| Project/Area Number |
09450303
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Graduate School of Engineering, Nagoya University |
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Principal Investigator |
IIJIMA Shinji Prof.Dept.of Biotechnol.Grad.School of Eng., Nagoya Univ., 大学院・工学研究科, 教授 (00168056)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIJIMA K. Assis.Prof.Dept.of Biotechnol.Grad.School of Eng., Nagoya Univ., 工学研究科, 助手 (10262891)
MIYAKE K. Assis.Prof.Dept.of Biotechnol.Grad.School of Eng., Nagoya Univ., 工学研究科, 助手 (90252254)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 1998: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1997: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | Streptococcus agalactiae / Cell adheasion / Sialyl Lewis Sugar / Polysialic acid / Sialydase / シアリダーゼ / B群連鎖球菌 / エンド-β-ガラクトシダーゼ / リンパ球 / ナチュラルキラー細胞 / 免疫 |
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| Research Abstract |
We are studying biological functions of sugar and its relation to disease. Polysialic acid is suggested to relate cancer-metastasis in addition to the function as a component of N-CAM.We therefore purified sialydase from a coliphage and cloned the enzyme gene. This sialydase was unique as endosialydase, since minimun substrate size was sialyltrimer and the final product from polysialic acid was sialyldimer. The enzyme was homotetramer and composed of 120 Kd subunit.
We also studied the capsular polysaccharide of Streptococcus agalactiae which has sialyl lactosamine polymer. Sialyl lactosamine is an analog of sialyl Lewis sugar which binds L-selection. We therefore studied the function of this sugar by using anti-L-selection antibody. We found that the antibody induced the aggregation of T-lymphocytes by elevating intracellular calcium concentration. We also found that T-cell growth by the stimulation of staphyrococcul superantigen was enhanced by anti-selectin antibody.
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