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Strain Establishment, Storage, and Application of the Fungivorous Nematode, Aphelenchus avenae.

Research Project

Project/Area Number 09460030
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionSAGA UNIVERSITY

Principal Investigator

ISHIBASHI Nobuyoshi  Saga University, Dept. Appl. Sci., Professor, 農学部, 教授 (30039333)

Co-Investigator(Kenkyū-buntansha) KONDO Eizo  Saga University, Dept. Appl. Sci., Professor, 農学部, 教授 (60039336)
TANAKA Kinji  Saga University, Agric. Fsrm, Professor, 農学部, 教授 (50039335)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordsfungivorous nematodes / Aphelenchus avenae / soil conditioner / biological control / soil-borne fungal diseases / mass-production / long-term preservation / application / 土壌妨害防除 / 糸状菌選好性 / 系統確立 / 整剤化 / 混合施用 / 総合防除 / 産業廃棄物
Research Abstract

1) Strain establishment : 39 isolates were mostly collected from Kyushu. Others were from Okinawa, Kanto, and Tohoku districts. These isolates were divided into 5 groups according to their host fungi preference, temperature optima for reproduction, and male occurrence. However, PCR-RLFP and PCR-RAPD analyses did not show any relevance to these preference or male occurrence. Only esterase isoenzyme phenotypes had a slight relevance to these traits. Host/temperature preferences may have stemmed from an adaptation or acclimatization.
2) Mass-production and long-term preservation The NEMATODES are mass-produced by feeding fungal hyphae on solid substrates of various industrial vegetable wastes in different combinations. The mixed medium of vegetable and animal wastes greatly enhanced the NENATODE multiplication. Addition of used green tea or tea plant debris (30%) is essential component of the medium. From initial 200 nematodes, 1.2 x 10ィイD16ィエD1 were harvested 20 days after inoculation on … More the mixed media. NEMATODES are also mass-produced on sponge substrates permeated with PDA solution as a nutrient source for the host fungus. The highest multiplication 1.1 x 10ィイD16ィエD1 was recorded on 20 g substrates 30 days after inoculation. The produced NEMATODES are put in a hollow pellet made from used medium that was spent for production of NEMATODES. The pellet containing NEMATODES 20,000 - 100,000 per pellet are placed in a drying chamber for about 30 hr. After the NEMATODES entered steady anhydrobiolic state, they can be stored in a room at ambient temperature for more than one year without loosing the viability.
3) Application : NEMATODES can be applied widely in farming crops for biological control of soil-borne fungal diseases of plants, and for integrated control of soil pests other than fungi by mixed application with other biocontrol agents. Dried pellets containing NEMATODES (20,000/pellet) are applied to a seedling pot with 200-300 ml soil for germination of seeds or propagation of seedlings or to field soil at the base of plants.
4) Patent application : Patents were applied to Japan, USA, EC, and Australia on the title "Mass-production, long-term preservation of the fungivorous nematode, Aphelenchus avenae, and uses thereof". In Japan and Australia, they were approved, and now on-going in USA and European Communities. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Ishibashi,N.,Saramoto,M.,& Ali,M.R.: "mass-production of fungivorous nematode,Aphelenchus avenae Bastian 1865,on industrial vegetable/animal wastes."Japanese Journal of Nematology. 30(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi,N.: "Possible integration of beneficial namatodes and microbial agents for biocontrol of soil pests."Japanese Journal of Nematology. 29(s.i). 22-28 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ali,M.R.,Buni,A.,& Ishibashi,N.: "Host and temperature preference,male occurrence and morphometrics of fungivorous nematode,Aphelenchus avenae isolates"Japanese Journal of Nematology. 29. 7-17 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ali,R.,Yamaguchi,Y.,& Ishibashi,N.: "RAPD-PCR and PCR-RFLP analysis on genetic diversity of Aphelenchus avenae isolates collected from Kyushu and other"Japanese Journal of Nematology. 29. 1-11 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 園田佳弘・田中欽二: "ヨシ堆肥によるタマネギ乾腐病抑制効果について"佐賀大学農学部付属農場報告. 11. 19-25 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo,E.,& Kaya,H.: "Recent development of biological control by beneficial namatodes : Synopsis and Discussion"Japanese Journal of Nematology. 28(s.i.). 75-78 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi,N.: "Behaviour of Entomopathogenic Nematodes. In : The Biology of Nematodes. Ed. by D. L. Lee"harwood academic publishers, Reading, UK.. 850 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 石橋信義: "「土壌の働きと根圏環境」土壌動物・線虫類(農業技術大系)"農文協 土壌肥料編(3月刊行). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ali, M. R., Buni Amin, Adachi, T., & Ishibashi, N.: "Host and temperature preference, male occurrence and morphometrics of fungivorous nematode, Aphelenchus avenae isolates from Japan"Japanese Journal of Nematology. 29. 