| Project/Area Number |
09460034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | KYOTO INSTITUTE OF TECHNOLOGY |
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Principal Investigator |
SUGIMURA Yukio Faculty of Textile Science, Kyoto Institute of Technology, Associate Professor, 繊維学部, 助教授 (20273542)
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| Co-Investigator(Kenkyū-buntansha) |
KOTANI Eiji Faculty of Textile Science, Kyoto Institute of Technology, Research Associate, 繊維学部, 助手 (10273541)
FURUSAWA Toshiharu Faculty of Textile Science, Kyoto Institute of Technology, Professor, 繊維学部, 教授 (70127166)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Mulberry / Plantlet regeneration / Gene delivery / Transformant / Molecular farming / 個体再生系 |
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| Research Abstract |
Mulberry plant, a woody feedcrop for the silkworm, is an attractive species for molecular framing since the plant have superior agronomical characteristics. Transgenic mulberry clone, if produced, may provide a farming system for the production of valuable biomolecules. To realize this target, it is essential to establish the methods for (1) plantlet regeneration from explant tissues and (2) gene delivery into target tissue. High frequency of shoot regeneration was achieved by 2 step-culture system consisting of seedling culture for obtaining leaf explants and leaf culture for inducing shoot organogenesis. A critical factor in both cultures was medium composition in which 2, 3, 5-triiodobenzoic acid and cytokinin such as 6-benzylaminopurin and thidiazuron was included. For efficient gene delivery into regenerable leaf tissue, various bombardment conditions were optimized using a particle inflow gun device : gold particle as microprojectile, bombardment frequency, preculture period and conditioning treatment of target tissue. When the optimal conditions were combined, 200-300 gene expressing-cells per cmィイD12ィエD1 tissue were detected. Based on these results, it is possible to generate transgenic mulberry clones.
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