• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on Development of Efficient Transgenic System for Alien Gene of Insecticidal Protein in Sugarbeet

Research Project

Project/Area Number 09460001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SHIMAMOTO Yoshiya  Hokkaido Univ., Grad. School of Agri., Pro., 大学院・農学研究科, 教授 (00001438)

Co-Investigator(Kenkyū-buntansha) KANAZAWA Akira  Hokkaido Univ., Grad. School of Agri., Inst., 大学院・農学研究科, 助手 (30281794)
ASANO Shinichiro  Hokkaido Univ., Grad. School of Agri., Asso. Pro., 大学院・農学研究科, 助教授 (60222585)
SANO Yoshio  Hokkaido Univ., Grad. School of Agri., Pro., 大学院・農学研究科, 教授 (70109528)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1997: ¥7,400,000 (Direct Cost: ¥7,400,000)
Keywordssugarbeet / cabbage armyworm / cry gene / ICP / Agrobacterium / bioassay / bombardment / chloroplast / 殺虫性 / BT遺伝子 / 葉緑体 / 転移酵素 / アグロバクテリウム / ヨトウ
Research Abstract

1)Development of chloroplast transgenic system in sugarbeet
We have progressed to develop a chloroplast transgenic system in sugarbeet in which is introduced cry genes to chloroplast genome and produce efficiently insecticidal crystal protein (ICP) in chloroplast. Plasmid carrying selective factor (aadA), marker factor (GUS), promoter (rrn) and terminator (rps 16) was incorporated into disc tissue of shoot of sugarbeet by microprojectile bombardment. In the tissue culture regeneration plant has not achieved. Repeatedly plasmid was constructed and incorporated into the sugarbeet.
2)Development of concentration system of protein upon organelle in sugarbeet
We have progressed to develop a concentration system of toxic protein upon organelle in sugarbeet. Base sequence coding transit peptide to chloroplast was cloned from tobacco plant and was linked with position of N terminal in GUS gene. Thus the plasmid was constructed and introduced into sugarbeet by Agrobacterium method.
3)Expression of cry gene in transformant sugarbeet
Several transformants carrying cryIA(b) were detected by PCR southern, ICP analysis and PCR southern blotting in half of plants regenerating after procedure of Agrobacterium method. Several leaves of these transformants were supplied for cabbage armyworm with the third larva age. Larva of cabbage armyworm was able to grow normally on leaves with non-cry gene and not to grow on several leaves of transformant.
4)Screening for efficient cry gene to produce insecticidal protein
Screening for efficient cry gene to produce insecticidal protein was conducted for cabbage armyworm. CryIC gene has more insecticidal ability than cryIA(b) in larva of cabbage armyworm.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (1 results)

All Other

All Publications (1 results)

  • [Publications] 堂前 友子: "形質転換(Beta Maritima)における導入遺伝子の器官特異的発現およびコピー数と発現量との関係"てん菜研究会報. 40. 14-20 (1998)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi