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Studies on the food factors that modulate transport and signal-converting functions of intestinal epithelial cell layer

Research Project

Project/Area Number 09460060
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionThe University of Tokyo

Principal Investigator

SHIMIZU Makoto  Graduate School of Agricultural and Life Science, The University of Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (30114507)

Co-Investigator(Kenkyū-buntansha) WATANABE Hirohito  Graduate School of Agricultural and Life Science, The University of Tokyo, Research associate, 大学院・農学生命科学研究科, 助手 (20270895)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥7,100,000 (Direct Cost: ¥7,100,000)
Keywordsfunctional food / food factor / intestinal absorption / transporter / tight junction / intestinal epithelial cell / Caco-2 / タイトジャクション
Research Abstract

The intestinal epithelium has three major functions, (1) barrier function against orally administered, toxicants or xenobiotics, (2) transport/absorption functions for the uptake of nutrients and functional food substances, and (3) transduction/conversion functions to receive food-derived signals and transfer the information into the body. This suggests that the intestinal epithelium is an important tissue which determines the bioavailability of food-derived functional substances. We have observed, by using an in vitro system with a human intestinal cell line Caco-2, that the intestinal functions could be affected by certain food factors. This study was carried out to reveal the food substances which could modulate the intestinal functions. As an example of food factors that could modulate intestinal barrier functions, we found that a protein from Enokitake (Flammulina velutipes) increased the permeability of the intestinal Caco-2 cell monolayer by opening intracellular tight junctions. This protein, termed TDP, was purified and its cDNA was cloned and sequenced.
TDP was a protein of 31kD and the mechanism for its action was revealed. As transporter-modulatory substances, we found that tea catechins (ECg and EGCg) and lysophosphatidylcholine (LPC) from sesame inhibited intestinal glucose and taurine transporters, respectively. Tea catechins were observed to inhibit glucose transporters (SGLT1 and GLUT) in a competitive manner. On the other hand, LPC was likely to affect the taurine transporter by interacting with lipid bilayers of the cell membrane as well as with the transporter molecule itself. The evidence cleary showing that the food signals from apical side were transferred to the basal side has not yet been obtained. However, recent experiments suggest that certain food substances added to the apical side of the Caco-2 monolayer could induce the secretion by Caco-2 cells of some bioactive substances to the basal side.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] H. Watanabe: "Purification and CDNA cloning of a protein derived from Flammaline velutipes that increases the permeability of the intestinal Caco-2 cell monolayer"Eur. J. Biochem.. 262. 850-857 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Satsu: "Hypertonicity stimulated taurine uptoke and transporter gene expression in Caco-2 cells"Biochim. Biophys. Acta. 1419. 89-96 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Shimizu: "Modulation of intestinal functions by food substances"Nahrung. 43. 154-158 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Ishizuakaら: "Taurine transport inhibitory substance in sesame seed"Biosci,Biotechnol.Biochem. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Shimizuら: "Regukation of intestinal glucase transport by tea catechins"Proc,2nd Intern,Confer.Food Factors. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Watanabe, A. Narai and M. Shimizu.: "Purification and cDNA cloning of a protein derived from Flammulina velutipes that increases the permeability of the intestinal Caco-2 cell monolayer."Eur. J. Biochem.. 262. 850-857 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Satsu, Y. Miyamoto and M. Shimizu: "Hypertonicity stimulated taurine uptake and transporter gene expression in Caco-2 cells."Biochim. Biophys. Acta. 1419. 89-96 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Shimizu: "Modulation of intestinal functions by food substances."Nahrung/Food. 43. 154-158 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Ishizuka, A. kanayama, H. Satsu, K. Furihata, Y. Miyamoto: "Taurine transport inhibitory substance in sesame seed."Biosci. Biotechnol. Biochem.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Shimizu, Y. Kobayashi, M. Suzuki, H. Satsu and Y. Miyamoto: "Regulation of intestinal glucose transport by tea catechins."Proceedings for the International conference of Food Factors. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Ogawa ら: "Acetic acid suppresses the increase of disaccharidase activity during cultute of caco-2 intestinal epithelial cells"J. Nutr.. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Shimizu and H. Satsu: "Physiological significance of taurine and the taurine transporter in intestinal epithelial cells"Amino Acids. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Ishizuka ら: "A taurine transport inhibitory substance in sesame seeds"Biosci. Biotechnol. Biochem.. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Shimizu: "Modulation of intestinal functions by food substances" Nahrung/Food. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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