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CELLULAR AND MOLECULAR APPROACHES TO THE BREEDING BARRIER IN PLANTS, APOMIXIS.

Research Project

Project/Area Number 09460004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionOsaka Prefecture University (1999)
University of Miyazaki (1997-1998)

Principal Investigator

ADACHI Taiji  Osaka Prefecture University, College of Agriculture, Prof., 農学部, 教授 (50040861)

Co-Investigator(Kenkyū-buntansha) CHEN Langzhang  Miyazaki University, Gene Research Center, Assist. Prof., 遺伝子実験施設, 助手 (40284822)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsApomixis / Guineagrass / Bahiagrass / RT-PCR / Simplified DD Method / Adventitious embryony / バヒア・グラス / RT・PCR法
Research Abstract

Cytological Approach: At first, the evaluation of the apomictic ratio was performed in facultative apomict species, Guineagrass and Bahiagrass by means of Nomarski's differential interference contrast mirography (DIC). Then, virietal differences of the capability to form adventitious embyos in tissue culture were compared with that of apomictic ratios. There was some corelations among them. In situ hybridization experiment was also carried out at mRNA levels in each developmental stages of zygotic embryo by using DNA probes concerning to the adventitious embryo formation in alfalfa and rice. To now, unfortunately, technical problems still remain to be resolved.
Molecular Approach: By checking the stage at which apospory is determined in Guineagrass, some candidate of cDNA were isolated from developmentally selected samples by means of simplified Differential Display Methods (DD). The gene expression of the candidates were confirmed by Northern hybridization. And then, homology search of candidate, A2-134 was performed with the BLAST-Version 2.1 PIR database. Database search for sequence homology unvailed a relation to the RD22 gen of Arabidopsis thaliana with 50.9% similarity in nucleotide sequence. The RD22 protein regulates the expression of seed-specific genes and probably also genes in responcse to dehydration stress.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Adacbu et al.: "Embryogonic callus formation from protoplasts dorived from susbension cells of opomictie guineagrass"Breeding Science. 45. 445-448 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen & Adachi: "plant regeneration via somatie ombryogenesis after somatic by brind is aticos between Lycepersicon esc and L Pe*****"SABRAO Jour. 30(1). 25-34 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chem & Adachi: "Igolation and charactarization of a gene expressed dusing embryo sac davelopment in apomictie gninoag***"Jour, Plant Physiol.. 154. 55-62 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chem & Adachi: "Cloning and chanactorization of a DNA expressed ot spospprons embryo initial appearance stage"Aomixis Newsletter. 11. 32-33 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 陳 蘭庄 ら: "胚のう分析に基づく条件的アポシクシスギニアグラスの生殖細胞単離の試み"育種学研究. 2巻. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 穴見栄子 ら: "バヒアグラスにおける多胚種子の発育過程に関する細胞学的ならびにRAPD解析"育種学研究. 1巻. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen, L-Z., L-M.Guan, S.Uozu and T.Adachi: "Expression of ASG (Apomixis-specific gene) in ovary of guineagrass (Panicum maximum) using in situ hybridizaion."Breeding Research. 1. 271 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Anami, E., L-Z.Chen, abd T.Adachi: "Cytological and RAPD analyses on the process of polyembryonic seed development in bahiagrass (Paspalum notatum Flugge)."Breeding Research. 1. 222 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen, L-Z., C.Miyazaki, A.Kojima, A.Saito and T.Adachi: "Isolation and characterizaiton of a gene expressed during early embryo sac development in apomictic guinea grass (Panicum maximum)."Jour.Plant Physiol.. 154. 55-62 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen, L-Z., and T.Adachi: "Cloning and characterization of cDNA expressed at aposporous embryo sac initial cell appearance stage in Guineagrass (Panicum maximum Jasq.)"Apomixis Newsletter. 10. 32-34 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matuda Y., F.Hoffmann and T.Adachi: "Embryogenic callus formation from protoplasts of chinese chive (Allium tuberosum Rottl.)"SABRAO Journal. 30. 91-96 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akashi R., S.Lachmann, F.Hoffman and T.Adachi: "Embryogenic callus formation from protoplasts derived from suspension cells of apomictic guineagrass (Panicum maximum Jacq.)"Breeding Science. 1997. 445-448 (45)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Adachi et. al.: "Embryogenic callus formation from protoplasts derived from suspension cells of apomictic quineagrass"Breeding Science. 45. 445-448 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chen and Adachi et al.: "Plant regeneration via somatic embryogenesis after somatic hybridization between Lycopersicon esc. and L. peruvianus"SABRAO Jour.. 30(1). 25-34 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chen and Adachi et. al: "Isolation and characterization of a gene expressed during early embryo sacderelopment in apo*ictic guiniagrass"Jour. Plant Physiol.. 154. 55-62 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 陳蘭庄 ら: "胚のう分析に基づく条件的アポミクシスギーアグラスの生殖細胞単離方式"育種学研究. 2巻(別1). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chen and Adachi et al.: "Cloning and Characterization of a cDNA expressed at aposporons embryo sac initial cell appeaarance stage"Apomixis Newsletter. 11. 32-33 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chen,Miyazaki,Kojima,Saito,Adachi: "Isolation and characterization of a gene expressed during early embryo sac development in apomictic guinegra" j.Plant Physiology. 154. 55-62 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Adachi et al: "Embyogenic callus formation from protoplasts derived from suspension cells of apomictic guineagrass" Breeding Science. 45. 445-448 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Chen and Adachi: "Isolation and characterization of a gene expressed during early embryosac development in apomictic guinea" Plant Science. (submit).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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