Women's University, Fuculty of Human Life and Environments, Associate Professor, 生活環境学部, 助教授 (60230637)

Co-Investigator(Kenkyū-buntansha) 河合 弘康  奈良女子大学, 生活環境学部, 教授 (80026525)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥19,200,000 (Direct Cost: ¥19,200,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1997: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsEscherichia coli O157 / Food Hygiene / Acid tolerance / 香辛料 / 増殖抑制 / 大腸菌O-157
Research Abstract

Escherichia coli O157 was first recognized as a cause of illness in 1982. Bovine products have most often been implicated in food-borne infections with E.coli O157. However recent outbreaks indicate that other food types may also serve as vehicles of transmission for this pathogen. E.coli O157 strains are reported to be tolerant to low pH and low water activity. In particular, the acid tolerance is important property of E.coli O157 because E.coli O157 must pass through the acidic gastric barrier to cause gastrointestinal disease. Acid resistance of E.coli O157 cells depends on their growth conditions. To clarify the reason for the infection of this pathogen, the survival and growth of E.coli O157 in foods were investigated. The productin of verotoxin by E.coli O157 in foods was also investigated.
When E.coli O157 : H7 was incubated with the various seasonings, the O157 strains showed a high tolerance to soy sauce. E.coli O157 strains incubated with 12.5% soy sauce grew well, but the viable non-pathogenic E.coli populations were reduced. When the O157 strains were inoculated on LB agar media containing 14% soy sauce, all three E.coli O157 : H7 strains formed visible colonies, but all three non-pathogenic E.coli strains could not form visible colonies.
When the E.coli cells were incubated with ethanol-soluble fractions of various spices, the E.coli O157 strains showed lower survival ratios in an ethanol-soluble fraction of nutmeg. The population of non-pathogenic E.coli was not reduced under the same conditions. The nutmeg extract may be useful for specific sterilization of E.coli O157.
The acid resistance of E.coli O157 grown in vegetables was found to be low in comparison with that of the cells grown in EC medium.
The production of verotoxin in E.coli O157 grown in EC medium was clearly higher than that of verotoxin in E.coli O157 grown in vegetable extracts. This suggest that some components of vegetables might prevent E.coli O157 from producing verotoxin.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Kumio Yokoigawa: "Evaluation of an Alanine Racemase Gene as an Indicator for the Detection of Various Escherichia coli : Reactivity of the Gene Fragment with Various E.coli O157: H7 Isolates."J.Ferment.Bioeng.. 84. 471-474 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoko Okubo: "Alanine Racemase Gene Fragments as Probes for Detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in Foods."J.Ferment.Bioeng.. 85. 559-563 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoko Okubo: "Effect of Ethyl Alcohol on Growth and Intracellular Alanine Racemase of Psychrotrophs."J.Biosci.Bioeng.. 87. 241-244 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoko Okubo: "Characterization of Psychrophilic Alanine Racemase from Bacillus psychrosaccharolyticus"Biochem.Biophys.Res.Commun.. 256. 333-340 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumio Yokoigawa: "Primers for Amplifying an Alanine Racemase Gene Fragment to Detect E.coli strains in Foods."J.Food Sci.. 64. 571-575 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumio Yokoigawa: "Difference between Escherichia coli O157 :H7 and Non-pathogenic E.coli : Survival and \Growth in Seasonings."J.Biosci.Bioeng.. 88. 574-576 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumio Yokoigawa: "Evaluation of an Alanine Racemase Gene as an Indicator for the Detection of Various Escherichia coli : Reactivity of the Gene Fragment with Various E.coli O157 : H7 Isolates."J.Ferment.Bioeng.. 84. 471-474 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoko Okubo: "Alanine Racemase Gene Fragments as Probes for Detecting Bacillus stearothermophilus and Bacillus psychrosaccharo-lyticus in Foods."J.Ferment.Bioeng.. 85. 559-563 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoko Okubo: "Effect of Ethyl Alcohol on Growth and Intracellular Alanine Racemase of Psychrotrophs."J.Biosci.Bioeng.. 87. 241-244 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoko Okubo: "Characterization of Psychrophilic Alanine Racemase from Bacillus psychrosaccharolyticus"Biochem.Biophys.Res.Commun.. 256. 333-340 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumio Yokoigawa: "Primers for Amplifying an Alanine Racemase Gene Fragment to Detect E.coli strains in Foods."J.Food Sci.. 64. 571-575 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumio Yokoigawa: "A New Sterility Test for Cow's Milk Using Alanine Racemase Gene as the Index."J.Biosci.Bioeng.. 88. 26-29 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumio Yokoigawa: "Difference between Escherichia coli O157 : H7 and Non-pathogenic E.coli : Survival and Growth in Seasonings."J.Biosci.Bioeng.. 88. 574-576 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoko Okubo: "High Catalytic Activity of Alanine Racemase from Psychrophilic Bacillus psychro-saccharolyticus at High Temperatures in the Presence of Pyridoxal 5'-phosphate."FEMS Microbiol.Lett,. 192. 169-173 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Reiko Hirasawa: "Leavening Ability of Bakers' Yeast Exposed to Hyperosmotic Media."FEMS Microbiol.Lett. 194. 159-162 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Reiko Hirasawa: "Loading of Bakers' Yeast with Trehalose for Improvement of Its Freeze-tolerance."Biosci.Biotechnol.Biochem.. 65. 522-526 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kumio Yokoigawa: "Structure and function of Psychrophilic Alanine Recemase."J.Mol.Cat.B:Enzymatic. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Kumio Yokoigawa: "Difference between Escherichia coli O157:H7 and Non-pathogenic E.coli: Survival and Growth in Seasonings"J.Biosci.Bioeng.. 88. 574-576 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kumio Yokoigawa: "Primers for Amplifying an Alanine Racemase Gene Fragment to Detect E.coli strains in Foods"J.Food Sci.. 64. 571-575 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kumio Yokoigawa: "A New Sterility Test for Cow's Milk Using Alanine Racemase Gene as the Index"J.Biosci.Bioeng.. 88. 26-29 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kumio Yokoigawa: "An alanine racemase gene as a new index for detecting Escherichia coli in foods" Biosci.Biotech.Biochem..60. 1799-1804 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kumio Yokoigawa: "Evaluation of an alanine racemase gene as an indicator for the detection of various Escherichia coli; reactivity of the gene fragment with various E.coli O157:H7 isolates." J.Ferment.Bioeng.84. 471-474 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoko Okubo: "Evaluation of alanine racemase gene as a new indicator for detecting bacteria in foods" Recent Res.Devel.Ferment.Bioeng.2. 1-19 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoko Okubo: "Effect of ethyl alcohol on growth and intracellular alanine racemase of psychrotrophs." J.Biosci.Bioeng.87. 241-244 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kumio Yokoigawa: "An alanine racemase gene as a new index for detecting Escherichia coli in foods" Biosci.Biotech.Biochem.60. 1799-1804 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kumio Yokoigawa: "Evaluation of an alanine racemase gene as an indicator for the detection of various Escherichia coli ; reactivity of the gene fragment with various E.coli O157 : H7 isolates." J.Ferment.Bioeng.84. 471-474 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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