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Transmission mechanism of plant viruses transmitted by the fungus Polymyxa betae

Research Project

Project/Area Number 09460028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionOkayama University

Principal Investigator

TAMADA Tetsuo  Research Institute for Bioresources, Professor, 資源生物科学研究所, 教授 (10281156)

Co-Investigator(Kenkyū-buntansha) KONDO Hideki  Research Institute for Bioresouces, Assistant Professor, 資源生物科学研究所, 助手 (40263628)
MAEDA Takanori  Research Institute for Bioresouces, Associate Professor, 資源生物科学研究所, 助教授 (10038309)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsPlant viruses / Benyvirus / BNYVV / Polymyxa / Virus transmission / Molecular biology / Polymyxa菌 / ウイルスの伝搬 / BNTVV
Research Abstract

Beet necrotic yellow vein virus (BNYVV), which is transmitted by the soil-borne fungus Polymyxa betae, contains five ssRNA species (RNAs 1 to 5). BNYVV was transmitted by the biotype of P. batae from sugar beets, but not by the other biotypes from Chenopodium album, Amaranthus retroflexus and Portulaca oleracea. Although host ranges of BNYVV and the vector were thought to be limited to only few species such as Chenopdiaceae species, P. betae zoospores were found to infect a wide range of plant species, and also BNYVV infected roots of several plant species of them. Nicotiana benthamiana is a good indicator host. The coat protein is encoded by the 5' proximal ORF of RNA2, and the terminal codon of this ORF can be suppressed, which results in the production of a 75 kDa readthrough (RT) protein. Mutagenic analysis revealed that a region within the N-terminal half of the RT domain is involved in virus assembly, whereas the C-terminal protein contains sequences important for fungus transmission. Infectious cDNA clones to BNYVV RNAs 3,4 and 5 were established. Fungal inoculation tests with isolates containing different combinations of RNAs 3, 4 and 5 showed that RNA3 and RNA5 are involved in symptom expression. The 25 kDA protein encoded by RNA3 was responsible for rhizomania of susceptible cultivars of sugar beet, and also was involved in resistant reaction in resistant cultivars. When RNA5, encoding the 26 kDA protein, was present, the root symptoms were more intense, Antisera against 25 kDA AND 26 kDA protein, were obtained. It was suggested that expressions of these proteins in infected plant are strongly involved in symptom development. RNA4 was not associated with symptom expression, but acted as increasing transmission efficiency and stability. Synergistic effects were found between RNA4 and RNA3 or RNA5.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] 玉田哲男: "菌類が媒介する植物ウイルス遺伝子の多様性:分子機構とその応用"日本植物病理学会平成9年度植物感染生理談話会. 36-44 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Vascular movement of beet necrotic yellow vein viruses in Beta macrocarpa is probably dependent on an RNA 3 sequence domain rather than a gene product"Journal of General Virology. 79. 385-393 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Molecular and biological aspects of fungal transmission of viruses with rod-shaped particles"Invited Papers Abstracts-Vol. 1, 7th International Congress of Plant Pathology. 1, 13, 7S (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyanishi, M., Kusume, T., Saito, M., and Tamada, T.: "Beet necrotic yellow vein virus RNA5 : geographical distribution and sequence variation"Abstracts-Vol. 2, 7th International Congress of Plant Pathology. 1, 11, 80 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saito, M., Kiguchi, T. and Tamada, T.: "Nonradioactive, digoxigenin-labeled DNA probes for the detection of five RNA species present in beet necrotic yellow vein virus"Bull. Rest. Inst. Bioresour. Okayama Univ.. 5. 79-96 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyanishi, M., Kusume, T., Saito, M., and Tamada, T.: "Evidence for three groups of sequence variants of beet necrotic yellow vein virus RNA 5"Archives of Virology. 144. 879-892 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T., Uchino, H., Kusume, T., Saito, M.: "RNA3 deletion mutants of beet necrotic yellow vein virus do not cause rhizomania disease in sugar beets"Phytopathology. 89. 1000-1006 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T., Miyanishi, M., and Takemoto, M.: "Involvement of beet necrotic yellow vein virus RNA 3in restricted virus multiplication in resistant lines of Beta vulgaris ssp. maritima"Proc. Symp. 4th Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyanishi, M., Kusume, T., Saito, M., and Tamada, T.: "Sequence variation of beet necrotic yellow vein virus RNA 5 : the evolution and the possible route of its spread"Proc. Symp. 4th Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirano, S., Kondo, H., Maeda, T., and Tamada, T.: "Burdock mottle virus has a high genome similarity to beet necrotic yellow vein virus"Proc. Symp. 4th Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 玉田哲男: "ビートネクロティックイエローベインウイルス ウイルス学"朝倉書店. 435-442 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 上田一郎、玉田哲男: "植物ウイルスと媒介生物との相互関係・分子レベルからみた植物の耐病性"細胞工学別冊・植物細胞工学シリーズ8 秀潤社. 156-165 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Beet Necrotic Yellow Vein Virus, Data Sheet for CABI Crop Protection Compendium"CAB International, Wallingford, Oxon UK.. CD-ROM (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Benyviruses. Encyclopedia of Virology. 2nd Edition"Academic Press. Edition. 154-160 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Beet Necrotic Yellow Vein Virus"AAB Descriptions of Plant Viruses. UK (in press). CD-ROM (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Genome diversity of plant viruses transmitted by the plasmodiophorid fungi : gene function of BNYVV and its application. "The strategy for plant protection in physiological and molecular plant pathology""The phytoapthlogical Society of Japan. 