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The study of a mechanism for occurrence of bud sport and a method of identification of bud sport strains in apple

Research Project

Project/Area Number 09460013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionHIROSAKI UNIVERSITY

Principal Investigator

ARAKAWA Osamu  Hirosaki University, Faculty of Agriculture and Life Science, Professor, 農学生命科学部, 教授 (70184265)

Co-Investigator(Kenkyū-buntansha) HARADA Takeo  Hirosaki University, Faculty of Agriculture and Life Science, Associate Professor, 農学生命科学部, 助教授 (10228645)
ASADA Takenori  Hirosaki University, Faculty of Agriculture and Life Science, Associate Professor, 農学生命科学部, 助教授 (10003518)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsAFLP / apple / anthocyanin / bud sport / retroposon / フラボノイド / ACC合成酵素
Research Abstract

The purpose of this research is to clarify the mechanism of the occurrence of bud sport in apple and to develop a effective method for selecting a superior strains. The main conclusions drawn from the results are as follows.
1.The effects of light and temperature on anthocyanin accumulation in 'Tsugaru' red sport strains were compared to true -to-type 'Tsugaru' apple. The responsiveness of red sport strains to -UV Iight was larger than that of 'Tsugaru'. There were no clear difference between 'Tsugaru' and red strains for anthocyanin accumulation in response to temperature. The higher phenylalanine-ammonlalyase (PAL) activity was found in red sports strains with light treatment. The composition of anthocyanin and flavonoids of red sport strains were determined and compared to 'Tsugaru'. There were no clear differences in composition of anthocyanin and flavonoids, but the amount of these substances were higher in red sport strains than in 'Tsugaru', which show a higher metabolic activity … More in flavonoid metabolism.
2. Amplified Fragment Length Polymorphism (AFLP) was used to identify apple cultivars. The technique amplified 39 to 80 restriction fragments per primer combination and yielded polymorphic bands that can distinguish from those of closely related seedling cultivars. The technique was then tested to establish whether or not AFLP patterns differ between a cultivar and its sport. Sixty-one primer pairs produced about 2850 bands which were completely identical among two 'Fuji' and three sports trees. However, one of these bands was absent from one of sports tree. These results indicate that AFLP may produce a useful DNA marker for assisting in commercial protection of plant breeders patents. Two transposable elements found in apple genome were characterized. Md-SINE1 was found in the 5' flanking region of Md-ACS1 gene. Its structural feature showed that this element was a member of retroposon group. Majin discovered in the RAPD marker linked a fruit skin color gene was highly reiterated and its number was estimated to be around 6000 per haploid genome. These elements were applied to transposon-based DNA fingerprinting. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] ARAKAWA O.: "Comparison of anthocyanin synthesis of true-to type 'Tsugaru'apple and its red sport strains"J.Hort.Biotech. 74(6). 738-742 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hongqing Wang: "nfluence of maturity and bagging on the relationship between anthocyanin production and phenylalanine ammonia-lyase(PAL) activity in 'Jonathan'apple fruit"Postharvest Bio. & Tech. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 若佐雅也: "OOO.DNA鑑定によるリンゴ'弘大1号'の親品種の同定"弘大農学生命科学部学術報告. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arakawa, O., M. Hiraga, H. Wang, and M. Shinoda: "Comparison of anthocyanin synthesis in true-to-type 'Tsugaru' apple and its red sport strains."The Journal of Horticultural Science & Biotechnology. 74(6). 738-742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hongqing Wang, Osamu Arakawa and Yoshie Motomura: "Influence of maturity and bagging on the relatioship between anthocyanin production and phenylalanine anunonia-lyase (PAL) activity in 'Jonathan ' apple fruit. Postharvest Biology and technology"Postharvest Biology and Technology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] "Identification of the parentage of apple cultivar 'Hirodai lchigou' by DNA-based technique"Bull. Pac. Agric. & Life Sci. Hirosaki Univ.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] ARAKAWA, O.: "Comparison of anthocyanin synthesis of true-to type 'Tsugaru' apple and its red sport strains"J. Hort. Biotech. 74(6). 738-742 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 若佐雄也: "ンゴ果皮赤色化遺伝子Rfに連鎖するDNAマーカーに見いだされたトランスポゾンMd-Tn1について"育種学研究. 1(別冊). 322 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sunako T.et al: "An allele of the ripening-specific-aminocyclopropane-1-carboxylicacid synthase gene(ACS1)in apple fruit with a long storage life" Plant Physiol. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Harada,T.et al: "Md-ACS1,a fruit ripening 1-aminocyclopropane-1-carboxylatesynthase gene of apple" Breeding and Biotechnology for Fruit Trees. NIFTS/CSIRC. 42-45 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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