| Project/Area Number |
09460054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SUGIYAMA Takeyoshi Tohoku Univ.Dept.of Applyed Biol.Chem.Associate Professor., 農学部, 助教授 (10125586)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Toshihiko Akita Natl.Coll.Technol.Dept.of Chem.Biol.Engineering, Professor., 物質工学科, 教授 (00089799)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1998: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1997: ¥7,700,000 (Direct Cost: ¥7,700,000)
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| Keywords | enacyloxin / inhibition of protein biosynthesis / antibiotics / absolute stereochemistry / organic synthesis / biosynthesis-related substance / Frateuria sp.W-315 |
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| Research Abstract |
Enacyloxins (ENXs) are antibiotics produced by bacteria Frateuria sp. W-315 in a spent medium of Neurospora crassa. They are active against Gram-positive and Gram-negative bacteria Their mode of action is considered to be the inhibition of protein biosynthesis of susceptible strains. To elcidate the absolute stereochemistry of EM, ENX IIa derivative was oxidized with periodate and one of the products were compared with authentic samples which were derived from D-glucose. The stereochemistry of some of chiral centers were determined as 12'S, 17'R,18'S,19'R.The stereochemistry of other chiral centers are studied now.
As to biosynthesis of ENX, acidic compound, which appearance and disappearance are closely related to the biosynthetic status of EM, was newly found in the culture medium. To elucidate the chemical structure of the above acidic compound, the production and pufication are in progress.
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