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Study on the function of laminin by producing transgenic mice and fruit flies

Research Project

Project/Area Number 09460048
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionNagoya University

Principal Investigator

KITAGAWA Yasuo  Nagoya University Bioscience Center, Professor, 生物分子応答研究センター, 教授 (50101168)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordsorganogenesis / basement membrane / Drosophila / mouse / laminin / ショウジョウバエ
Research Abstract

Laminins are heterotrimers with alpha, beta, and gamma chains held together by a triple-stranded alpha-helical coiled-coil structure. This structure is highly conserved among laminins from mammalians and invertebrates. Various isoforms of laminin chains have been found and this diversity raised the question how laminin chains are selected for the heterotrimer assembly. We here studied in vivo process of the heterotrimer assembly using four experimental systems. First, we expressed mouse laminin alpha1, beta1 or gamma1 sequence covering various region of the long arm in monkey COS1 cells and found that a common mechanism is shared by laminin chains of different animal origins. We also found that the sequences in E8 domain at C-terminal end of the long arm are crucial for the assembly. Second, we expressed mouse laminin beta1 covering either C-terminal end or whole of the long arm in mouse embryonal carcinoma F9 cells. Both fragments were disulfide-bonded to endogenous gamma1 and formed trimers with alpha1, which was actively secreted into the medium. However, beta1 sequence covering C-terminal end could not support the disulfide-bonding of alpha1 at N-terminus of the long arm. Third, we developed chain specific antibodies directed against Drosophila laminin alpha, beta and gamma and found that the disulfide-bonding between Drosophila beta and gamma is essential for alpha to from alphabetagamma heterotrimer. Fourth, we found that laminin-8 with chain composition of alpha4beta1gamma1 is the only isoform expressed in 3T3-L1 adipocytes. Northern blot showed the levels of alpha4, beta1 and gamma1 mRNAs increase 2.5-fold during the adipose conversion of the cells.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Tomoaki Niimi,Yasuo Kitagawa: "Distinct roles of mouse laminin β1 long arm domains for α1β1γ1 trimer formation." FEBS Letters. 400. 71-74 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoaki Niimi,Kiyoshi Miki,and Yasuo Kitagawa: "Expression of the long arm sequence of mouse laminin α1,β1 or γ1 chain in COS1 cells and assembly of monkey-mouse hybrid laminin." J.Biochem.121. 854-861 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chino Kumagai,Tatsuhiko Kadowaki,and Yasuo Kitagawa: "Disulfide-bonding between Drosophila laminin β and β chains is essential for a chain to form αβγ trimer." FEBS Letters. 412. 211-216 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoki Niimi,Chino Kumagai,Masaki Okano,and Yasuo Kitagawa: "Differentiation-dependent expression of laminin-8 (α4β1γ1) mRNA in mouse 3T3-L1 adipocytes." Matorix Biology. 16. 223-230 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi,Kazuhiro Torivama,Eleni Nicodemou-Lena,Kazuhiko Inou,Shuhei Torii,and Yasuo Kitagawa: "De novo adipogenesis in mice at the site of injection of basement membrance and basic fibroblast growth factor." Proc.Natl.Acad.Sci.USA. 95. 1062-1066 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 鳥山和宏、川口信子、鳥居修平、北川泰雄: "脂肪組織の工学" 組織培養工学. 24(4). 143-146 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuo Kitagawa,Tomoaki Niimi,and Chino Kumagai: "Role of interchain disulfide-bonding in dimer-trimer switch during laminin heterotrimer assembly" Extracellular Matrix-Cellular Interaction : Molecules to Diseases (Y.Ninomiya et al.,eds.), 29 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 熊谷知乃、川口信子、北川泰雄: "ラミニンの分離・同定法,細胞外マトリックス研究法〔1〕" 畑隆一郎、服部俊治、新井克彦編、コラーゲン研究会刊, 12 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoaki Niimi, Yasuo Kitagawa: "Distinct roles of mouse laminin beta1 long arm domains for alpha1beta1gamma1 trimer formation." FEBS Letters. 400. 71-74 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoaki Niimi, Kiyoshi Miki, and Yasuo Kitagawa: "Expression of the long arm sequence of mouse laminin alpha1, beta1 or gamma1 chain in COS1 cells and assembly of monkey-mouse hybrid laminin." J.Biochem.121. 