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Studies on the structure and rearrangements of the linear chromosome of Streptomyces griseus 2247.

Research Project

Project/Area Number 09460050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KINASHI Haruyasu  Graduate School of Advanced Sciences of Matter, Department of Molecular Biotechnology, Professor, 大学院・先端物質科学研究科, 教授 (80224997)

Co-Investigator(Kenkyū-buntansha) SHINKAWA Hidenori  Graduate School of Advanced Scienc-es of Matter, Department of Molecular Biotech, 大学院・先端物質科学研究科, 助教授 (50226338)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥10,800,000 (Direct Cost: ¥10,800,000)
Keywordsactinomycetes / Streptomyces / linear chromosome / genome project / terminal sequence / chromosomal deletion / chromosomal circularization / chromosomal recombination
Research Abstract

The structure of the linear chromosome of Streptomyces griseus strain 2247 and its dynamic rearrangements have been studied on molecular level, and the following results were obtained.
1. The nucleotide sequence of the chromosomal end of S.griseus 2247 was determined and compared with those of other Streptomyces linear chromosomes and plasmids. The conserved end sequence was not found there but several inverted repeats were. These sequences may form hairpin loops and function in replication of the linear DNA.
2. Many deletion mutants have been prepared by UV-irradiation of strain 2247. The relationship between the deletion size and topology of the mutant chromosomes suggests that some region near the afsA gene on the left side of the chromosome is essential for maintenance of the linear chromosome.
3. The fusion junctions of the circularized chromosomes in three mutants, 404-23, N2 and 83, were cloned and sequenced. The chromosomal circularization was deduced to occur by illegitimate recombination between the right and left deletion ends which do not have any homology to each other.
4. Only the mutant 301-22 keeps a linear chromosome. Complicated rearrangements were found to have occurred on its chromosome. Namely, the right arm of the chromosome was replaced by the left arm, and additionally a new fragment was attached to both deletion ends of the chromosome.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] A.Lezhava: "Chromosomal deletions in Streptomyces griseus that remove the afsA locus" Molecular General Genetics. 253. 478-483 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Zhang: "Improvement of transformation system in Streptomyces using a modified regeneration medium." Journal of Fermentation and Engineering. 83. 217-221 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Reolenbach: "Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCP1" Journal of Bacteriology. 180. 2796-2799 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Kinashi: "Physical mapping of the linear plasmid pSLA2-L and localization of the eryAI and actI homologs" Bioscience,Biotechnology and Biochemistry. 62. 1892-1897 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ueda: "Characterization of an A-factor-responsive represser for amfR essential for onset of aerial mycelium-formation in Streptomyces griseus" Journal of Bacteriology. 180. 5085-5093 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 木梨陽康: "放線菌の線状染色体のダイナミックな構造変化と染色体の起源・進化" 蛋白質核酸酵素. 43. 1453-1460 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 木梨陽康: "バイオテクノロジー講義(分担執筆)" 朝倉書店, 156 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Lezhava: "Chromosomal deletions in Streptomyces griseus that remove the afsA locus." Mol.Gen.GEnet.253. 478-483 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Zhang: "Improvement of transformation system in Streptomyces using a modified regeneration medium." J.Ferment.Bioeng.83. 217-221 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Redenbach: "Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCP1." J.Bacteriol.180. 2796-2799 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Kinashi: "Physical mapping of the linear plasmid pSLA2-L and localization of the eryAI and actI homologs." Biosci. Biotech. Biochem.62. 1892-1897 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ueda: "Characterization of an A-factor-responsive repressor for amfR essential for onset of aerial mycelium formation in Streptomyces griseus." J.Bacteriol.5085-5093 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Kinashi: "Dynamic structural changes of the linear chromosomes of Streptomyces and the origin and evolution of chromosomes." Tanpakushitsu Kakusan Koso. 43. 1453-1460 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Kinashi: "Linear chromosomes and plasmids in Streptomyces, bacteria which produce antibiot-ics." In Lectures of Biotechnology, Asakura Shoten, tokyo. 42-49 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Redenbach: "Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCP1." Journal of Bacteriology. 180. 2796-2799 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Kinashi: "Physical mapping of the linear plasmid pSLA2-L and localization of the eryAI and actI homologs." Bioscience,Biotechnology and Bio chemistry. 62. 1892-1897 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Ueda: "Characterization of an A-factor-responsive repressor for amfRessential for onset of aerial mgcelium formation in Streptomyces griseus." Journal of Bacteriology. 180. 5085-5093 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 木梨陽康: "放線菌の線状染色体のダイナミックな構造変化と染色体の起源・進化" 蛋白質核酸酵素. 43. 1453-1460 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Lezhava: "Chromosomal deletions in Streptomyces griseus that remove the afsA locus." Molecular General Genetics. 253. 478-483 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Redenbach: "Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCP1" Journal of Bacteriology. 180. in press (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Zhang: "Improvement of transformation system in Streptomyces using a modified regeneration medium" Journal of Fermentation and Bioengineering. 83. 217-221 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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