7-17 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ali, M. R., Yamaguchi, Y., & Ishibashi, N.: "RAPD and PCR-RFLP analyses on genetic diversity of Aphelenchus avenae isolates collected from Kyushu and some other districts of Japan"Japanese Journal of Nematology. 29. 1-11 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ali, M. R., Yamaguchi, Y., & Ishibashi, N.: "Esterase isozyme phenotypes of 15 isolates of fungivorous nematode, Aphelenchus avenae Bastian 1865, from Kyushu and other districts of Japan"Japanese Journal of Nematology. 29. 12-18 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi, N.: "Possible integration of beneficial nematodes and microbial agents for biocontrol of soil pests"Jpn. J. Nematol. 29(Special Issue). 22-28 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi. N., Saramoto, M., & Ali, M. R.: "Mass-production of fungivorous nematode, Aphelenchus avenae Bastian 1865, on industrial vegetable/annmal wastes"Japanese Journal of Nematology. 30: (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sariah, M., & Tanaka, K.: "Effect of flooding on the viability and pathogenicity of scierotia of Sclerotium rofsii : in Malaysia soil"Int. J. Pest Management. 41. 97-99 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo, E.: "Infection mechanism and growth regulation in entomopathogenic nematodes, Steinerema spp."Biological Pest Control in Systems of Integrated Pest Management. FFTC Book Series. No. 47 (FFTC). 27-39 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo, E. & Kaya, H. K.: "Recent development of biological control by beneficial nematodes : Synopsis and Discussion"Japanese Journal of Nematology. 28(Special Issue). 75-78 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo, E.: "Infection mechanism and growth regulation in enotmopathogenic nematodes, Steinernema spp."Biological Pest Control in Systems of Integrated Pest Management. FFTC. 27-39 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi, N.: "Behaviour of Entomopathogenic Nematodes. In : The Biology of Nematodes"harwood academic publishers, Reading, UK.. Ca. 850 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi, N.: "Mineralization of nutrients : The role of soil invertebrates ; Protozoans, Enchytraeidae, and Nematodes"Soil & Fertilizer, NOBUNJYO, March Issue. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi, N.: "Insect Pathology ; Diseases by Nematodes In : Insect - Basis & Practice. Ed. By Mitsuhashi, J."Asakura Book publisher. 800. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishibashi,Nobuyoshi: "Possible integration of beneficial nematodes and microbial agents for biocontrol of soil pests." Japanese Journal of Nematology. 28(suppl). 28-35 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishibashi,N.& X.D.Wang: "Infection behavior for species maintenence in steinernematid nematodes." Proceedings of VIIth International Colloquium of Invertebrate Pathology and microbial control.sapporo. 28-32 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ali.Md.Rustom and N.Ishibashi: "The fungivorous nematode,Aphelenchus avenae,isolates from kyushu,Japan,and their host preference etc." Japanese Journal of Nematology. 29(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishibashi,N.and Md.R.Ali,& M.Saramoto: "Mass-production of fungivorous nematode,Aphelenchus avenae,on industrial wastes or by-products of plant and animal origin" Japanese Journal of Nematology. 29(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ali.Md.Rustom and N.Ishibashi: "genetic diversity among isolates of fungivorous nematode,Aphelenchus avenae,from Kyushu and Okinawa in Japan." Japanese Journal of Nematology. 29(発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] Wang,X.D.and N.Ishibashi: "Infectivity of the entomopathogenic nematode,Steinernema carpocapsae,as affected by the presence of S.glaseri." Journal of Nematology(USA). 31(印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishibashi,Nobuyoshi: "Behaviour : Behaviour of Entomopathogenic Nematodes" Hewitt Publisher UK, 800-1000 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 石橋信義: "昆虫病原性線虫の利用" バイオコントロール. 1(1). 3-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 石橋信義: "菌食性線虫Aphelenchus avenaeによる土壌病害の防除" バイオコントロール. 2(1). 8-13 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wang,X.D.,E.Kondo,and N.Ishibashi: "Infectivity of entomopathogenic nematode, Steinernema carpocapsae,as affected by the emergence time from the host cadaver." Japanese Journal of Nematology(日本線虫学会誌). 27・1. 1-6 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Waug,X.D.and N.Ishibashi: "Effects of the precedent infection of the entomopathogenic nematodes,Steinernematidae,on the subsequent invasion of the following infectives." Japanese Journal of Nematology (日本線虫学会誌). 28(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ali,R.,E.Kondo,& N.Ishibashi: "Effect of temperature on the development and reproduction of D.angustus on fungal mat of Botrytis cinerea." Jpn.J.Nematol.(日本線虫学会誌). 27. 7-13 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ali,R.,Y.Fukutoku,& N.Ishibashi: "Effect of Ditylenchus angustus on the growth of the rice plant" Jpn.J.Nematol.27. 51-65 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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