36-44 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Lauber, E., Guilley, H., Tamada, T., Richards, K. E. and Jonard, G.: "Vascular movement of beet necrotic yellow vein virus in Beta macrocarpa is probably dependent on an RNA3 sequence domain rather than a gene product"J. Gen. Virol.. 79. 385-393 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Molecular and biological aspects of fungal transmission of viruses with rod-shaped particles"Invited Papers Abstracts-Vol 1, 7th International Congress of Plant Pathology. 1.13. 7S (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyanishi, M., Kusume, T., Saito, M., and Tamada, T.: "Beet necrotic yellow vein virus RNA5 : geographical distribution and sequence variation. Offered papers, Abstracts-Vol 2"7th International Congress of Plant Pathology. 1.11. 80 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saito, M., Kiguchi, T. and Tamada, T.: "Nonradioactive, digoxigenin-labeled DNA probes for the detection of five RNA species present in beet necrotic yellow vein virus"Bull. Res, Inst. Bioresour. Okayama Univ.. 5. 79-96 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyanishi, M., Kusume, T., Saito, M., and Tamada, T.: "Evidence for three groups of sequence variants of beet necrotic yellow vein virus RNA5"Arch. Virol.. 144. 879-892 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T., Uchino, H., Kusume, T., and Saito: "M. RNA3 deletion mutants of beet necrotic yellow vein virus do not cause rhizomania disease in sugar beets"Phytopathology. 89. 1000-1006 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T., Miyanishi, M., and Takemoto, M.: "Involvement of beet necrotic yellow vein virus RNA3 in restricted virus multiplication in resistant lines of Beta vulgaris ssp. maritima"Proc. Symp. 4th Int. Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyahishi, M., Kusume, T., Saito, M., and Tamada, T.: "Sequence variation of beet necrotic yellow vein virus RNA5 : the evolution and the possible route of its spread"Proc, Symp. 4th Int. Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirano, S., Kondo, H., Maeda, T., and Tamada, T.: "Burdock mottle virus has a high genome similarity to beet necrotic yellow vein virus"Proc. Symp. 4th Int. Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Beet necrotic yellow vein virus. Virology(Uirusugaku)"Asakurashoten. 435-442 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Uyeda, I. and Tamada, T.: "Plant virus-vector interactions. "Plant disease resistance : a new frontier in plant science" Shokubutsu Kogaku 8"Shujyunnsha. 156-165 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Beet Necrotic Yellow Vein Virus. Data Sheet for CABI Crop Protection Compendium"CAB International, Wallingford, Oxon, UK. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Benyviruses. Encyclopedia of Virology. Edited by R. G. Webster and A. Granoff. Second Edition"Academic Press. London. 154-160 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamada, T.: "Beet necrotic yellow vein virus"AAB Descriptions of Plant Viruses (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Tamada,H.Uchino,T.Kusume,M.Saito: "RNA3 deletion mutants of beet necrotic yellow vein virus do not cause rhizomania disease in sugar beets"Phytopathology. 89. 1000-1006 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Tamada,M.Miyanishi M.Takemoto: "Involvement of beet necrotic yellow vein virus RNA3 in restricted virus multiplication in resistant lines of Beta maritima"Proc.Symp.4th Int.Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Miyanishi,T.Kusume,M.Saito,T.Tamada: "Sequence Variation of beet necrotic yellow vein virus RNA5 : the evolution and the possible route of its spread"Proc.Symp.4th Int.Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Hirano,H.Kondo,T.Maeda,T.Tamada: "Burdock mottle virus has a high genome similarity to beet necrotic yellow vein virus"Proc.Symp.4th Int.Working Group on Plant Viruses with Fungal Vectors. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Tamada: "Benyviruses,Encyclopedia of Virology,2nd ed."Academic Press London. 7 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Tamada: "Beet necrotic yellow vein virus"AAB Descriptions of Plant Viruses,CD-ROM (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Tamada: "Molecular and biological aspects of fungal tranomission with vod-shaped particles" 7th International Congress of Plant Pathology.Invited paper Astrads. I. 1,13,75 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Miyanishi,T.Kusume,M.Saito,T.Tamada: "Beet neerotic yellow vein virus RNA5:geographical distribution and sequence variation" 7th International Congress of Plant Pathology.paper Offered Ahstrant. II. 1,11,80 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Miyanishi,T.Kusume,M.Saito,T.Tamada: "Evidence for three groups of sequence variants of beet nerotic yellow vein virus RNA5" Arch.Virol.144(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Tamada: "Benyviruses.Encyclopedia of Virology Znd ed.(in press)" Academic Press.London, (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Tamada: "Beet necrotic yellow vein virus.Data Sheet for CAB1 Crop Protection Compendium(CD-ROM)" CAB International.UK, (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 玉田哲男: "菌類が媒介する植物ウイルス遺伝子の多様性:分子機構とその応用" 日本植物病理学会 平成9年度植物感染生理談話会. 36-44 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Lauber,E., Guilley,H., Tamada et al: "Vascular movement of beet necrotic yellow vein virus in Beta macrocarpais probably dependent on an RNA 3 sequence domain rather than a gene product." Journal of General Virology. 79. 385-393 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 上田一郎・玉田哲男: "植物ウイルスと媒介生物との相互関係、分子レベルからみた植物の耐病性" 細胞工学別冊植物細胞工学シリーズ8.秀潤社, 156-165 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 玉田哲男: "ビ-トネクロテックイエロウベインウイルス ウイルス学" 朝倉書店, 435-442 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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