854-861 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chino Kumagai, Tatsuhiko Kadowaki, and Yasuo Kitagawa: "Disulfide-bonding between Drosophila laminin beta and beta chains is essential for a chain to form alphabetagamma trimer." FEBS Letters. 412. 211-216 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoaki Niimi, Chino Kumagai, Masaki Okano, and Yasuo Kitagawa: "Differentiation-dependent expression of laminin-8 (alpha4beta1gamma1) mRNA in mouse 3T3-L1 adipocytes." Matorix Biology. 16. 223-230 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi, Kazuhiro Toriyama, Eleni Nicodemou-Lena, Kazuhiko Inou, Shuhei Torii, and Yasuo Kitagawa: "De novo adipogenesis in mice at the site of injection of basement membrance and basic fibroblast growth factor." Proc.Natl.Acad.Sci.USA. 95. 1062-1066 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuo Kitagawa, Tomoaki Niimi, and Chino Kumagai: "Role of interchain disulfide-bonding in dimer-trimer switch during laminin heterotrimer assembly." Extracellular Matrix-Cellular Interaction : Molecules to Diseases (Y.Ninomiya et al., eds.). 41-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoaki Niimi,Yasuo Kitagawa: "Distinct roles of mouse laminin β1 long arm domains for α1β1γ1 trimer formation." FEBS Letters. 400. 71-74 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tomoaki Niimi,Kiyoshi Miki,and Yasuo Kitagawa: "Expression of the long arm sequence of mouse laminin α1,β1 or γ1 chain in COS1 cells and assembly of monley-mouse hybrid laminin." J.Biochem.121. 854-861 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chino Kumagai,Tatsuhiko Kadowaki,and Yasuo Kitagawa: "Disulfide-bonding between Drosophila laminin β and β chains is essential for a chain to form αβγ trimer." FEBS Letters. 412. 211-216 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tomoaki Niimi,Chino Kumagai,Masaki Okano,and Yasuo Kitagawa: "Differentiation-dependent expression of laminin-8(α4β1γ1)mRNA in mouse 3T3-L1 adipocytes." Matorix Biology. 16. 223-230 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuko Kawaguchi,Kazuhiro Torivama Eleni Nicodemou-Lena,Kazuhiko Inou,Shuhei Torii,and Yasuo Kitagawa: "De novo adipogenesis in mice at the site of injection of basement membrance and basic fibroblast growth factor." Proc.Natl.Acad.Sci.USA. 95. 1062-1066 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鳥山和宏、川口信子、鳥居修平、北川泰雄: "脂肪組織の工学" 組織培養工学. 24(4). 143-146 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasuo Kitagawa,Tomoaki Niimi,and Chino Kumagai: "Role of interchain disulfide-bonding in dimer-trimer switch during laminin heterotrimer assembly." Extracellular Matrix-Cellular Interaction:Molecules to Diseases(Y.Ninomiya et al.,eds.), 29 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 熊谷知乃、川口信子、北川泰雄: "ラミニンの分離・同定法,細胞外マトリックス研究法[1]" 畑隆一郎、服部俊治、新井克彦編、コラーゲン研究会刊, 12 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tomoaki Nimimi Yasuo Kitagawa: "Distinct roles of mouse laminin β1 long arm domains for α1β1γ1 trimer formation" FEBS Letters. 400. 71-74 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomoaki Niimi Kiyoshi Miki Yasuo Kitagawa: "Expression of the long arm sequence of mouse laminin α1, β1, or γ1 chain in COS1 cells and assembly of monkey-mouse hybrid laminin" J.Biochem.121. 854-861 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Chino Kumagai, Tatsuhiko Kadowaki Yasuo Kitagawa: "Disulfide-bonding between Drosophila laminin β and γ chains is essential for α chain to form α β γ trimer" FEBS Letters. 412. 211-216 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomoaki Niimi., Chino Kumagai Masaki Okano., Yasuo Kitagawa: "Differentiation-dependent expressoin of laminin-8 (α4β1γ1) mRNAs in mouse 3T3-L1 adipocytes" Matrix Biology. 16. 230-223 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 新美友章, 熊谷知乃, 北川泰雄: "特集 マトリックス生物学の最前線:多様なラミニン分子の形態機構" 生体の科学. 48. 260-267 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nobuo Kawaguti Yasuo Kitagawa., et al.: "De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor" Proc.Natl.Acad.Sci.USA. 95. 1062-1066 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 熊谷知乃, 川口信子, 北川泰雄: "細胞外マトリックス研究法-基礎知識からデーターの解釈まで-" コラーゲン技術研修会(畑隆一郎,服部俊治,新井克彦 編), 600 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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