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Development and mode of action of inhibitors of macromolecule biosynthesis from marine animals.

Research Project

Project/Area Number 09460057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

IKEGAMI Susumu  Hiroshima University, Dept.Applied Biochemistry, Professor, 生物生産学部, 教授 (80011980)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsStarfish / Sponge / Blastula / Gastrula / Fertilization / Hatching / Development / Metallo-endoproteinaae / 胚 / 阻害 / メタロエンドプロティナーゼ / アンコリノシド / テトラシン酸
Research Abstract

During the course of our screening program for inhibitors of starfish (Asterina pectinifera) embryonic development from marine organisms, we found that the MeOH extract of the marine sponge Rhopaloeides sp. inhibited starfish embryogenesis specifically at the stage prior to gastrulation. A preliminary examination led to the isolation of an unusual norsesterterpene designated rhopaloic acid A which inhibited gastrulation of the starfish embryo and exhibited potent cytotoxicities against human myeloid K-562 cells, human MOLT-4 leukemia cells, and murine L1210 leukemia cells7. Further investigation of the extract of Rhopaloeides sp. led to the isolation of related metabolites, rhopaloic acids B and C.We determined the structure and uncovered biological activities of these compounds.
We discovered a novel nucleic-acid associated protein designated as NAAP which was isolated from starfish oocytes. Oligonucleotides derived from the partial amino acid sequences of NAAP were used to clone NAAP cDNA and the entire cDNA sequence was determined. The transcript encoding NAAP was present only in growing oocytes but not in full-grown oocytes, embryos and adult tissues. The NAAP protein localized in the germinal vesicle of the full-grown oocyte and dispersed diffusely after germinal vesicle breakdown at the onset of meiosis. During oocyte maturation, NAAP changed the molecular form in a cell-cycle dependent manner. It was found that Ser 145 of NAAP, which was unphosphorylated at the germinal vesicle stage, was phosphorylated during the M phase of meiosis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 加藤宏一(K.H.Kato): "Inhibition of sea urchin fertilization by jaspisim,a novel Anti-metallo-endoproteinase substance" Development,Growth&Differentiation. 40巻. 221-230 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 毛利達磨(T.Mohri): "Sperm-induced local [Ca^<2+>] rise separated from the Ca^<2+> wave in sea urchin eggs in the presence of a gamete fusion inhibitor,jaspisin" Development. 125巻. 293-300 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 楊井 美穂子(M.Yanai): "Novel norsesterterpenes,which inhibit gastrulation of stan-fish embryo,from the masine sponge Rhopdloiedes sp." Tetrahedron. 54巻. 15607-15612 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 高尾敏文(T.Takao): "High-sensitivity mass spectromitry for analysis of post-translational modification" J.Protein Chemistry. 16巻. 409-413 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 太田伸二(S.Ohta): "Ancorinoside A:a novel tetramic acid glycoside from the marine sponge,Anocorinasp.,which specifically inhibits blashrlation" J.Organic Chemistry. 62巻. 6452-6453 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 清水孝彦(T.Shimizu): "Structure of a covalently cross-linked form of corehistones present in the starfish sperm." Biochemistry. 36巻. 12071-12079 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kato, K.H.et al.: "Inhibition of sea urchin fertilization by jaspisin, a novel anti-metallo-endoproteinase substance." Devlopment, Growth & Differentiation. 40. 221-230 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mohri, T.et al.: "Sperm-induced local [Ca^<2+>]_i rise separated from the Ca^<2+> wave in sea uchin eggs in the presence of a gamete fusion inhibitor, jaspisin." Develpoment. 125. 293-300 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yanai, M.et al.: "Novel sesterterenes, which inhibit gastrulation of starfish embryos, from yhe marine sponge, Rhopaloiedes sp." Tetrahedron. 54. 15607-15612 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takao, T.et al.: "High-sensitivity mass spectrometry for analysis of post translational modifications." J.Protein Chem.16. 409-413 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohta, S.et al.: "Ancorinoside A : a novel tetramic acid glycoside from the marine sponge, Ancorina sp., which specifically inhibits blastulation of starfish embryos." J.Org.Chem.62. 6452-6453 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimizu, T.et al.: "Structure of covalently cross-linked form of core histones present in the starfish sperm." Biochemistry. 36. 12701-12709 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Ohta,E.Ohta & S.Ikegami: "Novel norsesterterpenes,which inhibit gastrulation Z the starfish embryo,hom the marine sponge,Rhopaloiedes SP." Tetrahedron. 54. 15607-15612 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Hioki,M.Hamano,Y.Mimura M.Kodama,S.Ohta.M.Imai Y S.Ikegami: "Revised structure,synlhesis and aeoalute configurtaion of hippospongic acid A" Tetrahedron Letters. 39. 7745-7746 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Mohri,S.Miyazaki,H.Shirakawa & S.Ikegami: "Sperm-induced local [Ca^<2+>]rise separated from the Ca^<2+> wave in sea urchin eggs in the snesence of gamete finsim inhibitor jaspisin" Development. 125. 293-300 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.H.Kato,K.Takemoto,E.Kato,K.Miyazaki,H.Kobayashi & S.Ikegami: "Inhibition of sea urchin dertilization by jaspisin,a specific inhibiter of matrix metalloeneloproteinase" Development,Growth & Differentiation. 40. 221-230 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Ohta, E.Ohta & S.Ikegami: "Ancorinoside A : A novel tetramic acid glycoside from the marine Sponge,Ancorina sp.which Specifically inhibits blastulatim 7 sterlish embryo" Journal of Organic Chemistry. 62 (19). 6452-6453 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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