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Lyso-sphingolipid forming enzyme and physiological functions of lyso-sphingolipids

Research Project

Project/Area Number 09460051
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

ITO Makoto  Kyushu University, Agriculture, Associate Professor, 農学部, 助教授 (40253512)

Co-Investigator(Kenkyū-buntansha) HIGASHI Hideyoshi  Mitsubishi Kasei Institute of Life Sciences, Senior researcher, 生命科学研究所, 主任研究員
沖野 望  九州大学, 大学院・農学研究科, 日本学術振興会特別研
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 1998: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1997: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordssphingolipid / lyso-sphingolipid / SCDase / apoptosis / neuron / ganglioside / sphingolipidosis / caspase / ガグクオシド / Neuro.Zo / 加水分解の逆反応(縮合反応) / 遺伝子クローニング / リゾスフィンゴミエリン / 糖脂質の脂質工学
Research Abstract

Sphingosylphosphorylcholine, lyso-GM1 and lyso-GM2, all of which are Mdeacylated derivative of the parental sphingolipids, were known to accumulate in the brain of neuropathic sphingolipidosis patients of Niemann-Pick disease type A, GM1 gangliosidosis, Tay-Sachs and Sandhoff diseases, respectively. This study described that these lysosphingolipids can induce apoptosis in mouse neuroblastoma Neuro2a. Lysosphingolipids, prepared by enzymatic N-deacylation of sphingolipids by SCDase, inhibited [^3H]thymidine incorporation of these cells in a dose dependentmanner, while the parental sphingolipids had no effect. It was revealed that the lysosphingolipid-dependent inhibition of mitogenesis was not due to the differentiation but to the induction of apoptosis of the cells. When Neuro2a cells were cultured with 40-80 muM lysosphingolipids for 48 h, intranucleosomal DNA fragmentation and chromatin condensation were observed. These results may support an idea that lyso-sphingolipids are candidate for etiologic factors of sphingolipidosis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Susumu Mitsutake: "Synthesis of ^<14>C-labeled caramide by fatty acid transfer reaction of sphigolipid ceramideN-deacylase" Analytical Biochemistry. 247. 52-57 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Susumu Mitsutake: "Enzymatic synthesis of [^<14>C]-glycosphingolipids by reverse hydrolysis reaction of sphingolipid ceramide N-deacylase" Journal of Biochemistry. 123. 859-863 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nozomu Okino: "Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa." Journal of Biological Chemisty. 273・23. 14368-14373 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Motohiro Tani: "Enzymatic synthesis of ω-amino-ceramide: Preparation of a sensitive fluoresecent substrate for ceramidase." Analytical Biochemistry. 263. 183-188 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hironobu Komori: "Regulation of intracellular ceramide content in B16 melanoma cells." Journal of Biological Chemisty. 274印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Makoto Ito: "Enzymatic N-deacylation of sphingolipids." Methods in Enzymology. 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsutake, S., Kita, K., Okino, N.and Ito, M.: "Synthesis of ^<14>C-labeled ceramide by fatty acid transfer reaction of sphigolipid ceramideN-deacylase and its application for assay of ceramidase." Anal.Biochem.247. 52-57 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsutake, S., Kita, K., and Ito, M.: "Enzymatic synthesis of [^<14>C]-glycosphingolipids by reverse hydrolysisreaction of sphigolipid ceramideN-deacylase." J.Biochem.123. 859-863 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okino, N., Tani, T., Imayama, S., and Ito, M.: "Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa." J.Biol.Chem.273. 14368-14373 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tani, M., Kita, K., Komori, H., and Ito, M.: "Enzymatic synthesis of omega-amino-ceramide : Preparation of a sensitivefluoresecent substrate for ceramidase." Anal.Biochem.263. 183-188 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Komori, H., Ichikawa, S., and Ito, M.: "Regulation of intracellular ceramide content in B16 melanoma cells." J.Biol.Chem.274 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito, M., Kita, K., Kurita, and Izu, H.: "Enzymatic N-deacylation of sphingolipids." Methodin Enzymology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Susumu Mitsutake: "Synthesis of ^<14>C-labeied ceramide by fatty acid transfer reaction of sphigolipid ceramideN-deacylase" Analytical Biochemistry. 247. 52-57 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Susumu Mitsutake,: "Enzymatic synthsis of (^<14>C)-glycosphingolipids by reverse hydrolysis reaction of sphingolipid ceramide N-deacylase" Fournal of Biochemistry. 123. 859-863 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nozomu Okino: "Purification and characterization of a novel ceramidase from Pseukomonas aeruginosa." Journal of Biological Chemistry. 273・23. 14368-14373 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Motohiro Tani: "Enzymatic synthesis of w-amino-ceramide:Preparation of a sensitive fluoresecent substrate for ceramidase." Analytical Biochemistry. 263. 183-188 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hironobu Komori: "Regulation of intracellular ceramide content in B16 melanoma cells." Journal of Biological Chemistry. 274(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Makoto Ito: "Enzymatic N-deacylation of sphingolipids." Methods in Enzymology. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroyuki Izu: "Molecular cloning expression, and sequence analysis of the endoglycoceramidase II gene from Rhodococcus species strain M-777" The Journal of Biological Chemistry. 272・32. 19846-19850 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Noriyuki Sueyoshi: "Preparation of a naturally occurring D-erythro-(2S,3R)-sphingosylphosphocholine using Shewanella alga NS-589" Journal of Lipid Research. 38. 1923-1927 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasufumi Fukano: "Preparation of GM1 ganglioside with sialidase-producing marine bacteria as a microbial biocatalyst" Applied and Environmental Microbiology. 63・5. 1861-1865 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Susumu Mitsutaka: "[^<14>C]Ceramide synthesis by sphingolipid ceramide N-deacylase:New assay for ceramidase activity detection" Analytical Biochemistry. 247. 52-57 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 伊東 信: "新しい酵素を用いたスフィンゴ脂質工学" バイオサイエンスとインダストリー. 55・5. 11-15 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 伊東 信: "糖鎖付加による細胞内セラミド量の調節機構" 実験医学. 15・12. 1476-1481 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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