• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Linkage mapping of peach genome by AFLP analysis.

Research Project

Project/Area Number 09460019
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionKobe University

Principal Investigator

NOMURA Keiichi (1998)  Kobe University, Faculty of Agriculture Associate Professor, 農学部, 助教授 (60252798)

吉田 雅夫 (1997)  神戸大学, 農学部, 教授 (60240313)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Masami  National institute of Fruit Tree Science Department of Breeding Chief, 果樹試験場・育種部, 室長
山口 正巳  農林水産省, 果樹試験場, 室長
野村 啓一  神戸大学, 農学部, 助教授 (60252798)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordspeach / AFLP / polymorphism / RAPD
Research Abstract

The aim of this research was construction of the linkage map of peach genome by AFLP (Amplified Fragment Length Polymorphism) analysis.
Prior to mapping reproducibility and efficiency of AFLP analysis using non-RI labeling (FITC (fluorescein isothiocyanate) or biotin) were confirmed. In addition, AFLP analysis produced 2.5 polymorphic hand per primer between 'Akame' and 'Juseitou'. This corresponded to 20 times more than polymorphism obtained by RAPD (Random Amplified Polymorphic DNA) analysis. Thus, it was concluded that AFLP analysis could provide useful genetic markers to construct the linkage map.
Next, the possibility of AFLP analysis using a combination of AFLP analysis kit obtained from Life Technologies^<TM>, Inc. and Eco primer obtained from ALOKA Co., Ltd. The results suggested that the sequence of Eco RI adapter-primers supplied by Life Technologies^<TM>, Inc. were modified form the original report because no amplified fragments was observed at the step of selective PCR.Therefore, we decided to purchase the Eco primer from Perkin-Elmar Japan Co. Ltd. and to use the model 310 DNA sequencer.
Finally, AFLP analysis was carried out on 'Akatsuki', 'Ogonto' and 45 their F.hybrids. Fifteen out of 51 primers sets exhibited polymorphisms between the parents cultivars. Using the 15 primers sets, the specific fragments, which are characteristic for flesh color and cling- or free-stone, were screened by bulk-analysis. It was found that only one primers set E/ACA and M/CAC, showed polymorphism corresponded to flesh color, yellow or white.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Shimada,T.et al.: "Application of AFLP to Molecular Genetic Analysis in Peach." J.Japan.Soc.Hort.Sci.68(1). 67-69 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada, Y.et al.: "Application of AFLP to Molecular Genetic Analysis in Peach" J.Japan.Soc.Hort.Sci.68 (1). 67-69 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada,T.et al.: "Application of AFLP to Molecular Genetic Analysis in Peach" J.Japan.Soc.Hort.Sci.68(1). 67-19 (1999)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi