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Hormonal control of pupal commitment and programmed cell death

Research Project

Project/Area Number 09440273
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionKanazawa University

Principal Investigator

SAKURAI Sho  Department of biology, Kanazawa University, Professor, 理学部, 教授 (80143874)

Co-Investigator(Kenkyū-buntansha) IWAMI Masafumi  Department of biology, Kanazawa University, Associate P., 理学部, 助教授 (40193768)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥9,000,000 (Direct Cost: ¥9,000,000)
Keywordsecdysone / juvenile hormone / determination / apoptosis / anterior silk gland / forewing disc / 翅成虫原基 / カイコ / 20-ヒドロキシエクジソン / カイコガ / 蛹コミットメント / 絹糸腺 / エクジステロイド
Research Abstract

Present research determined and showed the followings during the propqsed period.
(1) Hormone titer : Hemolymph juvenile hormone and ecdysteroid titers were determined every 12 h for JH during a period from third stadium to pupation and every 2 h for ecdysteroids from 4th stadium through pupation.
(2) Pupal commitment of for wing discs : The change in commitment in wing discs was found to be finished for 16 h after last larval ecdysis. 20-Hydroxyecdyosne did not accelerate the rate of the change and JH failed to suppress the change at a physiological dose. JH also did not affect the change in commitment by. 20E. The change in commitment may be initiated at the time of head capsule slippage (HCS). After HCS, the discs became responsive dramatically to 20E while lost their responsiveness to JH. The discs of early 4th stadium, that were not pupally committed by 20E in vitro, were able to be pupally committed by two step incubation, first with no hormone followed by 20E challenge. In such conditions, the discs were pupally committed and the commitment was strongly suppressed by JH at a concentration lower than physiological one.
(3) Programmed cell death of anterior silk gland : Apoptosis of anterior silk gland is induced by 20E in vitro. After 20E challenge, gene expression and protein synthesis necessary for the death were completed within 8 and 18 h, respectively. Nevertheless, 20E must be present for 42 h for completion of the death. Along with other evidence, 20E was suggested to act after 18 h through a membrane-bound receptor and the second messenger is cyclic AMP. Seven early genes have been cloned and five of them exhibited homology of known genes in other animals but two did not possess open reading frame. Analysis of their function is under progress.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Niimi S, Sakurai S: "Developmental changes in juvenile hormone and…"J. Insect Physiol.. 43. 875-884 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oda Y, Iwami M 他: "Dynamics of haemolymph sorbitol-6-phosphate…"Insect Biochem. Mol. Biol.. 27. 461-468 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakurai S, Kayama M, 他: "Hemolymph ecdysteroid titer and ecdysteroid…"J. Insect Physiol.. 44. 867-881 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oda Y, Umerima M, 他: "Role of ecdysteroids in the dynamics of insect…"Zool. Sci. 17. 785-789 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terashima T.他: "Programmed Cell death triggered by insect…"Dev. Gens Evol.. 210. 545-558 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuzuki, S. 他: "Ecdysteroid-inducible genes in the programmed…"Insect biochem. Mol. Biol.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Niimi S. and Sakurai S.: "Developmental changes in juvenile hormone and juvenile hormone acid titers in the hemolymph and in vitro juvenile hormone synthesis by corpora allata of the silkworm, Bombyx mori"J.Insect Physiol.. 43. 875-884 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida I., Tsuzuki S., Salam S.E.A., Ino M., Korayem A.M., Sakurai S. and Iwami M.: "Bombyxin F1 gene : Structure and expression of a new bombyxin family gene that forms a pair with bombyxin B10 gene"Zool Sci.. 14. 615-622 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oda Y., Iwami M., Osanai M. and Sakurai S.: "Dynamics of haemolymph sorbitol-6-phosphate and its control by ecdysteroid in the larvae of the silkworm, Bombyx mori"Insect Biochem.Mol.Biol.. 27. 461-468 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuzuki S., Masuta T., Furuno M., Sakurai S. and Iwami M.: "Structure and expression of bombyxin E1 gene : A novel family gene that encodes bombyxin-IV, and insect insulin-related neurosecretory peptide"Comp.Biochem.Physiol.. 117B. 409-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida I., Moto K., Sakurai S. and Iwami M.: "A novel member of the bombyxin gene family : structure and expression of Bombyxin G1, an insulin-related peptide gene of the silkmoth bombyx mori"Dev.Genes Evol.. 208. 407-410 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakurai S., Kaya M. and Satake S.: "Hemolymph ecdysteroid titer and exdysteroid-dependent developmental events in the last-larval stadium of the silkworm, Bombyx mori : role of low ecdysteroid titer in larval-pupal metamorphosis and a reappraisal of the head critical period"J.Insect Physiol.. 44. 867-881 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Moto, K., Salam, S., Sakurai, S. and Iwami, M.: "Gene transfer into insect brain and cell-specific expression of bombyxin gene"Dev.Genes Evol.. 209. 447-450 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Singtripop, T., Wanichacheewa, S., Tsuzuki, S., and Sakurai, S.: "Larval growth and diapause in a tropical moth, Omphisa fuscidentalis Hampson"Zool.Sci.. 16. 725-733 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Singtripop, T., Wanichacheewa, S. and Sakurai, S.: "Juvinile hormone-mediated termination of larval diapause in the bamboo borer, Omphisa fuscidentalis"Insect Biochem.Mol.Biol.. 30. 847-854 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oda, Y., Uejima, M., Iwami, M. and Sakurai, S.: "Role of ecdysteroids in the dynamic of insect haemolymph sugar"Zool.Sci.. 17. 785-789 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terashima, T., Yasuhara, N., Iwami, M. and Sakurai, S.: "Programmed cell death triggered by insect steroid hormone, 20-hydroxyecdysone, in the anterior silk gland of the silkworm, Bombyx mori"Dev.Genes Evol.. 210. 545-558 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuzuki, S., Iwami M. and Sakurai, S.: "Ecdysteroid-inducible genes in the programmed cell death during insect metamorphosis"Insect Biochem.Mol.Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Moto et al.: "Gene transfer into insect brain and cell-specific expression of Bombysa gene"Dev. Genes Evol.. 209. 447-450 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Singtripop et al.: "Larval growth and diapause in a tropical moth, Omphisa fuscidentalis Hampson"Zool. Sci.. 16. 725-733 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Oda et al.: "Role of ecdysteroids in the dynamics of insect hemolymph sugar"Zool. Sci.. 17(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sakusai,S.他: "Flewolegurph ecdysteroid titer and ecdysteroid-dependent developmental eveuts in the last larval stadium of the Silkworm,Bombyx mori." J.Insect Physiol.44巻. 867-881 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida,I他: "A novel member of the bombyxinu gene family:structure and expression of bombyxiu G1,an insuliu-related peptide gene of the silkmoth,Bombyx mori" Dev.Genes Evol.208巻. 407-410 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Moto,K.他: "Gene transfer into insect brain and cell-specific expression of bombyxin gene" Dev.Genes Evol.印刷中. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Niimi,S & Sakura,S: "Developmental Changes in Juvenile hormone and ...." Journal of Insect Physiology. 43. 875-884 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Oda,Y.et al.: "Dynamics of haemolymph sorbito-6-phosphate and ...." Insect Biochemistry and molecular biology. 27. 461-468 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida,I.et al.: "Bombyxin F1 gene; Structure and expression of a ...." Zoological Science. 14. 615-622 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsuzuki,S.et al.: "Structure and expression of bombxin E1 gene: A novel...." Comparative Biochemistry and Physiology. 117B. 409-416 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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